Human GITR/TNFRSF18 PE-conjugated Antibody

(7 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human GITR in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) 4‑1BB, recombinant mouse (rm) 4‑1BB, rhCD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFAS, rmGITR, rhHVEM, rhLymphotoxin R beta, rmLymphotoxin R beta, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII.
  • Source
    Monoclonal Mouse IgG1 Clone # 110416
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human GITR/TNFRSF18
    Gln26-Glu161 (Thr43Ala)
    Accession # Q9Y5U5
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of GITR/TNFRSF18 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 1 μg/mL PHA for 24 hours were stained with Mouse Anti-Human GITR/TNFRSF18 PE-conjugated Mono­clonal Antibody (Catalog # FAB689P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: GITR/TNFRSF18

GITR (glucocorticoid-induced tumor necrosis factor receptor, also named AITR, activation-inducible TNF R family member), is a 228 amino acid (aa) type I transmembrane protein belonging to the TNF R family and has been designated TNFRSF18. The GITR cytoplasmic domain has striking homology with the cytoplasmic domain of 4-1BB and CD27. Human GITR shares 55% homology with murine GITR. GITR is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen, and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is up‑regulated by antigen stimulation or by treatment with anti-CD3 plus anti-CD28, or PMA plus ionomycin. Human GITR ligand was identified from human umbilical vein endothelial cells and is a 177 aa polypeptide belonging to the TNF superfamily (TNFSF18). Ligation of GITR can activate NF-kappa B through TRAF2, and protect T cells from TCR activation-induced cell death. It has been proposed that GITR ligand and GITR may modulate T lymphocyte functions.

  • References:
    1. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
    2. Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
    3. Gurney, A.L. et al. (1999) Current Biology 9:215.
    4. Kwon, B. et al. (1999) Current Opinion in Immunology 11:340.
  • Long Name:
    Glucocorticoid Induced TNF Receptor Family Related Gene
  • Entrez Gene IDs:
    8784 (Human); 21936 (Mouse); 500598 (Rat)
  • Alternate Names:
    Activation-inducible TNFR family receptor; AITR; AITRTNF receptor superfamily activation-inducible protein; CD357 antigen; CD357; GITR-D; GITRtumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; TNFRSF18; tumor necrosis factor receptor superfamily, member 18
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 7 of 7
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Sample Type
  1. Systemic and local characterization of regulatory T cells in a chronic fungal infection in humans.
    Authors: Cavassani KA, Campanelli AP, Moreira AP, Vancim JO, Vitali LH, Mamede RC, Martinez R, Silva JS
    J. Immunol., 2006;177(9):5811-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow Cytometry,
  2. In vitro-expanded human CD4(+)CD25(+) T-regulatory cells can markedly inhibit allogeneic dendritic cell-stimulated MLR cultures.
    Authors: Godfrey WR, Ge YG, Spoden DJ, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2004;104(2):453-61.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow Cytometry,
  3. Functional regulatory T cells accumulate in aged hosts and promote chronic infectious disease reactivation.
    Authors: Lages CS, Suffia I, Velilla PA, Huang B, Warshaw G, Hildeman DA, Belkaid Y, Chougnet C
    J. Immunol., 2008;181(3):1835-48.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow Cytometry,
  4. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
    Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2005;105(2):750-8.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Flow Cytometry,
  5. Effects of the administration of high-dose interleukin-2 on immunoregulatory cell subsets in patients with advanced melanoma and renal cell cancer.
    Authors: van der Vliet HJ, Koon HB, Yue SC, Uzunparmak B, Seery V, Gavin MA, Rudensky AY, Atkins MB, Balk SP, Exley MA
    Clin. Cancer Res., 2007;13(7):2100-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow Cytometry,
  6. Antigen-induced regulatory T cells in HBV chronically infected patients.
    Authors: Barboza L, Salmen S, Goncalves L, Colmenares M, Peterson D, Montes H, Cartagirone R, Gutierrez Mdel C, Berrueta L
    Virology, 2007;368(1):41-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow Cytometry,
  7. Statins increase the frequency of circulating CD4+ FOXP3+ regulatory T cells in healthy individuals.
    Authors: Rodriguez-Perea A, Montoya C, Olek S, Chougnet C, Velilla P
    J Immunol Res, 2015;2015(0):762506.
    Species: Human
    Sample Type: Whole Blood
    Application: Flow Cytometry,
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 25
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 3 (Based on 1 review)

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We have 1 review tested in 1 application: Flow Cytometry.

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Summary

ApplicationFlow Cytometry
Sample TestedT regulatory cells
SpeciesHuman

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