GPR111 (G-protein coupled receptor 111; also PRG20) is a 71 kDa (predicted), seven transmembrane (TM) member of the GPR-2 family, LN-7TM subfamily of molecules. It is reportedly expressed in lung, mammary gland and diencephalon. Human GPR111 is 642 amino acids (aa) in length. It contains an extended N-terminal extracellular region with a mucin like stalk (aa 1-383), followed by a series of seven TM domains and a short C-terminal cytoplasmic tail. The N-terminus possesses a GPS (GPCR proteolytic site) (aa 324-368) that likely generates a soluble cleavage product. GPR111 is considered an adhesion-type GPCR, and as such, is expected to form dimers, if not oligomers. There is one potential splice variant for GPR111. It shows a 92 aa substitution for aa 1-24 coupled to a 19 aa substitution for aa 622-642. Over aa 19-375, human GPR111 shares 68% aa identity with mouse GPR111.
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Cys19-Ser375
Accession # NP_722581
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human GPR111 Antibody
Detection of Human GPR111 by Western Blot.
Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 1 µg/mL of Human GPR111 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6494) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GPR111 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Applications for Human GPR111 Antibody
Western Blot
Sample: Human lung tissue
Formulation, Preparation, and Storage
Purification
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: GPR111
Long Name
Alternate Names
Gene Symbol
UniProt
Additional GPR111 Products
Product Documents for Human GPR111 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human GPR111 Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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