Human HGFR/c-MET APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3582A
Detection of HGF R/c‑MET in MDA‑MB‑231 Human Cell Line by Flow Cytometry.
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Product Details
Citations (9)
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Reviews (1)

Human HGFR/c-MET APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human HGF R/c-MET.
Source
Monoclonal Mouse IgG1 Clone # 95106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HGF R/c-MET
Glu25-Thr932
Accession # P08581
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of HGF R/c-MET antibody in MDA-MB-231 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of HGF R/c‑MET in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human HGF R/c-MET APC-conjugated Monoclonal Antibody (Catalog # FAB3582A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: HGFR/c-MET

HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta -propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain (3, 4). Proteolysis and alternate splicing generate additional forms of human HGF R which either lack of the kinase domain, consist of secreted extracellular domains, or are deficient in proteolytic separation of the alpha and beta chains (5-7). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 8). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (9, 10). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (11). In the absence of ligand, HGF R forms non-covalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, Integrin alpha 6/ beta 4, Plexins B1, 2, 3, and MSP R/Ron (12-19). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (12-19). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (12, 16, 17). Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells (20). Genetic polymorphisms, chromosomal translocation, over-expression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, human HGF R shares 86-88% amino acid sequence identity with canine, mouse, and rat HGF R.

References
  1. Birchmeier, C. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:915.
  2. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  3. Gherardi, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100:12039.
  4. Park, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:6379.
  5. Crepaldi, T. et al. (1994) J. Biol. Chem. 269:1750.
  6. Prat, M. et al. (1991) Mol. Cell. Biol. 12:5954.
  7. Rodrigues, G.A. et al. (1991) Mol. Cell. Biol. 11:2962.
  8. Kong-Beltran, M. et al. (2004) Cancer Cell 6:75.
  9. Naldini, L. et al. (1991) Mol. Cell. Biol. 11:1793.
  10. Ponzetto, C. et al. (1994) Cell 77:261.
  11. Jeffers, M. et al. (1997) Mol. Cell. Biol. 17:799.
  12. Orian-Rousseau, V. et al. (2002) Genes Dev. 16:3074.
  13. Klosek, S.K. et al. (2005) Biochem. Biophys. Res. Commun. 336:408.
  14. Jo, M. et al. (2000) J. Biol. Chem. 275:8806.
  15. Wang, X. et al. (2002) Mol. Cell 9:411.
  16. Trusolino, L. et al. (2001) Cell 107:643.
  17. Giordano, S. et al. (2002) Nat. Cell Biol. 4:720.
  18. Conrotto, P. et al. (2004) Oncogene 23:5131.
  19. Follenzi, A. et al. (2000) Oncogene 19:3041.
  20. Sonnenberg, E. et al. (1993) J. Cell Biol. 123:223.
Long Name
Hepatocyte Growth Factor Receptor
Entrez Gene IDs
4233 (Human); 17295 (Mouse)
Alternate Names
AUTS9; cMET; c-MET; EC 2.7.10; EC 2.7.10.1; hepatocyte growth factor receptor; HGF R; HGF receptor; HGF/SF receptor; HGFR; Met (c-Met); met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; MET; oncogene MET; Proto-oncogene c-Met; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met

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Citations for Human HGFR/c-MET APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Targeting HGF/c-Met Axis Decreases Circulating Regulatory T Cells Accumulation in Gastric Cancer Patients
    Authors: J Palle, L Hirsch, A Lapeyre-Pr, D Malka, M Bourhis, S Pernot, E Marcheteau, T Voron, F Castan, A Lacotte, N Benhamouda, C Tanchot, E François, F Ghiringhel, C de la Fouc, A Zaanan, E Tartour, J Taieb, M Terme
    Cancers, 2021;13(21):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. CX3CL1 Overexpression Prevents the Formation of Lung Metastases in Trastuzumab-Treated MDA-MB-453-Based Humanized Tumor Mice (HTM)
    Authors: AK Wege, TF Dreyer, A Teoman, O Ortmann, G Brockhoff, H Bronger
    Cancers, 2021;13(10):.
    Species: Xenograft
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Biologically driven cut-off definition of lymphocyte ratios in metastatic breast cancer and association with exosomal subpopulations and prognosis
    Authors: L Gerratana, D Basile, B Toffoletto, M Bulfoni, S Zago, A Magini, M Lera, G Pelizzari, P Parisse, L Casalis, MG Vitale, V Fanotto, M Bonotto, F Caponnetto, M Bartoletti, C Lisanti, AM Minisini, C Emiliani, C Di Loreto, G Fasola, F Curcio, AP Beltrami, D Cesselli, F Puglisi
    Sci Rep, 2020;10(1):7010.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Induction of MET Receptor Tyrosine Kinase Down-regulation through Antibody-mediated Receptor Clustering
    Authors: W Li, A Dick, F Lu, H Zhang, H Sun
    Sci Rep, 2019;9(1):1988.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Premyogenic progenitors derived from human pluripotent stem cells expand in floating culture and differentiate into transplantable myogenic progenitors
    Authors: F Sakai-Take, A Narita, S Masuda, T Wakamatsu, N Watanabe, T Nishiyama, K Nogami, M Blanc, S Takeda, Y Miyagoe-Su
    Sci Rep, 2018;8(1):6555.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Reviving oncogenic addiction to MET bypassed by BRAF (G469A) mutation
    Authors: AR Virzì, A Gentile, S Benvenuti, PM Comoglio
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. GAA Deficiency in Pompe Disease Is Alleviated by Exon Inclusion in iPSC-Derived Skeletal Muscle Cells
    Authors: E van der Wa, AJ Bergsma, TJM van Gestel, SLM In 't Groe, H Zaehres, MJ Araúzo-Bra, HR Schöler, AT van der Pl, WWMP Pijnappel
    Mol Ther Nucleic Acids, 2017;7(0):101-115.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
    Authors: Kinzebach S, Dietz L, Kluter H, Thierse H, Bieback K
    BMC Cell Biol, 2013;14(0):48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay.
    Authors: Baccelli I, Schneeweiss A, Riethdorf S, Stenzinger A, Schillert A, Vogel V, Klein C, Saini M, Bauerle T, Wallwiener M, Holland-Letz T, Hofner T, Sprick M, Scharpff M, Marme F, Sinn H, Pantel K, Weichert W, Trumpp A
    Nat Biotechnol, 2013;31(6):539-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human HGF R/c‑MET APC‑conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: Human keratinocytes Species: Human

Specificity: Specific
Sensitivity: Sensitive
Buffer: 10%FBS in PBS
Dilution: 10uL/ sample of 1 x 10^5 cells