Human Growth Hormone Quantikine ELISA Kit

(3 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    7.18 pg/mL
  • Assay Range
    25.0 - 1,600 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human Growth Hormone
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
Product Summary
The Quantikine Human Growth Hormone Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Growth Hormone in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Growth Hormone and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Growth Hormone showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Growth Hormone.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Standard Deviation9.418.82618.245.663.6

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation813.829.820.249.582.2


The recovery of Growth Hormone spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 97-105
EDTA Plasma (n=4) 108 101-117
Heparin Plasma (n=4) 107 99-117
Serum (n=4) 105 98-111
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Growth Hormone were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Growth Hormone Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Growth Hormone
Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretory granules.
    • Entrez Gene IDs
      2688 (Human);
    • Alternate Names
      GH1; GHIGHD1B; GHNGrowth hormone; GH-Nsomatotropin; growth hormone 1Pituitary growth hormone; hGH-N; Somatotropin;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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    Sample Type
    1. Endocrine responses during overnight recovery from exercise: impact of nutrition and relationships with muscle protein synthesis.
      Authors: Betts JA, Beelen M, Stokes KA, Saris W HM, van Loon L JC
      Int J Sport Nutr Exerc Metab, 2011;21(5):398-409.
      Species: Human
      Sample Type: Plasma
    2. Skeletal muscle myofibrillar and sarcoplasmic protein synthesis rates are affected differently by altitude-induced hypoxia in native lowlanders.
      Authors: Holm L, Haslund ML, Robach P, van Hall G, Calbet JA, Saltin B, Lundby C
      PLoS ONE, 2010;5(12):e15606.
      Species: Human
      Sample Type: Plasma
    3. CYP2C19 pharmacogenetics in advanced cancer: compromised function independent of genotype.
      Authors: Helsby NA, Lo WY, Sharples K, Riley G, Murray M, Spells K, Dzhelai M, Simpson A, Findlay M
      Br. J. Cancer, 2008;99(8):1251-5.
      Species: Human
      Sample Type: Serum
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 736 Human Growth Hormone

    Ctrl QC99
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine Wash Buffer 1

    ELISA WA126 5
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