Human Glycine N-methyltransferase/GNMT Antibody

Catalog # Availability Size / Price Qty
Detection of Human Glycine N-methyltransferase/GNMT by Western Blot.
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Product Details
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Human Glycine N-methyltransferase/GNMT Antibody Summary

Species Reactivity
Detects human Glycine N-methyltransferase/GNMT in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human Glycine N-methyltransferase/GNMT
Accession # Q14749
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Glycine N-methyltransferase/GNMT antibody by Western Blot. View Larger

Detection of Human Glycine N-methyltransferase/GNMT by Western Blot. Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human Glycine N-methyltransferase/GNMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6526) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Glycine N-methyltransferase/GNMT at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glycine N-Methyltransferase/GNMT

GNMT (glycine N-methyltransferase) is a cytoplasmic homotetramer of 33 kDa units that converts S-adenosylmethionine to N-methylglycine (sarcosine), also producing S-adenosylhomocysteine. It is abundant in liver, but has also been found in the pancreas and prostate, and is downregulated in hepatocellular carcinoma. GNMT adjusts liver methyl group synthesis for dietary methionine, influencing DNA methylation. The 5-methyltetrahydrofolate form of folic acid binds and inhibits GNMT activity. The 295 amino acid (aa) human GNMT shares 92% aa identity with mouse and rat GNMT.

Entrez Gene IDs
27232 (Human); 14711 (Mouse); 25134 (Rat)
Alternate Names
EC; Glycine NMethyltransferase; Glycine N-Methyltransferase; GNMT

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