Human HGFR/c-MET PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3582P
Detection of HGF R/c‑MET in MDA‑MB‑231 Human Cell Line by Flow Cytometry.
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Product Details
Citations (10)
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Human HGFR/c-MET PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human HGF R/c-MET.
Source
Monoclonal Mouse IgG1 Clone # 95106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HGF R/c-MET
Glu25-Thr932
Accession # P08581
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of HGF R/c-MET antibody in MDA-MB-231 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of HGF R/c‑MET in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human HGF R/c-MET PE-conjugated Monoclonal Antibody (Catalog # FAB3582P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: HGFR/c-MET

HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta -propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain (3, 4). Proteolysis and alternate splicing generate additional forms of human HGF R which either lack of the kinase domain, consist of secreted extracellular domains, or are deficient in proteolytic separation of the alpha and beta chains (5-7). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 8). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (9, 10). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (11). In the absence of ligand, HGF R forms non-covalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, Integrin alpha 6/ beta 4, Plexins B1, 2, 3, and MSP R/Ron (12-19). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (12-19). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (12, 16, 17). Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells (20). Genetic polymorphisms, chromosomal translocation, over-expression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, human HGF R shares 86-88% amino acid sequence identity with canine, mouse, and rat HGF R.

References
  1. Birchmeier, C. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:915.
  2. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  3. Gherardi, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100:12039.
  4. Park, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:6379.
  5. Crepaldi, T. et al. (1994) J. Biol. Chem. 269:1750.
  6. Prat, M. et al. (1991) Mol. Cell. Biol. 12:5954.
  7. Rodrigues, G.A. et al. (1991) Mol. Cell. Biol. 11:2962.
  8. Kong-Beltran, M. et al. (2004) Cancer Cell 6:75.
  9. Naldini, L. et al. (1991) Mol. Cell. Biol. 11:1793.
  10. Ponzetto, C. et al. (1994) Cell 77:261.
  11. Jeffers, M. et al. (1997) Mol. Cell. Biol. 17:799.
  12. Orian-Rousseau, V. et al. (2002) Genes Dev. 16:3074.
  13. Klosek, S.K. et al. (2005) Biochem. Biophys. Res. Commun. 336:408.
  14. Jo, M. et al. (2000) J. Biol. Chem. 275:8806.
  15. Wang, X. et al. (2002) Mol. Cell 9:411.
  16. Trusolino, L. et al. (2001) Cell 107:643.
  17. Giordano, S. et al. (2002) Nat. Cell Biol. 4:720.
  18. Conrotto, P. et al. (2004) Oncogene 23:5131.
  19. Follenzi, A. et al. (2000) Oncogene 19:3041.
  20. Sonnenberg, E. et al. (1993) J. Cell Biol. 123:223.
Long Name
Hepatocyte Growth Factor Receptor
Entrez Gene IDs
4233 (Human); 17295 (Mouse)
Alternate Names
AUTS9; cMET; c-MET; EC 2.7.10; EC 2.7.10.1; hepatocyte growth factor receptor; HGF R; HGF receptor; HGF/SF receptor; HGFR; Met (c-Met); met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; MET; oncogene MET; Proto-oncogene c-Met; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met

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Citations for Human HGFR/c-MET PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Endosomal LC3C-pathway selectively targets plasma membrane cargo for autophagic degradation
    Authors: PP Coelho, GG Hesketh, A Pedersen, E Kuzmin, AN Fortier, ES Bell, CDH Ratcliffe, AC Gingras, M Park
    Nature Communications, 2022;13(1):3812.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Efficient blockade of locally reciprocated tumor-macrophage signaling using a TAM-avid nanotherapy
    Authors: SJ Wang, R Li, TSC Ng, G Luthria, MJ Oudin, M Prytyskach, RH Kohler, R Weissleder, DA Lauffenbur, MA Miller
    Sci Adv, 2020;6(21):eaaz8521.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. HGF-Induced PD-L1 Expression in Head and Neck Cancer: Preclinical and Clinical Findings
    Authors: V Boschert, J Teusch, A Aljasem, P Schmucker, N Klenk, A Straub, M Bittrich, A Seher, C Linz, UDA Müller-Ric, S Hartmann
    Int J Mol Sci, 2020;21(22):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Combination therapy with c-met inhibitor and TRAIL enhances apoptosis in dedifferentiated liposarcoma patient-derived cells
    Authors: EB Jo, YS Lee, H Lee, JB Park, H Park, YL Choi, D Hong, SJ Kim
    BMC Cancer, 2019;19(1):496.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Ezrin promotes stem cell properties in pancreatic ductal adenocarcinoma
    Authors: VR Penchev, YT Chang, A Begum, T Ewachiw, C Gocke, J Li, RH McMillan, Q Wang, R Anders, L Marchionni, A Maitra, A Uren, Z Rasheed, W Matsui
    Mol. Cancer Res., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. An essential receptor for adeno-associated virus infection.
    Authors: Pillay S, Meyer N, Puschnik A, Davulcu O, Diep J, Ishikawa Y, Jae L, Wosen J, Nagamine C, Chapman M, Carette J
    Nature, 2016;530(7588):108-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Deregulated hepsin protease activity confers oncogenicity by concomitantly augmenting HGF/MET signalling and disrupting epithelial cohesion.
    Authors: Tervonen T, Belitskin D, Pant S, Englund J, Marques E, Ala-Hongisto H, Nevalaita L, Sihto H, Heikkila P, Leidenius M, Hewitson K, Ramachandra M, Moilanen A, Joensuu H, Kovanen P, Poso A, Klefstrom J
    Oncogene, 2015;0(0):ePub.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Impact of Cell-surface Antigen Expression on Target Engagement and Function of an Epidermal Growth Factor Receptor x c-MET Bispecific Antibody.
    Authors: Jarantow S, Bushey B, Pardinas J, Boakye K, Lacy E, Sanders R, Sepulveda M, Moores S, Chiu M
    J Biol Chem, 2015;290(41):24689-704.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R.
    Authors: Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Holler C, Wacheck V, Pehamberger H, Valent P
    PLoS ONE, 2014;9(1):e84417.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Three-dimensional lung tumor microenvironment modulates therapeutic compound responsiveness in vitro--implication for drug development.
    Authors: Ekert, Jason E, Johnson, Kjell, Strake, Brandy, Pardinas, Jose, Jarantow, Stephen, Perkinson, Robert, Colter, David C
    PLoS ONE, 2014;9(3):e92248.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human HGFR/c-MET PE-conjugated Antibody
By Anonymous on 12/23/2020
Application: Flow Sample Tested: NCI-H226 lung squamous cell carcinoma cells Species: Human

Detection of human HGFR/c-MET on NCI-H226 lung squamous cell carcinoma cells. NCI-H226 cells were treated with the Human HGFR/c-MET PE-conjugated Antibody (catalog # FAB3582P) (BLUE) or PE-conjugated Mouse IgG1isotype control (RED)