Human GPR101 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human GPR101 in Western blots.
  • Source
    Monoclonal Mouse IgG Clone # 909603
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human GPR101
    Met1-Pro508,
    Accession # NP_473362
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human GPR101 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line both non-transfected and transfected with human GPR101. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GPR101 Monoclonal Antibody (Catalog # MAB7495) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GPR101 at approximately 80-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of GPR101 in HEK293 Human Cell Line Transfected with Human GPR101 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GPR101 and eGFP was stained with either (A) Mouse Anti-Human GPR101 Monoclonal Antibody (Catalog # MAB7495) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GPR101
GPR101 (G-protein coupled receptor 101), is a member of a family of proteins that contain seven transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPR101 is an orphan G protein-coupled receptor of unknown function. mRNA for this gene is detected predominantly in the brain for both human and mouse. The expression of GPR101 is altered by pregnancy and lactation in the rat.
  • Long Name:
    G Protein-coupled Receptor 101
  • Entrez Gene IDs:
    83550 (Human); 245424 (Mouse); 317608 (Rat)
  • Alternate Names:
    G protein-coupled receptor 101; GPCR6; GPR101; probable G-protein coupled receptor 101
Related Research Areas
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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Mouse IgG Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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