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Human IFN-gamma Quantikine HS ELISA Kit

R&D Systems | Catalog # HSDIF0

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Key Product Details

Assay Length

4.0 hours

Sample Type & Volume Required Per Well

Serum (100 µL), EDTA Plasma (0 Not Assigned), Heparin Plasma (100 µL)

Sensitivity

0.173 pg/mL

Assay Range

0.469-30 pg/mL (Serum, Plasma, EDTA Plasma, Heparin Plasma)
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Product Summary for Human IFN-gamma Quantikine HS ELISA Kit

The Quantikine® HS Human IFN-gamma Immunoassay is a 4.0 hour solid-phase ELISA designed to measure human IFN-gamma in serum and plasma. It contains HEK293-expressed recombinant human IFN-gamma and antibodies raised against the recombinant protein. Results obtained using natural human IFN-gamma showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-gamma.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450 or 490nm (TMB or AP Substrate)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human IFN-gamma.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians.

EDTA Plasma, Heparin Plasma, Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 1.83 6.10 12.8 2.15 6.33 12.4
Standard Deviation 0.084 0.187 0.253 0.210 0.421 0.898
CV% 4.6 3.1 2.0 9.8 6.7 7.2

Recovery for Human IFN-gamma Quantikine HS ELISA Kit

The recovery of human IFN-gamma spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 99 90-108
Heparin Plasma (n=4) 99 92-109
Serum (n=4) 97 85-109

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human IFN-gamma were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Human IFN-gamma HS Linearity Curve Quantikine ELISA

Scientific Data Images for Human IFN-gamma Quantikine HS ELISA Kit

Human IFN-gamma HS Quantikine ELISA Standard Curve

Human IFN-gamma HS Quantikine ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

Long Name

Interferon gamma

Alternate Names

IFG, IFI, IFNG, IFNgamma

Entrez Gene IDs

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Gene Symbol

IFNG

Additional IFN-gamma Products

Product Documents for Human IFN-gamma Quantikine HS ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IFN-gamma Quantikine HS ELISA Kit

For research use only

Citations for Human IFN-gamma Quantikine HS ELISA Kit

Customer Reviews for Human IFN-gamma Quantikine HS ELISA Kit (2)

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  • Human IFN-gamma Quantikine HS ELISA Kit
    Name: Anonymous
    Sample Tested: Serum-free Cell Culture Supernates
    Verified Customer | Posted 12/03/2021
  • Human IFN-gamma Quantikine HS ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 10/21/2021

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FAQs for Human IFN-gamma Quantikine HS ELISA Kit

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  • Q: Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

    A: A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

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