|Detection of Human GIF by Western Blot. Western blot shows lysates of human stomach tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human GIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8060) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GIF at approximately 48-52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
GIF (Gastric Intrinsic Factor; also better known as IF or Intrinsic Factor) is a 45-55 kDa, secreted glycoprotein member of the eukaryotic cobalamin transport family of proteins. It should not be confused with either GIF/growth inhibitory factor/metallothionein 3, or GIF/glycosylation-inhibiting factor/macrophage migration inhibitory factor/phenylpyruvate tautomerase. GIF shows restricted expression, being essentially limited to parietal and select chief cells of the stomach. GIF binds to haptocorrin-associated cobalamin/VitB12 in the duodenum, whereupon it passes into the ileum and is taken up by a 500 kDa epithelial receptor named cubam, a complex of cubilin and amnionless molecules. Mature human GIF is 399 amino acids (aa) in length (aa 19-417) that contains one principal B12 binding site between aa 365-395. There is one isoform variant that shows a deletion of aa 3-27. Full-length human GIF shares 82% aa sequence identity with mouse GIF.
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