Human IGFBP-rp1/IGFBP-7 DuoSet ELISA

R&D Systems | Catalog # DY1334-05

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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

39.1-2500 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human IGFBP-rp1/IGFBP-7 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all IGFBP-rp1/IGFBP-7 ELISA Kits »

Product Summary for Human IGFBP-rp1/IGFBP-7 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IGFBP-rp1 / IGFBP-7. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human IGFBP-rp1/IGFBP-7 DuoSet ELISA

Human IGFBP-rp1 / IGFBP-7 ELISA Standard Curve

Human IGFBP-rp1 / IGFBP-7 ELISA Standard Curve

Kit Contents for Human IGFBP-rp1/IGFBP-7 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 3 (5 plates): (Catalog # DY009C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 3.

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)

Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)

Microplates: (Catalog # DY990), or equivalent

Plate Sealers: (Catalog # DY992), or equivalent

*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGFBP-rp1/IGFBP-7

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. ALS (Acid Labile Subunit) is a liver-derived protein that exists in a ternary complex with Insulin-like Growth Factor (IGF)-binding Protein-3 (IGFBP-3) or IGFBP-5, and either IGF-I or IGF-II. ALS increases the half-life of IGF/IGFBP complexes in circulation.

Long Name

Insulin-like Growth Factor Binding Protein Related Protein 1

Alternate Names

IGFBP-7, IGFBP7, IGFBPrp1, Mac25, PSF

Entrez Gene IDs

3490 (Human); 29817 (Mouse)

Gene Symbol

IGFBP7

Additional IGFBP-rp1/IGFBP-7 Products

Product Documents for Human IGFBP-rp1/IGFBP-7 DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human IGFBP-rp1/IGFBP-7 DuoSet ELISA

For research use only

Citations for Human IGFBP-rp1/IGFBP-7 DuoSet ELISA

Customer Reviews for Human IGFBP-rp1/IGFBP-7 DuoSet ELISA (3)

4.7 out of 5
3 Customer Ratings
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4 Stars
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  • Human IGFBP-rp1/IGFBP-7 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 07/01/2024
    Works very well for serum and plasma at higher dilutions.
    Human IGFBP-rp1/IGFBP-7 DuoSet ELISA DY1334-05
  • Human IGFBP-rp1/IGFBP-7 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Urine
    Verified Customer | Posted 10/01/2020
    Fantastic performance. Remember this uses reagent kit 3 not 2 (most of the duosets I use require to 2) which messed me up at first. Used for human urine @ 1:500 dilution.
    Human IGFBP-rp1/IGFBP-7 DuoSet ELISA DY1334-05
  • Human IGFBP-rp1/IGFBP-7 DuoSet ELISA
    Name: Rambabu Surabattula
    Sample Tested: Human serum
    Verified Customer | Posted 09/13/2019
    I used this kit for quantifying IGFBP7 in human serum. It works very well and easy to use protocol. I have used almost 6X 5kit so far.
    Human IGFBP-rp1/IGFBP-7 DuoSet ELISA DY1334-05

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