Human Glutathione S‑Transferase mu 1/
GS Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Glutathione S‑Transferase mu 1/GS in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Glutathione S‑Transferase mu 1/GS
    Met1-Lys218
    Accession # P09488
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Glutathione S‑Transferase mu 1/
GS by Western Blot.
Western blot shows lysates of human kidney tissue, human adrenal gland tissue, human stomach tissue, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Glutathione S‑Transferase mu 1/GS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6894) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Glutathione S‑Transferase mu 1/GS at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glutathione S-Transferase mu 1/GSTM1
Glutathione S-Transferases (GSTs) are members of the phase II detoxification enzyme family that conjugate glutathione to various electrophilic compounds, including metabolites generated by oxidative processes in the body, environmental toxins or carcinogens, and anti-cancer drugs. GSTM1 is a cytosolic protein that belongs to the mu class of the GST superfamily. The gene encoding GSTM1 is mapped onto human chromosome 1p13.3 and is known to be highly polymorphic (1). Mostly notably, the widely occurring GSTM1-null genotype has been linked to a variety of cancers including lung (2), gastric (3), bladder (4) and prostate (5). In addition to its role in releasing oxidative stress, GSTM1 has also been suggested to act as a hormone binding protein and play a role in maintaining hormone homeostasis in the body (6, 7).
  • References:
    1. Pearson, W. R. et al. (1993) Am. J. Hum. Genet. 53:220.
    2. Mohr, L.C. et al. (2003) Anticancer. Res. 23:2111.
    3. Wang, H. et al. (2010) Dig. Dis. Sci. 55:1824.
    4. Engel, L. S. et al. (2002) Am. J. Epidermiol. 156:95.
    5. Mo, Z. et al. (2009) Prostate. 69:662.
    6. Mukherjee, S. B. et al. (1999) Biochem. J. 340:309.
    7. Ishigaki, S. et al. (1989) Arch. Biochem. Biophys. 273:265.
  • Entrez Gene IDs:
    2944 (Human)
  • Alternate Names:
    EC 2.5.1.18; glutathione S-alkyltransferase; glutathione S-aralkyltransferase; glutathione S-aryltransferase; glutathione S-transferase M1; Glutathione STransferase mu 1; Glutathione S-Transferase mu 1; GST class-mu 1; GST HB subunit 4; GST1; GSTM1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GTH4; GTM1; HB subunit 4; H-B; MGC26563; MU; MU-1; S-(hydroxyalkyl)glutathione lyase
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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