Human IFN-gamma Quantikine HS ELISA Kit
Human IFN-gamma Quantikine HS ELISA Kit Summary
Product Summary
Precision
Serum, Plasma, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 1.83 | 6.1 | 12.8 | 2.15 | 6.33 | 12.4 |
| Standard Deviation | 0.084 | 0.187 | 0.253 | 0.21 | 0.421 | 0.898 |
| CV% | 4.6 | 3.1 | 2 | 9.8 | 6.7 | 7.2 |
Recovery
The recovery of human IFN-gamma spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 99 | 90-108 |
| Heparin Plasma (n=4) | 99 | 92-109 |
| Serum (n=4) | 97 | 85-109 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: IFN-gamma
IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.
Citations for Human IFN-gamma Quantikine HS ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Interferon and interferon-induced cytokines as markers of impending clinical progression in ANA+ individuals without a systemic autoimmune rheumatic disease diagnosis
Authors: ST Kim, C Muñoz-Graj, SE Dunn, R Schneider, SR Johnson, Z Touma, Z Ahmad, D Bonilla, EG Atenafu, LT Hiraki, A Bookman, J Wither
Arthritis Research & Therapy, 2023-02-10;25(1):21.
Species: Human
Sample Types: Serum
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The prognostic significance of serum interferon-gamma (IFN-gamma) in hormonally dependent breast cancer
Authors: N Todorovi?-, J Milovanovi, J Greenman, M Radulovic
Cytokine, 2022-02-23;152(0):155836.
Species: Human
Sample Types: Serum
FAQs
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Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?
A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.
ELISA Controls
Reviews for Human IFN-gamma Quantikine HS ELISA Kit
Average Rating: 5 (Based on 2 Reviews)
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