Human Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human HS3ST1 by Western Blot.
1 Image
Product Details
Supplemental Products

Human Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody Summary

Species Reactivity
Detects human HS3ST1 in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
Chinese hamster ovary cell line CHO-derived recombinant human HS3ST1
Accession # O14792
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human HS3ST1 (Catalog # 5968-ST), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human HS3ST1 antibody by Western Blot. View Larger

Detection of Human HS3ST1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Human HS3ST1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5968) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HS3ST1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1

Heparan sulfate is a highly sulfated polysaccharide that can be found on cell surface and within extracellular matrix. It is typically covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, can be considered as a highly sulfated version of heparan sulfate that is detached from the protein core and is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues can be sulfated at 2-O position by heparan sulfate 2-O sulfotransferase (HS2ST). The N‑-acetylglucosamine residues can be sulfated at N, 3-O, and 6-O positions by N-deacetylase/N-sulfotransferases (NDSTs), heparan sulfate 3-O sulfotransferases (HS3STs) and heparan sulfate 6-O sulfotransferases (HS6STs) respectively. There are seven HS3STs in the human genome (3, 4). HS3ST1 is a rate-limiting enzyme for generating an antithrombin-binding pentasaccharide epitope on heparan sulfate and heparin (5, 6). Unlike other sulfotransferases that have signal-anchor domains and are type II membrane integral proteins in Golgi apparatus, HS3ST1 lacks a transmembrane domain and is likely to be an intraluminal enzyme (7, 8).

  1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
  2. Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem. 71:435.
  3. Shworak, N.W. et al. (1999) J. Biol. Chem. 274:5170.
  4. Xu, D. et al. (2005) Biochem. J. 386:451.
  5. Rosenberg, R.D. et al. (1997) J. Clin. Invest. 100:S67.
  6. Esko, J.D and Lindahl, U. (2001) J. Clin. Invest. 108:169.
  7. Shworak, N.W. et al. (1997) J. Biol. Chem. 272:28008.
  8. Liu, J. et al. (1999) J. Biol. Chem. 274:5158.
Entrez Gene IDs
9957 (Human); 15476 (Mouse); 84406 (Rat)
Alternate Names
3OST; 3OST1; 3-OST-1; 3OST1Heparan sulfate 3-O-sulfotransferase 1; EC 2.8.2; EC; h3-OST-1; heparan sulfate (glucosamine) 3-O-sulfotransferase 1; Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1; heparan sulfate glucosamine 3-O-sulfotransferase 1; heparin-glucosamine 3-O-sulfotransferase; HS3ST1

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody

There are currently no reviews for this product. Be the first to review Human Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody and earn rewards!

Have you used Human Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review