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Recombinant Human HGF Protein

R&D Systems | Catalog # 294-HG

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Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Human HGF Protein (294-HG)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

P14210.2

Structure / Form

Disulfide-linked heterodimer

Applications

Bioactivity
View all HGF Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human HGF protein
Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

alpha chain: No results obtained; Gln32 predicted
beta chain: Val495

Predicted Molecular Mass

53.7 kDa ( alpha chain), 26 kDa ( beta chain)

SDS-PAGE

60-70 kDa, non-reducing conditions
60 kDa, and 33-34 kDa, reducing conditions

Activity

Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883.
The ED50 for this effect is equal to or less than 4.00 ng/mL.

Reviewed Applications

Read 6 reviews rated 4.8 using 294-HG in the following applications:

Scientific Data Images for Recombinant Human HGF Protein

Recombinant Human HGF Protein Bioactivity

Recombinant Human HGF Protein Bioactivity

Recombinant Human HGF (294-HG) induces IL-11 secretion in Saos-2 human osteosarcoma cells. The ED50 for this effect is equal to less than 4.00 ng/mL.

Adult Stem Cell-derived Liver Organoids Cultured using Recombinant Human HGF Protein.

Adult stem cell-derived liver organoids were cultured using Cultrex UltiMatrix RGF Basement Membrane Extract (BME001-05) and liver organoid initiation medium, which includes Recombinant Human HGF (Catalog # 294-HG), Recombinant Human BMP-7 (354-BP), Recombinant Human EGF (236-EG), Recombinant Human FGF-10 (345-FG), Recombinant Human Noggin (6057-NG), Recombinant Human R-Spondin 1 (4645-RS), and Recombinant Human Wnt-3a (5036-WN), along with the other reagents listed in the liver organoid initiation medium recipe in the human liver organoid culture protocol. After 3 days, liver organoid expansion medium was added for 7-10 days, followed by liver organoid differentiation medium, which includes Recombinant Human HGF (Catalog # 294-HG), Recombinant Human BMP-7 (354-BP), Recombinant Human EGF (236-EG), and Recombinant Human FGF-19 (969-FG), along with the other reagents listed in the liver organoid differentiation medium recipe in the human liver organoid culture protocol. Differentiated human liver organoids were stained using a (A) Mouse Anti-Human Serum Albumin Monoclonal Antibody (MAB1455; red) and a (B) Goat Anti-Human HNF-3beta Antigen Affinity-purified Polyclonal Antibody (AF2400; red) and counterstained with DAPI (5748; blue).

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 294-HG
Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 294-HG/CF
Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl.
Reconstitution Reconstitute at 100 µg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HGF

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE‑like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1‑5). Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive
disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta  chain. Alternate splicing generates human HGF isoforms that lack the proteinase‑like domain and different numbers of the Kringle domains. Human HGF shares 91%‑94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up‑regulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin‑1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH‑stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

References

  1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
  2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
  3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
  4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
  5. Nakamura, T. et al. (1989) Nature 342:440.
  6. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
  7. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
  8. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
  10. Montesano, R. et al. (1991) Cell 67:901.
  11. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
  12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
  13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
  14. Mineo, R. et al. (1994) Endocrinology 145:4355.
  15. Urbanek, K. et al. (2005) Circ. Res. 97:663.

Long Name

Hepatocyte Growth Factor

Alternate Names

DFNB39, F-TCF, Hepatopoietin A, HGFB, HPTA, SF

Entrez Gene IDs

3082 (Human); 15234 (Mouse); 24446 (Rat); 403441 (Canine); 102133907 (Cynomolgus Monkey); 493705 (Feline)

Gene Symbol

HGF

UniProt

P14210.2

Additional HGF Products

Product Documents for Recombinant Human HGF Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 294-HG (with carrier)
Download SDS 294-HG/CF (carrier free)

Product Specific Notices for Recombinant Human HGF Protein

For research use only

Citations for Recombinant Human HGF Protein

Customer Reviews for Recombinant Human HGF Protein (6)

4.8 out of 5
6 Customer Ratings
5 Stars
83%
4 Stars
17%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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  • Recombinant Human HGF Protein
    Name: Anonymous
    Application: Cell migration/motility
    Verified Customer | Posted 07/25/2023
    Recombinant Human HGF Protein 294-HG
  • Recombinant Human HGF Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 08/20/2021
    Recombinant Human HGF Protein 294-HG
  • Recombinant Human HGF Protein
    Name: Danny Tam
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 12/21/2017
  • Recombinant Human HGF Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 11/16/2017
    A sandwich ELISA was made with MAB694 as the capture and BAF294 as the detection using 294-HG as the immunoassay calibrator.
    Recombinant Human HGF Protein 294-HG
  • Recombinant Human HGF Protein
    Name: Anonymous
    Application: stimulate met activity in cancer cells
    Verified Customer | Posted 09/08/2017
    Used HGF to stimulate met activity in cancer cells and reversed this effect with a small molecule inhibitor
    Recombinant Human HGF Protein 294-HG
  • Recombinant Human HGF Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 01/13/2016

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FAQs for Recombinant Human HGF Protein

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  • Q: Does Recombinant Human HGF Protein (Catalog # 294-HG or 294-HG/CF) contain any tags?

    A: Recombinant Human HGF Protein (Catalog # 294-HG or 294-HG/CF) does not contain any tags. Please refer to the Source section on the product-specific page for sequence information.

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