GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) induces the development of monocytes, neutrophils, eosinophils, and myeloid and dermal dendritic cells. It also acts as a neutrophil and dendritic cell chemoattractant. GM-CSF promotes Th1 and Th17 cell mediated autoimmune inflammation as well as the inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it cooperates with G-CSF in promoting tumor cell proliferation and invasion. GM-CSF signals through a receptor complex composed of GM-CSF R alpha and the Common beta Chain (beta c) with Syndecan-2 as a potential co-receptor. The beta c subunit is shared by the receptor complexes for IL-3 and IL-5.
Human GM-CSF Quantikine HS ELISA Kit
R&D Systems | Catalog # HSGM0
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Key Product Details
Assay Length
6.5 hours
Sample Type & Volume Required Per Well
Serum (150 µL), EDTA Plasma (150 µL), Heparin Plasma (150 µL), Urine (200 µL)
Sensitivity
0.26 pg/mL
Assay Range
0.5-32 pg/mL (Urine)
1-64 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
1-64 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Human GM-CSF Quantikine HS ELISA Kit
The Quantikine HS Human GM-CSF Immunoassay is a 6.5 hour solid phase ELISA designed to measure human GM-CSF in serum, plasma, and urine. It contains E. coli-expressed recombinant human GM-CSF and antibodies raised against the recombinant factor. It has been shown to accurately quantitate the recombinant factor. Results obtained using natural GM-CSF showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human GM-CSF.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450 or 490nm (TMB or AP Substrate)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human GM-CSF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 18 | 18 | 18 |
| Mean (pg/mL) | 3.68 | 22.0 | 44.6 | 2.8 | 16.2 | 33.2 |
| Standard Deviation | 0.35 | 1.07 | 1.76 | 0.66 | 2.47 | 4.6 |
| CV% | 9.5 | 4.9 | 4.0 | 23.5 | 15.3 | 13.9 |
Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 2.69 | 7.05 | 24.8 | 3.77 | 14.3 | 27.4 |
| Standard Deviation | 0.14 | 0.33 | 0.73 | 0.37 | 1.17 | 1.85 |
| CV% | 5.2 | 4.7 | 2.9 | 9.8 | 8.2 | 6.8 |
Recovery for Human GM-CSF Quantikine HS ELISA Kit
The recovery of GM-CSF spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 114 | 88-140 |
| Heparin Plasma (n=4) | 109 | 91-122 |
| Serum (n=4) | 109 | 94-128 |
| Urine (n=5) | 102 | 95-116 |
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of GM-CSF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human GM-CSF Quantikine HS ELISA Kit
Human GM-CSF ELISA Urine Assay
Human GM-CSF ELISA Serum/Plasma Assay
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: GM-CSF
Long Name
Granulocyte Macrophage Growth Factor
Alternate Names
CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim
Entrez Gene IDs
Gene Symbol
CSF2
Additional GM-CSF Products
Product Documents for Human GM-CSF Quantikine HS ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human GM-CSF Quantikine HS ELISA Kit
For research use only
Related Research Areas
Citations for Human GM-CSF Quantikine HS ELISA Kit
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Protocols
View specific protocols for Human GM-CSF Quantikine HS ELISA Kit (HSGM0):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- For Serum & Plasma Samples: Add 100 µL of Assay Diluent to each well.
For Urine Samples: Add 50 µL of Assay Diluent to each well. - For Serum & Plasma Samples: Add 150 µL of Standard, control, or sample to each well.
For Urine Samples: Add 200 µL of Standard, control, or sample to each well. - Cover with a plate sealer, and incubate at room temperature for 3 hours.
- Aspirate each well and wash, repeating the process 5 times for a total of 6 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 6 times.
- Add 50 µL Substrate Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour. Do not wash the plate.
- Add 50 µL Amplifier Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.
- Add 50 µL of Stop Solution to each well. Read at 490 nm within 30 minutes. Set wavelength correction to 650 nm or 690 nm.
Assay Diluent
Standard, Control, or Sample
200 µL Conjugate
50 µL Substrate Solution
50 µL Amplifier Solution
50 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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