Human IGF-I/IGF-1 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY291
DY291-05
Ancillary Products Available
Human IGF-I ELISA Standard Curve
1 Image
Product Details
Procedure
Citations (21)
FAQs
Supplemental Products
Reviews (4)

Human IGF-I/IGF-1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IGF-I. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 3 (5 plates): (Catalog # DY009) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 3.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY004), or 5% Tween 20 in PBS, 7.2-7.4, 0.2 μm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

 

Data Example

Human IGF-I ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGF-I/IGF-1

Insulin-like growth factor 1 (IGF-1, also known as somatomedin C) is a 7.6 kDa, 70 amino acid (aa) polypeptide with three internal disulfide bonds. The sequence of human IGF-1 is identical to that of bovine and porcine IGF-1, and is 70% identical to human IGF2. IGF-1 is a single-chain molecule with about 50% identity to the sequences of the A- and B-chains of human insulin. 


IGF-1 is a growth hormone that plays a role in a variety of biological events. It is produced primarily by hepatocytes, serving an endocrine function. It is also produced by many other cells, where it may act in an autocrine or paracrine manner. It binds two tyrosine kinase receptors, the IGF-1 receptor (IGF1R) and the insulin receptor, to initiate downstream events like the AKT and PI3K signal transduction pathways. This triggers cell proliferation and protects cells from apoptosis. IGF-1 also interacts with seven IGF-binding proteins (IGFBP-1 through IGFBP7), influencing IGF-1 binding to IGF1R and increasing IGF-1 half-life to closely regulate IGF-1 signaling. For example, IGFBP-3 binds over 90% of the total IGF in serum in a complex of IGF, IGFBP, and an acid-labile subunit. This ternary complex greatly stabilizes IGF in the circulation, changing the half-life from minutes to hours. Proteases also facilitate IGF-1R binding by cleaving IGFBPs to modify their affinity for IGF or completely eliminate the IGFBP. The interactions of IGF, IGFBP, IGFBP proteases, and IGF receptors are referred to as the IGF axis. 

The IGF axis affects many primary physiological and pathological processes, including development, growth, metabolic regulation, tumorigenesis, atherosclerosis, and angiogenesis. Its ability to inhibit apoptosis and stimulate cell growth and proliferation plays a significant role in prenatal development, growth to adulthood, and metabolic control. Serum levels of IGF-1 have been reported to increase from birth to puberty, followed by a slow decline through adulthood. IGF-1 also induces amino acid uptake, protein synthesis, and glucose utilization. In the brain, IGF-1 acts as a neurotrophic factor to promote neurogenesis and neuronal survival. Exercise increases levels of IGF-1 in blood serum, indicating it plays a role as a key mediator of exercise-induced neurogenesis.

Long Name:
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Entrez Gene IDs:
3479 (Human); 16000 (Mouse); 24482 (Rat)
Alternate Names:
IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C

Assay Procedure

GENERAL ELISA PROTOCOL


Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL of Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human IGF-I/IGF-1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Moderate- to high intensity aerobic and resistance exercise reduces peripheral blood regulatory cell populations in older adults with rheumatoid arthritis
    Authors: SEM Andersson, E Lange, D Kucharski, S Svedlund, K Önnheim, M Bergquist, E Josefsson, JM Lord, IL Mårtensson, K Mannerkorp, I Gjertsson
    Immun Ageing, 2020;17(0):12.
    Species: Human
    Sample Types: Plasma
  2. Using a cultural dance program to increase sustainable physical activity for breast cancer survivors-A pilot study
    Authors: LWM Loo, K Nishibun, L Welsh, T Makolo, CD Chong, I Pagano, H Yu, EO Bantum
    Complement Ther Med, 2019;47(0):102197.
    Species: Human
    Sample Types: Plasma
  3. Changes on the Structural Architecture and Growth Factor Release, and Degradation in Equine Platelet-Rich Fibrin Clots Cultured Over Time
    Authors: RF Jiménez-Ar, JU Carmona, M Prades
    J. Equine Vet. Sci., 2019;82(0):102789.
    Species: Equine
    Sample Types: Serum
  4. Plasma Rich in Growth Factors (PRGF) Disrupt the Blood-Brain Barrier Integrity and Elevate Amyloid Pathology in the Brains of 5XFAD Mice
    Authors: QV Duong, ML Kintzing, WE Kintzing, IM Abdallah, AD Brannen, A Kaddoumi
    Int J Mol Sci, 2019;20(6):.
    Species: Human
    Sample Types: Plasma
  5. Large-scale secretome analyses unveil the superior immunosuppressive phenotype of umbilical cord stromal cells as compared to other adult mesenchymal stromal cells
    Authors: A Islam, I Urbarova, JA Bruun, I Martinez-Z
    Eur Cell Mater, 2019;37(0):153-174.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Comparative Analysis of Different Platelet Lysates and Platelet Rich Preparations to Stimulate Tendon Cell Biology: An In Vitro Study
    Authors: F Klatte-Sch, T Schmidt, M Uckert, S Scheffler, U Kalus, M Rojewski, H Schrezenme, A Pruss, B Wildemann
    Int J Mol Sci, 2018;19(1):.
    Species: Human
    Sample Types: Plasma
  7. Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton Jelly mesenchymal stromal cells
    Authors: MS Chen, TJ Wang, HC Lin, B Thierry
    New biotechnology, 2018;0(0):.
    Species: Human
    Sample Types: Platelet Lysates
  8. Tumor-associated B-cells induce tumor heterogeneity and therapy resistance
    Authors: R Somasundar, G Zhang, M Fukunaga-K, M Perego, C Krepler, X Xu, C Wagner, D Hristova, J Zhang, T Tian, Z Wei, Q Liu, K Garg, J Griss, R Hards, M Maurer, C Hafner, M Mayerhöfer, G Karanikas, A Jalili, V Bauer-Pohl, F Weihsengru, K Rappersber, J Koller, R Lang, C Hudgens, G Chen, M Tetzlaff, L Wu, DT Frederick, RA Scolyer, GV Long, M Damle, C Ellingswor, L Grinman, H Choi, BJ Gavin, M Dunagin, A Raj, N Scholler, L Gross, M Beqiri, K Bennett, I Watson, H Schaider, MA Davies, J Wargo, BJ Czerniecki, L Schuchter, D Herlyn, K Flaherty, M Herlyn, SN Wagner
    Nat Commun, 2017;8(1):607.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. The Effects of Taekwondo Training on Peripheral Neuroplasticity-Related Growth Factors, Cerebral Blood Flow Velocity, and Cognitive Functions in Healthy Children: A Randomized Controlled Trial
    Authors: SY Cho, WY So, HT Roh
    Int J Environ Res Public Health, 2017;14(5):.
    Species: Human
    Sample Types: Serum
  10. Antecedents and correlates of blood concentrations of neurotrophic growth factors in very preterm newborns
    Authors: A Leviton, EN Allred, H Yamamoto, RN Fichorova, K Kuban, TM O'Shea, O Dammann, ELGAN Stud
    Cytokine, 2017;0(0):.
    Species: Human
    Sample Types: Complex Sample Type
  11. Higher IGFBP-1 to IGF-1 serum ratio predicts unfavourable survival in patients with nasopharyngeal carcinoma
    Authors: X Feng, J Lin, S Xing, W Liu, G Zhang
    BMC Cancer, 2017;17(1):90.
    Species: Human
    Sample Types: Serum
  12. MODELING STROMA-INDUCED DRUG RESISTANCE IN A TISSUE-ENGINEERED TUMOR MODEL OF EWING SARCOMA
    Authors: Marco Santoro
    Tissue Eng Part A, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. A Collagen Cardiac Patch Incorporating Alginate Microparticles Permits the Controlled Release of HGF and IGF-1 to Enhance Cardiac Stem Cell Migration and Proliferation
    Authors: Hugh S O'Neill
    J Tissue Eng Regen Med, 2016;0(0):.
  14. Transplantation of human adipose tissue-derived stem cells delays clinical onset and prolongs life span in ALS mouse model.
    Authors: Kim, Kwang S, Lee, Hong J, An, Jin, Kim, Yun B, Ra, Jung Cha, Lim, Inja, Kim, Seung U
    Cell Transplant, 2014;23(12):1585-97.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Insulin-like growth factor 1 and 2 (IGF1, IGF2) expression in human microglia: differential regulation by inflammatory mediators.
    Authors: Suh H, Zhao M, Derico L, Choi N, Lee S
    J Neuroinflammation, 2013;10(0):37.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Sustained production of a soluble IGF-I receptor by gutless adenovirus-transduced host cells protects from tumor growth in the liver.
    Authors: Wang N, Lu Y, Pinard M, Pilotte A, Gilbert R, Massie B, Brodt P
    Cancer Gene Ther, 2013;20(4):229-36.
    Species: Human
    Sample Types: Plasma
  17. Improvement in disability after alemtuzumab treatment of multiple sclerosis is associated with neuroprotective autoimmunity.
    Authors: Jones JL, Anderson JM, Phuah CL, Fox EJ, Selmaj K, Margolin D, Lake SL, Palmer J, Thompson SJ, Wilkins A, Webber DJ, Compston DA, Coles AJ
    Brain, 2010;133(0):2232-47.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Insulin-like growth factor-I receptor blockade by a specific tyrosine kinase inhibitor for human gastrointestinal carcinomas.
    Authors: Piao W, Wang Y, Adachi Y, Yamamoto H, Li R, Imsumran A, Li H, Maehata T, Ii M, Arimura Y, Lee CT, Shinomura Y, Carbone DP, Imai K
    Mol. Cancer Ther., 2008;7(6):1483-93.
    Species: Human
    Sample Types: Tissue Homogenates
  19. Interaction between interferon gamma and insulin-like growth factor-1 in hippocampus impacts on the ability of rats to sustain long-term potentiation.
    Authors: Maher FO, Clarke RM, Kelly A, Nally RE, Lynch MA
    J. Neurochem., 2006;96(6):1560-71.
    Species: Rat
    Sample Types: Tissue Homogenates
  20. Genetic regulation of the variation of circulating insulin-like growth factors and leptin in human pedigrees.
    Authors: Pantsulaia Ia, Pantsulaia I, Trofimov S, Kobyliansky E, Livshits G
    Metab. Clin. Exp., 2005;54(7):975-81.
    Species: Human
    Sample Types: Plasma
  21. Salivary and serum insulin-like growth factor (IGF-1) assays in anorexic patients.
    Authors: Paszynska E, Dmitrzak-Weglarz M, Slopien A, Tyszkiewicz-Nwafor M, Rajewski A
    World J Biol Psychiatry, 0;17(8):615-621.
    Species: Human
    Sample Types: Saliva

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Reviews for Human IGF-I/IGF-1 DuoSet ELISA

Average Rating: 4.8 (Based on 4 Reviews)

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Human IGF-I/IGF-1 DuoSet ELISA
By Anonymous on 11/12/2020
Application: Sample Tested: EDTA Plasma

Easy to use, the kit performs well on EDTA plasma when diluted 1:2 with Reagent diluent


Human IGF-I/IGF-1 DuoSet ELISA
By Anonymous on 11/07/2020
Application: Sample Tested: Monocytes

Great product, good curve and results


Human IGF-I/IGF-1 DuoSet ELISA
By Anonymous on 11/07/2020
Application: Sample Tested: Peripheral blood monocytes

Easy to use and good results


Human IGF-I/IGF-1 DuoSet ELISA
By Anonymous on 10/28/2020
Application: Sample Tested: Cell culture supernatant

Kit was easy to use and IGF-1 was detectable in my PBMC cultures