Human GPR101 Antibody Summary
Accession # NP_473362
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human GPR101 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line both non-transfected and transfected with human GPR101. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GPR101 Monoclonal Antibody (Catalog # MAB7495) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GPR101 at approximately 80-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of GPR101 in HEK293 Human Cell Line Transfected with Human GPR101 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GPR101 and eGFP was stained with either (A) Mouse Anti-Human GPR101 Monoclonal Antibody (Catalog # MAB7495) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
GPR101 (G-protein coupled receptor 101), is a member of a family of proteins that contain seven transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPR101 is an orphan G protein-coupled receptor of unknown function. mRNA for this gene is detected predominantly in the brain for both human and mouse. The expression of GPR101 is altered by pregnancy and lactation in the rat.
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