Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 110416
View all GITR/TNFRSF18 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human GITR/TNFRSF18
Gln26-Glu161 (Thr43Ala)
Accession # Q9Y5U5

Specificity

Detects human GITR in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) 4‑1BB, recombinant mouse (rm) 4‑1BB, rhCD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFAS, rmGITR, rhHVEM, rhLymphotoxin R beta, rmLymphotoxin R beta, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human GITR/TNFRSF18 PE‑conjugated Antibody

Detection of GITR/TNFRSF18 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of GITR/TNFRSF18 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) treated with 1 µg/mL PHA for 24 hours were stained with Mouse Anti-Human GITR/TNFRSF18 PE-conjugated Mono-clonal Antibody (Catalog # FAB689P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of GITR/TNFRSF18 in RPMI8226 (positive cells) vs. Jurkat (negative cells) by Flow Cytometry

RPMI8226 cells (filled histogram) vs. Jurkat cells (open histogram) were stained with Mouse Anti-Human GITR/TNFRSF18 PE‑conjugated Monoclonal Antibody (Catalog # FAB689P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human GITR/TNFRSF18 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: - Human peripheral blood mononuclear cells (PBMCs) treated with PHA
- RPMI8226 cells

Reviewed Applications

Read 1 review rated 3 using FAB689P in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: GITR/TNFRSF18

GITR (glucocorticoid-induced tumor necrosis factor receptor, also named AITR, activation-inducible TNF R family member), is a 228 amino acid (aa) type I transmembrane protein belonging to the TNF R family and has been designated TNFRSF18. The GITR cytoplasmic domain has striking homology with the cytoplasmic domain of 4-1BB and CD27. Human GITR shares 55% homology with murine GITR. GITR is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen, and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is up‑regulated by antigen stimulation or by treatment with anti-CD3 plus anti-CD28, or PMA plus ionomycin. Human GITR ligand was identified from human umbilical vein endothelial cells and is a 177 aa polypeptide belonging to the TNF superfamily (TNFSF18). Ligation of GITR can activate NF-kappa B through TRAF2, and protect T cells from TCR activation-induced cell death. It has been proposed that GITR ligand and GITR may modulate T lymphocyte functions.

References

  1. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
  2. Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
  3. Gurney, A.L. et al. (1999) Current Biology 9:215.
  4. Kwon, B. et al. (1999) Current Opinion in Immunology 11:340.

Long Name

Glucocorticoid Induced TNF Receptor Family Related Gene

Alternate Names

AITR, CD357, TNFRSF18

Entrez Gene IDs

8784 (Human); 21936 (Mouse); 500598 (Rat); 102146362 (Cynomolgus Monkey)

Gene Symbol

TNFRSF18

UniProt

Q9Y5U5

Additional GITR/TNFRSF18 Products

Product Documents for Human GITR/TNFRSF18 PE‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human GITR/TNFRSF18 PE‑conjugated Antibody

For research use only

Citations for Human GITR/TNFRSF18 PE‑conjugated Antibody

Customer Reviews for Human GITR/TNFRSF18 PE‑conjugated Antibody (1)

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  • Human GITR/TNFRSF18 PE‑conjugated Antibody
    Name: SOjan Abraham
    Application: Flow Cytometry
    Sample Tested: T regulatory cells
    Species: Human
    Verified Customer | Posted 01/21/2016

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