Human IFN-alpha / beta  R2 Antibody

Catalog # Availability Size / Price Qty
IFN-beta  Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN-alpha /  beta  R2 Antibody.
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Product Details
Citations (2)
Supplemental Products

Human IFN-alpha / beta  R2 Antibody Summary

Species Reactivity
Detects human IFN‑ alpha / beta  R2 in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 493715
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line, NS0-derived recombinant human IFN‑ alpha / beta  R2
Accession # P48551
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Measured by its ability to neutralize IFN‑ beta inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. The Neutralization Dose (ND50) is typically 4-20 µg/mL in the presence of 30 pg/mL Recombinant Human IFN‑ beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IFN-beta  Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN-alpha /  beta  R2 Antibody. View Larger

IFN-beta Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN-alpha / beta R2 Antibody. Recombinant Human IFN-beta (Catalog # 8499-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-beta (30 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IFN-a/ beta R2 Monoclonal Antibody (Catalog # MAB4015). The ND50 is typically 4-20 µg/mL.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha/beta R2

IFN-alpha / beta R2, also known as IFNAR2, is a 100 kDa glycoprotein in the class II cytokine receptor family. These proteins form heterodimeric receptor complexes that transduce signals from the interferon, IL-10, and IL-28 families of cytokines (1, 2). IFN-alpha / beta R2, in association with IFN-alpha / beta R1, is required for mediating the antiviral, antiproliferative, and apoptotic effects of the type I interferons IFN-alpha and IFN-beta. IFN-alpha / beta R2 is the principal ligand binding subunit of the receptor. Ligand binding is stabilized by the subsequent association with IFN-alpha / beta R1, resulting in the formation of a signaling ternary receptor complex (3, 4). Mature human IFN-alpha / beta R2 consists of a 217 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, a 21 aa transmembrane segment, and a 251 aa cytoplasmic domain. Alternate splicing generates a secreted isoform that corresponds to the ECD and a 50 kDa transmembrane isoform with a substituted and truncated cytoplasmic region (5, 6). The short isoform is impaired in its ability to activate signaling molecules and functions as a dominant negative receptor subunit (7-9). IFN-alpha / beta R2 is also subject to presenilin-dependent intramembrane proteolysis, resulting in the liberation of nearly the entire ECD as well as the cytoplasmic domain which migrates to the nucleus and can inhibit gene transcription (10). High concentrations of soluble IFN-alpha / beta R2 bind and neutralize IFN-alpha and IFN-beta, while lower concentrations prolong the antiviral activity of circulating IFN-beta but not IFN-alpha (11). Human but not mouse IFN-alpha / beta R2 constitutively associates with STAT4, which may account for species specific differences observed in type I interferon responses (12). Within the ECD, human IFN-alpha / beta R2 shares 63%, 60%, and 48% aa sequence identity with bovine, mouse, and ovine IFN-alpha / beta R2, respectively.

  1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Lamken, P. et al. (2004) J. Mol. Biol. 341:303.
  4. Arduini, R.M. et al. (1999) Prot. Sci. 8:1867.
  5. Lutfalla, G. et al. (1995) EMBO J. 14:5100.
  6. Novick, D. et al. (1995) J. Leukocyte Biol. 57:712.
  7. Pfeffer, L.M. et al. (1997) J. Biol. Chem. 272:11002.
  8. Gazziola, C. et al. (2005) Int. J. Oncol. 26:129.
  9. Kotenko, S.V. and S. Pestka (2000) Oncogene 19:2557.
  10. Saleh, A.Z.M. et al. (2004) Oncogene 23:7076.
  11. McKenna, S.D. et al. (2004) J. Interferon Cytokine Res. 24:119.
  12. Tyler, D.R. et al. (2007) Mol. Immunol. 44:1864.
Long Name
Interferon alpha/beta Receptor 2
Entrez Gene IDs
3455 (Human); 15976 (Mouse); 102140083 (Cynomolgus Monkey)
Alternate Names
beta receptor10; human interferon alpha; human interferon alpha/beta receptor; IFNABR; IFN-alpha binding protein; IFN-alpha/beta R2; IFN-alpha/beta receptor 2; IFN-alpha-REC; IFNAR2; IFN-aR2; IFNARB; IFNbR2; IFN-bR2; IFN-R; IFN-R-2; interferon (alpha, beta and omega) receptor 2; Interferon alpha binding protein; interferon alpha/beta receptor 2; interferon-alpha/beta receptor beta chain; Type I interferon receptor 2

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Citations for Human IFN-alpha / beta  R2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Lysine catabolism reprograms tumour immunity through histone crotonylation
    Authors: Yuan, H;Wu, X;Wu, Q;Chatoff, A;Megill, E;Gao, J;Huang, T;Duan, T;Yang, K;Jin, C;Yuan, F;Wang, S;Zhao, L;Zinn, PO;Abdullah, KG;Zhao, Y;Snyder, NW;Rich, JN;
    Species: Human, Transgenic Mouse, Xenograft
    Sample Types: In Vivo, Whole Cells
    Applications: Neutralization
  2. A human iPSC-array-based GWAS identifies a virus susceptibility locus in the NDUFA4 gene and functional variants
    Authors: Y Han, L Tan, T Zhou, L Yang, L Carrau, LA Lacko, M Saeed, J Zhu, Z Zhao, BE Nilsson-Pa, FT Lira Neto, C Cahir, AM Giani, JC Chai, Y Li, X Dong, D Moroziewic, NYSCF Glob, D Paull, T Zhang, S Koo, C Tan, R Danziger, Q Ba, L Feng, Z Chen, A Zhong, GJ Wise, JZ Xiang, H Wang, RE Schwartz, BR tenOever, SA Noggle, CM Rice, Q Qi, T Evans, S Chen
    Cell Stem Cell, 2022-10-06;29(10):1475-1490.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC


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