Human Granzyme B Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC2906G
Detection of Granzyme B in NK‑92 Human Cell Line by Flow Cytometry.
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Human Granzyme B Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Granzyme B in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) Granzyme A, rhGranzyme H, recombinant mouse (rm) Granzyme B, rmGranzyme C, rmGranzyme D, or rmGranzyme G.
Source
Monoclonal Mouse IgG2A Clone # 351927
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Granzyme B
Gly19-Tyr247
Accession # P10144
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of Granzyme B in NK‑92 Human Cell Line by Flow Cytometry. View Larger

Detection of Granzyme B in NK‑92 Human Cell Line by Flow Cytometry. NK‑92 human natural killer lymphoma cell line was stained with Mouse Anti-Human Granzyme B Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC2906G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Granzyme B

Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Human Granzyme B is synthesized as a precursor (247 residues) with a signal peptide (residues 1-18), a pro peptide (residues 19-20), and a mature chain (residues 21-247) (4-6). The recombinant human (rh) Granzyme B consisting of residues 19-247 was expressed and purified. After being activated by active cathepsin C, rhGranzyme B cleaves a thioester substrate described previously (3).

References
  1. Kam, C-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
  2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
  3. Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al., eds., pp. 1549 - 1552.
  4. Schmid, J. and C. Weissman (1987) J. Immunol. 139:250.
  5. Caputo, A. et al. (1988) J. Biol. Chem. 263:6363.
  6. Trapani, J.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6924.
Entrez Gene IDs
3002 (Human); 14939 (Mouse)
Alternate Names
C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; CTLA1; CTLA-1; CTSGL1; Fragmentin-2; Granzyme B; Granzyme-2; GRB; GrzB; GZMB; HLP; SECT

Product Datasheets

Citation for Human Granzyme B Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The IL-7R alpha pathway is quantitatively and functionally altered in CD8 T cells in multiple sclerosis.
    Authors: Kreft KL, Verbraak E, Wierenga-Wolf AF, van Meurs M, Oostra BA, Laman JD, Hintzen RQ
    J. Immunol., 2012;188(4):1874-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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