Human IGFBP-rp1/IGFBP-7 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY1334-05
Ancillary Products Available
Human IGFBP-rp1 / IGFBP-7 ELISA Standard Curve
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Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (2)

Human IGFBP-rp1/IGFBP-7 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
39.1 - 2,500 pg/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IGFBP-rp1 / IGFBP-7. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human IGFBP-rp1 / IGFBP-7 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGFBP-rp1/IGFBP-7

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. ALS (Acid Labile Subunit) is a liver-derived protein that exists in a ternary complex with Insulin-like Growth Factor (IGF)-binding Protein-3 (IGFBP-3) or IGFBP-5, and either IGF-I or IGF-II. ALS increases the half-life of IGF/IGFBP complexes in circulation.

Long Name:
Insulin-like Growth Factor Binding Protein Related Protein 1
Entrez Gene IDs:
3490 (Human); 29817 (Mouse)
Alternate Names:
angiomodulin; FSTL2; IBP-7; IGFBP7; IGFBP-7; IGFBP-7PGI2-stimulating factor; IGFBP-7v; IGFBPrp1; IGFBP-rp1; insulin-like growth factor binding protein 7; insulin-like growth factor-binding protein 7; MAC25 protein; Mac25; MAC25IGF-binding protein 7; Prostacyclin-stimulating factor; PSF; PSFAGM; TAF; Tumor-derived adhesion factor

Citations for Human IGFBP-rp1/IGFBP-7 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Derivation and validation of urinary TIMP-1 for the prediction of acute kidney injury and mortality in critically ill children
    Authors: H Huang, Q Lin, X Dai, J Chen, Z Bai, X Li, F Fang, Y Li
    Journal of Translational Medicine, 2022;20(1):102.
    Species: Human
    Sample Types: Urine
  2. Remote ischemic preconditioning causes transient cell-cycle arrest and renal protection by a NF-kB-dependent Sema5B pathway
    Authors: J Rossaint, M Meersch, K Thomas, S Mersmann, M Lehmann, J Skupski, T Tekath, P Rosenberge, JA Kellum, H Pavenstädt, A Zarbock
    JCI Insight, 2022;0(0):.
    Species: Mouse
    Sample Types: Serum
  3. Combining renal cell arrest and damage biomarkers to predict progressive AKI in patient with sepsis
    Authors: X Tao, C Chen, W Luo, J Zhou, J Tian, X Yang, FF Hou
    Bmc Nephrology, 2021;22(1):415.
    Species: Human
    Sample Types: Urine
  4. Urine Biomarkers for the Assessment of Acute Kidney Injury in Neonates with Hypoxic Ischemic Encephalopathy Receiving Therapeutic Hypothermia
    Authors: J Rumpel, BJ Spray, VY Chock, MJ Kirkley, CL Slagle, A Frymoyer, SH Cho, KM Gist, R Blaszak, B Poindexter, SE Courtney
    The Journal of pediatrics, 2021;0(0):.
    Species: Human
    Sample Types: Urine
  5. Serum and urine FGF23 and IGFBP-7 for the prediction of acute kidney injury in critically ill children
    Authors: Z Bai, F Fang, Z Xu, C Lu, X Wang, J Chen, J Pan, J Wang, Y Li
    BMC Pediatr, 2018;18(1):192.
    Species: Human
    Sample Types: Serum

FAQs

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Reviews for Human IGFBP-rp1/IGFBP-7 DuoSet ELISA

Average Rating: 5 (Based on 2 Reviews)

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Human IGFBP-rp1/IGFBP-7 DuoSet ELISA
By Anonymous on 10/01/2020
Sample Tested: Urine

Fantastic performance. Remember this uses reagent kit 3 not 2 (most of the duosets I use require to 2) which messed me up at first. Used for human urine @ 1:500 dilution.


Human IGFBP-rp1/IGFBP-7 DuoSet ELISA
By Rambabu Surabattula on 09/13/2019
Sample Tested: Human serum

I used this kit for quantifying IGFBP7 in human serum. It works very well and easy to use protocol. I have used almost 6X 5kit so far.