Human GHRHR Antibody Summary
Accession # Q02643
Detection of GHRHR in Transfected HEK293 (positive) and Wild type (negative) Cells. GHRHR was detected in immersion fixed transfected HEK293 (positive) and wild type (negative) cells using Rabbit Anti-Human GHRHR Monoclonal Antibody (Catalog # MAB107353) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of GHRHR in Human Pituitary. GHRHR was detected in immersion fixed paraffin-embedded sections of human pituitary using Rabbit Anti-Human GHRHR Monoclonal Antibody (Catalog # MAB107353) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
GHRHR is a GPCR that binds growth hormone-releasing hormone that is expressed in the pituitary. Significant amounts of GHRHR are also found in the hypothalamus, kidney, and placenta. GHRH regulates the secretion of growth hormone that controls the metabolism and growth of every tissue through its binding with GHRHR. Malfunction in GHRHR signaling is associated with abnormal growth. GHRHR is a therapeutic target against dwarfism, gigantism, lipodystrophy, and certain cancers.
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