Human GRAIL/RNF128 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human GRAIL/RNF128 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human GRAIL/RNF128
    Val42-Pro161
    Accession # Q8TEB7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human GRAIL/RNF128 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human GRAIL/RNF128 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6234) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GRAIL/RNF128 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GRAIL/RNF128

GRAIL (Gene related to anergy in lymphocytes protein; also RNF128) is a 45-47 kDa, type I transmembrane E3 ubiquitin ligase. It is expressed in the endosomes of select cell types such as early myeloid progenitors and lymphocytes, and mediates the ubiquitination of membrane proteins such as CD3, CD40L, and CD83, plus the tetraspanins CD81 and CD151. GRAIL apparently regulates the threshold for T cell activation, and as such, plays a key role in autoimmunity. In Treg cells, GRAIL promotes suppressive activity by restricting Th17 gene expression. Mature human GRAIL is 390 amino acids (aa) in length (aa 39-428). It possesses a 169 aa luminal region (aa 39-207) and a 190 aa cytoplasmic domain. The luminal region contains one PA (protease-associated) domain that mediates protein-protein interactions (aa 48-193), and the cytoplasmic segment shows one zinc-finger region (aa 277-318). There is one splice variant that shows a 134 aa substitution for aa 1-161. Over aa 42-161, human GRAIL shares 98% aa identity with mouse GRAIL.

  • Long Name:
    Gene Related to Anergy in Lymphocytes Protein/Ring Finger Protein 128
  • Entrez Gene IDs:
    79589 (Human); 66889 (Mouse); 315911 (Rat)
  • Alternate Names:
    EC 6.3.2; EC 6.3.2.-; FLJ23516; Gene related to anergy in lymphocytes protein; GRAIL; ring finger protein 128GRAILE3 ubiquitin-protein ligase RNF128; RNF128
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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