Human Glypican 1 Antibody

Catalog # Availability Size / Price Qty
AF4519
AF4519-SP
Detection of Glypican 1 in MDA‑MB‑231 Human Cell Line by Flow Cytometry.
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Human Glypican 1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Glypican 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant mouse Glypican 1 is observed and less than 1% cross-reactivity with recombinant human (rh) Glypican 5 and rhGlypican 6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Glypican 1
Asp24-Ser530
Accession # P35052
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Glypican 1 (Catalog # 4519-GP)
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Glypican 1 in MDA‑MB‑231 Human Cell Line by Flow Cytometry. View Larger

Detection of Glypican 1 in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA‑MB‑231 human breast cancer cell line was stained with Goat Anti-Human Glypican 1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4519, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Immunocytochemistry Glypican 1 in MDA‑MB‑231 Human Cell Line. View Larger

Glypican 1 in MDA‑MB‑231 Human Cell Line. Glypican 1 was detected in immersion fixed MDA‑MB‑231 human breast cancer cell line using Goat Anti-Human Glypican 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4519) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glypican 1

The Glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked proteoglycans that play a key role in growth factor signaling (1, 2, 3, 4). There are six known mammalian Glypicans. They all share a common-sized protein core of 60‑70 kDa, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C-terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of Glypicans are known, with one subfamily containing Glypicans 1, 2, 4 and 6, and another subfamily containing Glypicans 3 and 5 (3, 5). Human Glypican 1 (GPC-1) is synthesized as a 558 amino acid (aa) preproprecursor that contains a 23 aa signal sequence, a 507 aa mature segment, and a 28 aa C-terminal prosegment (6, 7). There are two potential N-linked and four potential O-linked sites for glycosylation or glycanation. There are potentially two heparan sulfate (HS) modifications on GPC-1 that could contribute to a native molecular weight of approximately 200 kDa (7, 8, 9). Mature human GPC-1 shares 91% aa identity with mature mouse GPC-1. There are two potential splice variants of human GPC-1. Both show an alternate start site at Met73, while one has an additional 65 aa substitution for the C-terminal 264 amino acids (10, 11). Cells known to express GPC-1 include neurons, smooth and skeletal muscle cells, keratinocytes, osteoblasts, Schwann cells, immature dendritic cells, and tumor, plus tumor-associated vascular endothelial cells (8, 9, 12‑15). The function of GPC-1 is complex and varied. As a proteoglycan, it appears to make use of its HS adduct to impact select growth factor activity (16). This is accomplished by having juxtramembrane HS attachment sites, and a flexible, GPI-linkage (17). Data suggests GPC-1 and sulfation enzymes may collaborate to regulate FGF signaling. HS modules that are rich in 2-O- and 6-O- sulfate upregulate FGF-2 activation of FGFR1c (18). Similarly, FGF-1 requires both 2-O- and 6-O-sulfation to bind to FGFR2c and 3c. By contract, FGF-1 requires no sulfation to bind to FGFR2b, and FGF-8b needs only 6-O-sulfation to activate FGFR3c. Thus, many FGF receptor isoform specific effects may be attributed to an interaction between Glypican family members and the cell sulfation system (19).

References
  1. Song, H.H. and J. Filmus (2002) Biochim. Biophys. Acta 1573:241.
  2. Fransson, L-A. et al. (2004) Cell. Mol. Life Sci. 61:1016.
  3. De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117.
  4. Lamoureux, F. et al. (2007) BioEssays 29:758.
  5. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
  6. GenBank Accession # P35052.
  7. David, G. et al. (1990) J. Cell Biol. 111:3165.
  8. Lories, V. et al. (1992) J. Biol. Chem. 267:1116.
  9. Lories, V. et al. (1989) J. Biol. Chem. 264:7009.
  10. GenBank Accession # EAW71184.
  11. GenBank Accession # EAW71183.
  12. Chernousov, M.A. et al. (2006) J. Neurosci. 26:508.
  13. Wegrowski, Y. et al. (2006) Clin. Exp. Immunol. 144:485.
  14. Qiao, D. et al. (2003) J. Biol. Chem. 278:16045.
  15. Kayed, H. et al. (2006) Int. J. Oncol. 29:1139.
  16. Selleck, S.B. (2006) SciSTKE, April 4:pe17.
  17. Qiao, D. et al. (2003) J. Biol. Chem. 278:16045.
  18. Su, G. et al. (2006) Am. J. Pathol. 168:2014.
  19. Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637.
Entrez Gene IDs
2817 (Human); 14733 (Mouse); 58920 (Rat)
Alternate Names
FLJ38078; Glypican 1; glypican proteoglycan 1; glypican; glypican-1; GPC1

Product Datasheets

Citation for Human Glypican 1 Antibody

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