Human HGF R/c-MET APC-conjugated Antibody

(2 citations)
(1 Review)
  • Species Reactivity
  • Specificity
    Detects human HGF R/c-MET.
  • Source
    Monoclonal Mouse IgG1 Clone # 95106
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human HGF R/c-MET
    Accession # P08581
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of HGF R/c‑MET in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA‑MB‑231 human breast cancer cell line was stained with Mouse Anti-Human HGF R/c‑MET APC‑conjugated Monoclonal Antibody (Catalog # FAB3582A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: HGF R/c-MET

HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta -propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain (3, 4). Proteolysis and alternate splicing generate additional forms of human HGF R which either lack of the kinase domain, consist of secreted extracellular domains, or are deficient in proteolytic separation of the alpha and beta chains (5-7). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 8). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (9, 10). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (11). In the absence of ligand, HGF R forms non-covalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, Integrin alpha 6/ beta 4, Plexins B1, 2, 3, and MSP R/Ron (12-19). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (12-19). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (12, 16, 17). Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells (20). Genetic polymorphisms, chromosomal translocation, over-expression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, human HGF R shares 86-88% amino acid sequence identity with canine, mouse, and rat HGF R.

  • References:
    1. Birchmeier, C. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:915.
    2. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
    3. Gherardi, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100:12039.
    4. Park, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:6379.
    5. Crepaldi, T. et al. (1994) J. Biol. Chem. 269:1750.
    6. Prat, M. et al. (1991) Mol. Cell. Biol. 12:5954.
    7. Rodrigues, G.A. et al. (1991) Mol. Cell. Biol. 11:2962.
    8. Kong-Beltran, M. et al. (2004) Cancer Cell 6:75.
    9. Naldini, L. et al. (1991) Mol. Cell. Biol. 11:1793.
    10. Ponzetto, C. et al. (1994) Cell 77:261.
    11. Jeffers, M. et al. (1997) Mol. Cell. Biol. 17:799.
    12. Orian-Rousseau, V. et al. (2002) Genes Dev. 16:3074.
    13. Klosek, S.K. et al. (2005) Biochem. Biophys. Res. Commun. 336:408.
    14. Jo, M. et al. (2000) J. Biol. Chem. 275:8806.
    15. Wang, X. et al. (2002) Mol. Cell 9:411.
    16. Trusolino, L. et al. (2001) Cell 107:643.
    17. Giordano, S. et al. (2002) Nat. Cell Biol. 4:720.
    18. Conrotto, P. et al. (2004) Oncogene 23:5131.
    19. Follenzi, A. et al. (2000) Oncogene 19:3041.
    20. Sonnenberg, E. et al. (1993) J. Cell Biol. 123:223.
  • Long Name:
    Hepatocyte Growth Factor Receptor
  • Entrez Gene IDs:
    4233 (Human); 17295 (Mouse)
  • Alternate Names:
    AUTS9; cMET; c-MET; EC 2.7.10; EC; hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; HGFR; Met (c-Met); met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; MET; oncogene MET; Proto-oncogene c-Met; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
    Authors: Kinzebach S, Dietz L, Kluter H, Thierse H, Bieback K
    BMC Cell Biol, 2013;14(0):48.
    Species: Human
    Sample Type: whole cells
    Application: Flow
  2. Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay.
    Authors: Baccelli I, Schneeweiss A, Riethdorf S, Stenzinger A, Schillert A, Vogel V, Klein C, Saini M, Bauerle T, Wallwiener M, Holland-Letz T, Hofner T, Sprick M, Scharpff M, Marme F, Sinn H, Pantel K, Weichert W, Trumpp A
    Nat Biotechnol, 2013;31(6):539-44.
    Species: Human
    Sample Type: whole cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 5  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Human HGF R/c‑MET APC‑conjugated Antibody FAB3582A
Very Good
 Flow Human Anonymous 10/26/2015
Image Details
Flow Cytometry Human HGF R/c‑MET APC‑conjugated Antibody FAB3582A
Flow Cytometry: Human HGF R/c‑MET APC‑conjugated Antibody [FAB3582A]


ApplicationFlow Cytometry
Sample TestedHuman keratinocytes

Other Experimental Details

Other Experimental DetailsSpecificity: Specific
Sensitivity: Sensitive
Buffer: 10%FBS in PBS
Dilution: 10uL/ sample of 1 x 10^5 cells

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