Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Hydroxyacid Oxidase-1/HAO-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Hydroxyacid Oxidase-1/HAO-1
Leu2-Ile370
Accession # Q9UJM8

Specificity

Detects human Hydroxyacid Oxidase-1/HAO-1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Hydroxyacid Oxidase‑1/HAO‑1 Antibody

Detection of Human Hydroxyacid Oxidase-1/HAO-1 antibody by Western Blot.

Detection of Human Hydroxyacid Oxidase‑1/HAO‑1 by Western Blot.

Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human Hydroxyacid Oxidase-1/HAO-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6197) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Hydroxyacid Oxidase-1/HAO-1 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Hydroxyacid Oxidase‑1/HAO‑1 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Human liver tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Hydroxyacid Oxidase-1/HAO-1

Glycolate oxidase is a member of the superfamily of the alpha ‑hydroxy acid oxidases (HAO), enzymes that are present in both plants and animals (1). It catalyzes the FMN-mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide (2, 3). The co‑factor, FMN, is tightly bound but not covalently linked to the protein. In humans and other vertebrates, HAOs are found primarily in the peroxisomes of liver, kidney, and pancreas. Three HAOs have been identified in humans (4). HAO-1 is most highly expressed in liver and pancreas and is most active on two-carbon substrates such as glycolate. HAO-2 is expressed in liver and kidney and has greater activity against long-chain alpha ‑hydroxy acid substrates such as 2‑hydroxypalmitate. HAO-3 is expressed primarily in the pancreas. Recently, HAO has been identified as a major contributor to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones (5).

References

  1. Caroline, V. et al. (2007) Arch. Biochem. Biophys. 465:410.
  2. Murray, M.S. et al. (2008) Biochemistry, 47:2439.
  3. Pennati, A. and G. Gadda. (2009) J. Biol. Chem. 284:31214.
  4. Jones, J.M. et al. (2000) J. Biol. Chem. 275:12590.
  5. Monico, G.C. et al. (2002) Kidney Int. 62:392.

Alternate Names

GOX, HAO-1, HAO1, HAOX1, Hydroxyacid Oxidase1

Entrez Gene IDs

54363 (Human)

Gene Symbol

HAO1

UniProt

Q9UJM8 (Human)

Additional Hydroxyacid Oxidase-1/HAO-1 Products

Product Documents for Human Hydroxyacid Oxidase‑1/HAO‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Hydroxyacid Oxidase‑1/HAO‑1 Antibody

For research use only

Related Research Areas

Oxidases and Oxygenases
Redox Enzymes

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