Human GPR101 Antibody

Catalog # Availability Size / Price Qty
MAB7495
MAB7495-SP
Detection of Human GPR101 by Western Blot.
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Human GPR101 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GPR101 in Western blots.
Source
Monoclonal Mouse IgG Clone # 909603
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human GPR101
Met1-Pro508,
Accession # NP_473362
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human GPR101 by Western Blot. View Larger

Detection of Human GPR101 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line both non-transfected and transfected with human GPR101. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GPR101 Monoclonal Antibody (Catalog # MAB7495) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GPR101 at approximately 80-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of GPR101 in HEK293 Human Cell Line Transfected with Human GPR101 and eGFP by Flow Cytometry. View Larger

Detection of GPR101 in HEK293 Human Cell Line Transfected with Human GPR101 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GPR101 and eGFP was stained with either (A) Mouse Anti-Human GPR101 Monoclonal Antibody (Catalog # MAB7495) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR101

GPR101 (G-protein coupled receptor 101), is a member of a family of proteins that contain seven transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPR101 is an orphan G protein-coupled receptor of unknown function. mRNA for this gene is detected predominantly in the brain for both human and mouse. The expression of GPR101 is altered by pregnancy and lactation in the rat.

Long Name
G Protein-coupled Receptor 101
Entrez Gene IDs
83550 (Human); 245424 (Mouse); 317608 (Rat)
Alternate Names
G protein-coupled receptor 101; GPCR6; GPR101; probable G-protein coupled receptor 101

Product Datasheets

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Reconstitution Buffers

Secondary Antibodies

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