hh Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit DLA50: R&D Systems

Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit

(26 citations)
(1 Review)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3.5 hours or 4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)
  • Sensitivity
    1 pg/mL
  • Assay Range
    3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human IL-1 alpha
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC01-1, Quantikine Immunoassay Control Group 1 - Please Inquire
Product Summary
The Quantikine Human IL-1 alpha Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-1 alpha in cell culture supernates, serum, and plasma. It contains recombinant human IL-1 alpha and antibodies raised against recombinant human IL-1 alpha and has been shown to accurately quantitate the recombinant factor. Results obtained using natural IL-1 alpha showed linear curves that were parallel to the standard curves obtained using the E. coli-expressed Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-1 alpha.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean26.912219120.560121
Standard Deviation0.44.66.61.73.15.2
CV%1.53.83.58.35.24.3

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean28.612518527.9123187
Standard Deviation0.41.441.24.76.3
CV%1.41.12.24.33.83.4

Recovery

The recovery of IL-1 alpha spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 91-104
Citrate Plasma (n=6) 97 71-119
EDTA Plasma (n=6) 104 84-121
Heparin Plasma (n=6) 104 72-126
Serum (n=6) 107 74-127
Linearity
To assess the linearity of the assay, the following biological samples spiked with high concentrations of IL-1 alpha were diluted with the appropriate Calibrator Diluent and then assayed.
Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-1 alpha/IL-1F1
IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signaling.
    • Long Name
      Interleukin 1 alpha
    • Entrez Gene IDs
      3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine);
    • Alternate Names
      Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 200 µL Standard, Control, or Sample
    6.   Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well.
    10.   For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour.
      For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    11.   Aspirate and wash 3 times.

    12. 200 µL Substrate Solution
    13.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

    14. 50 µL Stop Solution
    15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    26 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Antibody Array Revealed PRL-3 Affects Protein Phosphorylation and Cytokine Secretion
      Authors: Y Yang, S Lian, L Meng, L Qu, C Shou
      PLoS ONE, 2017;12(1):e0169665.
      Species: Human
      Sample Type: Cell Culture Supernates
    2. Nuclear DNA damage-triggered NLRP3 inflammasome activation promotes UVB-induced inflammatory responses in human keratinocytes
      Authors: Tatsuya Hasegawa
      Biochem Biophys Res Commun, 2016;0(0):.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. MyD88-Dependent Signaling Decreases the Antitumor Efficacy of Epidermal Growth Factor Receptor Inhibition in Head and Neck Cancer Cells.
      Authors: Koch A, Love-Homan L, Espinosa-Cotton M, Stanam A, Simons A
      Cancer Res, 2015;75(8):1657-67.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. Early efficacy trial of anakinra in corticosteroid-resistant autoimmune inner ear disease.
      Authors: Vambutas A, Lesser M, Mullooly V, Pathak S, Zahtz G, Rosen L, Goldofsky E
      J Clin Invest, 2014;124(9):4115-22.
      Species: Human
      Sample Type: Plasma
    5. Role of IL-1alpha and the Nlrp3/caspase-1/IL-1beta axis in cigarette smoke-induced pulmonary inflammation and COPD.
      Authors: Pauwels NS, Bracke KR, Dupont LL, Van Pottelberge GR, Provoost S, Vanden Berghe T, Vandenabeele P, Lambrecht BN, Joos GF, Brusselle GG
      Eur. Respir. J., 2011;38(5):1019-28.
      Species: Human
      Sample Type: Sputum
    6. In vivo regulation of interleukin 1beta in patients with cryopyrin-associated periodic syndromes.
      Authors: Lachmann HJ, Lowe P, Felix SD, Rordorf C, Leslie K, Madhoo S, Wittkowski H, Bek S, Hartmann N, Bosset S, Hawkins PN, Jung T
      J. Exp. Med., 2009;206(5):1029-36.
      Species: Human
      Sample Type: Plasma
    7. HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
      Authors: Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A
      J. Exp. Med., 2009;206(1):169-81.
      Species: Human
      Sample Type: Cell Culture Supernates
    8. RAS mutations affect tumor necrosis factor-induced apoptosis in colon carcinoma cells via ERK-modulatory negative and positive feedback circuits along with non-ERK pathway effects.
      Authors: Kreeger PK, Mandhana R, Alford SK, Haigis KM, Lauffenburger DA
      Cancer Res., 2009;69(20):8191-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. Selective inhibition of proprotein convertases represses the metastatic potential of human colorectal tumor cells.
      Authors: Scamuffa N, Siegfried G, Bontemps Y, Ma L, Basak A, Cherel G, Calvo F, Seidah NG, Khatib AM
      J. Clin. Invest., 2008;118(1):352-63.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells.
      Authors: Arnold R, Neumann M, Konig W
      Immunology, 2007;121(1):71-81.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. The influence of anger expression on wound healing.
      Authors: Gouin JP, Kiecolt-Glaser JK, Malarkey WB, Glaser R
      Brain Behav. Immun., 2007;22(5):699-708.
      Species: Human
      Sample Type: Tissue Secretion
    12. Administration of cyclooxygenase-2 inhibitor reduces joint inflammation but exacerbates osteopenia in IL-1 alpha transgenic mice due to GM-CSF overproduction.
      Authors: Niki Y, Takaishi H, Takito J, Miyamoto T, Kosaki N, Matsumoto H, Toyama Y, Tada N
      J. Immunol., 2007;179(1):639-46.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. Protection of human keratinocytes from UVB-induced inflammation using root extract of Lithospermum erythrorhizon.
      Authors: Ishida T, Sakaguchi I,
      Biol. Pharm. Bull., 2007;30(5):928-34.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Inhibitory effects of micronized fenofibrate on carotid atherosclerosis in patients with essential hypertension.
      Authors: Zhu S, Su G, Meng QH
      Clin. Chem., 2006;52(11):2036-42.
      Species: Human
      Sample Type: Serum
    15. The response of human epithelial cells to TNF involves an inducible autocrine cascade.
      Authors: Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK
      Cell, 2006;124(6):1225-39.
      Species: Human
      Sample Type: Cell Culture Supernates
    16. Circulating and synovial levels of IGF-I, cytokines, physical function and anthropometry differ in women awaiting total knee arthroplasty when compared to men.
      Authors: Pagura SM, Thomas SG, Woodhouse LJ, Ezzat S, Marks P
      J. Orthop. Res., 2005;23(2):397-405.
      Species: Human
      Sample Type: Synovial Fluid
    17. Guiding the vaginal microbicide trials with biomarkers of inflammation.
      Authors: Fichorova RN
      J. Acquir. Immune Defic. Syndr., 2004;37(0):S184-93.
      Species: Human
      Sample Type: Vaginal Lavage
    18. Effects of scaling and root planing on the amounts of interleukin-1 and interleukin-1 receptor antagonist and the mRNA expression of interleukin-1beta in gingival crevicular fluid and gingival tissues.
      Authors: Yoshinari N, Kawase H, Mitani A, Ito M, Sugiishi S, Matsuoka M, Shirozu N, Ishihara Y, Bito B, Hiraga M, Arakawa K, Noguchi T
      J. Periodont. Res., 2004;39(3):158-67.
      Species: Human
      Sample Type: Gingival Crevicular Fluid (GCF)
    19. The soluble form of IL-1 receptor accessory protein enhances the ability of soluble type II IL-1 receptor to inhibit IL-1 action.
      Authors: Smith DE, Hanna R, Della Friend, Moore H, Chen H, Farese AM, MacVittie TJ, Virca GD, Sims JE
      Immunity, 2003;18(1):87-96.
      Species: Human
      Sample Type: Serum
    20. Retroviral interleukin 1alpha gene transfer in bone marrow stromal cells in a primate model: induction of myelopoiesis stimulation.
      Authors: de Revel T, Becard N, Sorg T, Rousseau S, Spano JP, Thiebot H, Methali M, Gras G, Le Grand R, Dormont D
      Br. J. Haematol., 2002;118(3):875-84.
      Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
      Sample Type: Cell Culture Supernates
    21. Cytokine profiles in parotid saliva from HIV-1-infected individuals: changes associated with opportunistic infections in the oral cavity.
      Authors: Black, K P, Merrill, K W, Jackson, S, Katz, J
      Oral Microbiol Immunol, 2000;15(2):74-81.
      Species: Human
      Sample Type: Saliva
    22. High versus low basal cortisol secretion in asymptomatic, medication-free HIV-infected men: differential effects of severe life stress on parameters of immune status.
      Authors: Petitto JM, Leserman J, Perkins DO, Stern RA, Silva SG, Gettes D, Zheng B, Folds JD, Golden RN, Evans DL
      Behav Med, 2000;25(4):143-51.
      Species: Human
      Sample Type: Cell Culture Supernates
    23. c-Jun and JunB antagonistically control cytokine-regulated mesenchymal-epidermal interaction in skin.
      Authors: Szabowski A, Maas-Szabowski N, Andrecht S, Kolbus A, Schorpp-Kistner M, Fusenig NE, Angel P
      Cell, 2000;103(5):745-55.
      Species: Human
      Sample Type: Cell Culture Supernates
    24. Characterization of bone resorbing activity in gingival crevicular fluid from patients with periodontitis.
      Authors: Rasmussen L, Hanstrom L, Lerner UH
      J. Clin. Periodontol., 2000;27(1):41-52.
      Species: Human
      Sample Type: Gingival Crevicular Fluid (GCF)
    25. Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
      Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
      J. Exp. Med., 2000;192(2):219-26.
      Species: Human
      Sample Type: Cell Culture Supernates
    26. Gingival crevicular fluid from patients with periodontitis contains bone resorbing activity.
      Authors: Lerner UH, Modeer T, Krekmanova L, Claesson R, Rasmussen L
      Eur. J. Oral Sci., 1998;106(3):778-87.
      Species: Human
      Sample Type: Gingival Crevicular Fluid (GCF)
    Expand to show all 26 Citations
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    Sample TestedCHO Chinese hamster ovary cell line

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