Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Hepsin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Hepsin
Ser46-Leu417 with Asp161Glu and Arg162Lys substitutions
Accession # P05981

Specificity

Detects human Hepsin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Hepsin Antibody

Detection of Human Hepsin antibody by Western Blot.

Detection of Human Hepsin by Western Blot.

Western blot shows lysates of human prostate tissue, human liver tissue, LNCaP human prostate cancer cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Hepsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4776) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Hepsin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.

Applications for Human Hepsin Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human prostate tissue, human liver tissue, LNCaP human prostate cancer cell line, and PC‑3 human prostate cancer cell line

Reviewed Applications

Read 1 review rated 5 using AF4776 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Hepsin

Hepsin, also known as TMPRSS1, is a Type II membrane protein with an extracellular serine protease domain (1). It is most highly expressed in liver, but is also present in many other tissues, notably lung, kidney, and skeletal muscle (2). A soluble form of Hepsin lacking the transmembrane domain has been identified (3). Hepsin is capable of activating Factor VII, and may initiate blood coagulation at the cell surface (4). Hepsin is overexpressed in various human tumors, including prostate (5), and is considered to be a biomarker for prostate cancer (6).

References

  1. Leytus, S.P. et al. (1988) Biochemistry 27:1067.
  2. Tsuji, A. et al. (1991) J. Biol. Chem. 266:16948.
  3. Li, Y. et al. (2005) Biomed. Biochim. Acta 1681:157.
  4. Kazama, Y. et al. (1995) J. Biol. Chem. 270:66.
  5. Dhanasekaran, S.M. et al. (2001) Nature 412:822.
  6. Wu, Q. and Parry, G. (2007) Front. Biosci. 12:5052.

Alternate Names

HPN, TMPRSS1

Entrez Gene IDs

3249 (Human); 15451 (Mouse); 29135 (Rat)

Gene Symbol

HPN

UniProt

P05981

Additional Hepsin Products

Product Documents for Human Hepsin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Hepsin Antibody

For research use only

Citations for Human Hepsin Antibody

Customer Reviews for Human Hepsin Antibody (1)

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  • Human Hepsin Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 05/22/2019
    AF4776 was used as the detection antibody in a sandwich ELISA with MAB4776 as the capture antibody.
    Human Hepsin Antibody AF4776

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