Human HGF Antibody

(17 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human HGF in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse HGF is observed. In Western blots, no cross-reactivity with recombinant canine HGF is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HGF
    Accession # P14210
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize HGF-induced IL-11 secretion in the Saos‑2 human osteosarcoma cell line. Hjertner, O. et al. (1999) Blood 94:3883.The Neutralization Dose (ND50) is typically 0.01-0.06 μg/mL in the presence of 3 ng/mL Recombinant Human HGF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Recombinant Human HGF by Western Blot. Western blot shows 25 ng of Recombinant Human HGF (Catalog #
294‑HG), Recombinant Mouse HGF (Catalog # 2207-HG) and Recombinant Canine HGF (Catalog # 3386-HG). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-294-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for HGF at approximately 30 and 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Immunohistochemistry
HGF in Human Liver.

HGF was detected in formalin fixed paraffin-embedded sections of human liver using 15 µg/mL Goat Anti-Human HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑294‑NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

IL-11 Secretion Induced by HGF and Neutralization by Human HGF Antibody.

Recombinant Human HGF induces IL-11 secretion in the Saos‑2 human osteosarcoma cell line. The dose-dependent response (orange line) is measured by the Human IL-11 Duoset (Catalog # DY218). Under these conditions, IL-11 secretion elicited by HGF is neutralized (green line) by increasing concentrations of Goat Anti-Human HGF Antigen Affinity-purified Polyclonal Antibod (Catalog #
AF-294-NA). The ND50 is typically 0.01‑0.06 μg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HGF

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity. Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase-like domain and different numbers of the Kringle domains. Human HGF shares 91%‑94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF-dependent c-MET activation is implicated in the development of many human cancers. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. HGF induces the upregulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription. This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis. HGF can also alter epithelium morphology by the induction of nectin-1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions. In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake. HGF promotes the motility of cardiac stem cells in damaged myocardium.

  • Long Name:
    Hepatocyte Growth Factor
  • Entrez Gene IDs:
    3082 (Human); 15234 (Mouse); 403441 (Canine)
  • Alternate Names:
    deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Matriptase regulates c-Met mediated proliferation and invasion in inflammatory breast cancer
    Authors: GL Zoratti, LM Tanabe, TE Hyland, MJ Duhaime, É Colombo, R Leduc, E Marsault, MD Johnson, CY Lin, J Boerner, JE Lang, K List
    Oncotarget, 2016;7(36):58162-58173.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling.
    Authors: Zoratti G, Tanabe L, Varela F, Murray A, Bergum C, Colombo E, Lang J, Molinolo A, Leduc R, Marsault E, Boerner J, List K
    Nat Commun, 2015;6(0):6776.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  3. Activated platelets interfere with recruitment of mesenchymal stem cells to apoptotic cardiac cells via high mobility group box 1/Toll-like receptor 4-mediated down-regulation of hepatocyte growth factor receptor MET.
    Authors: Vogel S, Chatterjee M, Metzger K, Borst O, Geisler T, Seizer P, Muller I, Mack A, Schumann S, Buhring H, Lang F, Sorg R, Langer H, Gawaz M
    J Biol Chem, 2014;289(16):11068-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Functional mononucleotide repeat polymorphism in the promoter region of HGF is associated with risk and malignant aggressiveness of bladder cancer.
    Authors: Chiba S, Tsuchiya N, Horikawa Y, Narita S, Inoue T, Akihama S, Saito M, Numakura K, Tsuruta H, Huang M, Satoh S, Habuchi T
    Int J Oncol, 2014;44(3):678-84.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - paraffin
  5. Spontaneous genomic alterations in a chimeric model of colorectal cancer enable metastasis and guide effective combinatorial therapy.
    Authors: Zhou Y, Rideout W, Bressel A, Yalavarthi S, Zi T, Potz D, Farlow S, Brodeur J, Monti A, Reddipalli S, Xiao Q, Bottega S, Feng B, Chiu M, Bosenberg M, Heyer J
    PLoS ONE, 2014;9(8):e105886.
  6. Combined targeting of mTOR and c-MET signaling pathways for effective management of epithelioid sarcoma.
    Authors: Imura, Yoshinor, Yasui, Hirohiko, Outani, Hidetats, Wakamatsu, Toru, Hamada, Kenichir, Nakai, Takaaki, Yamada, Shutaro, Myoui, Akira, Araki, Nobuhito, Ueda, Takafumi, Itoh, Kazuyuki, Yoshikawa, Hideki, Naka, Norifumi
    Mol Cancer, 2014;13(0):185.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  7. Associations between hepatocyte growth factor, c-Met, and basic fibroblast growth factor and survival in endometrial cancer patients.
    Authors: Felix AS, Edwards RP, Stone RA
    Br. J. Cancer, 2012;106(12):2004-9.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. Heparanase Plays a Dual Role in Driving Hepatocyte Growth Factor (HGF) Signaling by Enhancing HGF Expression and Activity.
    Authors: Ramani VC, Yang Y, Ren Y
    J. Biol. Chem., 2011;286(8):6490-9.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: IP
  9. c-Met-induced epithelial carcinogenesis is initiated by the serine protease matriptase.
    Authors: Szabo R, Rasmussen AL, Moyer AB
    Oncogene, 2011;30(17):2003-16.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  10. Wnt activity defines colon cancer stem cells and is regulated by the microenvironment.
    Authors: Vermeulen L, De Sousa E Melo F, van der Heijden M, Cameron K, de Jong JH, Borovski T, Tuynman JB, Todaro M, Merz C, Rodermond H, Sprick MR, Kemper K, Richel DJ, Stassi G, Medema JP
    Nat. Cell Biol., 2010;12(5):468-76.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  11. Alternative proteolytic processing of hepatocyte growth factor during wound repair.
    Authors: Buchstein N, Hoffmann D, Smola H, Lang S, Paulsson M, Niemann C, Krieg T, Eming SA
    Am. J. Pathol., 2009;174(6):2116-28.
    Species: Human
    Sample Type: Tissue Secretion
    Application: WB
  12. Endogenous hepatocyte growth factor is a niche signal for subventricular zone neural stem cell amplification and self-renewal.
    Authors: Nicoleau C, Benzakour O, Agasse F, Thiriet N, Petit J, Prestoz L, Roger M, Jaber M, Coronas V
    Stem Cells, 2008;0(0):.
    Species: Canine
    Sample Type: Whole Cells
    Application: Neut
  13. IFATS Series: FGF-2-induced HGF Secretion By Adipose-Derived Stromal Cells Inhibits Post-Injury Fibrogenesis Through A JNK-Dependent Mechanism.
    Authors: Suga H, Eto H, Shigeura T, Inoue K, Aoi N, Kato H, Nishimura S, Manabe I, Gonda K, Yoshimura K
    Stem Cells, 2008;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  14. Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
    Authors: Skibinski G, Elborn JS, Ennis M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L69-76.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  15. Hepatocyte growth factor acts as a motogen and guidance signal for gonadotropin hormone-releasing hormone-1 neuronal migration.
    Authors: Giacobini P, Messina A, Wray S, Giampietro C, Crepaldi T, Carmeliet P, Fasolo A
    J. Neurosci., 2007;27(2):431-45.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  16. Hepatocyte growth factor in polymorphonuclear leukocytes is increased in patients with systemic inflammatory response syndrome.
    Authors: Matsushima A, Ogura H, Koh T, Fujita K, Yoshiya K, Sumi Y, Hosotsubo H, Kuwagata Y, Tanaka H, Shimazu T, Sugimoto H
    J Trauma, 2004;56(2):259-64.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  17. Establishment and characterization of a biphasic synovial sarcoma cell line, SYO-1.
    Authors: Kawai A, Naito N, Ouchida M, Beppu Y
    Cancer Lett., 2004;204(1):105-13.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 17 Citations
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