Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit

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PDLA50
SLA50
DLA50
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Human IL-1 alpha/IL-1F1 ELISA Calibrator Diluent RD5-5 Standard Curve
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Citations (27)
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Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)
Sensitivity
1 pg/mL
Assay Range
3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human IL-1 alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human IL-1 alpha Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-1 alpha in cell culture supernates, serum, and plasma. It contains recombinant human IL-1 alpha and antibodies raised against recombinant human IL-1 alpha and has been shown to accurately quantitate the recombinant factor. Results obtained using natural IL-1 alpha showed linear curves that were parallel to the standard curves obtained using the E. coli-expressed Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-1 alpha.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 28.6 125 185 27.9 123 187
Standard Deviation 0.4 1.4 4 1.2 4.7 6.3
CV% 1.4 1.1 2.2 4.3 3.8 3.4

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 26.9 122 191 20.5 60 121
Standard Deviation 0.4 4.6 6.6 1.7 3.1 5.2
CV% 1.5 3.8 3.5 8.3 5.2 4.3

Recovery

The recovery of IL-1 alpha spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 91-104
Citrate Plasma (n=6) 97 71-119
EDTA Plasma (n=6) 104 84-121
Heparin Plasma (n=6) 104 72-126
Serum (n=6) 107 74-127

Linearity

To assess the linearity of the assay, the following biological samples spiked with high concentrations of IL-1 alpha were diluted with the appropriate Calibrator Diluent and then assayed.
Human IL-1 alpha/IL-1F1 ELISA Linearity

Data Examples

Human IL-1 alpha/IL-1F1 ELISA Calibrator Diluent RD5-5 Standard Curve

Human IL-1 alpha/IL-1F1 ELISA Calibrator Diluent RD6C Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 alpha/IL-1F1

IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signaling.

Long Name:
Interleukin 1 alpha
Entrez Gene IDs:
3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)
Alternate Names:
Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 200 µL Standard, Control, or Sample
  6.   Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well.
  10.   For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour.
    For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  11.   Aspirate and wash 3 times.

  12. 200 µL Substrate Solution
  13.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

  14. 50 µL Stop Solution
  15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Multiple Cold-Water Immersions Attenuate Muscle Damage but not Alter Systemic Inflammation and Muscle Function Recovery: A Parallel Randomized Controlled Trial
    Authors: AF Siqueira, A Vieira, M Bottaro, JB Ferreira-J, OT Nóbrega, VC de Souza, RC Marqueti, N Babault, JLQ Durigan
    Sci Rep, 2018;8(1):10961.
    Species: Human
    Sample Types: Serum
  2. Antibody Array Revealed PRL-3 Affects Protein Phosphorylation and Cytokine Secretion
    Authors: Y Yang, S Lian, L Meng, L Qu, C Shou
    PLoS ONE, 2017;12(1):e0169665.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Nuclear DNA damage-triggered NLRP3 inflammasome activation promotes UVB-induced inflammatory responses in human keratinocytes
    Authors: Tatsuya Hasegawa
    Biochem Biophys Res Commun, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. MyD88-Dependent Signaling Decreases the Antitumor Efficacy of Epidermal Growth Factor Receptor Inhibition in Head and Neck Cancer Cells.
    Authors: Koch A, Love-Homan L, Espinosa-Cotton M, Stanam A, Simons A
    Cancer Res, 2015;75(8):1657-67.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Early efficacy trial of anakinra in corticosteroid-resistant autoimmune inner ear disease.
    Authors: Vambutas A, Lesser M, Mullooly V, Pathak S, Zahtz G, Rosen L, Goldofsky E
    J Clin Invest, 2014;124(9):4115-22.
    Species: Human
    Sample Types: Plasma
  6. Role of IL-1alpha and the Nlrp3/caspase-1/IL-1beta axis in cigarette smoke-induced pulmonary inflammation and COPD.
    Authors: Pauwels NS, Bracke KR, Dupont LL, Van Pottelberge GR, Provoost S, Vanden Berghe T, Vandenabeele P, Lambrecht BN, Joos GF, Brusselle GG
    Eur. Respir. J., 2011;38(5):1019-28.
    Species: Human
    Sample Types: Sputum
  7. RAS mutations affect tumor necrosis factor-induced apoptosis in colon carcinoma cells via ERK-modulatory negative and positive feedback circuits along with non-ERK pathway effects.
    Authors: Kreeger PK, Mandhana R, Alford SK, Haigis KM, Lauffenburger DA
    Cancer Res., 2009;69(20):8191-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. In vivo regulation of interleukin 1beta in patients with cryopyrin-associated periodic syndromes.
    Authors: Lachmann HJ, Lowe P, Felix SD, Rordorf C, Leslie K, Madhoo S, Wittkowski H, Bek S, Hartmann N, Bosset S, Hawkins PN, Jung T
    J. Exp. Med., 2009;206(5):1029-36.
    Species: Human
    Sample Types: Plasma
  9. HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
    Authors: Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A
    J. Exp. Med., 2009;206(1):169-81.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Selective inhibition of proprotein convertases represses the metastatic potential of human colorectal tumor cells.
    Authors: Scamuffa N, Siegfried G, Bontemps Y, Ma L, Basak A, Cherel G, Calvo F, Seidah NG, Khatib AM
    J. Clin. Invest., 2008;118(1):352-63.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Protection of human keratinocytes from UVB-induced inflammation using root extract of Lithospermum erythrorhizon.
    Authors: Ishida T, Sakaguchi I,
    Biol. Pharm. Bull., 2007;30(5):928-34.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells.
    Authors: Arnold R, Neumann M, Konig W
    Immunology, 2007;121(1):71-81.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Administration of cyclooxygenase-2 inhibitor reduces joint inflammation but exacerbates osteopenia in IL-1 alpha transgenic mice due to GM-CSF overproduction.
    Authors: Niki Y, Takaishi H, Takito J, Miyamoto T, Kosaki N, Matsumoto H, Toyama Y, Tada N
    J. Immunol., 2007;179(1):639-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. The influence of anger expression on wound healing.
    Authors: Gouin JP, Kiecolt-Glaser JK, Malarkey WB, Glaser R
    Brain Behav. Immun., 2007;22(5):699-708.
    Species: Human
    Sample Types: Tissue Secretion
  15. The response of human epithelial cells to TNF involves an inducible autocrine cascade.
    Authors: Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK
    Cell, 2006;124(6):1225-39.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Inhibitory effects of micronized fenofibrate on carotid atherosclerosis in patients with essential hypertension.
    Authors: Zhu S, Su G, Meng QH
    Clin. Chem., 2006;52(11):2036-42.
    Species: Human
    Sample Types: Serum
  17. Circulating and synovial levels of IGF-I, cytokines, physical function and anthropometry differ in women awaiting total knee arthroplasty when compared to men.
    Authors: Pagura SM, Thomas SG, Woodhouse LJ, Ezzat S, Marks P
    J. Orthop. Res., 2005;23(2):397-405.
    Species: Human
    Sample Types: Synovial Fluid
  18. Effects of scaling and root planing on the amounts of interleukin-1 and interleukin-1 receptor antagonist and the mRNA expression of interleukin-1beta in gingival crevicular fluid and gingival tissues.
    Authors: Yoshinari N, Kawase H, Mitani A, Ito M, Sugiishi S, Matsuoka M, Shirozu N, Ishihara Y, Bito B, Hiraga M, Arakawa K, Noguchi T
    J. Periodont. Res., 2004;39(3):158-67.
    Species: Human
    Sample Types: Gingival Crevicular Fluid (GCF)
  19. Guiding the vaginal microbicide trials with biomarkers of inflammation.
    Authors: Fichorova RN
    J. Acquir. Immune Defic. Syndr., 2004;37(0):S184-93.
    Species: Human
    Sample Types: Vaginal Lavage
  20. The soluble form of IL-1 receptor accessory protein enhances the ability of soluble type II IL-1 receptor to inhibit IL-1 action.
    Authors: Smith DE, Hanna R, Della Friend, Moore H, Chen H, Farese AM, MacVittie TJ, Virca GD, Sims JE
    Immunity, 2003;18(1):87-96.
    Species: Human
    Sample Types: Serum
  21. Retroviral interleukin 1alpha gene transfer in bone marrow stromal cells in a primate model: induction of myelopoiesis stimulation.
    Authors: de Revel T, Becard N, Sorg T, Rousseau S, Spano JP, Thiebot H, Methali M, Gras G, Le Grand R, Dormont D
    Br. J. Haematol., 2002;118(3):875-84.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Cell Culture Supernates
  22. High versus low basal cortisol secretion in asymptomatic, medication-free HIV-infected men: differential effects of severe life stress on parameters of immune status.
    Authors: Petitto JM, Leserman J, Perkins DO, Stern RA, Silva SG, Gettes D, Zheng B, Folds JD, Golden RN, Evans DL
    Behav Med, 2000;25(4):143-51.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Characterization of bone resorbing activity in gingival crevicular fluid from patients with periodontitis.
    Authors: Rasmussen L, Hanstrom L, Lerner UH
    J. Clin. Periodontol., 2000;27(1):41-52.
    Species: Human
    Sample Types: Gingival Crevicular Fluid (GCF)
  24. Cytokine profiles in parotid saliva from HIV-1-infected individuals: changes associated with opportunistic infections in the oral cavity.
    Authors: Black, K P, Merrill, K W, Jackson, S, Katz, J
    Oral Microbiol Immunol, 2000;15(2):74-81.
    Species: Human
    Sample Types: Saliva
  25. c-Jun and JunB antagonistically control cytokine-regulated mesenchymal-epidermal interaction in skin.
    Authors: Szabowski A, Maas-Szabowski N, Andrecht S, Kolbus A, Schorpp-Kistner M, Fusenig NE, Angel P
    Cell, 2000;103(5):745-55.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
    Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
    J. Exp. Med., 2000;192(2):219-26.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Gingival crevicular fluid from patients with periodontitis contains bone resorbing activity.
    Authors: Lerner UH, Modeer T, Krekmanova L, Claesson R, Rasmussen L
    Eur. J. Oral Sci., 1998;106(3):778-87.
    Species: Human
    Sample Types: Gingival Crevicular Fluid (GCF)

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Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit
By Anonymous on 08/06/2018
Sample Tested: Serum

Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit
By Anonymous on 07/11/2018
Sample Tested: A-673 human Ewing's sarcoma cell line

Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit
By Anonymous on 06/27/2018
Sample Tested: Pancreatic cancer cells

Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit
By Anonymous on 04/11/2018
Sample Tested: Adipocytes

Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit
By Anonymous on 03/30/2018
Sample Tested: PANC-1 human pancreatic carcinoma cell line

Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit
By Anonymous on 12/07/2017
Sample Tested: CHO Chinese hamster ovary cell line