Human IGFBP-4 Biotinylated Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IGFBP-4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant human (rh) IGFBP-1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IGFBP‑4
    Asp22-Glu258
    Accession # AAA62670
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human IGFBP‑4 (Catalog # 804-GB)
  • Immunohistochemistry
    5-15 µg/mL
    See below
    • Human IGFBP-4 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human IGFBP‑4 Antibody (Catalog # MAB8041)
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human IGFBP‑4 Antibody (Catalog # MAB8041R)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human IGFBP‑4 Biotinylated Antibody (Catalog # BAF804)
  • ELISA Standard
     
    Recombinant Human IGFBP-4 Protein, CF (Catalog # 804-GB)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
IGFBP‑4 in Human Stomach. IGFBP‑4 was detected in immersion fixed paraffin-embedded sections of human stomach array using Goat Anti-Human IGFBP‑4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF804) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGFBP-4

Human IGF binding protein 4 (IGFBP-4) was isolated from human plasma based on its ability to bind immobilized IGF-I. Human IGFBP-4 cDNA encodes a 258 amino acid (aa) residue precursor protein with a predicted 21 aa residue signal peptide that is processed to generate the 237 aa residue mature human IGFBP-4. The human IGFBP-4 contains a potential N-linked glycosylation site and shares approximately 90% amino acid sequence identity with both the mouse and rat IGFBP-4.

According to the nomenclature of IGFBPs defined at the 4th International Symposium of IGFs (1997, Tokyo), six high-affinity IGF binding proteins (IGFBP-1, -2, -3, -4, -5, -6), and four IGFBP-related proteins (IGFBPr-1, - 2, -3, -4) have been identified. All IGFBPs have a high cysteine content and share conserved cysteine residues that are clustered in the amino- and carboxy-terminal third of the molecule. IGFBPs have been shown to either inhibit or enhance the biological activities of IGF, or act in an IGF-independent manner. Post-translational modification of IGFBPs, including phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins for IGF and may indirectly regulate IGF actions.

  • References:
    1. Jones, J. I. and D.R. Clemons (1995) Endocrine Rev. 16:3.
    2. Kelly, K.M. et al. (1996) Intl. J. Biochem. Cell Biol. 28:619.
    3. Kim, H-S. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12981.
  • Long Name:
    Insulin-like Growth Factor Binding Protein 4
  • Entrez Gene IDs:
    3487 (Human); 16010 (Mouse)
  • Alternate Names:
    BP-4; HT29-IGFBP; IBP4insulin-like growth factor-binding protein 4; IGF-binding protein 4; IGFBP4; IGFBP-4; IGFBP-4IBP-4; insulin-like growth factor binding protein 4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Steroids and protein markers in the follicular fluid as indicators of oocyte quality in patients with and without endometriosis.
    Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
    J. Assist. Reprod. Genet., 2005;22(6):257-64.
    Species: Human
    Sample Type: Follicular Fluid
    Application: ELISA Development
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 4
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Biotinylated Control

Ctrl BAF108 19
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Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Phycoerythrin

Flow F0040 7
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP

ELISA DY998 44
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EvenCoat Streptavidin Coated Plates

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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