Human Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody

Catalog # Availability Size / Price Qty

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

Detection of Human HS6ST1 by Western Blot.
1 Image
Product Details
Citations (1)
Supplemental Products

Human Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody Summary

Species Reactivity
Detects human HS6ST1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse HS6ST3 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
CHO cell-derived recombinant human HS6ST1
Accession # AAY14736
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human HS6ST1 antibody by Western Blot. View Larger

Detection of Human HS6ST1 by Western Blot. Western blot shows lysates of H4 human neuroglioma cell line. PVDF Membrane was probed with 1 µg/mL of Human HS6ST1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5057) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HS6ST1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1

Heparan Sulfate is a highly sulfated polysaccharide that can be found on cell surface and extracellular matrix. It is usually covalently attached to a protein core as the glycan component of a proteoglycan. Heparan Sulfate interacts with a variety of proteins, such as growth factors, protease inhibitors, cytokines, lipoprotein lipase and viral envelope proteins, thus plays roles from cell growth, cell differentiation, cell motility, blood coagulation, lipid metabolism to viral infection (1, 2). Heparan Sulfate consists of repeating residues of uronic acid and N‑acetylglucosamine. The uronic acid residues can be sulfated at 2-O position by Heparan Sulfate 2-O Sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be sulfated at N-, 3-O, and 6-O positions by N‑deacetylase/N‑sulfotransferases (NDSTs), Heparan Sulfate 3-O and 6-O sulfotransferases (HS3STs and HS6STs) respectively. However, the reactions catalyzed by these sulfotransferases are normally incomplete on the whole chain of Heparan Sulfate. As a result, Heparan Sulfate displays enormous sequence diversity that allows it to interact with a wide spectrum of proteins differently. Among three HS6STs, HS6ST1 is the first to be cloned (3). Mice deficient of the HS6ST1 homologue gene showed embryonic lethality (4).

  1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
  2. Esko, J.D. and S.B. Selleck (2002) Annu. Rev. Biochem. 71:435.
  3. Habuchi, H et al. (1998) J. Biol. Chem. 273:9208.
  4. Habuchi, H et al. (2007) J. Biol. Chem. 282:15578.
  5. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
  6. MacRae, I.J. et al. (2000) Biochemistry 39:1613.
  7. Wu, Z.L. et al. (2002) Faseb J. 16:539.
Entrez Gene IDs
9394 (Human); 50785 (Mouse); 316325 (Rat)
Alternate Names
DKFZp547H098; EC 2.8.2; EC 2.8.2.-; FLJ25392; heparan sulfate 6-O-sulfotransferase 1; heparan-sulfate 6-sulfotransferase; HS6ST1; HS6ST-1; HS6STheparan-sulfate 6-O-sulfotransferase 1; MGC116899; MGC116901

Product Datasheets

You must select a language.


Citation for Human Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Multiplex genome editing of mammalian cells for producing recombinant heparin
    Authors: BE Thacker, KJ Thorne, C Cartwright, J Park, K Glass, A Chea, BP Kellman, NE Lewis, Z Wang, A Di Nardo, ST Sharfstein, W Jeske, J Walenga, J Hogwood, E Gray, B Mulloy, JD Esko, CA Glass
    Metabolic engineering, 2022-01-14;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody

There are currently no reviews for this product. Be the first to review Human Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody and earn rewards!

Have you used Human Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review