Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 909603
View all GPR101 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human GPR101
Met1-Pro508,
Accession # NP_473362

Specificity

Detects human GPR101 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for Human GPR101 Antibody

Detection of Human GPR101 antibody by Western Blot.

Detection of Human GPR101 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line both non-transfected and transfected with human GPR101. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GPR101 Monoclonal Antibody (Catalog # MAB7495) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GPR101 at approximately 80-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of GPR101 antibody in HEK293 Human Cell Line Transfected with Human GPR101 and eGFP antibody by Flow Cytometry.

Detection of GPR101 in HEK293 Human Cell Line Transfected with Human GPR101 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human GPR101 and eGFP was stained with either (A) Mouse Anti-Human GPR101 Monoclonal Antibody (Catalog # MAB7495) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human GPR101 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human GPR101 and eGFP

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human GPR101

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GPR101

GPR101 (G-protein coupled receptor 101), is a member of a family of proteins that contain seven transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPR101 is an orphan G protein-coupled receptor of unknown function. mRNA for this gene is detected predominantly in the brain for both human and mouse. The expression of GPR101 is altered by pregnancy and lactation in the rat.

Long Name

G Protein-coupled Receptor 101

Alternate Names

GPCR6

Entrez Gene IDs

83550 (Human); 245424 (Mouse); 317608 (Rat)

Gene Symbol

GPR101

UniProt

NP_473362

Additional GPR101 Products

Product Documents for Human GPR101 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GPR101 Antibody

For research use only

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