Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Western Blot, Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 95106
View all HGFR/c-MET Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human HGF R/c-MET
Glu25-Thr932
Accession # P08581

Specificity

Detects human HGF R/c-MET.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human HGFR/c-MET PE-conjugated Antibody

Detection of HGF R/c-MET antibody in MDA-MB-231 Human Cell Line antibody by Flow Cytometry.

Detection of HGF R/c‑MET in MDA‑MB‑231 Human Cell Line by Flow Cytometry.

MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human HGF R/c-MET PE-conjugated Monoclonal Antibody (Catalog # FAB3582P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of HGFR/c-MET by Flow Cytometry

Detection of HGFR/c-MET by Flow Cytometry

Increased PD-L1 concentration on the cell surface is mesenchymal–epithelial transition (Met)-receptor-dependent. Detroit 562 cells were transfected with two different siRNA constructs specific for the Met receptor (Met siRNA I and II) or a control siRNA. Forty-eight hours after transfection, cells were treated with 50 ng/mL HGF or remained untreated. Cells were subjected to flow cytometry after an additional 48 h using a phycoerythrin (PE)-conjugated Met specific antibody in (a–c), an allophycocyanin (APC)-conjugated PD-L1 specific antibody in (d–f) or the corresponding isotype controls (light-colored curves). Panels (a,b,d,e) show histograms of cells transfected with the indicated siRNAs, (c) and (f) are the median fluorescence of the corresponding histograms shown in (a,b,d,e) (isotype controls subtracted). One typical result out of six experiments is shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33233528), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human HGFR/c-MET PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: MDA‑MB‑231 human breast cancer cell line

Reviewed Applications

Read 2 reviews rated 4.5 using FAB3582P in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: HGFR/c-MET

HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta -propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain (3, 4). Proteolysis and alternate splicing generate additional forms of human HGF R which either lack of the kinase domain, consist of secreted extracellular domains, or are deficient in proteolytic separation of the alpha and beta chains (5-7). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 8). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (9, 10). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (11). In the absence of ligand, HGF R forms non-covalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, Integrin alpha 6/ beta 4, Plexins B1, 2, 3, and MSP R/Ron (12-19). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (12-19). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (12, 16, 17). Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells (20). Genetic polymorphisms, chromosomal translocation, over-expression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, human HGF R shares 86-88% amino acid sequence identity with canine, mouse, and rat HGF R.

References

  1. Birchmeier, C. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:915.
  2. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  3. Gherardi, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100:12039.
  4. Park, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:6379.
  5. Crepaldi, T. et al. (1994) J. Biol. Chem. 269:1750.
  6. Prat, M. et al. (1991) Mol. Cell. Biol. 12:5954.
  7. Rodrigues, G.A. et al. (1991) Mol. Cell. Biol. 11:2962.
  8. Kong-Beltran, M. et al. (2004) Cancer Cell 6:75.
  9. Naldini, L. et al. (1991) Mol. Cell. Biol. 11:1793.
  10. Ponzetto, C. et al. (1994) Cell 77:261.
  11. Jeffers, M. et al. (1997) Mol. Cell. Biol. 17:799.
  12. Orian-Rousseau, V. et al. (2002) Genes Dev. 16:3074.
  13. Klosek, S.K. et al. (2005) Biochem. Biophys. Res. Commun. 336:408.
  14. Jo, M. et al. (2000) J. Biol. Chem. 275:8806.
  15. Wang, X. et al. (2002) Mol. Cell 9:411.
  16. Trusolino, L. et al. (2001) Cell 107:643.
  17. Giordano, S. et al. (2002) Nat. Cell Biol. 4:720.
  18. Conrotto, P. et al. (2004) Oncogene 23:5131.
  19. Follenzi, A. et al. (2000) Oncogene 19:3041.
  20. Sonnenberg, E. et al. (1993) J. Cell Biol. 123:223.

Long Name

Hepatocyte Growth Factor Receptor

Alternate Names

c-MET, cMET, HGF R, MET

Entrez Gene IDs

4233 (Human); 17295 (Mouse); 102123512 (Cynomolgus Monkey)

Gene Symbol

MET

UniProt

P08581

Additional HGFR/c-MET Products

Product Documents for Human HGFR/c-MET PE-conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human HGFR/c-MET PE-conjugated Antibody

For research use only

Citations for Human HGFR/c-MET PE-conjugated Antibody

Customer Reviews for Human HGFR/c-MET PE-conjugated Antibody (2)

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  • Human HGFR/c-MET PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: NCI-H226 lung squamous cell carcinoma cells
    Species: Human
    Verified Customer | Posted 12/23/2020
    Detection of human HGFR/c-MET on NCI-H226 lung squamous cell carcinoma cells. NCI-H226 cells were treated with the Human HGFR/c-MET PE-conjugated Antibody (catalog # FAB3582P) (BLUE) or PE-conjugated Mouse IgG1isotype control (RED)
    Human HGFR/c-MET PE-conjugated Antibody FAB3582P
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 24013625
    Species: Human
    Verified Customer | Posted 01/26/2015

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