Human Growth Hormone Quantikine ELISA Kit

  (3 citations)
(1 Review)
    
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Product Details
Assay Procedure
Citations (3)
FAQs
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    7.18 pg/mL
  • Assay Range
    25.0 - 1,600 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human Growth Hormone
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC99 , Quantikine Immunoassay Control Set 736 Human Growth Hormone - Please Inquire
Product Summary
The Quantikine Human Growth Hormone Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Growth Hormone in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Growth Hormone and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Growth Hormone showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Growth Hormone.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 201 595 1091 209 623 1152
Standard Deviation 9.4 18.8 26 18.2 45.6 63.6
CV% 4.7 3.2 2.4 8.7 7.3 5.5

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 197 586 1092 215 639 1189
Standard Deviation 8 13.8 29.8 20.2 49.5 82.2
CV% 4.1 2.4 2.7 9.4 7.7 6.9

Recovery

The recovery of Growth Hormone spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 97-105
EDTA Plasma (n=4) 108 101-117
Heparin Plasma (n=4) 107 99-117
Serum (n=4) 105 98-111
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Growth Hormone were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 Growth Hormone [HRP]
Product Datasheets

Certificate of Analysis Lookup

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Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

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Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Growth Hormone
Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretory granules.
  • Entrez Gene IDs:
    2688 (Human)
  • Alternate Names:
    GH1; GHIGHD1B; GHNGrowth hormone; GH-Nsomatotropin; growth hormone 1Pituitary growth hormone; Growth Hormone; hGH-N; Somatotropin
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Sample Type
  1. Endocrine responses during overnight recovery from exercise: impact of nutrition and relationships with muscle protein synthesis.
    Authors: Betts JA, Beelen M, Stokes KA, Saris W HM, van Loon L JC
    Int J Sport Nutr Exerc Metab, 2011;21(5):398-409.
    Species: Human
    Sample Type: Plasma
  2. Skeletal muscle myofibrillar and sarcoplasmic protein synthesis rates are affected differently by altitude-induced hypoxia in native lowlanders.
    Authors: Holm L, Haslund ML, Robach P, van Hall G, Calbet JA, Saltin B, Lundby C
    PLoS ONE, 2010;5(12):e15606.
    Species: Human
    Sample Type: Plasma
  3. CYP2C19 pharmacogenetics in advanced cancer: compromised function independent of genotype.
    Authors: Helsby NA, Lo WY, Sharples K, Riley G, Murray M, Spells K, Dzhelai M, Simpson A, Findlay M
    Br. J. Cancer, 2008;99(8):1251-5.
    Species: Human
    Sample Type: Serum

FAQs

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ELISA Controls
Description Application Cat# Citations Images  

Quantikine Immunoassay Control Set 736 Human Growth Hormone

Ctrl QC99
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Supplemental ELISA Products
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Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
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Average Rating: 5 (Based on 1 review)

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