• Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.25 hours or 4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Citrate Plasma (50 uL)
  • Sensitivity
    40 pg/mL
  • Assay Range
    125.0 - 8,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human HGF
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human HGF Immunoassay is a 4.25 or 4.5 hour solid phase ELISA that is designed to measure HGF levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human pro-HGF and antibodies raised against the recombinant factor and has been shown to quantitate recombinant human HGF accurately. Results obtained using natural HGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural HGF.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation23.756.215425.881.2205

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Standard Deviation30.243.223429.475.1264


The recovery of HGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 92 86-101
Citrate Plasma (n=5) 105 99-114
EDTA Plasma (n=5) 100 85-111
Serum (n=5) 99 91-108
To assess the linearity of the assay, five samples of each sample type were spiked with high concentrations of HGF and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human HGF Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: HGF
HGF (Hepatocyte Growth Factor, Scatter Factor) induces the proliferation and migration of epithelial cells, hepatocytes, chondrocytes, keratinocytes, melanocytes, endothelial cells, and many tumor cells. During organogenesis and tissue repair, HGF promotes epithelial/endothelial morphogenesis by inducing cell scattering and branching tubulogenesis. It also supports insulin production by pancreatic beta cells, neuronal survival, and immune tolerance. HGF is secreted as a propeptide that is activated by uPA or HGF Activator at sites of tissue damage. Its signaling through the receptor HGF R/c-MET is enhanced by its prior binding to heparan sulfate proteoglycans. The serum levels of HGF are elevated in a wide range of pathologies including liver damage, acute kidney failure, myocardial infarction, type 1 diabetes, obesity, and cancer, as well as in the synovial fluid of rheumatoid arthritis patients.
    • Long Name
      Hepatocyte Growth Factor
    • Entrez Gene IDs
      3082 (Human); 15234 (Mouse); 403441 (Canine);
    • Alternate Names
      deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGFB; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 150 µL Assay Diluent
    4.   Add 150 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well.
    10.   For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
      For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 1.75 hours.
    11.   Aspirate and wash 4 times.

    12. 200 µL Substrate Solution
    13.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    14. 50 µL Stop Solution
    15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 40
    Filter your results:

    Sample Type
    1. The role of HGF/MET and FGF/FGFR in fibroblast-derived growth stimulation and lapatinib-resistance of esophageal squamous cell carcinoma.
      Authors: Saito S, Morishima K, Ui T, Hoshino H, Matsubara D, Ishikawa S, Aburatani H, Fukayama M, Hosoya Y, Sata N, Lefor A, Yasuda Y, Niki T
      BMC Cancer, 2015;15(0):82.
      Species: Human
      Sample Type: cell culture supernate
    2. Multiple regulatory mechanisms of hepatocyte growth factor expression in malignant cells with a short poly(dA) sequence in the HGF gene promoter.
      Authors: Sakai K, Takeda M, Okamoto I, Nakagawa K, Nishio K
      Oncol Lett, 2015;9(1):405-410.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. Analysis of plasma cytokines and angiogenic factors in patients with pretreated urothelial cancer receiving Pazopanib: the role of circulating interleukin-8 to enhance the prognostic accuracy.
      Authors: Necchi A, Pennati M, Zaffaroni N, Landoni E, Giannatempo P, Raggi D, Schwartz L, Morosi C, Crippa F, Fare E, Nicolai N, Lanocita R, Sava T, Sacco C, Messina C, Ortega C, De Braud F, Salvioni R, Daidone M, Gianni A, Mariani L
      Br J Cancer, 2014;110(1):26-33.
      Species: Human
      Sample Type: Plasma
    4. A pharmacodynamic/pharmacokinetic study of ficlatuzumab in patients with advanced solid tumors and liver metastases.
      Authors: Tabernero J, Elez M, Herranz M, Rico I, Prudkin L, Andreu J, Mateos J, Carreras M, Han M, Gifford J, Credi M, Yin W, Agarwal S, Komarnitsky P, Baselga J
      Clin Cancer Res, 2014;20(10):2793-804.
      Species: Human
      Sample Type: Serum
    5. Clinical outcome, proteome kinetics and angiogenic factors in serum after thermoablation of colorectal liver metastases.
      Authors: Wertenbroek, Marieke, Schepers, Marianne, Kamminga-Rasker, Hannetta, Bottema, Jan T, Muller Kobold, Anneke C, Roelofsen, Han, de Jong, Koert P
      BMC Cancer, 2013;13(1):266.
      Species: Human
      Sample Type: Serum
    6. Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
      Authors: Kinzebach S, Dietz L, Kluter H, Thierse H, Bieback K
      BMC Cell Biol, 2013;14(0):48.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. The role of SDF-1-CXCR4/CXCR7 axis in biological behaviors of adipose tissue-derived mesenchymal stem cells in vitro.
      Authors: Li Q, Zhang A, Tao C, Li X, Jin P
      Biochem Biophys Res Commun, 2013;441(3):675-80.
      Species: Human
      Sample Type: Cell Culture Supernates
    8. Biological, functional and genetic characterization of bone marrow-derived mesenchymal stromal cells from pediatric patients affected by acute lymphoblastic leukemia.
      Authors: Conforti A, Biagini S, Del Bufalo F, Sirleto P, Angioni A, Starc N, Li Pira G, Moretta F, Proia A, Contoli B, Genovese S, Ciardi C, Avanzini M, Rosti V, Lo-Coco F, Locatelli F, Bernardo M
      PLoS ONE, 2013;8(11):e76989.
      Species: Human
      Sample Type: cell culture supernate
    9. Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma.
      Authors: Llovet JM, Pena CE, Lathia CD, Shan M, Meinhardt G, Bruix J, Pallota MG, Zarba JJ, Boyer M, Riordan S, Strickland A, Tebbutt N, Thomson B, Borbath I, De Greve J, Van Laethem J-, Van Steenbergen W, Van Vlierberghe H, Barrios C, Cosme de Oliveira A, Kotzev I, Takov D, Tchernev K, Burak K, Ma M, Metrakos P, Olweny C, Sherman M, Gamargo Garate C, Martinez-Castillo J, Beaugrand M, Bennouna J, Blanc J-, Bronowicki J-, Degos F, Dominguez S, Grange J-, Hillon P, Raoul J-, Seitz J-, Blum H, Buggisch P, Caspary W, Dollinger M, Gerken G, Goke B, Gregor M, Greten T, Haussinger D, Hilgard P, Scherübl J, Scheulen M, Schmid R, Spengler U, Wiest R, Zeuzem S, Arvanitakis C, Germanidis G, Katsos I, Figer A, Stemmer S, Amadori D, Bolondi L, Cognetti F, Craxi A, Farinati F, Gridelli C, Martoni A, Mazzaferro V, Porta C, Ricci S, Sangiovanni A, Santoro A, Trevisani F, Cisnero Garza LE, Gane E, O'Donnell A, Leon J, Lozano A, Jassem J, Rydzewska G, Szawlowski A, Tomczak P, Badulescu F, Miron L, Kubyshkin V, Bruix J, Forner A, Bustamante Schneider J, Diago M, Montero Alvarez JL, Pascual S, Ruíz del Arbol L, Sangro B, Solá R, Tabernero J, Muellhaupt B, Roth A, Jeffry Evans TR, Falk S, Meyer T, Reeves H, Ross P, Befeler A, Boyer T, Britten C, Byrne T, Garcia-Tsao G, Gold P, Goldenberg A, Heuman D, Kennedy P, Koch A, Llovet JM, Marrero J, Schilsky M, Schwartz J, Schwartz M
      Clin. Cancer Res., 2012;18(8):2290-300.
      Species: Human
      Sample Type: Plasma
    10. Comparison of the levels of the growth factors in umbilical cord serum and human milk and its clinical significance.
      Authors: Patki S, Patki U, Patil R, Indumathi S, Kaingade P, Bulbule A, Nikam A, Pishte A
      Cytokine, 2012;59(2):305-8.
      Species: Human
      Sample Type: umbilical cord serum
    11. The secretion pattern of perivascular fat cells is different from that of subcutaneous and visceral fat cells.
      Authors: Rittig K, Dolderer JH, Balletshofer B, Machann J, Schick F, Meile T, Kuper M, Stock UA, Staiger H, Machicao F, Schaller HE, Konigsrainer A, Haring HU, Siegel-Axel DI
      Diabetologia, 2012;55(5):1514-25.
      Species: Human
      Sample Type: Serum
    12. Validation and quality control of ELISAs for the use with human saliva samples.
      Authors: Jaedicke KM, Taylor JJ, Preshaw PM
      J. Immunol. Methods, 2012;377(1):62-5.
      Species: Human
      Sample Type: Saliva
    13. Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
      Authors: Li TS, Cheng K, Malliaras K, Matsushita N, Sun B, Marban L, Zhang Y, Marban E
      Cardiovasc. Res., 2011;89(1):157-65.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
      Authors: Rauch C, Feifel E, Amann EM, Spotl HP, Schennach H, Pfaller W, Gstraunthaler G
      ALTEX, 2011;28(4):305-16.
      Species: Human
      Sample Type: Cell Lysates
    15. Hypoxia-inducible factors in human pulmonary arterial hypertension: a link to the intrinsic myeloid abnormalities.
      Authors: Farha S, Asosingh K, Xu W, Sharp J, George D, Comhair S, Park M, Tang WH, Loyd JE, Theil K, Tubbs R, Hsi E, Lichtin A, Erzurum SC
      Blood, 2011;117(13):3485-93.
      Species: Human
      Sample Type: Plasma
    16. Human stem/progenitor cells from bone marrow enhance glial differentiation of rat neural stem cells: a role for transforming growth factor beta and Notch signaling.
      Authors: Robinson AP, Foraker JE, Ylostalo J, Prockop DJ
      Stem Cells Dev., 2011;20(2):289-300.
      Species: Human
      Sample Type: Cell Culture Supernates
    17. Wnt activity defines colon cancer stem cells and is regulated by the microenvironment.
      Authors: Vermeulen L, De Sousa E Melo F, van der Heijden M, Cameron K, de Jong JH, Borovski T, Tuynman JB, Todaro M, Merz C, Rodermond H, Sprick MR, Kemper K, Richel DJ, Stassi G, Medema JP
      Nat. Cell Biol., 2010;12(5):468-76.
      Species: Human
      Sample Type: Cell Culture Supernates
    18. Impact of serum hepatocyte growth factor on treatment response to epidermal growth factor receptor tyrosine kinase inhibitors in patients with non-small cell lung adenocarcinoma.
      Authors: Kasahara K, Arao T, Sakai K, Matsumoto K, Sakai A, Kimura H, Sone T, Horiike A, Nishio M, Ohira T, Ikeda N, Yamanaka T, Saijo N, Nishio K
      Clin. Cancer Res., 2010;16(18):4616-24.
      Species: Human
      Sample Type: Serum
    19. Safety, pharmacokinetics, and pharmacodynamics of AMG 102, a fully human hepatocyte growth factor-neutralizing monoclonal antibody, in a first-in-human study of patients with advanced solid tumors.
      Authors: Gordon MS, Sweeney CS, Mendelson DS
      Clin. Cancer Res., 2010;16(2):699-710.
      Species: Human
      Sample Type: Plasma
    20. Alternative proteolytic processing of hepatocyte growth factor during wound repair.
      Authors: Buchstein N, Hoffmann D, Smola H, Lang S, Paulsson M, Niemann C, Krieg T, Eming SA
      Am. J. Pathol., 2009;174(6):2116-28.
      Species: Human
      Sample Type: wound exudate
    21. Clinical correlations and prognostic relevance of HGF, VEGF AND FGF expression in Brazilian patients with non-Hodgkin lymphoma.
      Authors: Etto L, Lacerda E, Baiocchi O, Silva V, Dalboni M, Alves A, Silva M, Vettore A, Colleoni G
      Leuk. Lymphoma, 2008;49(2):257-64.
      Species: Human
      Sample Type: Serum
    22. Abnormal cytokine production by bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.
      Authors: Zdzisinska B, Bojarska-Junak A, Dmoszynska A, Kandefer-Szerszen M
      Arch. Immunol. Ther. Exp. (Warsz.), 2008;56(3):207-21.
      Species: Human
      Sample Type: Cell Culture Supernates
    23. IFATS Series: FGF-2-induced HGF Secretion By Adipose-Derived Stromal Cells Inhibits Post-Injury Fibrogenesis Through A JNK-Dependent Mechanism.
      Authors: Suga H, Eto H, Shigeura T, Inoue K, Aoi N, Kato H, Nishimura S, Manabe I, Gonda K, Yoshimura K
      Stem Cells, 2008;0(0):.
      Species: Human
      Sample Type: Conditioned media
    24. Impairment of the antifibrotic effect of hepatocyte growth factor in lung fibroblasts from African Americans: possible role in systemic sclerosis.
      Authors: Bogatkevich GS, Ludwicka-Bradley A, Highland KB, Hant F, Nietert PJ, Singleton CB, Feghali-Bostwick CA, Silver RM
      Arthritis Rheum., 2007;56(7):2432-42.
      Species: Human
      Sample Type: Cell Culture Supernates
    25. Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
      Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U
      Stem Cells, 2007;25(8):1862-71.
      Species: Human
      Sample Type: Cell Culture Supernates
    26. A fusion protein of hepatocyte growth factor for immobilization to collagen.
      Authors: Kitajima T, Terai H, Ito Y
      Biomaterials, 2007;28(11):1989-97.
      Species: Human
      Sample Type: recombinant HGF
    27. Hepatocyte growth factor levels in gingival crevicular fluid in health, disease, and after treatment.
      Authors: Nagaraja C, Pradeep AR
      J. Periodontol., 2007;78(4):742-7.
      Species: Human
      Sample Type: Gingival Crevicular Fluid (GCF)
    28. Platelet-derived growth factor-BB controls epithelial tumor phenotype by differential growth factor regulation in stromal cells.
      Authors: Lederle W, Stark HJ, Skobe M, Fusenig NE, Mueller MM
      Am. J. Pathol., 2006;169(5):1767-83.
      Species: Human
      Sample Type: Cell Culture Supernates
    29. Humoral and cellular factors responsible for coronary collateral formation.
      Authors: Sherman JA, Hall A, Malenka DJ, De Muinck ED, Simons M
      Am. J. Cardiol., 2006;98(9):1194-7.
      Species: Human
      Sample Type: Plasma
    30. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
      Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
      J. Immunol., 2005;175(9):6177-89.
      Species: Human
      Sample Type: Cell Culture Supernates
    31. Relation of various plasma growth factor levels in patients with stable angina pectoris and total occlusion of a coronary artery to the degree of coronary collaterals.
      Authors: Briguori C, Testa U, Colombo A, Petrucci E, Condorelli G, Airoldi F, Peschle C, Condorelli G
      Am. J. Cardiol., 2005;97(4):472-6.
      Species: Human
      Sample Type: Serum
    32. Hepatocyte growth factor in polymorphonuclear leukocytes is increased in patients with systemic inflammatory response syndrome.
      Authors: Matsushima A, Ogura H, Koh T, Fujita K, Yoshiya K, Sumi Y, Hosotsubo H, Kuwagata Y, Tanaka H, Shimazu T, Sugimoto H
      J Trauma, 2004;56(2):259-64.
      Species: Human
      Sample Type: Plasma
    33. Hepatocyte growth factor in the vitreous fluid of patients with proliferative diabetic retinopathy: its relationship with vascular endothelial growth factor and retinopathy activity.
      Authors: Simo R, Lecube A, Garcia-Arumi J, Carrasco E, Hernandez C
      Diabetes Care, 2004;27(1):287-8.
      Species: Human
      Sample Type: Vitreous Humor
    34. Hepatocyte growth factor and its role in the pathogenesis of retinal detachment.
      Authors: Jin M, Chen Y, He S, Ryan SJ, Hinton DR
      Invest. Ophthalmol. Vis. Sci., 2004;45(1):323-9.
      Species: Rabbit
      Sample Type: Vitreous Humor
    35. Exercise acutely increases circulating endothelial progenitor cells and monocyte-/macrophage-derived angiogenic cells.
      Authors: Rehman J, Li J, Parvathaneni L, Karlsson G, Panchal VR, Temm CJ, Mahenthiran J, March KL
      J. Am. Coll. Cardiol., 2004;43(12):2314-8.
      Species: Human
      Sample Type: Plasma
    36. Differential macrophage infiltration in early and advanced endometriosis and adjacent peritoneum.
      Authors: Khan KN, Masuzaki H, Fujishita A, Kitajima M, Sekine I, Ishimaru T
      Fertil. Steril., 2004;81(3):652-61.
      Species: Human
      Sample Type: Cell Culture Supernates
    37. Association of interleukin-6 and estradiol with hepatocyte growth factor in peritoneal fluid of women with endometriosis.
      Authors: Khan KN, Masuzaki H, Fujishita A, Hamasaki T, Kitajima M, Hasuo A, Miyamura Y, Ishimaru T
      Acta Obstet Gynecol Scand, 2002;81(8):764-71.
      Species: Human
      Sample Type: Peritoneal Fluid
    38. Exhaled breath condensate and serum levels of hepatocyte growth factor in pneumonia.
      Authors: Nayeri F, Millinger E, Nilsson I, Zetterström, Brudin L, Forsberg P
      Respir Med, 2002;96(2):115-9.
      Species: Human
      Sample Type: Exhaled Breath Condensate (EBC)
    39. Hepatocyte growth factor (HGF) system in gingiva: HGF activator expression by gingival epithelial cells.
      Authors: Ohshima M, Sakai A, Sawamoto Y, Seki K, Ito K, Otsuka K
      J Oral Sci, 2002;44(3):129-34.
      Species: Human
      Sample Type: Gingival Crevicular Fluid (GCF)
    40. Selective plasma filtration for treatment of fulminant hepatic failure induced by D-galactosamine in a pig model.
      Authors: Ho DW, Fan ST, To J, Woo YH, Zhang Z, Lau C, Wong J
      Gut, 2002;50(6):869-76.
      Species: Porcine
      Sample Type: Plasma
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