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Human IFN-gamma DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Range
9.4 - 600 pg/mL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Interferon gamma (IFN-γ). The suggested diluent is suitable for the analysis of most cell culture supernate, serum, and plasma samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Data Examples

Human IFN-gamma ELISA Standard Curve

Human IFN-gamma ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

Long Name:
Interferon gamma
Entrez Gene IDs:
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names:
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL of Block Buffer to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human IFN-gamma DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

162 Citations: Showing 1 - 10
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  1. Novel Bi-Specific Immuno-Modulatory Tribodies Potentiate T Cell Activation and Increase Anti-Tumor Efficacy
    Authors: M Passariell, A Yoshioka, K Takahashi, SI Hashimoto, R Rapuano Le, L Manna, K Nakamura, C De Lorenzo
    International Journal of Molecular Sciences, 2022;23(7):.
    Species: Human
    Sample Types: cell culture supernatant
  2. Echinococcus multilocularis specific antibody, systemic cytokine, and chemokine levels, as well as antigen-specific cellular responses in patients with progressive, stable, and cured alveolar echinococcosis: A 10-year follow-up
    Authors: B Grüner, L Peters, A Hillenbran, P Vo beta berg, J Schweiker, EG Rollmann, LH Rodriguez, J Blumhardt, S Burkert, P Kern, C Köhler, PT Soboslay
    PloS Neglected Tropical Diseases, 2022;16(2):e0010099.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. The presence of serum anti-SARS-CoV-2 IgA appears to protect primary health care workers from COVID-19
    Authors: V Hennings, K Thörn, S Albinsson, C Lingblom, K Andersson, C Andersson, K Järbur, R Pullerits, M Idorn, SR Paludan, K Eriksson, C Wennerås
    European Journal of Immunology, 2022;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Ex vivo modelling of PD-1/PD-L1 immune checkpoint blockade under acute, chronic, and exhaustion-like conditions of T-cell stimulation
    Authors: A Roberts, L Bentley, T Tang, F Stewart, C Pallini, J Juvvanapud, GR Wallace, AJ Cooper, A Scott, D Thickett, ST Lugg, H Bancroft, B Hemming, C Ferris, G Langman, A Robinson, J Chapman, B Naidu, T Pinkney, GS Taylor, K Brock, Z Stamataki, CA Brady, SJ Curnow, J Gordon, O Qureshi, NM Barnes
    Scientific Reports, 2021;11(1):4030.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Biomarkers for Comorbidities Modulate the Activity of T-Cells in COPD
    Authors: K Jamal Jame, WJ Gallert, SD Yanik, S Panek, J Kronsbein, D Jungck, A Koch, J Knobloch
    International Journal of Molecular Sciences, 2021;22(13):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Targeting of Janus Kinases Limits Pro-Inflammatory but Also Immunosuppressive Circuits in the Crosstalk between Synovial Fibroblasts and Lymphocytes
    Authors: N Yao, T Tretter, P Kvacskay, W Merkt, N Blank, HM Lorenz, LO Tykocinski
    Biomedicines, 2021;9(10):.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Kras-driven intratumoral heterogeneity triggers infiltration of M2 polarized macrophages via the circHIPK3/PTK2 immunosuppressive circuit
    Authors: T Katopodi, S Petanidis, K Domvri, P Zarogoulid, D Anestakis, C Charalampi, D Tsavlis, C Bai, H Huang, L Freitag, W Hohenforst, D Matthaios, K Porpodis
    Scientific Reports, 2021;11(1):15455.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Monocyte-dependent co-stimulation of cytokine induction in human gammadelta T cells by TLR8 RNA ligands
    Authors: R Serrano, C Coch, C Peters, G Hartmann, D Wesch, D Kabelitz
    Scientific Reports, 2021;11(1):15231.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. A scDb-based trivalent bispecific antibody for T-cell-mediated killing of HER3-expressing cancer cells
    Authors: N Aschmoneit, S Steinlein, L Kühl, O Seifert, RE Kontermann
    Scientific Reports, 2021;11(1):13880.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Immunomodulatory mAbs as Tools to Investigate on Cis-Interaction of PD-1/PD-L1 on Tumor Cells and to Set Up Methods for Early Screening of Safe and Potent Combinatorial Treatments
    Authors: C Vetrei, M Passariell, G Froechlich, R Rapuano Le, N Zambrano, C De Lorenzo
    Cancers, 2021;13(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Beneficial Effects of Mineralocorticoid Receptor Pathway Blockade against Endothelial Inflammation Induced by SARS-CoV-2 Spike Protein
    Authors: E Jover, L Matilla, M Garaikoetx, A Fernández-, P Muntendam, F Jaisser, P Rossignol, N López-Andr
    Biomedicines, 2021;9(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Cord blood and amniotic membrane extract eye drop preparations display immune-suppressive and regenerative properties
    Authors: D Samarkanov, S Cox, D Hernandez, L Rodriguez, ML Pérez, A Madrigal, A Vilarrodon, S Querol, RP Casaroli-M
    Scientific Reports, 2021;11(1):13754.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Identification of monoclonal antibodies against human renal glomerular endothelial cells in lupus nephritis that induce endothelial interferon-alpha production
    Authors: YC Hu, IJ Tsai, HY Hsu, BL Chiang, YH Yang
    Arthritis Research & Therapy, 2021;23(1):171.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Functional antibody and T-cell immunity following SARS-CoV-2 infection, including by variants of concern, in patients with cancer: the CAPTURE study
    Authors: A Fendler, L Au, S Shepherd, F Byrne, M Cerrone, L Boos, K Rzeniewicz, W Gordon, B Shum, C Gerard, B Ward, W Xie, A Schmitt, N Joharatnam, G Cornish, M Pule, L Mekkaoui, K Ng, E Carlyle, K Edmonds, LD Rosario, S Sarker, K Lingard, M Mangwende, L Holt, H Ahmod, R Stone, C Gomes, H Flynn, A Agua-Doce, P Hobson, S Caidan, M Howell, M Wu, R Goldstone, M Crawford, L Cubitt, H Patel, M Gavrielide, E Nye, A Snijders, J MacRae, J Nicod, F Gronthoud, R Shea, C Messiou, D Cunningham, I Chau, N Starling, N Turner, L Welsh, N van As, R Jones, J Droney, S Banerjee, K Tatham, S Jhanji, M O'Brien, O Curtis, K Harrington, S Bhide, J Bazin, A Robinson, C Stephenson, T Slattery, Y Khan, Z Tippu, I Leslie, S Gennatas, A Okines, A Reid, K Young, A Furness, L Pickering, S Gandhi, S Gamblin, C Swanton, E Nicholson, S Kumar, N Yousaf, K Wilkinson, A Swerdlow, R Harvey, G Kassiotis, J Larkin, R Wilkinson, S Turajlic
    Research square, 2021;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Food Intolerance of Unknown Origin: Caused by Mucosal Inflammation? A Pilot Study
    Authors: W Dieterich, E Tietz, M Kohl, PC Konturek, T Rath, MF Neurath, Y Zopf
    Clinical and Translational Gastroenterology, 2021;12(2):e00312.
    Species: Human
    Sample Types: Tissue Homogenates
  16. Bacterial genotoxins induce T�cell senescence
    Authors: SL Mathiasen, L Gall-Mas, IS Pateras, SDP Theodorou, MRJ Namini, MB Hansen, OCB Martin, CK Vadivel, K Ntostoglou, D Butter, M Givskov, C Geisler, AN Akbar, VG Gorgoulis, T Frisan, N Ødum, T Krejsgaard
    Cell Reports, 2021;35(10):109220.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. P32-specific CAR T cells with dual antitumor and antiangiogenic therapeutic potential in gliomas
    Authors: L Rousso-Noo, I Mastandrea, S Talmor, T Waks, A Globerson, M Haugas, T Teesalu, L Alvarez-Va, Z Eshhar, D Friedmann-
    Nature Communications, 2021;12(1):3615.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. New Biomarker in Chagas Disease: Extracellular Vesicles Isolated from Peripheral Blood in Chronic Chagas Disease Patients Modulate the Human Immune Response
    Authors: RP Madeira, LM Dal'Mas Ro, P de Cássia, C Mady, BM Ianni, AC Torrecilha
    Journal of Immunology Research, 2021;2021(0):6650670.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Novel immunomodulatory properties of low dose cytarabine entrapped in a mannosylated cationic liposome
    Authors: AL Martel, NL Fraleigh, E Picard, JD Lewicky, G Pawelec, H Lee, GW Ma, L Mousavifar, R Roy, HT Le
    International journal of pharmaceutics, 2021;0(0):120849.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Severe T cell hyporeactivity in ventilated COVID-19 patients correlates with prolonged virus persistence and poor outcomes
    Authors: K Renner, T Schwittay, S Chaabane, J Gottschlin, C Müller, C Tiefenböck, JN Salewski, F Winter, S Buchtler, S Balam, MV Malferthei, M Lubnow, D Lunz, B Graf, F Hitzenbich, F Hanses, H Poeck, M Kreutz, E Orsó, R Burkhardt, T Niedermair, C Brochhause, A Gessner, B Salzberger, M Mack
    Nature Communications, 2021;12(1):3006.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Discovery of a novel anti PD-L1 X TIGIT bispecific antibody for the treatment of solid tumors
    Authors: Y Xiao, P Chen, C Luo, Z Xu, X Li, L Liu, L Zhao
    Cancer treatment and research communications, 2021;29(0):100467.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Endothelial-Derived Extracellular Vesicles Induce Cerebrovascular Dysfunction in Inflammation
    Authors: D Roig-Carle, E Willms, RD Fontijn, S Martinez-P, I Mäger, HE de Vries, M Hirst, B Sharrack, DK Male, CA Hawkes, IA Romero
    Pharmaceutics, 2021;13(9):.
    Species: Human
    Sample Types: Extracellular Vesicles
  23. Pluripotent Stem Cell-Derived Hepatocytes Inhibit T Cell Proliferation In Vitro through Tryptophan Starvation
    Authors: M Romano, R Elgueta, D McCluskey, AM Ortega-Pri, E Stolarczyk, F Dazzi, B Lucendo-Vi, J Meseguer-R, J Williams, G Fanelli, DC Hay, FM Watt, G Lombardi
    Cells, 2021;11(1):.
    Species: Human
    Sample Types: Serum
  24. alphabeta-T Cells Engineered to Express gammadelta-T Cell Receptors Can Kill Neuroblastoma Organoids Independent of MHC-I Expression
    Authors: JGM Strijker, R Pscheid, E Drent, JJF van der Ho, B Koopmans, K Ober, SR van Hooff, WM Kholosy, AM Cornel, C Coomans, A Bisso, MM van Loenen, JJ Molenaar, J Wienke
    Journal of personalized medicine, 2021;11(9):.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Reduced Interleukin-17-Expressing Cells in Cutaneous Melanoma
    Authors: A Tosi, L Nardinocch, ML Carbone, L Capriotti, E Pagani, S Mastroeni, C Fortes, F Scopelliti, C Cattani, F Passarelli, A Rosato, S D'Atri, CM Failla, A Cavani
    Biomedicines, 2021;9(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Soluble CD137 is a novel serum marker of liver cirrhosis in patients with hepatitis C and alcohol-associated disease etiology
    Authors: K Weigand, G Peschel, J Grimm, K Luu, D Schacherer, R Wiest, M Müller, H Schwarz, C Buechler
    European Journal of Immunology, 2021;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Cancer immune therapy with PD-1-dependent CD137 co-stimulation provides localized tumour killing without systemic toxicity
    Authors: Y Qiao, Y Qiu, J Ding, N Luo, H Wang, X Ling, J Sun, Z Wu, Y Wang, Y Liu, F Guo, T Sun, W Shen, M Zhang, D Wu, B Chen, W Xu, X Wang
    Nature Communications, 2021;12(1):6360.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Skin Substitute Preparation Method Induces Immunomodulatory Changes in Co-Incubated Cells through Collagen Modification
    Authors: J Holl, C Pawlukiani, J Corton Rui, D Groth, K Grubczak, HR Hady, J Dadan, J Reszec, S Czaban, C Kowalewski, M Moniuszko, A Eljaszewic
    Pharmaceutics, 2021;13(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. T-cell responses to SARS-CoV-2 in multiple sclerosis patients treated with ocrelizumab healed from COVID-19 with absent or low anti-spike antibody titers
    Authors: M Iannetta, D Landi, G Cola, V Malagnino, E Teti, D Fraboni, F Buccisano, S Grelli, L Coppola, L Campogiani, M Andreoni, GA Marfia, L Sarmati
    Multiple sclerosis and related disorders, 2021;55(0):103157.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. A Rational Designed Novel Bispecific Antibody for the Treatment of GBM
    Authors: R Sun, Y Zhou, L Han, Z Pan, J Chen, H Zong, Y Bian, H Jiang, B Zhang, J Zhu
    Biomedicines, 2021;9(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. NLRP1 acts as a negative regulator of Th17 cell programming in mice and humans with autoimmune diabetes
    Authors: FRC Costa, JA Leite, DM Rassi, JF da Silva, J Elias-Oliv, JB Guimarães, MC Foss-Freit, NOS Câmara, A Pontillo, RC Tostes, JS Silva, D Carlos
    Cell Reports, 2021;35(8):109176.
    Species: Human
    Sample Types: Serum
  32. Novel Combinations of Human Immunomodulatory mAbs Lacking Cardiotoxic Effects for Therapy of TNBC
    Authors: C Vetrei, M Passariell, G Froechlich, R Rapuano Le, E Sasso, N Zambrano, C De Lorenzo
    Cancers, 2021;14(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Ipilimumab and Its Derived EGFR Aptamer-Based Conjugate Induce Efficient NK Cell Activation against Cancer Cells
    Authors: M Passariell, S Camorani, C Vetrei, S Ricci, L Cerchia, C De Lorenzo
    Cancers (Basel), 2020;12(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Differentially Expressed Genes of Natural Killer Cells Can Distinguish Rheumatoid Arthritis Patients from Healthy Controls
    Authors: NM Elemam, MY Hachim, S Hannawi, AA Maghazachi
    Genes (Basel), 2020;11(5):.
    Species: Human
    Sample Types: Plasma
  35. Characterization of Human NK Cell-Derived Exosomes: Role of DNAM1 Receptor In Exosome-Mediated Cytotoxicity Against Tumor
    Authors: ALD Pace, N Tumino, F Besi, C Alicata, LA Conti, E Munari, E Maggi, P Vacca, L Moretta
    Cancers (Basel), 2020;12(3):.
    Species: Human
    Sample Types: Cell Culture Lysates
  36. Gaps in Study Design for Immune Parameter Research for Latent Tuberculosis Infection: A Systematic Review
    Authors: M Herrera, C Vera, Y Keynan, ZV Rueda
    J Immunol Res, 2020;2020(0):8074183.
    Species: Human
    Sample Types: Plasma
  37. Insights into P-Glycoprotein Inhibitors: New Inducers of Immunogenic Cell Death
    Authors: J Kopecka, M Godel, S Dei, R Giampietro, DC Belisario, M Akman, M Contino, E Teodori, C Riganti
    Cells, 2020;9(4):.
    Species: Human
    Sample Types: Cell Culture Supernatants
  38. Activation of Human &gamma&delta T Cells: Modulation by Toll-Like Receptor 8 Ligands and Role of Monocytes
    Authors: R Serrano, D Wesch, D Kabelitz
    Cells, 2020;9(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. &gamma&delta T Cells Kill Plasmodium falciparum in a Granzyme- and Granulysin-Dependent Mechanism during the Late Blood Stage
    Authors: MA Hernández-, K Happ, F Cattalani, A Wallimann, M Blanchard, I Fellay, B Scolari, N Lannes, S Mbagwu, B Fellay, L Filgueira, PY Mantel, M Walch
    J. Immunol., 2020;204(7):1798-1809.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Persistent elevation of intrathecal pro-inflammatory cytokines leads to multiple sclerosis-like cortical demyelination and neurodegeneration
    Authors: RE James, R Schalks, E Browne, I Eleftheria, CP Munoz, ND Mazarakis, R Reynolds
    Acta Neuropathol Commun, 2020;8(1):66.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Targeting PD-L1 in non-small cell lung cancer using CAR T cells
    Authors: M Liu, X Wang, W Li, X Yu, P Flores-Vil, ZY Xu-Monette, L Li, M Zhang, KH Young, X Ma, Y Li
    Oncogenesis, 2020;9(8):72.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. Cholesterol metabolism drives regulatory B cell IL-10 through provision of geranylgeranyl pyrophosphate
    Authors: JA Bibby, HA Purvis, T Hayday, A Chandra, K Okkenhaug, S Rosenzweig, I Aksentijev, M Wood, HJ Lachmann, C Kemper, AP Cope, E Perucha
    Nat Commun, 2020;11(1):3412.
    Species: Human
    Sample Types: Cell Culture Supernates
  43. A Cross-Sectional Study Evaluating the Effects of Resistance Exercise on Inflammation and Neurotrophic Factors in Elderly Women with Obesity
    Authors: HT Roh, SY Cho, WY So
    J Clin Med, 2020;9(3):.
    Species: Human
    Sample Types: Serum
  44. Transcriptional downregulation of MHC class I and melanoma de- differentiation in resistance to PD-1 inhibition
    Authors: JH Lee, E Shklovskay, SY Lim, MS Carlino, AM Menzies, A Stewart, B Pedersen, M Irvine, S Alavi, JYH Yang, D Strbenac, RPM Saw, JF Thompson, JS Wilmott, RA Scolyer, GV Long, RF Kefford, H Rizos
    Nat Commun, 2020;11(1):1897.
    Species: Human
    Sample Types: Cell Culture Supernates
  45. Exploring the Role of C-C Motif Chemokine Ligand-2 Single Nucleotide Polymorphism in Pulmonary Tuberculosis: A Genetic Association Study from North India
    Authors: SK Biswas, M Mittal, E Sinha, V Singh, N Arela, B Bajaj, PK Tiwari, VM Katoch, KK Mohanty
    Journal of Immunology Research, 2020;2020(0):1019639.
    Species: Human
    Sample Types: Serum
  46. Monocytes differentiated into macrophages and dendritic cells in the presence of human IFN-&lambda3 or IFN-&lambda4 show distinct phenotypes
    Authors: M De, A Bhushan, S Chinnaswam
    J Leukoc Biol, 2020;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. Lentiviral delivery of combinatorial CAR/CRISPRi circuit into human primary T cells is enhanced by TBK1/IKK? complex inhibitor BX795
    Authors: L Li, Y Gao, R Srivastava, W Wang, Q Xiong, Z Fang, A Pelayo, C Denson, A Goswami, R Harari-Ste, Z Yang, L Weng, LS Qi, FM Marincola
    Journal of Translational Medicine, 2020;18(1):363.
    Species: Human
    Sample Types: Cell Culture Supernates
  48. ADAM12 is a costimulatory molecule that determines Th1 cell fate and mediates tissue inflammation
    Authors: Y Liu, R Bockermann, M Hadi, I Safari, B Carrion, M Kveiborg, S Issazadeh-
    Cell. Mol. Immunol., 2020;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. Isolation of Two Novel Human Anti-CTLA-4 mAbs with Intriguing Biological Properties on Tumor and NK Cells
    Authors: M Passariell, C Vetrei, E Sasso, G Froechlich, C Gentile, AM D'Alise, N Zambrano, E Scarselli, A Nicosia, C De Lorenzo
    Cancers (Basel), 2020;12(8):.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. Characterization of BAY 1905254, an Immune Checkpoint Inhibitor Targeting the Immunoglobulin-Like Domain Containing Receptor 2 (ILDR2)
    Authors: J Huetter, U Gritzan, I Gutcher, WD Doecke, MV Luetke-Eve, S Golfier, HG Roider, AL Frisk, J Hunter, A Pow, A Drake, Z Levine, O Levy, M Azulay, I Barbiro, G Cojocaru, I Vaknin, B Kreft, L Roese
    Cancer Immunol Res, 2020;8(7):895-911.
    Species: Human
    Sample Types: Cell Culture Supernates
  51. An Antibody Targeting ICOS Increases Intratumoral Cytotoxic to Regulatory T-cell Ratio and Induces Tumor Regression
    Authors: RCA Sainson, AK Thotakura, M Kosmac, G Borhis, N Parveen, R Kimber, J Carvalho, SJ Henderson, KL Pryke, T Okell, S O'Leary, S Ball, C Van Krinks, L Gamand, E Taggart, EJ Pring, H Ali, H Craig, VWY Wong, Q Liang, RJ Rowlands, M Lecointre, J Campbell, I Kirby, D Melvin, V Germaschew, E Oelmann, S Quaratino, M McCourt
    Cancer Immunology Research, 2020;8(12):1568-1582.
    Species: Human
    Sample Types: Cell Culture Supernates
  52. Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability
    Authors: S Guedan, A Madar, V Casado-Med, C Shaw, A Wing, F Liu, RM Young, CH June, AD Posey
    J. Clin. Invest., 2020;130(6):3087-3097.
    Species: Human
    Sample Types: Cell Culture Supernates
  53. Interleukin-18 and Hematopoietic Recovery after Allogeneic Stem Cell Transplantation
    Authors: A Radujkovic, L Kordelas, R Bogdanov, C Müller-Tid, DW Beelen, P Dreger, T Luft
    Cancers (Basel), 2020;12(10):.
    Species: Human
    Sample Types: Serum
  54. Acute Conditioning of Antigen-Expanded CD8+ T Cells via the GSK3&beta-mTORC Axis Differentially Dictates Their Immediate and Distal Responses after Antigen Rechallenge
    Authors: P Taborska, D Stakheev, H Svobodova, Z Strizova, J Bartunkova, D Smrz
    Cancers, 2020;12(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. Clinical Response to the CD95-Ligand Inhibitor Asunercept Is Defined by a Pro-Inflammatory Serum Cytokine Profile
    Authors: A Radujkovic, T Boch, F Nolte, D Nowak, C Kunz, A Gieffers, C Müller-Tid, P Dreger, WK Hofmann, T Luft
    Cancers, 2020;12(12):.
    Species: Human
    Sample Types: Serum
  56. Aromadendrin Inhibits T Cell Activation via Regulation of Calcium Influx and NFAT Activity
    Authors: HS Lee, GS Jeong
    Molecules, 2020;25(19):.
    Species: Human
    Sample Types: Cell Culture Supernates
  57. Restricting Glycolysis Preserves T Cell Effector Functions and Augments Checkpoint Therapy
    Authors: K Renner, C Bruss, A Schnell, G Koehl, HM Becker, M Fante, AN Menevse, N Kauer, R Blazquez, L Hacker, SM Decking, T Bohn, S Faerber, K Evert, L Aigle, S Amslinger, M Landa, O Krijgsman, EA Rozeman, C Brummer, PJ Siska, K Singer, S Pektor, M Miederer, K Peter, E Gottfried, W Herr, I Marchiq, J Pouyssegur, WR Roush, S Ong, S Warren, T Pukrop, P Beckhove, SA Lang, T Bopp, CU Blank, JL Cleveland, PJ Oefner, K Dettmer, M Selby, M Kreutz
    Cell Rep, 2019;29(1):135-150.e9.
    Species: Human
    Sample Types: Cell Culture Supernates
  58. In Vitro Induction of T Helper 17 Cells by Synergistic Activation of Human Monocyte-Derived Langerhans Cell-Like Cells with Bacterial Agonists
    Authors: R Gramlich, E Aliahmadi, M Peiser
    Int J Mol Sci, 2019;20(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
  59. Crosstalk between cancer cells and tumor associated macrophages is required for mesenchymal circulating tumor cell-mediated colorectal cancer metastasis
    Authors: C Wei, C Yang, S Wang, D Shi, C Zhang, X Lin, Q Liu, R Dou, B Xiong
    Mol. Cancer, 2019;18(1):64.
    Species: Human
    Sample Types: Cell Culture Supernates
  60. Large-scale secretome analyses unveil the superior immunosuppressive phenotype of umbilical cord stromal cells as compared to other adult mesenchymal stromal cells
    Authors: A Islam, I Urbarova, JA Bruun, I Martinez-Z
    Eur Cell Mater, 2019;37(0):153-174.
    Species: Human
    Sample Types: Cell Culture Supernates
  61. Characterization of a Novel Third-Generation Anti-CD24-CAR against Ovarian Cancer
    Authors: R Klapdor, S Wang, M Morgan, T Dörk, U Hacker, P Hillemanns, H Büning, A Schambach
    Int J Mol Sci, 2019;20(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  62. A rationally designed peptide antagonist of the PD-1 signaling pathway as an immunomodulatory agent for cancer therapy
    Authors: PG Sasikumar, RK Ramachandr, S Adurthi, AA Dhudashia, S Vadlamani, K Vemula, S Vunnum, LK Satyam, DS Samiulla, K Subbarao, R Nair, R Shrimali, N Gowda, M Ramachandr
    Mol. Cancer Ther., 2019;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  63. Role of vimentin in modulating immune cell apoptosis and inflammatory responses in sepsis
    Authors: L Su, P Pan, P Yan, Y Long, X Zhou, X Wang, R Zhou, B Wen, L Xie, D Liu
    Sci Rep, 2019;9(1):5747.
    Species: Human
    Sample Types: Cell Culture Supernates
  64. MET Receptor Tyrosine Kinase Regulates the Expression of Co-Stimulatory and Co-Inhibitory Molecules in Tumor Cells and Contributes to PD-L1-Mediated Suppression of Immune Cell Function
    Authors: HK Ahn, S Kim, D Kwon, J Koh, YA Kim, K Kim, DH Chung, YK Jeon
    Int J Mol Sci, 2019;20(17):.
    Species: Human
    Sample Types: Cell Culture Supernates
  65. Programmed cell death ligand-1-mediated enhancement of hexokinase 2 expression is inversely related to T-cell effector gene expression in non-small-cell lung cancer
    Authors: S Kim, JY Jang, J Koh, D Kwon, YA Kim, JC Paeng, CY Ock, B Keam, M Kim, TM Kim, DS Heo, DH Chung, YK Jeon
    J. Exp. Clin. Cancer Res., 2019;38(1):462.
    Species: Human
    Sample Types: Media
  66. Human immune cells infiltrate the spinal cord and impair recovery after spinal cord injury in humanized mice
    Authors: RS Carpenter, RR Jiang, FH Brennan, JCE Hall, MK Gottipati, S Niewiesk, PG Popovich
    Sci Rep, 2019;9(1):19105.
    Species: Transgenic Mouse
    Sample Types: Cell Culture Supernates
  67. Replication of Genome-Wide Association Analysis Identifies New Susceptibility Loci at Long Noncoding RNA Regions for Vogt-Koyanagi-Harada Disease
    Authors: J Qi, L Du, J Deng, Y Qin, G Su, S Hou, M Lv, Q Zhang, A Kijlstra, P Yang
    Invest. Ophthalmol. Vis. Sci., 2019;60(14):4820-4829.
    Species: Human
    Sample Types: Cell Culture Supernates
  68. The human glomerular endothelial cells are potent pro-inflammatory contributors in an in vitro model of lupus nephritis
    Authors: P Dimou, RD Wright, KL Budge, A Midgley, SC Satchell, M Peak, MW Beresford
    Sci Rep, 2019;9(1):8348.
    Species: Human
    Sample Types: Cell Culture Supernates
  69. 43?kDa Glycoprotein (gp43) from Paracoccidioides brasiliensis Induced IL-17A and PGE2 Production by Human Polymorphonuclear Neutrophils: Involvement of TLR2 and TLR4
    Authors: TP Gardizani, AM Della Cole, GG Romagnoli, R Puccia, APM Serezani, ÂMV de Campos, LA Dias-Melic
    J Immunol Res, 2019;2019(0):1790908.
    Species: Human
    Sample Types: Cell Culture Supernates
  70. Clinically compliant spatial and temporal imaging of chimeric antigen receptor T-cells
    Authors: N Emami-Shah, J Foster, R Kashani, P Gazinska, C Cook, J Sosabowski, J Maher, S Papa
    Nat Commun, 2018;9(1):1081.
    Species: Human
    Sample Types: Cell Culture Supernates
  71. Utilizing ethacrynic acid and ciclopirox olamine in liver cancer
    Authors: AM Al-Dali, H Weiher, IGH Schmidt-Wo
    Oncol Lett, 2018;16(5):6854-6860.
    Species: Human
    Sample Types: Cell Culture Supernates
  72. Disabled-2 (DAB2) Overexpression Inhibits Monocyte-Derived Dendritic Cells' Function in Vogt-Koyanagi-Harada Disease
    Authors: S Yi, R Chang, J Hu, Y Qiu, Q Wang, Q Cao, G Yuan, G Su, C Zhou, Y Wang, A Kijlstra, P Yang
    Invest. Ophthalmol. Vis. Sci., 2018;59(11):4662-4669.
    Species: Human
    Sample Types: Cell Culture Supernates
  73. An Anti-Inflammatory Azaphenothiazine Inhibits Interferon ? Expression and CXCL10 Production in KERTr Cells
    Authors: L Strzadala, A Fiedorowic, E Wysokinska, E Ziolo, M Grudzie?, M Jelen, K Pluta, B Morak-Mlod, M Zimecki, W Kalas
    Molecules, 2018;23(10):.
    Species: Human
    Sample Types: Cell Culture Supernates
  74. IL-15 and IFN-? signal through the ERK pathway to inhibit HCV replication, independent of type I IFN signaling
    Authors: F Vahedi, AJ Lee, SE Collins, MV Chew, E Lusty, B Chen, A Dubey, CD Richards, JJ Feld, RS Russell, KL Mossman, AA Ashkar
    Cytokine, 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  75. Modulation of salivary cytokines in response to alcohol, tobacco and caffeine consumption: a pilot study
    Authors: CC Sheth, RM López-Pedr, MDM Jovani-San, R González-M, V Veses
    Sci Rep, 2018;8(1):16687.
    Species: Human
    Sample Types: Saliva
  76. Plasmodium falciparum Treated with Artemisinin-based Combined Therapy Exhibits Enhanced Mutation, Heightened Cortisol and TNF-? Induction
    Authors: AO Idowu, S Bhattachar, S Gradus, W Oyibo, Z George, C Black, J Igietseme, AA Azenabor
    Int J Med Sci, 2018;15(13):1449-1457.
    Species: Human
    Sample Types: Plasma
  77. TiO2 nanoparticles can selectively bind CXCL8 impacting on neutrophil chemotaxis
    Authors: J Batt, M Milward, I Chapple, M Grant, H Roberts, O Addison
    Eur Cell Mater, 2018;35(0):13-24.
  78. CLEC16A regulates splenocyte and NK cell function in part through MEK signaling
    Authors: R Pandey, M Bakay, HS Hain, B Strenkowsk, BZB Elsaqa, JD Roizen, JA Kushner, JS Orange, H Hakonarson
    PLoS ONE, 2018;13(9):e0203952.
    Species: Human
    Sample Types: Cell Culture Supernates
  79. MicroRNA-374b inhibits liver cancer progression via down regulating programmed cell death-1 expression on cytokine-induced killer cells
    Authors: F Huang, B Wang, J Zeng, S Sang, J Lei, Y Lu
    Oncol Lett, 2018;15(4):4797-4804.
    Species: Human
    Sample Types: Cell Culture Supernates
  80. Improving CART-Cell Therapy of Solid Tumors with Oncolytic Virus-Driven Production of a Bispecific T-cell Engager
    Authors: A Wing, CA Fajardo, AD Posey, C Shaw, T Da, RM Young, R Alemany, CH June, S Guedan
    Cancer Immunol Res, 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  81. Interferon priming is essential for human CD34+ cell-derived plasmacytoid dendritic cell maturation and function
    Authors: A Laustsen, RO Bak, C Krapp, L Kjær, JH Egedahl, CC Petersen, S Pillai, HQ Tang, N Uldbjerg, M Porteus, NR Roan, M Nyegaard, PW Denton, MR Jakobsen
    Nat Commun, 2018;9(1):3525.
    Species: Human
    Sample Types: Cell Culture Supernates
  82. PDGF enhances the protective effect of adipose stem cell-derived extracellular vesicles in a model of acute hindlimb ischemia
    Authors: T Lopatina, E Favaro, C Grange, M Cedrino, A Ranghino, S Occhipinti, S Fallo, F Buffolo, DA Gaykalova, MM Zanone, R Romagnoli, G Camussi
    Sci Rep, 2018;8(1):17458.
    Species: Human
    Sample Types: Cell Culture Supernates
  83. Analysis of the association between Fc receptor family gene polymorphisms and ocular Beh�et's disease in Han Chinese
    Authors: D Zhang, J Qin, L Li, G Su, G Huang, Q Cao, A Kijlstra, P Yang
    Sci Rep, 2018;8(1):4850.
    Species: Human
    Sample Types: Cell Culture Supernates
  84. Association of Long Noncoding RNAs Polymorphisms With Ankylosing Spondylitis, Vogt-Koyanagi-Harada Disease, and Behcet's Disease
    Authors: Y Yue, J Zhang, L Yang, S Liu, J Qi, Q Cao, C Zhou, Y Wang, A Kijlstra, P Yang, S Hou
    Invest. Ophthalmol. Vis. Sci., 2018;59(2):1158-1166.
    Species: Human
    Sample Types: Cell Culture Supernates
  85. Narrow Spectrum Kinase Inhibitors Demonstrate Promise for the Treatment of Dry Eye Disease and Other Ocular Inflammatory Disorders
    Authors: S Hagan, MCT Fyfe, B Ofori-Frim, K Oliver, MR Foster, S Sirohi, Y Solanke, M Doughty, A Rowley, M Taylor, S Webber, CA Walshe
    Invest. Ophthalmol. Vis. Sci., 2018;59(3):1443-1453.
    Species: Human
    Sample Types: Cell Culture Supernates
  86. An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease
    Authors: AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle
    MAbs, 2018;0(0):1-43.
    Species: Human
    Sample Types: Plasma
  87. Modifying Enzymes are Elicited by ER Stress, Generating Epitopes that are Selectively Recognized by CD4+ T Cells in Patients With Type 1 Diabetes
    Authors: ML Marre, JW McGinty, IT Chow, ME DeNicola, NW Beck, SC Kent, AC Powers, R Bottino, DM Harlan, CJ Greenbaum, WW Kwok, JD Piganelli, EA James
    Diabetes, 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  88. Anti-CD137 monoclonal antibody enhances trastuzumab-induced, natural killer cell-mediated cytotoxicity against pancreatic cancer cell lines with low human epidermal growth factor-like receptor 2 expression
    Authors: T Masu, M Atsukawa, K Nakatsuka, M Shimizu, D Miura, T Arai, H Harimoto, C Kondo, K Kaneko, S Futagami, C Kawamoto, H Takahashi, K Iwakiri
    PLoS ONE, 2018;13(12):e0200664.
    Species: Human
    Sample Types: Cell Culture Supernates
  89. TB lymphadenitis is associated with enhanced baseline and antigen - specific induction of Type 1 and Type 17 cytokines and reduced IL-1? and IL-18 at the site of infection
    Authors: GR Kathamuthu, K Moideen, D Baskaran, VV Banurekha, D Nair, G Sekar, R Sridhar, B Vidyajayan, G Gajendrara, DK Parandhama, A Srinivasan, S Babu
    Clin. Vaccine Immunol, 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  90. Suction blistering the lesional skin of vitiligo patients reveals useful biomarkers of disease activity
    Authors: JP Strassner, M Rashighi, M Ahmed Refa, JM Richmond, JE Harris
    J. Am. Acad. Dermatol, 2017;0(0):.
    Species: Human
    Sample Types: Blister Fluid
  91. Identification of an immunogenic neo-epitope encoded by mouse sarcoma using CXCR3 ligand mRNAs as sensors
    Authors: K Fujii, Y Miyahara, N Harada, D Muraoka, M Komura, R Yamaguchi, H Yagita, J Nakamura, S Sugino, S Okumura, S Imoto, S Miyano, H Shiku
    Oncoimmunology, 2017;6(5):e1306617.
    Species: Human
    Sample Types: Cell Culture Supernates
  92. Chronic Toxoplasma gondii Infection Exacerbates Secondary Polymicrobial Sepsis
    Authors: MC Souza, DM Fonseca, A Kanashiro, L Benevides, TS Medina, MS Dias, WA Andrade, G Bonfá, MAB Silva, A Gozzi, MC Borges, RT Gazzinelli, JC Alves-Filh, FQ Cunha, JS Silva
    Front Cell Infect Microbiol, 2017;7(0):116.
    Species: Human
    Sample Types: Serum
  93. Tumor-Derived Mesenchymal Stem Cells Use Distinct Mechanisms to Block the Activity of Natural Killer Cell Subsets
    Authors: S Galland, J Vuille, P Martin, I Letovanec, A Caignard, G Fregni, I Stamenkovi
    Cell Rep, 2017;20(12):2891-2905.
    Species: Human
    Sample Types: Cell Culture Supernates
  94. IL-18/IL-15/IL-12 synergy induces elevated and prolonged IFN-? production by ex vivo expanded NK cells which is not due to enhanced STAT4 activation
    Authors: E Lusty, SM Poznanski, K Kwofie, TS Mandur, DA Lee, CD Richards, AA Ashkar
    Mol. Immunol., 2017;88(0):138-147.
    Species: Human
    Sample Types: Cell Culture Supernates
  95. On the cytokine/chemokine network during Plasmodium vivax malaria: new insights to understand the disease
    Authors: NS Hojo-Souza, DB Pereira, FS de Souza, TA de Oliveir, MS Cardoso, MS Tada, GM Zanini, DC Bartholome, RT Fujiwara, LL Bueno
    Malar. J, 2017;16(1):42.
    Species: Human
    Sample Types: Plasma
  96. Mumps Virus Induces Protein-Kinase-R-Dependent Stress Granules, Partly Suppressing Type III Interferon Production
    PLoS ONE, 2016;11(8):e0161793.
    Species: Human
    Sample Types: Cell Culture Supernates
  97. DNA Sensing via TLR-9 Constitutes a Major Innate Immunity Pathway Activated during Erythema Nodosum Leprosum
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  98. Analysis of receptor tyrosine kinase genetics identifies two novel risk loci in GAS6 and PROS1 in Beh�et's disease
    Sci Rep, 2016;6(0):26662.
    Species: Human
    Sample Types: Cell Culture Supernates
  99. Bortezomib treatment sensitizes oncolytic HSV-1 treated tumors to NK cell immunotherapy
    Clin Cancer Res, 2016;0(0):.
    Species: Mouse
    Sample Types: Serum
  100. Acanthamoeba castellanii (Genotype T4) Stimulates the Production of Interleukin-10 as well as Pro-inflammatory Cytokines in THP-1 Cells, Human Peripheral Blood Mononuclear Cells and Human Monocyte-Derived Macrophages
    Infect Immun, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  101. Dual Inhibition of Rip2 and IRAK1/4 Regulates IL-1? and IL-6 in Sarcoidosis Alveolar Macrophages and Peripheral Blood Mononuclear Cells
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  102. Metabolic and adaptive immune responses induced in mice infected with tissue-dwelling nematode Trichinella zimbabwensis
    Authors: S Mukaratirw
    Open Vet J, 2016;6(3):178-184.
    Species: Mouse
    Sample Types: Serum
  103. Reduced systemic and mycobacterial antigen-stimulated concentrations of IL-1? and IL-18 in tuberculous lymphadenitis
    Cytokine, 2016;90(0):66-72.
    Species: Human
    Sample Types: Cell Culture Supernates
  104. Characterization of host and microbial determinants in individuals with latent tuberculosis infection using a human granuloma model.
    Authors: Guirado E, Mbawuike U, Keiser T, Arcos J, Azad A, Wang S, Schlesinger L
    MBio, 2015;6(1):e02537-14.
    Species: Human
    Sample Types: Cell Culture Supernates
  105. UNC-45A Is a Nonmuscle Myosin IIA Chaperone Required for NK Cell Cytotoxicity via Control of Lytic Granule Secretion.
    Authors: Iizuka Y, Cichocki F, Sieben A, Sforza F, Karim R, Coughlin K, Isaksson Vogel R, Gavioli R, McCullar V, Lenvik T, Lee M, Miller J, Bazzaro M
    J Immunol, 2015;195(10):4760-70.
    Species: Human
    Sample Types: Cell Culture Supernates
  106. Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways.
    Authors: Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D
    J Immunol, 2015;195(6):2818-28.
    Species: Human
    Sample Types: Cell Culture Supernates
  107. Interleukin-23 Receptor Gene Polymorphism May Enhance Expression of the IL-23 Receptor, IL-17, TNF-alpha and IL-6 in Behcet's Disease.
    Authors: Jiang Z, Hennein L, Tao Y, Tao L
    PLoS ONE, 2015;10(7):e0134632.
    Species: Human
    Sample Types: Cell Culture Supernates
  108. B7H6-Specific Bispecific T Cell Engagers Lead to Tumor Elimination and Host Antitumor Immunity.
    Authors: Wu M, Zhang T, Gacerez A, Coupet T, DeMars L, Sentman C
    J Immunol, 2015;194(11):5305-11.
  109. An Autocrine Cytokine/JAK/STAT-Signaling Induces Kynurenine Synthesis in Multidrug Resistant Human Cancer Cells.
    Authors: Campia I, Buondonno I, Castella B, Rolando B, Kopecka J, Gazzano E, Ghigo D, Riganti C
    PLoS ONE, 2015;10(5):e0126159.
    Species: Human
    Sample Types: Cell Culture Supernates
  110. Phenotypic profiling of CD8(+) T cells during Plasmodium vivax blood-stage infection.
    Authors: Hojo-Souza N, Pereira D, Passos L, Gazzinelli-Guimaraes P, Cardoso M, Tada M, Zanini G, Bartholomeu D, Fujiwara R, Bueno L
    BMC Infect Dis, 2015;15(0):35.
    Species: Human
    Sample Types: Plasma
  111. B7H6-specific chimeric antigen receptors lead to tumor elimination and host antitumor immunity.
    Authors: Wu M, Zhang T, DeMars L, Sentman C
    Gene Ther, 2015;22(8):675-84.
  112. IFN-gamma stimulates autophagy-mediated clearance of Burkholderia cenocepacia in human cystic fibrosis macrophages.
    Authors: Assani K, Tazi M, Amer A, Kopp B
    PLoS ONE, 2014;9(5):e96681.
    Species: Human
    Sample Types: Cell Culture Supernates
  113. The aryl hydrocarbon receptor is functionally upregulated early in the course ofvhuman T-cell activation.
    Authors: Prigent L, Robineau M, Jouneau S, Morzadec C, Louarn L, Vernhet L, Fardel O, Sparfel L
    Eur J Immunol, 2014;44(5):1330-40.
    Species: Human
    Sample Types: Cell Culture Supernates
  114. A functional variant of PTPN22 confers risk for Vogt-Koyanagi-Harada syndrome but not for ankylosing spondylitis.
    Authors: Zhang Q, Qi J, Hou S, DU L, Yu H, Cao Q, Zhou Y, Liao D, Kijlstra A, Yang P
    PLoS ONE, 2014;9(5):e96943.
    Species: Human
    Sample Types: Cell Culture Supernates
  115. Human CD4+ T cell responses to the dog major allergen Can f 1 and its human homologue tear lipocalin resemble each other.
    Authors: Liukko A, Kinnunen T, Rytkonen-Nissinen M, Kailaanmaki A, Randell J, Maillere B, Virtanen T
    PLoS ONE, 2014;9(5):e98461.
  116. TNF-alpha blockade induces IL-10 expression in human CD4+ T cells.
    Authors: Evans H, Roostalu U, Walter G, Gullick N, Frederiksen K, Roberts C, Sumner J, Baeten D, Gerwien J, Cope A, Geissmann F, Kirkham B, Taams L
    Nat Commun, 2014;5(0):3199.
    Species: Human
    Sample Types: Cell Culture Supernates
  117. The role of interleukin-1 receptor-associated kinases in Vogt-Koyanagi-Harada disease.
    Authors: Sun M, Yang P, DU L, Yang Y, Ye J
    PLoS ONE, 2014;9(4):e93214.
    Species: Human
    Sample Types: Cell Culture Supernates
  118. Activin A as a mediator of NK-dendritic cell functional interactions.
    Authors: Seeger P, Bosisio D, Parolini S, Badolato R, Gismondi A, Santoni A, Sozzani S
    J Immunol, 2014;192(3):1241-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  119. Interferon-gamma production by human neutrophils upon stimulation by IL-12, IL-15 and IL-18 and challenge with Paracoccidioides brasiliensis.
    Authors: Rodrigues D, Fernandes R, Balderramas H, Penitenti M, Bachiega T, Calvi S, Dias-Melicio L, Ikoma M, Soares A
    Cytokine, 2014;69(1):102-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  120. Immunoepidemiological profiling of onchocerciasis patients reveals associations with microfilaria loads and ivermectin intake on both individual and community levels.
    Authors: Arndts, Kathrin, Specht, Sabine, Debrah, Alexande, Tamarozzi, Francesc, Klarmann Schulz, Ute, Mand, Sabine, Batsa, Linda, Kwarteng, Alexande, Taylor, Mark, Adjei, Ohene, Martin, Coralie, Layland, Laura E, Hoerauf, Achim
    PLoS Negl Trop Dis, 2014;8(2):e2679.
    Species: Human
    Sample Types: Cell Culture Supernates
  121. Specific targeting of a naturally presented osteosarcoma antigen, papillomavirus binding factor peptide, using an artificial monoclonal antibody.
    Authors: Tsukahara T, Emori M, Murata K, Hirano T, Muroi N, Kyono M, Toji S, Watanabe K, Torigoe T, Kochin V, Asanuma H, Matsumiya H, Yamashita K, Himi T, Ichimiya S, Wada T, Yamashita T, Hasegawa T, Sato N
    J Biol Chem, 2014;289(32):22035-47.
    Species: Human
    Sample Types: Cell Culture Supernates
  122. T-cell engager-armed oncolytic vaccinia virus significantly enhances antitumor therapy.
    Authors: Yu F, Wang X, Guo Z, Bartlett D, Gottschalk S, Song X
    Mol Ther, 2014;22(1):102-11.
    Species: Human
    Sample Types: Cell Culture Supernates
  123. Natural killer cell-mediated shedding of ULBP2.
    Authors: Wang, Ruipeng, Sun, Peter D
    PLoS ONE, 2014;9(3):e91133.
    Species: Human
    Sample Types: Cell Culture Supernates
  124. Engagement of SLAMF2/CD48 prolongs the time frame of effective T cell activation by supporting mature dendritic cell survival.
    Authors: Kis-Toth K, Tsokos G
    J Immunol, 2014;192(9):4436-42.
    Species: Human
    Sample Types: Cell Culture Supernates
  125. CD137 ligand signalling induces differentiation of primary acute myeloid leukaemia cells.
    Authors: Cheng K, Wong S, Linn Y, Ho L, Chng W, Schwarz H
    Br J Haematol, 2014;165(1):134-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  126. CD137L-stimulated dendritic cells are more potent than conventional dendritic cells at eliciting cytotoxic T-cell responses.
    Authors: Harfuddin Z, Kwajah S, Chong Nyi Sim A, Macary P, Schwarz H
    Oncoimmunology, 2013;2(11):e26859.
    Species: Human
    Sample Types: Cell Culture Supernates
  127. Fcgamma receptor-induced soluble vascular endothelial growth factor receptor-1 (VEGFR-1) production inhibits angiogenesis and enhances efficacy of anti-tumor antibodies.
    Authors: Justiniano S, Elavazhagan S, Fatehchand K, Shah P, Mehta P, Roda J, Mo X, Cheney C, Hertlein E, Eubank T, Marsh C, Muthusamy N, Butchar J, Byrd J, Tridandapani S
    J Biol Chem, 2013;288(37):26800-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  128. TLR3 impairment in human newborns.
    Authors: Slavica L, Nordstrom I, Karlsson M, Valadi H, Kacerovsky M, Jacobsson B, Eriksson K
    J Leukoc Biol, 2013;94(5):1003-11.
    Species: Human
    Sample Types: Cell Culture Supernates
  129. Elevated adaptive immune responses are associated with latent infections of Wuchereria bancrofti.
    Authors: Arndts K, Deininger S, Specht S, Klarmann U, Mand S, Adjobimey T, Debrah AY, Batsa L, Kwarteng A, Epp C, Taylor M, Adjei O, Layland LE, Hoerauf A
    PLoS Negl Trop Dis, 2012;6(4):e1611.
    Species: Human
    Sample Types: Cell Culture Supernates
  130. IL-38 binds to the IL-36 receptor and has biological effects on immune cells similar to IL-36 receptor antagonist.
    Authors: van de Veerdonk FL, Stoeckman AK, Wu G, Boeckermann AN, Azam T, Netea MG, Joosten LA, Van der Meer JW, Hao R, Kalabokis V, Dinarello CA
    Proc. Natl. Acad. Sci. U.S.A., 2012;109(8):3001-5.
    Species: Human
    Sample Types: Cell Culture Supernates
  131. NKG2D receptor regulates human effector T-cell cytokine production.
    Authors: Barber A, Sentman CL
    Blood, 2011;117(24):6571-81.
    Species: Human
    Sample Types: Cell Culture Supernates
  132. Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma.
    Authors: Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, Escudier B, Azzarone B, Caignard A
    Oncogene, 2011;30(23):2622-32.
    Species: Human
    Sample Types: Cell Culture Supernates
  133. Elevated serum osteopontin levels and genetic polymorphisms of osteopontin are associated with Vogt-Koyanagi-Harada disease.
    Authors: Chu M, Yang P, Hu R, Hou S, Li F, Chen Y, Kijlstra A
    Invest. Ophthalmol. Vis. Sci., 2011;52(10):7084-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  134. Serum levels of Th17-related cytokines in Behcet disease patients after cataract surgery.
    Authors: Jiang S, Liu X, Luo L, Qu B, Huang X, Lin Y, Ye S, Liu Y
    Mol. Vis., 2011;17(0):1425-30.
    Species: Human
    Sample Types: Serum
  135. Immunoregulation of autocrine prolactin: suppressing the expression of costimulatory molecules and cytokines in T lymphocytes by prolactin receptor knockdown.
    Authors: Xu D, Lin L, Lin X
    Cell. Immunol., 2010;263(1):71-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  136. The effects of vaccine timing on the efficacy of an acute eccentric exercise intervention on the immune response to an influenza vaccine in young adults.
    Authors: Campbell JP, Edwards KM, Ring C
    Brain Behav. Immun., 2010;24(2):236-42.
    Species: Human
    Sample Types: Plasma
  137. IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
    Authors: Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Immunol., 2010;184(9):4880-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  138. Interferon-beta treatment in multiple sclerosis attenuates inflammatory gene expression through inducible activity of the phosphatase SHP-1.
    Authors: Christophi GP, Panos M, Hudson CA, Tsikkou C, Mihai C, Mejico LJ, Jubelt B, Massa PT
    Clin. Immunol., 2009;133(1):27-44.
    Species: Human
    Sample Types: Plasma
  139. Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma.
    Authors: Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A
    J. Allergy Clin. Immunol., 2009;123(1):239-48.
    Species: Human
    Sample Types: Cell Culture Supernates
  140. Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells.
    Authors: Cerboni C, Ardolino M, Santoni A, Zingoni A
    Blood, 2009;113(13):2955-64.
    Species: Human
    Sample Types: Cell Culture Supernates
  141. TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
    Authors: Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M
    Eur. J. Immunol., 2009;39(5):1221-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  142. Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
    Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
    Circulation, 2009;119(10):1424-32.
    Species: Human
    Sample Types: Plasma
  143. Adenosine receptors in regulation of dendritic cell differentiation and function.
    Authors: Novitskiy SV, Ryzhov S, Zaynagetdinov R, Goldstein AE, Huang Y, Tikhomirov OY, Blackburn MR, Biaggioni I, Carbone DP, Feoktistov I, Dikov MM
    Blood, 2008;112(5):1822-31.
    Species: Human
    Sample Types: Cell Culture Supernates
  144. Diminished frequency and function of CD4+CD25high regulatory T cells associated with active uveitis in Vogt-Koyanagi-Harada syndrome.
    Authors: Chen L, Yang P, Zhou H, He H, Ren X, Chi W, Wang L, Kijlstra A
    Invest. Ophthalmol. Vis. Sci., 2008;49(8):3475-82.
    Species: Human
    Sample Types: Cell Culture Supernates
  145. Fungal proteases induce Th2 polarization through limited dendritic cell maturation and reduced production of IL-12.
    Authors: Lamhamedi-Cherradi SE, Martin RE, Ito T, Kheradmand F, Corry DB, Liu YJ, Moyle M
    J. Immunol., 2008;180(9):6000-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  146. Helminth infections associated with multiple sclerosis induce regulatory B cells.
    Authors: Correale J, Farez M, Razzitte G
    Ann. Neurol., 2008;64(2):187-99.
    Species: Human
    Sample Types: Cell Culture Supernates
  147. alpha1beta1 integrin and interleukin-7 receptor up-regulate the expression of RANKL in human T cells and enhance their osteoclastogenic function.
    Authors: Gendron S, Boisvert M, Chetoui N, Aoudjit F
    Immunology, 2008;125(0):359.
    Species: Human
    Sample Types: Cell Culture Supernates
  148. Chimeric NKG2D receptor-bearing T cells as immunotherapy for ovarian cancer.
    Authors: Barber A, Zhang T, DeMars LR, Conejo-Garcia J, Roby KF, Sentman CL
    Cancer Res., 2007;67(10):5003-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  149. Neuroimmune crosstalk in asthma: dual role of the neurotrophin receptor p75NTR.
    Authors: Nassenstein C, Kammertoens T, Veres TZ, Uckert W, Spies E, Fuchs B, Krug N, Braun A
    J. Allergy Clin. Immunol., 2007;120(5):1089-96.
    Species: Mouse
    Sample Types: BALF
  150. The differential effect of Eriobotrya japonica hydrophilic leaf extract on cytokines production and modulation.
    Authors: Matalka KZ, Ali D, Khawad AE, Qa'dan F
    Cytokine, 2007;40(3):235-40.
    Species: Human
    Sample Types: Cell Culture Supernates
  151. IL-24 modulates IFN-gamma expression in patients with tuberculosis.
    Authors: Wu B, Huang C, Kato-Maeda M, Hopewell PC, Daley CL, Krensky AM, Clayberger C
    Immunol. Lett., 2007;117(1):57-62.
    Species: Human
    Sample Types: Cell Culture Supernates
  152. The direct effects of Toll-like receptor ligands on human NK cell cytokine production and cytotoxicity.
    Authors: Lauzon NM, Mian F, MacKenzie R, Ashkar AA
    Cell. Immunol., 2006;241(2):102-12.
    Species: Human
    Sample Types: Cell Culture Supernates
  153. An investigation of auto-reactivity after head injury.
    Authors: Cox AL, Coles AJ, Nortje J, Bradley PG, Chatfield DA, Thompson SJ, Menon DK
    J. Neuroimmunol., 2006;174(1):180-6.
    Species: Human
    Sample Types: Cell Culture Supernates
  154. A combination of E. coli DNA fragments and modified lipopolysaccharides as a cancer immunotherapy.
    Authors: Cho YJ, Ahn BY, Lee NG, Lee DH, Kim DS
    Vaccine, 2006;24(31):5862-71.
    Species: Human
    Sample Types: Cell Culture Supernates
  155. The Toll-like receptor-2/6 agonist macrophage-activating lipopeptide-2 cooperates with IFN-gamma to reverse the Th2 skew in an in vitro allergy model.
    Authors: Weigt H, Muhlradt PF, Larbig M, Krug N, Braun A
    J. Immunol., 2004;172(10):6080-6.
    Species: Human
    Sample Types: Cell Culture Supernates
  156. Activation of resting human primary T cells with chimeric receptors: costimulation from CD28, inducible costimulator, CD134, and CD137 in series with signals from the TCR zeta chain.
    Authors: Finney HM, Akbar AN, Lawson AD
    J. Immunol., 2004;172(1):104-13.
    Species: Human
    Sample Types: Cell Culture Supernates
  157. Immune responses in normal Indian langur monkeys (Presbytis entellus)--a primate model for visceral leishmaniasis.
    Authors: Misra A, Dube A, Naik S
    J. Med. Primatol., 2004;33(2):65-9.
    Species: Primate - Presbytis entellus (Indian langur)
    Sample Types: Cell Culture Supernates
  158. Association of type 2 cytokines with hepatic fibrosis in human Schistosoma mansoni infection.
    Authors: de Jesus AR, Magalhaes A, Miranda DG, Miranda RG, Araujo MI, de Jesus AA, Silva A, Santana LB, Pearce E, Carvalho EM
    Infect. Immun., 2004;72(6):3391-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  159. IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
    Authors: Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E
    J. Immunol., 2004;173(2):1463-76.
    Species: Human
    Sample Types: Cell Culture Supernates
  160. Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis.
    Authors: Groh V, Bruhl A, El-Gabalawy H, Nelson JL, Spies T
    Proc. Natl. Acad. Sci. U.S.A., 2003;100(16):9452-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  161. Toxoplasma gondii induces granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor secretion by human fibroblasts: implications for neutrophil apoptosis.
    Authors: Channon JY, Miselis KA, Minns LA, Dutta C, Kasper LH
    Infect. Immun., 2002;70(11):6048-57.
    Species: Human
    Sample Types: Cell Culture Supernates
  162. Natural killer cell-produced IFN-gamma and TNF-alpha induce target cell cytolysis through up-regulation of ICAM-1.
    Authors: Wang R, Jaw JJ, Jaw J, Stutzman N, Stutzman NC, Zou Z, Sun PD
    J Leukoc Biol, 0;91(2):299-309.
    Species: Human
    Sample Types: Cell Culture Supernates

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Reviews for Human IFN-gamma DuoSet ELISA

Average Rating: 4.6 (Based on 10 Reviews)

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Human IFN-gamma DuoSet ELISA
By James Williams on 10/13/2021
Sample Tested: Cell culture supernatant

Human IFN-gamma DuoSet ELISA
By Anonymous on 09/28/2021
Sample Tested: Cell culture supernatant

Human IFN-gamma DuoSet ELISA
By Robert Berahovich on 09/29/2020
Sample Tested: Tissue Culture Media

Detection of IFN-g produced by BCMA-specific CAR-T cells in response to BCMA-negative and BCMA-positive hematopoietic cell lines.


Human IFN-gamma DuoSet ELISA
By Liang Lin on 08/26/2020
Sample Tested: T cells

Human IFN-gamma DuoSet ELISA
By Tahmineh Safaie on 06/16/2020
Sample Tested: Blood mononuclear cells (PBMCs)

Human IFN-gamma DuoSet ELISA
By Anonymous on 12/12/2019
Sample Tested: cell supernatant

Human IFN-gamma DuoSet ELISA
By Anonymous on 07/02/2019
Sample Tested: EDTA Plasma

Human IFN-gamma DuoSet ELISA
By Meng-Wei Ko on 12/17/2018
Sample Tested: Cell culture supernatant

Human IFN-gamma DuoSet ELISA
By Marry Tahani on 11/16/2018
Sample Tested: Cell culture supernatant,Natural killer cells

Nice linear curve. Good detection rate for the tissue culture supernatant


Human IFN-gamma DuoSet ELISA
By BOCHATON Thomas on 11/21/2017
Sample Tested: Serum

We used human serum at a dilution of 1:1 with an good technical result. However, IFNg was not detectable in human serum in our conditions.