Human IL-1 beta/IL-1F2 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IL-1 beta/IL-1F2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Data Example
Product Datasheets
Preparation and Storage
Background: IL-1 beta/IL-1F2
The Interleukin 1 (IL-1) family of proteins consists of the classic members IL-1 alpha, IL-1 beta, and IL-1ra, plus IL-18, IL-33 and IL-1F5-F10. IL-1 alpha and IL-1 beta bind to the same cell surface receptors and share biological functions. IL-1 is not produced by unstimulated cells of healthy individuals with the exception of skin keratinocytes, some epithelial cells, and certain cells of the central nervous system. However, in response to inflammatory agents, infections, or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cell types is observed. IL-1 beta plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. Inappropriate or prolonged production of IL-1 has been implicated in a variety of pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.
Assay Procedure
GENERAL ELISA PROTOCOL
Plate Preparation
- Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
- Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
- Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
- Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.
Assay Procedure
- Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Repeat the aspiration/wash as in step 2.
- Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
- Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
Citations for Human IL-1 beta/IL-1F2 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
163
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
The non-saponin fraction of Korean Red Ginseng (KGC05P0) decreases glucose uptake and transport in�vitro and modulates glucose production via down-regulation of the PI3K/AKT pathway in�vivo
Authors: SJ Park, D Lee, D Kim, M Lee, G In, ST Han, SW Kim, MH Lee, OK Kim, J Lee
J Ginseng Res, 2020;44(2):362-372.
Species: Human
Sample Types: Serum
-
Reprogramming of profibrotic macrophages for treatment of bleomycin-induced pulmonary fibrosis
Authors: F Zhang, EA Ayaub, B Wang, E Puchulu-Ca, YH Li, SU Hettiarach, SD Lindeman, Q Luo, S Rout, M Srinivasar, A Cox, K Tsoyi, C Nickerson-, IO Rosas, PS Low
EMBO Mol Med, 2020;0(0):e12034.
Species: Human
Sample Types: Cell Culture Supernates
-
Recombinant Alkaline Phosphatase Prevents Acute on Chronic Liver Failure
Authors: C Engelmann, D Adebayo, M Oria, F De Chiara, S Novelli, A Habtesion, N Davies, F Andreola, R Jalan
Sci Rep, 2020;10(1):389.
Species: Human
Sample Types: Cell Culture Supernates
-
PB1-F2 protein of highly pathogenic influenza A (H7N9) virus selectively suppresses RNA-induced NLRP3 inflammasome activation through inhibition of MAVS-NLRP3 interaction
Authors: PH Cheung, ZW Ye, TT Lee, H Chen, CP Chan, DY Jin
J. Leukoc. Biol., 2020;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Intracellular Insulin-like growth factor binding protein 2 (IGFBP2) contributes to the senescence of keratinocytes in psoriasis by stabilizing cytoplasmic p21
Authors: L Mercurio, D Lulli, F Mascia, E Dellambra, C Scarponi, M Morelli, C Valente, ML Carbone, S Pallotta, G Girolomoni, C Albanesi, S Pastore, S Madonna
Aging (Albany NY), 2020;12(8):6823-6851.
Species: Human
Sample Types: Cell Culture Supernates
-
N-GSDMD trafficking to neutrophil organelles facilitates IL-1&beta release independently of plasma membrane pores and pyroptosis
Authors: M Karmakar, M Minns, EN Greenberg, J Diaz-Apont, K Pestonjama, JL Johnson, JK Rathkey, DW Abbott, K Wang, F Shao, SD Catz, GR Dubyak, E Pearlman
Nat Commun, 2020;11(1):2212.
Species: Human
Sample Types: Cell Culture Supernates
-
Activation of Human &gamma&delta T Cells: Modulation by Toll-Like Receptor 8 Ligands and Role of Monocytes
Authors: R Serrano, D Wesch, D Kabelitz
Cells, 2020;9(3):.
Species: Human
Sample Types: Cell Culture Supernates
-
Cigarette smoke and extracellular Hsp70 induce secretion of ATP and differential activation of NLRP3 inflammasome in monocytic and bronchial epithelial cells
Authors: L Rumora, A Somborac-B, I Hlap?i?, A Hulina-Tom, MG Rajkovi?
Cytokine, 2020;135(0):155220.
Species: Human
Sample Types: Cell Culture Supernates
-
Pro-inflammatory properties of H-ferritin on human macrophages, ex vivo and in vitro observations
Authors: P Ruscitti, P Di Benedet, O Berardicur, N Panzera, N Grazia, AR Lizzi, P Cipriani, Y Shoenfeld, R Giacomelli
Sci Rep, 2020;10(1):12232.
Species: Human
Sample Types: Cell Culture Supernates
-
MG53 suppresses interferon-&beta and inflammation via regulation of ryanodine receptor-mediated intracellular calcium signaling
Authors: M Sermershei, AD Kenney, PH Lin, TM McMichael, C Cai, K Gumpper, TMA Adesanya, H Li, X Zhou, KH Park, JS Yount, J Ma
Nat Commun, 2020;11(1):3624.
Species: Human
Sample Types: Cell Culture Supernates
-
Differentially Expressed Genes of Natural Killer Cells Can Distinguish Rheumatoid Arthritis Patients from Healthy Controls
Authors: NM Elemam, MY Hachim, S Hannawi, AA Maghazachi
Genes (Basel), 2020;11(5):.
Species: Human
Sample Types: Plasma
-
Loss of function of the mitochondrial peptidase PITRM1 induces proteotoxic stress and Alzheimer's disease-like pathology in human cerebral organoids
Authors: MJ Pérez, D Ivanyuk, V Panagiotak, G Di Napoli, S Kalb, D Brunetti, R Al-Shaana, SA Kaeser, SA Fraschka, M Jucker, M Zeviani, C Viscomi, M Deleidi
Mol. Psychiatry, 2020;0(0):.
Species: Human
Sample Types: Cell Culture Lysates
-
Cerebrospinal fluid of chronic osteoarthritic patients induced interleukin-6 release in human glial cell-line T98G
Authors: W Liu, C Li, FCK Tan, HJ Neo, YH Chan, CM Low, TL Lee
BMC Anesthesiol, 2020;20(1):69.
Species: Human
Sample Types: Cell Culture Supernates
-
Role of vimentin in modulating immune cell apoptosis and inflammatory responses in sepsis
Authors: L Su, P Pan, P Yan, Y Long, X Zhou, X Wang, R Zhou, B Wen, L Xie, D Liu
Sci Rep, 2019;9(1):5747.
Species: Human
Sample Types: Cell Culture Supernates
-
Disrupting prolonged sitting reduces IL-8 and lower leg swell in active young adults
Authors: S Dogra, M Wolf, MP Jeffrey, RCA Foley, H Logan-Spre, H Jones-Tagg, JM Green-John
BMC Sports Sci Med Rehabil, 2019;11(0):23.
Species: Human
Sample Types: Saliva
-
Regulation of radiosensitivity by 4-methylumbelliferone via the suppression of interleukin-1 in fibrosarcoma cells
Authors: R Saga, K Hasegawa, K Murata, M Chiba, T Nakamura, K Okumura, E Tsuruga, Y Hosokawa
Oncol Lett, 2019;17(3):3555-3561.
Species: Human
Sample Types: Cell Culture Supernates
-
Replication of Genome-Wide Association Analysis Identifies New Susceptibility Loci at Long Noncoding RNA Regions for Vogt-Koyanagi-Harada Disease
Authors: J Qi, L Du, J Deng, Y Qin, G Su, S Hou, M Lv, Q Zhang, A Kijlstra, P Yang
Invest. Ophthalmol. Vis. Sci., 2019;60(14):4820-4829.
Species: Human
Sample Types: Cell Culture Supernates
-
Cervical cancer-instructed stromal fibroblasts enhance IL-23 expression in dendritic cells to support expansion of Th17 cells
Authors: B Walch-Rück, R Ströder, L Theobald, J Pahne-Zepp, S Hegde, YJ Kim, RM Bohle, I Juhasz-Bös, EF Solomayer, S Smola
Cancer Res., 2019;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Physiologic intestinal 18F-FDG uptake is associated with alteration of gut microbiota and proinflammatory cytokine levels in breast cancer
Authors: HJ Yoon, HN Kim, JI Bang, W Lim, BI Moon, NS Paik, BS Kim, HL Kim
Sci Rep, 2019;9(1):18273.
Species: Human
Sample Types: Serum
-
Glutathione Transferase Omega-1 Regulates NLRP3 Inflammasome Activation through NEK7 Deglutathionylation
Authors: MM Hughes, A Hooftman, S Angiari, P Tummala, Z Zaslona, MC Runtsch, AF McGettrick, CE Sutton, C Diskin, M Rooke, S Takahashi, S Sundararaj, MG Casarotto, JE Dahlstrom, EM Palsson-Mc, SC Corr, KHG Mills, RJS Preston, N Neamati, Y Xie, JB Baell, PG Board, LAJ O'Neill
Cell Rep, 2019;29(1):151-161.e5.
Species: Human
Sample Types: Cell Culture Supernates
-
IL-37 is increased in brains of children with autism spectrum disorder and inhibits human microglia stimulated by neurotensin
Authors: I Tsilioni, AB Patel, H Pantazopou, S Berretta, P Conti, SE Leeman, TC Theoharide
Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Dampened NLRP3-mediated inflammation in bats and implications for a special viral reservoir host
Authors: M Ahn, DE Anderson, Q Zhang, CW Tan, BL Lim, K Luko, M Wen, WN Chia, S Mani, LC Wang, JHJ Ng, RM Sobota, CA Dutertre, F Ginhoux, ZL Shi, AT Irving, LF Wang
Nat Microbiol, 2019;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Engulfment, persistence and fate of Bdellovibrio bacteriovorus predators inside human phagocytic cells informs their future therapeutic potential
Authors: D Raghunatha, PM Radford, C Gell, D Negus, C Moore, R Till, PJ Tighe, SP Wheatley, L Martinez-P, RE Sockett, J Tyson
Sci Rep, 2019;9(1):4293.
Species: Human
Sample Types: Cell Culture Supernates
-
Crosstalk between cancer cells and tumor associated macrophages is required for mesenchymal circulating tumor cell-mediated colorectal cancer metastasis
Authors: C Wei, C Yang, S Wang, D Shi, C Zhang, X Lin, Q Liu, R Dou, B Xiong
Mol. Cancer, 2019;18(1):64.
Species: Human
Sample Types: Cell Culture Supernates
-
Phenotypic and functional stability of leukocytes from human peripheral blood samples: considerations for the design of immunological studies
Authors: A Navas, L Giraldo-Pa, MD Prieto, J Cabrera, MA Gómez
BMC Immunol., 2019;20(1):5.
Species: Human
Sample Types: Cell Culture Supernates
-
β-Glucan-Induced Trained Immunity Protects against Leishmania braziliensis Infection: a Crucial Role for IL-32
Authors: JC Dos Santos, AM Barroso de, MV Teodoro Si, B Cirovic, LCJ de Bree, MSMA Damen, SJCFM Moorlag, RS Gomes, MM Helsen, M Oosting, ST Keating, A Schlitzer, MG Netea, F Ribeiro-Di, LAB Joosten
Cell Rep, 2019;28(10):2659-2672.e6.
Species: Human
Sample Types: Cell Culture Supernates
-
Relative Contributions of Extracellular and Internalized Bacteria to Early Macrophage Proinflammatory Responses to Streptococcus pneumoniae
Authors: J Periselner, G Ercoli, T Pollard, S Chimalapat, E Camberlein, G Szylar, C Hyams, G Tomlinson, FC Petersen, RA Floto, M Noursadegh, JS Brown
MBio, 2019;10(5):.
Species: Human
Sample Types: Cell Culture Supernates
-
Inflammasome-Driven Interleukin-1α and�Interleukin-1? Production in Atherosclerotic Plaques Relates to Hyperlipidemia and Plaque Complexity
Authors: X Jiang, F Wang, Y Wang, A Gisterå, J Roy, G Paulsson-B, U Hedin, A Lerman, GK Hansson, J Herrmann, ZQ Yan
JACC Basic Transl Sci, 2019;4(3):304-317.
Species: Human
Sample Types: Tissue Culture Supernates
-
EASIX for prediction of survival in lower-risk myelodysplastic syndromes
Authors: A Merz, U Germing, G Kobbe, J Kaivers, A Jauch, A Radujkovic, M Hummel, A Benner, M Merz, P Dreger, T Luft
Blood Cancer J, 2019;9(11):85.
Species: Human
Sample Types: Plasma
-
Impact of constitutional TET2 haploinsufficiency on molecular and clinical phenotype in humans
Authors: E Kaasinen, O Kuismin, K Rajamäki, H Ristolaine, M Aavikko, J Kondelin, S Saarinen, DG Berta, R Katainen, EAM Hirvonen, A Karhu, A Taira, T Tanskanen, A Alkodsi, M Taipale, E Morgunova, K Franssila, R Lehtonen, M Mäkinen, K Aittomäki, A Palotie, MI Kurki, O Pietiläine, M Hilpert, E Saarentaus, J Niinimäki, J Junttila, K Kaikkonen, P Vahteristo, RC Skoda, MRJ Seppänen, KK Eklund, J Taipale, O Kilpivaara, LA Aaltonen
Nat Commun, 2019;10(1):1252.
Species: Human
Sample Types: Cell Culture Supernates
-
Enteropathogenic Escherichia coli Stimulates Effector-Driven Rapid Caspase-4 Activation in Human Macrophages
Authors: PJ Goddard, J Sanchez-Ga, SL Slater, M Kalyan, D Ruano-Gall, O Marchès, LÁ Fernández, G Frankel, AR Shenoy
Cell Rep, 2019;27(4):1008-1017.e6.
Species: Human
Sample Types: Cell Culture Supernates
-
The effect of compressive force combined with mechanical vibration on human alveolar bone osteoblasts
Authors: C Chatmahamo, A Pravithara, S Suttapreya, C Leethanaku
J Oral Biol Craniofac Res, 2019;9(1):81-85.
Species: Human
Sample Types: Cell Culture Supernates
-
Human metapneumovirus activates NOD-like receptor protein 3 inflammasome via its small hydrophobic protein which plays a detrimental role during infection in mice
Authors: VB Lê, J Dubois, C Couture, MH Cavanagh, O Uyar, A Pizzorno, M Rosa-Calat, MÈ Hamelin, G Boivin
PLoS Pathog., 2019;15(4):e1007689.
Species: Human
Sample Types: Cell Culture Supernates
-
Expression of SAA1, SAA2 and SAA4 genes in human primary monocytes and monocyte-derived macrophages
Authors: C Jumeau, F Awad, E Assrawi, L Cobret, P Duquesnoy, I Giurgea, D Valeyre, G Grateau, S Amselem, JF Bernaudin, SA Karabina
PLoS ONE, 2019;14(5):e0217005.
Species: Human
Sample Types: Cell Culture Supernates
-
In Vitro Induction of T Helper 17 Cells by Synergistic Activation of Human Monocyte-Derived Langerhans Cell-Like Cells with Bacterial Agonists
Authors: R Gramlich, E Aliahmadi, M Peiser
Int J Mol Sci, 2019;20(6):.
Species: Human
Sample Types: Cell Culture Supernates
-
Regulation of hemolysin in uropathogenic Escherichia coli fine-tunes killing of human macrophages
Authors: AMV Murthy, MD Phan, KM Peters, NTK Nhu, RA Welch, GC Ulett, MA Schembri, MJ Sweet
Virulence, 2018;0(0):1-32.
Species: Human
Sample Types: Cell Culture Supernates
-
GWAS for Interleukin-1? levels in gingival crevicular fluid identifies IL37 variants in periodontal inflammation
Authors: S Offenbache, Y Jiao, SJ Kim, J Marchesan, KL Moss, L Jing, K Divaris, S Bencharit, CS Agler, T Morelli, S Zhang, L Sun, WT Seaman, D Cowley, SP Barros, JD Beck, M Munz, AS Schaefer, KE North
Nat Commun, 2018;9(1):3686.
Species: Human
Sample Types: Cell Culture Supernates
-
Saturated Fatty Acids Undergo Intracellular Crystallization and Activate the NLRP3 Inflammasome in Macrophages
Authors: T Karasawa, A Kawashima, F Usui-Kawan, S Watanabe, H Kimura, R Kamata, K Shirasuna, Y Koyama, A Sato-Tomit, T Matsuzaka, H Tomoda, SY Park, N Shibayama, H Shimano, T Kasahara, M Takahashi
Arterioscler. Thromb. Vasc. Biol., 2018;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Clinical predictors of liver fibrosis in patients with chronic hepatitis B virus infection from children to adults
Authors: JF Wu, SH Song, CS Lee, HL Chen, YH Ni, HY Hsu, TC Wu, MH Chang
J. Infect. Dis., 2018;0(0):.
Species: Human
Sample Types: Serum
-
Association of genetic variations in PTPN2 and CD122 with ocular Behcet's disease
Authors: Q Zhang, H Li, S Hou, H Yu, G Su, B Deng, J Qi, C Zhou, A Kijlstra, P Yang
Br J Ophthalmol, 2018;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
TiO2 nanoparticles can selectively bind CXCL8 impacting on neutrophil chemotaxis
Authors: J Batt, M Milward, I Chapple, M Grant, H Roberts, O Addison
Eur Cell Mater, 2018;35(0):13-24.
-
Association of Long Noncoding RNAs Polymorphisms With Ankylosing Spondylitis, Vogt-Koyanagi-Harada Disease, and Behcet's Disease
Authors: Y Yue, J Zhang, L Yang, S Liu, J Qi, Q Cao, C Zhou, Y Wang, A Kijlstra, P Yang, S Hou
Invest. Ophthalmol. Vis. Sci., 2018;59(2):1158-1166.
Species: Human
Sample Types: Cell Culture Supernates
-
Caspase-1 inhibition prevents glial inflammasome activation and pyroptosis in models of multiple sclerosis
Authors: BA McKenzie, MK Mamik, LB Saito, R Boghozian, MC Monaco, EO Major, JQ Lu, WG Branton, C Power
Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Human caspase-4 detects tetra-acylated LPS and cytosolic Francisella and functions differently from murine caspase-11
Authors: B Lagrange, S Benaoudia, P Wallet, F Magnotti, A Provost, F Michal, A Martin, F Di Lorenzo, BF Py, A Molinaro, T Henry
Nat Commun, 2018;9(1):242.
Species: Human
Sample Types: Cell Culture Supernates
-
Identification of genes required for Mycobacterium abscessus growth in vivo with a prominent role of the ESX-4 locus
Authors: L Laencina, V Dubois, V Le Moigne, A Viljoen, L Majlessi, J Pritchard, A Bernut, L Piel, AL Roux, JL Gaillard, B Lombard, D Loew, EJ Rubin, R Brosch, L Kremer, JL Herrmann, F Girard-Mis
Proc. Natl. Acad. Sci. U.S.A., 2018;115(5):E1002-E1011.
Species: Human
Sample Types: Cell Culture Supernates
-
The Effects of Varying Degree of MWCNT Carboxylation on Bioactivity in Various In Vivo and In Vitro Exposure Models
Authors: RF Hamilton, Z Wu, S Mitra, A Holian
Int J Mol Sci, 2018;19(2):.
Species: Human
Sample Types: Cell Culture Supernates
-
Respiratory hazard assessment of combined exposure to complete gasoline exhaust and respirable volcanic ash in a multicellular human lung model at the air-liquid interface
Authors: I Tomašek, CJ Horwell, C Bisig, DE Damby, P Comte, J Czerwinski, A Petri-Fink, MJD Clift, B Drasler, B Rothen-Rut
Environ. Pollut., 2018;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Annexin A1, FPR2/ALX, and inflammatory cytokine expression in peritoneal endometriosis
Authors: LK Volpato, VV Horewicz, F Bobinski, DF Martins, AP Piovezan
J. Reprod. Immunol., 2018;129(0):30-35.
Species: Human
Sample Types: Peritoneal Fluid
-
CD47 as a potential prognostic marker for oral leukoplakia and oral squamous cell carcinoma
Authors: X Ye, X Wang, R Lu, J Zhang, X Chen, G Zhou
Oncol Lett, 2018;15(6):9075-9080.
Species: Human
Sample Types: Cell Culture Supernates
-
Serum metabolomic profiling predicts synovial gene expression in rheumatoid arthritis
Authors: R Narasimhan, R Coras, SB Rosenthal, SR Sweeney, A Lodi, S Tiziani, D Boyle, A Kavanaugh, M Guma
Arthritis Res. Ther., 2018;20(1):164.
Species: Human
Sample Types: Serum
-
TET-2 up-regulation is associated with the anti-inflammatory action of Vicenin-2
Authors: N Hassan, A Ali, C Withycombe, M Ahluwalia, RH Al-Nasseri, A Tonks, K Morris
Cytokine, 2018;108(0):37-42.
Species: Human
Sample Types: Cell Culture Supernates
-
S100B regulates inflammatory response during osteoarthritis via fibroblast growth factor receptor 1 signaling
Authors: L Zhu, Z Weng, P Shen, J Zhou, J Zeng, F Weng, X Zhang, H Yang
Mol Med Rep, 2018;18(6):4855-4864.
Species: Human
Sample Types: Cell Culture Supernates
-
A low-gluten diet induces changes in the intestinal microbiome of healthy Danish adults
Authors: LBS Hansen, HM Roager, NB Søndertoft, RJ Gøbel, M Kristensen, M Vallès-Col, S Vieira-Sil, S Ibrügger, MV Lind, RB Mærkedahl, MI Bahl, ML Madsen, J Havelund, G Falony, I Tetens, T Nielsen, KH Allin, HL Frandsen, B Hartmann, JJ Holst, MH Sparholt, J Holck, A Blennow, JM Moll, AS Meyer, C Hoppe, JH Poulsen, V Carvalho, D Sagnelli, MD Dalgaard, AF Christense, MC Lydolph, AB Ross, S Villas-Bôa, S Brix, T Sicheritz-, K Buschard, A Linneberg, JJ Rumessen, CT Ekstrøm, C Ritz, K Kristianse, HB Nielsen, H Vestergaar, NJ Færgeman, J Raes, H Frøkiær, T Hansen, L Lauritzen, R Gupta, TR Licht, O Pedersen
Nat Commun, 2018;9(1):4630.
Species: Human
Sample Types: Cell Culture Supernates
-
A central role for P2X7 receptors in human microglia
Authors: L Janks, CVR Sharma, TM Egan
J Neuroinflammation, 2018;15(1):325.
Species: Human
Sample Types: Cell Culture Supernates
-
Combining Calcium Phosphates with Polysaccharides: A Bone-Inspired Material Modulating Monocyte/Macrophage Early Inflammatory Response
Authors: H Rammal, C Bour, M Dubus, L Entz, L Aubert, SC Gangloff, S Audonnet, NB Bercu, F Boulmedais, C Mauprivez, H Kerdjoudj
Int J Mol Sci, 2018;19(11):.
Species: Human
Sample Types: Cell Culture Supernates
-
A Transient Pseudosenescent Secretome Promotes Tumor Growth after Antiangiogenic Therapy Withdrawal
Authors: M Mastri, A Tracz, CR Lee, M Dolan, K Attwood, JG Christense, S Liu, JML Ebos
Cell Rep, 2018;25(13):3706-3720.e8.
Species: Human
Sample Types: Cell Culture Supernates
-
PDGF enhances the protective effect of adipose stem cell-derived extracellular vesicles in a model of acute hindlimb ischemia
Authors: T Lopatina, E Favaro, C Grange, M Cedrino, A Ranghino, S Occhipinti, S Fallo, F Buffolo, DA Gaykalova, MM Zanone, R Romagnoli, G Camussi
Sci Rep, 2018;8(1):17458.
Species: Human
Sample Types: Cell Culture Supernates
-
Sulphamethazine derivatives as immunomodulating agents: New therapeutic strategies for inflammatory diseases
Authors: H Siddiqui, HM Haniffa, A Jabeen, AU -Rahman, MI Choudhary
PLoS ONE, 2018;13(12):e0208933.
Species: Human
Sample Types: Cell Culture Supernates
-
Analysis of the association between Fc receptor family gene polymorphisms and ocular Beh�et's disease in Han Chinese
Authors: D Zhang, J Qin, L Li, G Su, G Huang, Q Cao, A Kijlstra, P Yang
Sci Rep, 2018;8(1):4850.
Species: Human
Sample Types: Cell Culture Supernates
-
The Viral Tegument Protein pp65 Impairs Transcriptional Upregulation of IL-1? by Human Cytomegalovirus through Inhibition of NF-kB Activity
Authors: M Biolatti, V Dell'Oste, S Scutera, F Gugliesi, G Griffante, M De Andrea, T Musso, S Landolfo
Viruses, 2018;10(10):.
Species: Human
Sample Types: Cell Culture Supernates
-
Complement receptor CD46 co-stimulates optimal human CD8+ T cell effector function via fatty acid metabolism
Authors: G Arbore, EE West, J Rahman, G Le Friec, N Niyonzima, M Pirooznia, I Tunc, P Pavlidis, N Powell, Y Li, P Liu, A Servais, L Couzi, V Fremeaux-B, L Placais, A Ferraro, PR Walsh, D Kavanagh, B Afzali, P Lavender, HJ Lachmann, C Kemper
Nat Commun, 2018;9(1):4186.
Species: Human
Sample Types: Cell Culture Supernates
-
Differential bronchial epithelial response regulated by ?Np63: a functional understanding of the epithelial shedding found in asthma
Authors: T Kubo, M Tsujiwaki, Y Hirohashi, T Tsukahara, T Kanaseki, M Nakatsugaw, T Hasegawa, T Torigoe
Lab. Invest., 2018;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Vaccine antigens modulate the innate response of monocytes to Al(OH)3
Authors: S Kooijman, J Brummelman, CACM van Els, F Marino, AJR Heck, E van Riet, B Metz, GFA Kersten, JLA Pennings, HD Meiring
PLoS ONE, 2018;13(5):e0197885.
Species: Human
Sample Types: Cell Culture Supernates
-
IL-18 associated with lung lymphoid aggregates drives IFN? production in severe COPD
Authors: E Briend, GJ Ferguson, M Mori, G Damera, K Stephenson, NA Karp, S Sethi, CK Ward, MA Sleeman, JS Erjefält, DK Finch
Respir. Res., 2017;18(1):159.
Species: Human
Sample Types: Cell Culture Supernates
-
Differential inhibition of activity, activation and gene expression of MMP-9 in THP-1 cells by azithromycin and minocycline versus bortezomib: A comparative study
Authors: J Vandooren, S Knoops, JL Aldinucci, L Boon, E Martens, G Opdenakker, E Kolaczkows
PLoS ONE, 2017;12(4):e0174853.
Species: Human
Sample Types: Cell Culture Supernates
-
Th17 micro-milieu regulates NLRP1-dependent caspase-5 activity in skin autoinflammation
Authors: S Zwicker, E Hattinger, D Bureik, A Batycka-Ba, A Schmidt, PA Gerber, S Rothenfuss, M Gilliet, T Ruzicka, R Wolf
PLoS ONE, 2017;12(4):e0175153.
Species: Human
Sample Types: Cell Culture Supernates
-
Rheumatoid arthritis bone marrow environment supports Th17 response.
Authors: Ewa Kuca-Warna, Weronika Kurowska, Monika Prochorec, Anna Radzikows, Tomasz Burakowsk, Urszula Skalska, Magdalena Massalska, Magdalena Pleba?czy, Barbara Ma?dyk-No, Iwona S?owi?ska, Robert Gasik, W?odzimierz Ma?li?ski
Arthritis Research & Therapy, 2017;0(0):1478-6362.
Species: Human
Sample Types: Plasma
-
Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
Authors: LM Scott, AH Bryant, A Rees, B Down, RH Jones, CA Thornton
Cytokine, 2017;0(0):.
Species: Human
Sample Types: Tissue Culture Supernates
-
In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens
Authors: N Goli?, K Veljovi?, N Popovi?, J Djoki?, I Strahini?, I Mrvaljevi?, A Terzi?-Vid
BMC Microbiol., 2017;17(1):108.
Species: Human
Sample Types: Cell Culture Supernates
-
Respiratory syncytial virus infection of newborn CX3CR1-deficent mice induces a pathogenic pulmonary innate immune response
Authors: S Das, M Raundhal, J Chen, TB Oriss, R Huff, JV Williams, A Ray, P Ray
JCI Insight, 2017;2(17):.
Species: Human
Sample Types: Cell Culture Supernates
-
Elevated CRP levels predict poor outcome and tumor recurrence in patients with thymic epithelial tumors: A pro- and retrospective analysis
Authors: S Janik, C Bekos, P Hacker, T Raunegger, B Ghanim, E Einwallner, L Beer, W Klepetko, L Müllauer, HJ Ankersmit, B Moser
Oncotarget, 2017;8(29):47090-47102.
Species: Human
Sample Types: Serum
-
TB lymphadenitis is associated with enhanced baseline and antigen - specific induction of Type 1 and Type 17 cytokines and reduced IL-1? and IL-18 at the site of infection
Authors: GR Kathamuthu, K Moideen, D Baskaran, VV Banurekha, D Nair, G Sekar, R Sridhar, B Vidyajayan, G Gajendrara, DK Parandhama, A Srinivasan, S Babu
Clin. Vaccine Immunol, 2017;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Inhibition of the inflammatory response to stress by targeting interaction between PKR and its cellular activator PACT
Authors: S Dabo, P Maillard, M Collados R, MD Hansen, S Mazouz, DJ Bigot, M Tible, G Janvier, O Helynck, P Cassonnet, Y Jacob, J Bellalou, A Gatignol, RC Patel, J Hugon, H Munier-Leh, EF Meurs
Sci Rep, 2017;7(1):16129.
Species: Human
Sample Types: Cell Culture Supernates
-
Rheumatoid arthritis bone marrow environment supports Th17 response
Authors: E Kuca-Warna, W Kurowska, M Prochorec-, A Radzikowsk, T Burakowski, U Skalska, M Massalska, M Pleba?czyk, B Ma?dyk-Now, I S?owi?ska, R Gasik, W Ma?li?ski
Arthritis Res. Ther., 2017;19(1):274.
Species: Human
Sample Types: Plasma
-
Vitamin D both facilitates and attenuates the cellular response to lipopolysaccharide
Authors: L Chen, MS Eapen, GR Zosky
Sci Rep, 2017;7(0):45172.
Species: Human
Sample Types: Cell Culture Supernates
-
In vitro effects of hyaluronic acid on human periodontal ligament cells
Authors: M Fujioka-Ko, HD Mller, A Mueller, A Lussi, A Sculean, PR Schmidlin, RJ Miron
BMC Oral Health, 2017;17(1):44.
Species: Human
Sample Types: Cell Culture Supernates
-
Active MLKL triggers the NLRP3 inflammasome in a cell-intrinsic manner
Authors: SA Conos, KW Chen, D De Nardo, H Hara, L Whitehead, G N£¤ez, SL Masters, JM Murphy, K Schroder, DL Vaux, KE Lawlor, LM Lindqvist, JE Vince
Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Effect of pre-emptive analgesia on clinical parameters and tissue levels of TNF-? and IL-1? in third molar surgery: a triple-blind, randomized, placebo-controlled study
Authors: AFM Albuquerqu, CSR Fonteles, DR do Val, HV Chaves, MM Bezerra, KMA Pereira, PG de Barros, BB de Lima, ECS Soares, TR Ribeiro, FWG Costa
Int J Oral Maxillofac Surg, 2017;0(0):.
Species: Human
Sample Types: Tissue Homogenates
-
Penicillinase-resistant antibiotics induce non-immune-mediated cholestasis through HSP27 activation associated with PKC/P38 and PI3K/AKT signaling pathways
Authors: A Burban, A Sharanek, R Hüe, M Gay, S Routier, A Guillouzo, C Guguen-Gui
Sci Rep, 2017;7(1):1815.
Species: Human
Sample Types: Cell Culture Supernates
-
Altered distribution of peripheral blood dendritic cell subsets in patients with pulmonary paracoccidioidomycosis
Authors: J Venturini, RS Cavalcante, DV Moris, M de Assis G, AD Levorato, KH Dos Reis, MSP de Arruda, RP Mendes
Acta Trop., 2017;0(0):.
Species: Human
Sample Types: Serum
-
Changes in peripheral immune cell numbers and functions in octogenarian walkers - an acute exercise study
Authors: KS van der Ge, Q Wang, TM Eijsvogels, HJ Koenen, I Joosten, E Brouwer, MT Hopman, JF Jacobs, AM Boots
Immun Ageing, 2017;14(0):5.
Species: Human
Sample Types: Cell Culture Supernates
-
Induction of immune gene expression and inflammatory mediator release by commonly used surgical suture materials: an experimental in vitro study
Authors: AM Lock, R Gao, D Naot, B Coleman, J Cornish, DS Musson
Patient Saf Surg, 2017;11(0):16.
Species: Human
Sample Types: Cell Culture Supernates
-
Iron nanomedicines induce Toll-like receptor activation, cytokine production and complement activation
Authors: Johan J F Verhoef
Biomaterials, 2017;119(0):68-77.
Species: Human
Sample Types: Cell Culture Supernates
-
An adaptive signaling network in melanoma inflammatory niches confers tolerance to MAPK signaling inhibition
Authors: HL Young, EJ Rowling, M Bugatti, E Giurisato, N Luheshi, I Arozarena, JC Acosta, J Kamarashev, DT Frederick, ZA Cooper, A Reuben, J Gil, KT Flaherty, JA Wargo, W Vermi, MP Smith, C Wellbrock, A Hurlstone
J. Exp. Med., 2017;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Genetic polymorphisms of cell adhesion molecules in Behcet's disease in a Chinese Han population
Authors: M Zheng, L Zhang, H Yu, J Hu, Q Cao, G Huang, Y Huang, G Yuan, A Kijlstra, P Yang
Sci Rep, 2016;6(0):24974.
Species: Human
Sample Types: Cell Culture Supernates
-
Characterization of the MMP/TIMP Imbalance and Collagen Production Induced by IL-1? or TNF-? Release from Human Hepatic Stellate Cells
Authors: S Robert, T Gicquel, A Bodin, V Lagente, E Boichot
PLoS ONE, 2016;11(4):e0153118.
Species: Human
Sample Types: Cell Culture Supernates
-
Combined exposure of diesel exhaust particles and respirable Soufri�re Hills volcanic ash causes a (pro-)inflammatory response in an in vitro multicellular epithelial tissue barrier model
Authors: Ines Tomašek
Part Fibre Toxicol, 2016;13(1):67.
Species: Human
Sample Types: Cell Culture Supernates
-
Immune-Complexed Adenovirus Induce AIM2-Mediated Pyroptosis in Human Dendritic Cells
PLoS Pathog, 2016;12(9):e1005871.
Species: Human
Sample Types: Cell Culture Supernates
-
Modulating endotoxin activity by combinatorial bioengineering of meningococcal lipopolysaccharide
Sci Rep, 2016;6(0):36575.
Species: Human
Sample Types: Cell Culture Supernates
-
Stromal fibroblasts support dendritic cells to maintain IL-23/Th17 responses after exposure to ionizing radiation
Authors: A Malecka, Q Wang, S Shah, RV Sutavani, I Spendlove, JM Ramage, J Greensmith, HA Franks, MJ Gough, A Saalbach, PM Patel, AM Jackson
J Leukoc Biol, 2016;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
CD4+ T Cells Recognizing PE/PPE Antigens Directly or via Cross Reactivity Are Protective against Pulmonary Mycobacterium tuberculosis Infection
PLoS Pathog, 2016;12(7):e1005770.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Reduced systemic and mycobacterial antigen-stimulated concentrations of IL-1? and IL-18 in tuberculous lymphadenitis
Cytokine, 2016;90(0):66-72.
Species: Human
Sample Types: Cell Culture Supernates
-
Dental Calculus Stimulates Interleukin-1? Secretion by Activating NLRP3 Inflammasome in Human and Mouse Phagocytes
Authors: JL Montenegro, A Yoshimura, Z Sm, T Kaneko, Y Ozaki, T Ukai, T Miyazaki, E Latz, Y Hara
PLoS ONE, 2016;11(9):e0162865.
Species: Human
Sample Types: Cell Culture Supernates
-
Dual Inhibition of Rip2 and IRAK1/4 Regulates IL-1? and IL-6 in Sarcoidosis Alveolar Macrophages and Peripheral Blood Mononuclear Cells
J Immunol, 2016;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Analysis of receptor tyrosine kinase genetics identifies two novel risk loci in GAS6 and PROS1 in Beh�et's disease
Sci Rep, 2016;6(0):26662.
Species: Human
Sample Types: Cell Culture Supernates
-
Differential IL-1? secretion by monocyte subsets is regulated by Hsp27 through modulating mRNA stability
Sci Rep, 2016;6(0):39035.
Species: Human
Sample Types: Cell Culture Supernates
-
17-oxo-DHA displays additive anti-inflammatory effects with fluticasone propionate and inhibits the NLRP3 inflammasome
Sci Rep, 2016;6(0):37625.
Species: Human
Sample Types: Cell Culture Supernates
-
Interaction among activated lymphocytes and mesenchymal cells through podoplanin is critical for a high IL-17 secretion
Authors: Mélissa Noack
Arthritis Res Ther, 2016;18(0):148.
Species: Human
Sample Types: Cell Culture Supernates
-
Silk-based blood stabilization for diagnostics
Proc Natl Acad Sci USA, 2016;113(21):5892-7.
Species: Human
Sample Types: Serum
-
Biocompatibility of primers and an adhesive used for implant-retained maxillofacial prostheses: An in�vitro analysis
Authors: Daniela Micheline Dos Santos
J Prosthet Dent, 2016;0(0):.
-
Receptor-Type Protein-Tyrosine Phosphatase ? and Colony Stimulating Factor-1 Receptor in the Intestine: Cellular Expression and Cytokine- and Chemokine Responses by Interleukin-34 and Colony Stimulating Factor-1
PLoS ONE, 2016;11(11):e0167324.
Species: Human
Sample Types: Cell Culture Supernates
-
Interleukin-17A Induces IL-1? Secretion From RPE Cells Via the NLRP3 Inflammasome
Authors: S Zhang, N Yu, R Zhang, S Zhang, J Wu
Invest. Ophthalmol. Vis. Sci, 2016;57(2):312-9.
Species: Human
Sample Types: Cell Culture Supernates
-
Engineered CAR T Cells Targeting the Cancer-Associated Tn-Glycoform of the Membrane Mucin MUC1 Control Adenocarcinoma
Authors: Avery D Posey
Immunity, 2016;44(6):1444-54.
Species: Human
Sample Types: Cell Culture Supernates
-
The Inflammasome and the Epidermal Growth Factor Receptor (EGFR) Are Involved in the Staphylococcus aureus-Mediated Induction of IL-1alpha and IL-1beta in Human Keratinocytes.
Authors: Simanski M, Rademacher F, Schroder L, Glaser R, Harder J
PLoS ONE, 2016;11(1):e0147118.
Species: Human
Sample Types: Cell Culture Supernates
-
DNA Sensing via TLR-9 Constitutes a Major Innate Immunity Pathway Activated during Erythema Nodosum Leprosum
J Immunol, 2016;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Activated monocytes resist elimination by retinal pigment epithelium and downregulate their OTX2 expression via TNF-?
Aging Cell, 2016;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Graphene-augmented nanofiber scaffolds demonstrate new features in cells behaviour
Sci Rep, 2016;6(0):30150.
Species: Human
Sample Types: Cell Culture Supernates
-
Macrophage activation in acute exacerbation of idiopathic pulmonary fibrosis.
Authors: Schupp J, Binder H, Jager B, Cillis G, Zissel G, Muller-Quernheim J, Prasse A
PLoS ONE, 2015;10(1):e0116775.
Species: Human
Sample Types: BALF
-
Caspase-8 as an Effector and Regulator of NLRP3 Inflammasome Signaling.
Authors: Antonopoulos C, Russo H, El Sanadi C, Martin B, Li X, Kaiser W, Mocarski E, Dubyak G
J Biol Chem, 2015;290(33):20167-84.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women.
Authors: McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K
PLoS ONE, 2015;10(8):e0134619.
Species: Human
Sample Types: Plasma
-
Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways.
Authors: Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D
J Immunol, 2015;195(6):2818-28.
Species: Human
Sample Types: Cell Culture Supernates
-
In vitro progesterone modulation on bacterial endotoxin-induced production of IL-1beta, TNFalpha, IL-6, IL-8, IL-10, MIP-1alpha, and MMP-9 in pre-labor human term placenta.
Authors: Garcia-Ruiz G, Flores-Espinosa P, Preciado-Martinez E, Bermejo-Martinez L, Espejel-Nunez A, Estrada-Gutierrez G, Maida-Claros R, Flores-Pliego A, Zaga-Clavellina V
Reprod Biol Endocrinol, 2015;13(0):115.
Species: Human
Sample Types: Cell Culture Supernates
-
SRSF1 facilitates cytosolic DNA-induced production of type I interferons recognized by RIG-I.
Authors: Xue F, Li X, Zhao X, Wang L, Liu M, Shi R, Zheng J
PLoS ONE, 2015;10(2):e0115354.
Species: Human
Sample Types: Cell Culture Supernates
-
Transforming Growth Factor-beta and Interleukin-1beta Signaling Pathways Converge on the Chemokine CCL20 Promoter.
Authors: Brand O, Somanath S, Moermans C, Yanagisawa H, Hashimoto M, Cambier S, Markovics J, Bondesson A, Hill A, Jablons D, Wolters P, Lou J, Marks J, Baron J, Nishimura S
J Biol Chem, 2015;290(23):14717-28.
Species: Human
Sample Types: Tissue Homogenates
-
Neutrophil IL-1beta processing induced by pneumolysin is mediated by the NLRP3/ASC inflammasome and caspase-1 activation and is dependent on K+ efflux.
Authors: Karmakar M, Katsnelson M, Malak H, Greene N, Howell S, Hise A, Camilli A, Kadioglu A, Dubyak G, Pearlman E
J Immunol, 2015;194(4):1763-75.
Species: Human
Sample Types: Cell Culture Supernates
-
LPS-Stimulated Whole Blood Cytokine Production Is Not Related to Disease Behavior in Patients with Quiescent Crohn's Disease.
Authors: Broekman M, Roelofs H, Hoentjen F, Wiegertjes R, Stoel N, Joosten L, de Jong D, Wanten G
PLoS ONE, 2015;10(7):e0133932.
Species: Human
Sample Types: Plasma
-
Angiopoietin-1 upregulates de novo expression of IL-1beta and Il1-Ra, and the exclusive release of Il1-Ra from human neutrophils.
Authors: Haddad L, Sirois M
PLoS ONE, 2014;9(2):e88980.
Species: Human
Sample Types: Cell Lysates
-
Identification of the flagellin glycosylation system in Burkholderia cenocepacia and the contribution of glycosylated flagellin to evasion of human innate immune responses.
Authors: Hanuszkiewicz A, Pittock P, Humphries F, Moll H, Rosales A, Molinaro A, Moynagh P, Lajoie G, Valvano M
J Biol Chem, 2014;289(27):19231-44.
Species: Human
Sample Types: Cell Culture Supernates
-
Integrin alphaDbeta2 (CD11d/CD18) is expressed by human circulating and tissue myeloid leukocytes and mediates inflammatory signaling.
Authors: Miyazaki Y, Vieira-de-Abreu A, Harris E, Shah A, Weyrich A, Castro-Faria-Neto H, Zimmerman G
PLoS ONE, 2014;9(11):e112770.
Species: Human
Sample Types: Cell Culture Supernates
-
TLR ligands stimulation protects MSC from NK killing.
Authors: Giuliani M, Bennaceur-Griscelli A, Nanbakhsh A, Oudrhiri N, Chouaib S, Azzarone B, Durrbach A, Lataillade J
Stem Cells, 2014;32(1):290-300.
Species: Human
Sample Types: Cell Culture Supernates
-
Residual endotoxin contaminations in recombinant proteins are sufficient to activate human CD1c+ dendritic cells.
Authors: Schwarz H, Schmittner M, Duschl A, Horejs-Hoeck J
PLoS ONE, 2014;9(12):e113840.
Species: Human
Sample Types: Cell Culture Supernates
-
The small GTPase Arf6 is essential for the Tram/Trif pathway in TLR4 signaling.
Authors: Van Acker T, Eyckerman S, Vande Walle L, Gerlo S, Goethals M, Lamkanfi M, Bovijn C, Tavernier J, Peelman F
J Biol Chem, 2014;289(3):1364-76.
-
The effect of size on Ag nanosphere toxicity in macrophage cell models and lung epithelial cell lines is dependent on particle dissolution.
Authors: Hamilton, Raymond, Buckingham, Sarah, Holian, Andrij
Int J Mol Sci, 2014;15(4):6815-30.
Species: Human
Sample Types: Cell Culture Supernates
-
Neisseria gonorrhoeae modulates iron-limiting innate immune defenses in macrophages.
Authors: Zughaier, Susu M, Kandler, Justin L, Shafer, William
PLoS ONE, 2014;9(1):e87688.
Species: Human
Sample Types: Cell Culture Supernates
-
Activin A as a mediator of NK-dendritic cell functional interactions.
Authors: Seeger P, Bosisio D, Parolini S, Badolato R, Gismondi A, Santoni A, Sozzani S
J Immunol, 2014;192(3):1241-8.
Species: Human
Sample Types: Cell Culture Supernates
-
Kineret(R)/IL-1ra blocks the IL-1/IL-8 inflammatory cascade during recombinant Panton Valentine Leukocidin-triggered pneumonia but not during S. aureus infection.
Authors: Labrousse D, Perret M, Hayez D, da Silva S, Badiou C, Couzon F, Bes M, Chavanet P, Lina G, Vandenesch F, Croisier-Bertin D, Henry T
PLoS ONE, 2014;9(6):e97546.
-
A functional variant of PTPN22 confers risk for Vogt-Koyanagi-Harada syndrome but not for ankylosing spondylitis.
Authors: Zhang Q, Qi J, Hou S, DU L, Yu H, Cao Q, Zhou Y, Liao D, Kijlstra A, Yang P
PLoS ONE, 2014;9(5):e96943.
Species: Human
Sample Types: Cell Culture Supernates
-
Ex vivo cytokine release and pattern recognition receptor expression of subjects exposed to dampness: pilot study to assess the outcome of mould exposure to the innate immune system.
Authors: Punsmann S, Liebers V, Lotz A, Bruning T, Raulf M
PLoS ONE, 2013;8(12):e82734.
Species: Human
Sample Types: Cell Culture Supernates
-
Leptin enhances the secretion of interleukin (IL)-18, but not IL-1beta, from human monocytes via activation of caspase-1.
Authors: Jitprasertwong P, Jaedicke K, Nile C, Preshaw P, Taylor J
Cytokine, 2013;65(2):222-30.
Species: Human
Sample Types: Cell Culture Supernates
-
Association of TLR2 gene polymorphisms with ocular Behcet's disease in a Chinese Han population.
Authors: Fang J, Hu R, Hou S, Ye Z, Xiang Q, Qi J, Zhou Y, Kijlstra A, Yang P
Invest Ophthalmol Vis Sci, 2013;54(13):8384-92.
Species: Human
Sample Types: Cell Culture Supernates
-
Inflammasome induction in Rasmussen's encephalitis: cortical and associated white matter pathogenesis.
Authors: Ramaswamy V, Walsh J, Sinclair D, Johnson E, Tang-Wai R, Wheatley B, Branton W, Maingat F, Snyder T, Gross D, Power C
J Neuroinflammation, 2013;10(0):152.
Species: Human
Sample Types: Cell Culture Supernates
-
S100A12 suppresses pro-inflammatory, but not pro-thrombotic functions of serum amyloid A.
Authors: Chung Y, Goyette J, Tedla N, Hsu K, Geczy C
PLoS ONE, 2013;8(4):e62372.
-
Regulation of progranulin expression in human microglia and proteolysis of progranulin by matrix metalloproteinase-12 (MMP-12).
Authors: Suh HS, Choi N, Tarassishin L, Lee SC
PLoS ONE, 2012;7(4):e35115.
Species: Human
Sample Types: Cell Culture Supernates
-
Impact of vitamin D supplementation on markers of inflammation in adults with cystic fibrosis hospitalized for a pulmonary exacerbation.
Eur J Clin Nutr, 2012;66(9):1072-4.
Species: Human
Sample Types: Serum
-
Interleukin-1alpha controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33.
J. Exp. Med., 2012;209(8):1505-17.
Species: Human
Sample Types: Cell Culture Supernates
-
Monocytic microparticles activate endothelial cells in an IL-1{beta}-dependent manner.
Authors: Wang JG, Williams JC, Davis BK, Jacobson K, Doerschuk CM, Ting JP, Mackman N
Blood, 2011;118(8):2366-74.
Species: Human
Sample Types: Cell Lysates
-
Interleukin (IL)-31 induces pro-inflammatory cytokines in human monocytes and macrophages following stimulation with staphylococcal exotoxins.
Authors: Kasraie S, Niebuhr M, Werfel T
Allergy, 2010;65(6):712-21.
Species: Human
Sample Types: Cell Culture Supernates
-
Maternal IL-1beta production prevents lung injury in a mouse model of bronchopulmonary dysplasia.
Authors: Backstrom E, Lappalainen U, Bry K
Am. J. Respir. Cell Mol. Biol., 2010;42(2):149-60.
Species: Human
Sample Types: Tissue Homogenates
-
Reactive oxygen species-independent activation of the IL-1beta inflammasome in cells from patients with chronic granulomatous disease.
Authors: van de Veerdonk FL, Smeekens SP, Joosten LA, Kullberg BJ, Dinarello CA, Van der Meer JW, Netea MG
Proc. Natl. Acad. Sci. U.S.A., 2010;107(7):3030-3.
Species: Human
Sample Types: Cell Culture Supernates
-
Helminth antigens modulate immune responses in cells from multiple sclerosis patients through TLR2-dependent mechanisms.
Authors: Correale J, Farez M
J. Immunol., 2009;183(9):5999-6012.
Species: Human
Sample Types: Cell Culture Supernates
-
Matrix metalloproteinase-9 deficiency worsens lung injury in a model of bronchopulmonary dysplasia.
Authors: Lukkarinen H, Hogmalm A, Lappalainen U, Bry K
Am. J. Respir. Cell Mol. Biol., 2009;41(1):59-68.
Species: Human
Sample Types: Tissue Homogenates
-
The CDK domain of p21 is a suppressor of IL-1beta-mediated inflammation in activated macrophages.
Authors: Scatizzi JC, Mavers M, Hutcheson J, Young B, Shi B, Pope RM, Ruderman EM, Samways DS, Corbett JA, Egan TM, Perlman H
Eur. J. Immunol., 2009;39(3):820-5.
Species: Human
Sample Types: Cell Culture Supernates
-
TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
Authors: Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M
Eur. J. Immunol., 2009;39(5):1221-30.
Species: Human
Sample Types: Cell Culture Supernates
-
Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
Circulation, 2009;119(10):1424-32.
Species: Human
Sample Types: Plasma
-
Divergent effects of hypoxia on dendritic cell functions.
Authors: Mancino A, Schioppa T, Larghi P, Pasqualini F, Nebuloni M, Chen IH, Sozzani S, Austyn JM, Mantovani A, Sica A
Blood, 2008;112(9):3723-34.
Species: Human
Sample Types: Cell Culture Supernates
-
Impact of tobacco-smoke on key signaling pathways in the innate immune response in lung macrophages.
Authors: Birrell MA, Wong S, Catley MC, Belvisi MG
J. Cell. Physiol., 2008;214(1):27-37.
Species: Human
Sample Types: Cell Culture Supernates
-
Novel role for the liver X nuclear receptor in the suppression of lung inflammatory responses.
Authors: Birrell MA, Catley MC, Hardaker E, Wong S, Willson TM, McCluskie K, Leonard T, Farrow SN, Collins JL, Haj-Yahia S, Belvisi MG
J. Biol. Chem., 2007;282(44):31882-90.
Species: Human
Sample Types: Cell Culture Supernates
-
Early cytokine responses after percutaneous magnetic resonance imaging guided laser thermoablation of malignant liver tumors.
Authors: Kallio R, Sequeiros R, Surcel HM, Ohtonen P, Kiviniemi H, Syrjala H
Cytokine, 2007;34(5):278-83.
Species: Human
Sample Types: Serum
-
Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling.
Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M
J. Clin. Invest., 2007;117(12):3664-3672.
Species: Human
Sample Types: Cell Culture Supernates
-
Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
Blood, 2007;110(13):4319-30.
Species: Human
Sample Types: Cell Culture Supernates
-
Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.
Authors: Wu T, Xie C, Wang HW, Zhou XJ, Schwartz N, Calixto S, Mackay M, Aranow C, Putterman C, Mohan C
J. Immunol., 2007;179(10):7166-75.
Species: Human
Sample Types: Urine
-
Role of matrix metalloproteinases in the inflammatory response in human airway cell-based assays and in rodent models of airway disease.
Authors: Birrell MA, Wong S, Dekkak A, De Alba J, Haj-Yahia S, Belvisi MG
J. Pharmacol. Exp. Ther., 2006;318(2):741-50.
Species: Human
Sample Types: Cell Culture Supernates
-
B and T lymphocytes are the primary sources of RANKL in the bone resorptive lesion of periodontal disease.
Authors: Kawai T, Matsuyama T, Hosokawa Y, Makihira S, Seki M, Karimbux NY, Goncalves RB, Valverde P, Dibart S, Li YP, Miranda LA, Ernst CW, Izumi Y, Taubman MA
Am. J. Pathol., 2006;169(3):987-98.
Species: Human
Sample Types: Tissue Homogenates
-
Transmission of Mycobacterium tuberculosis undetected by tuberculin skin testing.
Authors: Anderson ST, Williams AJ, Brown JR, Newton SM, Simsova M, Nicol MP, Sebo P, Levin M, Wilkinson RJ, Wilkinson KA
Am. J. Respir. Crit. Care Med., 2006;173(9):1038-42.
Species: Human
Sample Types: Cell Culture Supernates
-
Increased ENA-78 in the follicular fluid of patients with endometriosis.
Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
Acta Obstet Gynecol Scand, 2006;85(3):336-42.
Species: Human
Sample Types: Follicular Fluid
-
Sepsis and pathophysiology of anthrax in a nonhuman primate model.
Authors: Stearns-Kurosawa DJ, Lupu F, Taylor FB, Kinasewitz G, Kurosawa S
Am. J. Pathol., 2006;169(2):433-44.
Species: Primate - Papio cynocephalus (Yellow Baboon)
Sample Types: Plasma
-
Synergistic stimulation of human monocytes and dendritic cells by Toll-like receptor 4 and NOD1- and NOD2-activating agonists.
Authors: Fritz JH, Girardin SE, Fitting C, Werts C, Mengin-Lecreulx D, Caroff M, Cavaillon JM, Philpott DJ, Adib-Conquy M
Eur. J. Immunol., 2005;35(8):2459-70.
Species: Human
Sample Types: Cell Culture Supernates
-
Adherence influences monocyte responsiveness to interleukin-10.
Authors: Petit-Bertron AF, Fitting C, Cavaillon JM, Adib-Conquy M
J. Leukoc. Biol., 2003;73(1):145-54.
Species: Human
Sample Types: Cell Culture Supernates
-
Differential effect of serotonin on cytokine production in lipopolysaccharide-stimulated human peripheral blood mononuclear cells: involvement of 5-hydroxytryptamine2A receptors.
Authors: Cloez-Tayarani I, Petit-Bertron AF, Venters HD, Cavaillon JM
Int. Immunol., 2003;15(2):233-40.
Species: Human
Sample Types: Cell Culture Supernates
-
IL-1, IL-6 and TNF-alpha concentrations in the peritoneal fluid of women with pelvic adhesions.
Authors: Cheong YC, Shelton JB, Laird SM, Richmond M, Kudesia G, Li TC, Ledger WL
Hum. Reprod., 2002;17(1):69-75.
Species: Human
Sample Types: Peritoneal Fluid
-
Comparison of inflammatory elements in nasal lavage and induced sputum following occupational exposure to moldy-building microbes.
Authors: Purokivi M, Hirvonen MR, Roponen M, Randell J, Vahteristo M, Tukiainen H
Inhal Toxicol, 2002;14(6):653-62.
Species: Human
Sample Types: Sputum
-
The correlation of adhesions and peritoneal fluid cytokine concentrations: a pilot study.
Authors: Cheong YC, Laird SM, Shelton JB, Ledger WL, Li TC, Cooke ID
Hum. Reprod., 2002;17(4):1039-45.
Species: Human
Sample Types: Peritoneal Fluid
-
Inflammatory mediators in nasal lavage, induced sputum and serum of employees with rheumatic and respiratory disorders.
Authors: Roponen M, Kiviranta J, Seuri M, Tukiainen H, Myllykangas-Luosujarvi R, Hirvonen MR
Eur. Respir. J., 2001;18(3):542-8.
Species: Human
Sample Types: Serum
FAQs
No product specific FAQs exist for this product, however you may
View all ELISA FAQsDuoSet Ancillary Reagent Kits
Supplemental ELISA Products
Reviews for Human IL-1 beta/IL-1F2 DuoSet ELISA
Average Rating: 4.7 (Based on 23 Reviews)
Have you used Human IL-1 beta/IL-1F2 DuoSet ELISA?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
THP-1 cells were incubated with 0.1-10ug/ml PAM3CSK4 for 24-hours and culture supernatant was collected. IL-1b standard was fit to a sigmoidal 4-paramater logistic curve. Max IL-1b production was found at the 1ug/ml dosage.
3D ciliated airway tissues (cat.# 502-3D-24) were from Cell Applications (San Diego, CA) and were cultured in maintenance medium (cat.# 511M-3D-100, Cell Applications).
Twenty four hours after pre-incubation with test substances, tissues were exposed to 1µg/ml endotoxin LPS and IL-1b was was quantified after 48 additional hours. Worked very well.
Detection of IL-1b secreted by human CAR-T cells engineered to express IL-1b (PMC658), compared to normal CAR-T cells (PMC650).
Not compatible with all substrates.