Human IFN-gamma Quantikine HS ELISA Kit

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HSDIF0
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Human IFN-gamma  HS Quantikine ELISA Standard Curve
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Human IFN-gamma Quantikine HS ELISA Kit Summary

Assay Length
4.0 hours
Sample Type & Volume Required Per Well
Serum (100 uL), Plasma (100 uL)
Sensitivity
0.173 pg/mL
Assay Range
0.5 - 30 pg/mL (Serum, Plasma)
Specificity
Natural and recombinant human IFN-gamma.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® HS Human IFN-gamma Immunoassay is a 4.0 hour solid-phase ELISA designed to measure human IFN-gamma in serum and plasma. It contains HEK293-expressed recombinant human IFN-gamma and antibodies raised against the recombinant protein. Results obtained using natural human IFN-gamma showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-gamma.

Precision

Intra-Assay Precision (Precision within an assay) <div>Three samples of known concentration were tested twenty times on one plate to assess&nbsp;intra-assay precision.</div>
Inter-Assay Precision (Precision between assays) <div>Three samples of known concentration were tested in twenty separate assays to assess&nbsp;inter-assay precision. Assays were performed by at least three technicians.</div>

Serum, Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 1.83 6.1 12.8 2.15 6.33 12.4
Standard Deviation 0.084 0.187 0.253 0.21 0.421 0.898
CV% 4.6 3.1 2 9.8 6.7 7.2

Recovery

The recovery of human IFN-gamma spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 99 90-108
Heparin Plasma (n=4) 99 92-109
Serum (n=4) 97 85-109

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human IFN-gamma were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human IFN-gamma  HS Linearity Curve Quantikine ELISA

Data Examples

Human IFN-gamma HS Quantikine ELISA Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

Long Name:
Interferon gamma
Entrez Gene IDs:
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names:
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

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