Human Growth Hormone Quantikine ELISA Kit

R&D Systems | Catalog # DGH00

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)

Sensitivity

7.18 pg/mL

Assay Range

25-1600 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
View all Growth Hormone ELISA Kits »

Product Summary for Human Growth Hormone Quantikine ELISA Kit

The Quantikine Human Growth Hormone Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Growth Hormone in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Growth Hormone and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Growth Hormone showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Growth Hormone.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human Growth Hormone

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Sample Values

Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence of human Growth Hormone in this assay. No medical histories were available for the donors used in this study.

FemalesMean of Detectable (pg/mL)% DetectableRange (pg/mL)
Serum (n=18)55010029.3-4360
EDTA plasma (n=18)64810030.2-5936
Heparin plasma (n=18)64110038.1-5392

MalesMean of Detectable (pg/mL)% DetectableRange (pg/mL)
Serum (n=17)62.083ND-120.5
EDTA plasma (n=17)56.486ND-99.2
Heparin plasma (n=17)58.186ND-109.6
ND=Non-detectable

Cell culture Supernates - - Human peripheral blood cells (1 x 106 cells/mL) were cultured in DMEM supplemented with 5% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernates were removed and assayed for levels of human Growth Hormone. No detectable levels were observed.




Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 201 595 1091 209 623 1152
Standard Deviation 9.4 18.8 26.0 18.2 45.6 63.6
CV% 4.7 3.2 2.4 8.7 7.3 5.5

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 197 586 1092 215 639 1189
Standard Deviation 8.0 13.8 29.8 20.2 49.5 82.2
CV% 4.1 2.4 2.7 9.4 7.7 6.9

Recovery for Human Growth Hormone Quantikine ELISA Kit

The recovery of Growth Hormone spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 97-105
EDTA Plasma (n=4) 108 101-117
Heparin Plasma (n=4) 107 99-117
Serum (n=4) 105 98-111

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Growth Hormone were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Growth Hormone ELISA Linearity

Scientific Data Images for Human Growth Hormone Quantikine ELISA Kit

Human Growth Hormone ELISA Calibrator Diluent RD5K Standard Curve

Human Growth Hormone ELISA Calibrator Diluent RD5K Standard Curve

Human Growth Hormone ELISA Calibrator Diluent RD6-15 Standard Curve

Human Growth Hormone ELISA Calibrator Diluent RD6-15 Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Growth Hormone

GH (Growth Hormone; Somatotropin) is a polypeptide hormone that regulates somatic growth and metabolism through interactions with Growth Hormone R/GHR. GH is released from the anterior pituitary in response to GH-releasing Hormone (GHRH) and Ghrelin, while its secretion is suppressed by Somatostatin. GH levels may also be regulated by Thyrotropin-releasing Hormone, Leptin, Galanin, Neuropeptide Y, and Cortisol. GH can circulate in complex with a shed soluble form of GHR known as GHBP. In addition to promoting bone and muscle mass, GH exerts immunomodulatory effects and induces lipolysis, lactation, amino acid uptake, and protein synthesis. Overproduction of GH can lead to acromegaly, a disorder characterized by enlargement of the hands, feet, and facial features, as well as enhanced risk of cardiovascular disease, respiratory disease, and malignancy. GH deficiency in adults may lead to increases in cardiovascular risk, body fat, and insulin sensitivity, as well as decreased muscle mass and bone density.

Alternate Names

GH-N, GH1, GHB5, GHN, IGHD1A, IGHD2, Somatotropin

Entrez Gene IDs

2688 (Human)

Gene Symbol

GH1

Additional Growth Hormone Products

Product Documents for Human Growth Hormone Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Growth Hormone Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Growth Hormone Quantikine ELISA Kit

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  • Human Growth Hormone Quantikine ELISA Kit
    Name: Milton Quintanilla
    Sample Tested: CART
    Verified Customer | Posted 10/07/2020
  • Human Growth Hormone Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 11/17/2018
    Human Growth Hormone Quantikine ELISA Kit DGH00
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    Name: Balaji Mahender
    Sample Tested: Urine
    Verified Customer | Posted 01/09/2018

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Protocols

View specific protocols for Human Growth Hormone Quantikine ELISA Kit (DGH00):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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