|Detection of Human Glycine N-methyltransferase/GNMT by Western Blot. Western blot shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human Glycine N-methyltransferase/GNMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6526) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Glycine N-methyltransferase/GNMT at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
GNMT (glycine N-methyltransferase) is a cytoplasmic homotetramer of 33 kDa units that converts S-adenosylmethionine to N-methylglycine (sarcosine), also producing S-adenosylhomocysteine. It is abundant in liver, but has also been found in the pancreas and prostate, and is downregulated in hepatocellular carcinoma. GNMT adjusts liver methyl group synthesis for dietary methionine, influencing DNA methylation. The 5-methyltetrahydrofolate form of folic acid binds and inhibits GNMT activity. The 295 amino acid (aa) human GNMT shares 92% aa identity with mouse and rat GNMT.
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