Human Glypican 1 Antibody AF4519: R&D Systems

Human Glypican 1 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Glypican 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant mouse Glypican 1 is observed and less than 1% cross-reactivity with recombinant human (rh) Glypican 5 and rhGlypican 6 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Glypican 1
    Asp24-Ser530
    Accession # P35052
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Glypican 1 (Catalog # 4519-GP)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Glypican 1 in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA‑MB‑231 human breast cancer cell line was stained with Goat Anti-Human Glypican 1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4519, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Immunocytochemistry
Glypican 1 in MDA‑MB‑231 Human Cell Line. Glypican 1 was detected in immersion fixed MDA‑MB‑231 human breast cancer cell line using Goat Anti-Human Glypican 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4519) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glypican 1

The Glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked proteoglycans that play a key role in growth factor signaling (1, 2, 3, 4). There are six known mammalian Glypicans. They all share a common-sized protein core of 60‑70 kDa, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C-terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of Glypicans are known, with one subfamily containing Glypicans 1, 2, 4 and 6, and another subfamily containing Glypicans 3 and 5 (3, 5). Human Glypican 1 (GPC-1) is synthesized as a 558 amino acid (aa) preproprecursor that contains a 23 aa signal sequence, a 507 aa mature segment, and a 28 aa C-terminal prosegment (6, 7). There are two potential N-linked and four potential O-linked sites for glycosylation or glycanation. There are potentially two heparan sulfate (HS) modifications on GPC-1 that could contribute to a native molecular weight of approximately 200 kDa (7, 8, 9). Mature human GPC-1 shares 91% aa identity with mature mouse GPC-1. There are two potential splice variants of human GPC-1. Both show an alternate start site at Met73, while one has an additional 65 aa substitution for the C-terminal 264 amino acids (10, 11). Cells known to express GPC-1 include neurons, smooth and skeletal muscle cells, keratinocytes, osteoblasts, Schwann cells, immature dendritic cells, and tumor, plus tumor-associated vascular endothelial cells (8, 9, 12‑15). The function of GPC-1 is complex and varied. As a proteoglycan, it appears to make use of its HS adduct to impact select growth factor activity (16). This is accomplished by having juxtramembrane HS attachment sites, and a flexible, GPI-linkage (17). Data suggests GPC-1 and sulfation enzymes may collaborate to regulate FGF signaling. HS modules that are rich in 2-O- and 6-O- sulfate upregulate FGF-2 activation of FGFR1c (18). Similarly, FGF-1 requires both 2-O- and 6-O-sulfation to bind to FGFR2c and 3c. By contract, FGF-1 requires no sulfation to bind to FGFR2b, and FGF-8b needs only 6-O-sulfation to activate FGFR3c. Thus, many FGF receptor isoform specific effects may be attributed to an interaction between Glypican family members and the cell sulfation system (19).

  • References:
    1. Song, H.H. and J. Filmus (2002) Biochim. Biophys. Acta 1573:241.
    2. Fransson, L-A. et al. (2004) Cell. Mol. Life Sci. 61:1016.
    3. De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117.
    4. Lamoureux, F. et al. (2007) BioEssays 29:758.
    5. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
    6. GenBank Accession # P35052.
    7. David, G. et al. (1990) J. Cell Biol. 111:3165.
    8. Lories, V. et al. (1992) J. Biol. Chem. 267:1116.
    9. Lories, V. et al. (1989) J. Biol. Chem. 264:7009.
    10. GenBank Accession # EAW71184.
    11. GenBank Accession # EAW71183.
    12. Chernousov, M.A. et al. (2006) J. Neurosci. 26:508.
    13. Wegrowski, Y. et al. (2006) Clin. Exp. Immunol. 144:485.
    14. Qiao, D. et al. (2003) J. Biol. Chem. 278:16045.
    15. Kayed, H. et al. (2006) Int. J. Oncol. 29:1139.
    16. Selleck, S.B. (2006) SciSTKE, April 4:pe17.
    17. Qiao, D. et al. (2003) J. Biol. Chem. 278:16045.
    18. Su, G. et al. (2006) Am. J. Pathol. 168:2014.
    19. Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637.
  • Entrez Gene IDs:
    2817 (Human); 14733 (Mouse); 58920 (Rat)
  • Alternate Names:
    FLJ38078; Glypican 1; glypican proteoglycan 1; glypican; glypican-1; GPC1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Overexpression of glypican-1 implicates poor prognosis and their chemoresistance in oesophageal squamous cell carcinoma
    Authors: Hisashi Hara
    Br J Cancer, 2016;115(1):66-75.
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