Human HGF DuoSet ELISA

Catalog # Availability Size / Price Qty
DY294
Ancillary Products Available

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Human HGF ELISA Standard Curve
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Product Details
Procedure
Citations (25)
FAQs
Supplemental Products
Reviews (2)

Human HGF DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
125.0 - 8,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human HGF. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Data Example

Human HGF ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HGF

HGF (Hepatocyte Growth Factor, Scatter Factor) induces the proliferation and migration of epithelial cells, hepatocytes, chondrocytes, keratinocytes, melanocytes, endothelial cells, and many tumor cells. During organogenesis and tissue repair, HGF promotes epithelial/endothelial morphogenesis by inducing cell scattering and branching tubulogenesis. It also supports insulin production by pancreatic beta cells, neuronal survival, and immune tolerance. HGF is secreted as a propeptide that is activated by uPA or HGF Activator at sites of tissue damage. Its signaling through the receptor HGF R/c-MET is enhanced by its prior binding to heparan sulfate proteoglycans. The serum levels of HGF are elevated in a wide range of pathologies including liver damage, acute kidney failure, myocardial infarction, type 1 diabetes, obesity, and cancer, as well as in the synovial fluid of rheumatoid arthritis patients.

Long Name:
Hepatocyte Growth Factor
Entrez Gene IDs:
3082 (Human); 15234 (Mouse); 24446 (Rat); 403441 (Canine); 493705 (Feline)
Alternate Names:
deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

 

Citations for Human HGF DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. Delivery of affordable and scalable encapsulated allogenic/autologous mesenchymal stem cells in coagulated platelet poor plasma for dental pulp regeneration
    Authors: I Angelopoul, C Trigo, MI Ortuzar, J Cuenca, C Brizuela, M Khoury
    Scientific Reports, 2022;12(1):435.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Efficient blockade of locally reciprocated tumor-macrophage signaling using a TAM-avid nanotherapy
    Authors: SJ Wang, R Li, TSC Ng, G Luthria, MJ Oudin, M Prytyskach, RH Kohler, R Weissleder, DA Lauffenbur, MA Miller
    Sci Adv, 2020;6(21):eaaz8521.
    Species: Human
    Sample Types: Cell Lysates
  3. Are the Immune Properties of Mesenchymal Stem Cells from Wharton's Jelly Maintained during Chondrogenic Differentiation?
    Authors: C Voisin, G Cauchois, L Reppel, C Laroye, L Louarn, C Schenowitz, P Sonon, I Poras, V Wang, E D Carosell, N Benkirane-, P Moreau, N Rouas-Frei, D Bensoussan, C Huselstein
    J Clin Med, 2020;9(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Mesenchymal Stem and Stromal Cells Harness Macrophage-Derived Amphiregulin to Maintain Tissue Homeostasis
    Authors: JH Ko, HJ Kim, HJ Jeong, HJ Lee, JY Oh
    Cell Rep, 2020;30(11):3806-3820.e6.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. A Unique Pattern of Mesothelial-Mesenchymal Transition Induced in the Normal Peritoneal Mesothelium by High-Grade Serous Ovarian Cancer
    Authors: M Paku?a, P Uruski, A Niklas, A Wo?niak, D Szpurek, A Tykarski, J Miku?a-Pie, K Ksi??ek
    Cancers (Basel), 2019;11(5):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Hepatic blood flow by perfusion computed tomography as an imaging biomarker for patients with gastric cancer
    Authors: K Shuto, M Mori, C Kosugi, K Narushima, S Nakabayash, T Fujisiro, A Sato, K Hayano, H Shimizu, K Koda
    Oncol Lett, 2019;17(3):3267-3276.
    Species: Human
    Sample Types: Serum
  7. Comparative Analysis of Different Platelet Lysates and Platelet Rich Preparations to Stimulate Tendon Cell Biology: An In Vitro Study
    Authors: F Klatte-Sch, T Schmidt, M Uckert, S Scheffler, U Kalus, M Rojewski, H Schrezenme, A Pruss, B Wildemann
    Int J Mol Sci, 2018;19(1):.
    Species: Human
    Sample Types: Plasma
  8. Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton Jelly mesenchymal stromal cells
    Authors: MS Chen, TJ Wang, HC Lin, B Thierry
    New biotechnology, 2018;0(0):.
    Species: Human
    Sample Types: Platelet Lysates
  9. TGF beta inhibits HGF, FGF7, and FGF10 expression in normal and IPF lung fibroblasts
    Authors: KA Correll, KE Edeen, EF Redente, RL Zemans, BL Edelman, T Danhorn, D Curran-Eve, A Mikels-Vig, RJ Mason
    Physiol Rep, 2018;6(16):e13794.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease
    Authors: J Ikari, AJ Nelson, J Obaid, A Giron-Mart, K Ikari, F Makino, S Iwasawa, Y Gunji, M Farid, X Wang, H Basma, D Demeo, C Feghali-Bo, O Holz, K Rabe, X Liu, SI Rennard
    PLoS ONE, 2017;12(9):e0184039.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. mTORC1-independent autophagy regulates receptor tyrosine kinase phosphorylation in colorectal cancer cells via an mTORC2-mediated mechanism
    Authors: A Lampada, J O'Prey, G Szabadkai, KM Ryan, D Hochhauser, P Salomoni
    Cell Death Differ., 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Effect of 2-octylcyanoacrylate on placenta derived mesenchymal stromal cells on extracellular matrix
    Authors: YJ Chen, L Lankford, S Kabagambe, Z Saenz, P Kumar, D Farmer, A Wang
    Placenta, 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. The Proangiogenic Capabilities of Malignant Ascites Generated by Aggressive Ovarian Tumors
    Authors: J Miku?a-Pie, P Uruski, S Szubert, K Maksin, R Moszy?ski, D Szpurek, A Wo?niak, S Sajdak, A Tykarski, K Ksi??ek
    Biomed Res Int, 2017;2017(0):2592496.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. A Collagen Cardiac Patch Incorporating Alginate Microparticles Permits the Controlled Release of HGF and IGF-1 to Enhance Cardiac Stem Cell Migration and Proliferation
    Authors: Hugh S O'Neill
    J Tissue Eng Regen Med, 2016;0(0):.
  15. Secretory activity of subcutaneous abdominal adipose tissue in male patients with rheumatoid arthritis and osteoarthritis - association with clinical and laboratory data
    Authors: Ewa Kontny
    Reumatologia, 2016;54(5):227-235.
    Species: Human
    Sample Types: Tissue Extracts
  16. Characterization of In Vitro Engineered Human Adipose Tissues: Relevant Adipokine Secretion and Impact of TNF-alpha.
    Authors: Aubin K, Safoine M, Proulx M, Audet-Casgrain M, Côté J, Tetu F, Roy A, Fradette J
    PLoS ONE, 2015;10(9):e0137612.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Transplantation of human adipose tissue-derived stem cells delays clinical onset and prolongs life span in ALS mouse model.
    Authors: Kim, Kwang S, Lee, Hong J, An, Jin, Kim, Yun B, Ra, Jung Cha, Lim, Inja, Kim, Seung U
    Cell Transplant, 2014;23(12):1585-97.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Aqueous two-phase system patterning of detection antibody solutions for cross-reaction-free multiplex ELISA.
    Authors: Frampton, John P, White, Joshua B, Simon, Arlyne B, Tsuei, Michael, Paczesny, Sophie, Takayama, Shuichi
    Sci Rep, 2014;4(0):4878.
    Species: Human
    Sample Types: Plasma
  19. Fibroblasts derived from human pluripotent stem cells activate angiogenic responses in vitro and in vivo.
    Authors: Shamis Y, Silva E, Hewitt K, Brudno Y, Levenberg S, Mooney D, Garlick J
    PLoS ONE, 2013;8(12):e83755.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Heparanase Plays a Dual Role in Driving Hepatocyte Growth Factor (HGF) Signaling by Enhancing HGF Expression and Activity.
    Authors: Ramani VC, Yang Y, Ren Y
    J. Biol. Chem., 2011;286(8):6490-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Angiogenesis and lymphangiogenesis are downregulated in primary breast cancer.
    Authors: Boneberg EM, Legler DF, Hoefer MM, Ohlschlegel C, Steininger H, Fuzesi L, Beer GM, Dupont-Lampert V, Otto F, Senn HJ, Furstenberger G
    Br. J. Cancer, 2009;101(4):605-14.
    Species: Human
    Sample Types: Serum
  22. Influence of microvascular endothelial cells on transcriptional regulation of proximal tubular epithelial cells.
    Authors: Aydin S, Signorelli S, Lechleitner T, Joannidis M, Pleban C, Perco P, Pfaller W, Jennings P
    Am. J. Physiol., Cell Physiol., 2007;294(2):C543-54.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
    Authors: Skibinski G, Elborn JS, Ennis M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L69-76.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Regulation of hepatocyte growth factor secretion by fibroblasts in patients with acute lung injury.
    Authors: Quesnel C, Marchand-Adam S, Fabre A, Marchal-Somme J, Philip I, Lasocki S, Lecon V, Crestani B, Dehoux M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;294(2):L334-43.
    Species: Human
    Sample Types: BALF
  25. Structural basis of hepatocyte growth factor/scatter factor and MET signalling.
    Authors: Gherardi E, Sandin S, Petoukhov MV, Finch J, Youles ME, Ofverstedt LG, Miguel RN, Blundell TL, Vande Woude GF, Skoglund U, Svergun DI
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(11):4046-51.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human HGF DuoSet ELISA
By Rob Knight on 12/09/2021
Sample Tested: Cell culture supernatant

Dermal Fibroblast conditioned medium following stimulation with stem cell derived small extracellular vesicles


Human HGF DuoSet ELISA
By Anonymous on 08/19/2021
Sample Tested: Cell Culture Media