Human IGFBP-3 Antibody

Catalog # Availability Size / Price Qty
AF675
AF675-SP
IGFBP‑3 in Human Colon.
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Product Details
Citations (11)
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Human IGFBP-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGFBP‑3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross reactivity with recombinant mouse IGFBP-3 is observed, and less than 1% cross-reactivity with recombinant human (rh) IGFBP‑1, rhIGFBP‑2, and rhIGFBP‑4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IGFBP‑3
Gly28-Lys291
Accession # CAA46087
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IGFBP‑3 (Catalog # 675-B3)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IGFBP‑3 inhibition of IGF‑II-dependent proliferation in the MCF‑7 human breast cancer cell line [Karey, K.P. et al. (1988) Cancer Research 48:4083]. The Neutralization Dose (ND50) is typically 0.75-3.0 µg/mL in the presence of 0.2 µg/mL Recombinant Human IGFBP‑3 and 14 ng/mL Recombinant Human IGF‑II.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry IGFBP‑3 antibody in Human Colon by Immunohistochemistry (IHC-P). View Larger

IGFBP‑3 in Human Colon. IGFBP‑3 was detected in immersion fixed paraffin-embedded sections of human colon using 10 µg/mL Human IGFBP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF675) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization IGFBP‑3 Inhibition of IGF‑II-dependent Cell Proliferation and Neutralization by Human IGFBP‑3 Antibody. View Larger

IGFBP‑3 Inhibition of IGF‑II-dependent Cell Proliferation and Neutralization by Human IGFBP‑3 Antibody. Recombinant Human IGFBP‑3 (Catalog # 675-B3) inhibits Recombinant Human IGF‑II (Catalog # 292-G2) induced proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF‑II (14 ng/mL) activity elicited by Recombinant Human IGFBP‑3 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Human IGFBP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF675). The ND50 is typically 0.75-3.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-3

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

Human IGFBP-3 cDNA encodes a 291 amino acid (aa) residue precursor protein with a putative 27 aa residue signal peptide that is processed to generate the 264 aa residue mature protein with three potential N-linked and two potential O-linked glycosylation sites. Human IGFBP-3 is expressed in multiple tissues. The highest expression level is found in the non-paranchymal cells of the liver. Expression levels are also higher during extrauterine life and peak during puberty. Human IGFBP-3 is the major IGF binding protein in plasma where it exists in a ternary complex with IGF-I or IGF-II and the acid-labile subunit (ALS).

References
  1. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
  2. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
  3. Spagnoli, A. and R.G. Rosenfeld (1997) Curr. Op. Endocrinology and Diabetes 4:1.
Long Name
Insulin-like Growth Factor Binding Protein 3
Entrez Gene IDs
3486 (Human); 16009 (Mouse)
Alternate Names
acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein; IBP-3; IBP3BP-53; IGF-binding protein 3; IGFBP3; IGFBP-3; insulin-like growth factor binding protein 3; insulin-like growth factor-binding protein 3

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Citations for Human IGFBP-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin system
    Authors: A Weigert, X Zheng, A Nenzel, K Turkowski, S Günther, E Strack, E Sirait-Fis, E Elwakeel, IM Kur, VS Nikam, C Valasaraja, H Winter, A Wissgott, R Voswinkel, F Grimminger, B Brüne, W Seeger, SS Pullamsett, R Savai
    Nature Communications, 2022-10-14;13(1):6078.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Netazepide Inhibits Expression of Pappalysin 2 in Type-1 Gastric Neuroendocrine Tumors
    Authors: KA Lloyd, BN Parsons, MD Burkitt, AR Moore, S Papoutsopo, M Boyce, CA Duckworth, K Exarchou, N Howes, L Rainbow, Y Fang, C Oxvig, S Dodd, A Varro, N Hall, DM Pritchard
    Cell Mol Gastroenterol Hepatol, 2020-01-28;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  3. ISL1 overexpression enhances the survival of transplanted human mesenchymal stem cells in a murine myocardial infarction model
    Authors: Q Xiang, Y Liao, H Chao, W Huang, J Liu, H Chen, D Hong, Z Zou, AP Xiang, W Li
    Stem Cell Res Ther, 2018-02-26;9(1):51.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  4. Amyloid beta-mediated epigenetic alteration of insulin-like growth factor binding protein 3 controls cell survival in Alzheimer's disease.
    Authors: Sung H, Choi E, Lyu D, Mook-Jung I, Ahn J
    PLoS ONE, 2014-06-25;9(6):e99047.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Antitumor activity and immunogenicity of recombinant vaccinia virus expressing HPV 16 E7 protein SigE7LAMP is enhanced by high-level coexpression of IGFBP-3.
    Authors: Musil, J, Kutinova, L, Zurkova, K, Hainz, P, Babiarova, K, Krystofova, J, Nemeckova, S
    Cancer Gene Ther, 2014-02-21;21(3):115-25.
    Species: Mouse, Primate - Chlorocebus aethiops (African Green Monkey)
    Sample Types: Whole Cells
    Applications: Neutralization
  6. The contributions of HIF-target genes to tumor growth in RCC.
    Authors: Zhang, Ting, Niu, Xiaohua, Liao, Lili, Cho, Eun-Ah, Yang, Haifeng
    PLoS ONE, 2013-11-18;8(11):e80544.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. The von Hippel-Lindau tumor suppressor protein regulates gene expression and tumor growth through histone demethylase JARID1C.
    Authors: Niu X, Zhang T, Liao L, Zhou L, Lindner DJ, Zhou M, Rini B, Yan Q, Yang H
    Oncogene, 2011-07-04;31(6):776-86.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. IGFBP-3 is a metastasis suppression gene in prostate cancer.
    Authors: Mehta HH, Gao Q, Galet C
    Cancer Res., 2011-06-22;71(15):5154-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Human IGF Binding Protein-3 Overexpression Impairs Glucose Regulation in Mice via an Inhibition of Insulin Secretion.
    Authors: Nguyen KH, Yao XH, Moulik S, Mishra S, Nyomba BL
    Endocrinology, 2011-03-29;152(6):2184-96.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Breast cancer cells induce cancer-associated fibroblasts to secrete hepatocyte growth factor to enhance breast tumorigenesis.
    Authors: Tyan SW, Kuo WH, Huang CK
    PLoS ONE, 2011-01-13;6(1):e15313.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  11. The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
    Authors: McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A
    Gastroenterology, 2006-03-06;130(6):1754-63.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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