Granzyme A is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is the most abundant protease in CTL and NK cells. It induces caspase‑independent cell death when introduced into target cells by perforin (1). Human granzyme A is synthesized as a 262 amino acid (aa) precursor with a signal peptide (aa 1‑26), a propeptide (aa 27‑28) and a mature chain (aa 29‑262) (2). After being activated by lysyl endopeptidase removal of the propeptide, Granzyme A cleaves thioester substrates. Mature human granzyme A shares 71% amino acid sequence identity with mouse granzyme A.
Human Granzyme A Alexa Fluor™ Plus 647‑conjugated Antibody
R&D Systems | Catalog # AF2905AFP647
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry
Label
Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Specificity
Detects human Granzyme A in direct ELISAs.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Granzyme A Alexa Fluor™ Plus 647‑conjugated Antibody
Application
Recommended Usage
ELISA
Optimal dilution of this antibody should be experimentally determined.
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Granzyme A
References
- Lieberman, J. and Z. Fan (2003) Curr. Opin. Immunol. 15:553.
- Gershenfeld, H.K. et al. (1988) Proc. Natl. Acad. Sci. USA 85:1184.
Alternate Names
CTL Tryptase, CTLA3, Fragmentin-1, GZMA, HF, HFSP
Gene Symbol
GZMA
UniProt
Additional Granzyme A Products
Product Documents for Human Granzyme A Alexa Fluor™ Plus 647‑conjugated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Granzyme A Alexa Fluor™ Plus 647‑conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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