Human IL-1 beta/IL-1F2 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY201
DY201-05
Ancillary Products Available
Human IL-1 beta / IL-1F2 ELISA Standard Curve
1 Image
Product Details
Procedure
Citations (113)
FAQs
Supplemental Products
Reviews (12)

Human IL-1 beta/IL-1F2 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IL-1 beta/IL-1F2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

 

  

Data Example

Human IL-1 beta / IL-1F2 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 beta/IL-1F2

IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.

Long Name:
Interleukin 1 beta
Entrez Gene IDs:
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names:
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

 

Citations for Human IL-1 beta/IL-1F2 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

113 Citations: Showing 1 - 10
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  1. Differential bronchial epithelial response regulated by ?Np63: a functional understanding of the epithelial shedding found in asthma
    Authors: T Kubo, M Tsujiwaki, Y Hirohashi, T Tsukahara, T Kanaseki, M Nakatsugaw, T Hasegawa, T Torigoe
    Lab. Invest., 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. TiO2 nanoparticles can selectively bind CXCL8 impacting on neutrophil chemotaxis
    Authors: J Batt, M Milward, I Chapple, M Grant, H Roberts, O Addison
    Eur Cell Mater, 2018;35(0):13-24.
  3. The Effects of Varying Degree of MWCNT Carboxylation on Bioactivity in Various In Vivo and In Vitro Exposure Models
    Authors: RF Hamilton, Z Wu, S Mitra, A Holian
    Int J Mol Sci, 2018;19(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Analysis of the association between Fc receptor family gene polymorphisms and ocular Beh�et's disease in Han Chinese
    Authors: D Zhang, J Qin, L Li, G Su, G Huang, Q Cao, A Kijlstra, P Yang
    Sci Rep, 2018;8(1):4850.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Human caspase-4 detects tetra-acylated LPS and cytosolic Francisella and functions differently from murine caspase-11
    Authors: B Lagrange, S Benaoudia, P Wallet, F Magnotti, A Provost, F Michal, A Martin, F Di Lorenzo, BF Py, A Molinaro, T Henry
    Nat Commun, 2018;9(1):242.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. TET-2 up-regulation is associated with the anti-inflammatory action of Vicenin-2
    Authors: N Hassan, A Ali, C Withycombe, M Ahluwalia, RH Al-Nasseri, A Tonks, K Morris
    Cytokine, 2018;108(0):37-42.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Caspase-1 inhibition prevents glial inflammasome activation and pyroptosis in models of multiple sclerosis
    Authors: BA McKenzie, MK Mamik, LB Saito, R Boghozian, MC Monaco, EO Major, JQ Lu, WG Branton, C Power
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Identification of genes required for Mycobacterium abscessus growth in vivo with a prominent role of the ESX-4 locus
    Authors: L Laencina, V Dubois, V Le Moigne, A Viljoen, L Majlessi, J Pritchard, A Bernut, L Piel, AL Roux, JL Gaillard, B Lombard, D Loew, EJ Rubin, R Brosch, L Kremer, JL Herrmann, F Girard-Mis
    Proc. Natl. Acad. Sci. U.S.A., 2018;115(5):E1002-E1011.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Association of Long Noncoding RNAs Polymorphisms With Ankylosing Spondylitis, Vogt-Koyanagi-Harada Disease, and Behcet's Disease
    Authors: Y Yue, J Zhang, L Yang, S Liu, J Qi, Q Cao, C Zhou, Y Wang, A Kijlstra, P Yang, S Hou
    Invest. Ophthalmol. Vis. Sci., 2018;59(2):1158-1166.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Vaccine antigens modulate the innate response of monocytes to Al(OH)3
    Authors: S Kooijman, J Brummelman, CACM van Els, F Marino, AJR Heck, E van Riet, B Metz, GFA Kersten, JLA Pennings, HD Meiring
    PLoS ONE, 2018;13(5):e0197885.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Serum metabolomic profiling predicts synovial gene expression in rheumatoid arthritis
    Authors: R Narasimhan, R Coras, SB Rosenthal, SR Sweeney, A Lodi, S Tiziani, D Boyle, A Kavanaugh, M Guma
    Arthritis Res. Ther., 2018;20(1):164.
    Species: Human
    Sample Types: Serum
  12. Annexin A1, FPR2/ALX, and inflammatory cytokine expression in peritoneal endometriosis
    Authors: LK Volpato, VV Horewicz, F Bobinski, DF Martins, AP Piovezan
    J. Reprod. Immunol., 2018;129(0):30-35.
    Species: Human
    Sample Types: Peritoneal Fluid
  13. CD47 as a potential prognostic marker for oral leukoplakia and oral squamous cell carcinoma
    Authors: X Ye, X Wang, R Lu, J Zhang, X Chen, G Zhou
    Oncol Lett, 2018;15(6):9075-9080.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. GWAS for Interleukin-1? levels in gingival crevicular fluid identifies IL37 variants in periodontal inflammation
    Authors: S Offenbache, Y Jiao, SJ Kim, J Marchesan, KL Moss, L Jing, K Divaris, S Bencharit, CS Agler, T Morelli, S Zhang, L Sun, WT Seaman, D Cowley, SP Barros, JD Beck, M Munz, AS Schaefer, KE North
    Nat Commun, 2018;9(1):3686.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Inhibition of the inflammatory response to stress by targeting interaction between PKR and its cellular activator PACT
    Authors: S Dabo, P Maillard, M Collados R, MD Hansen, S Mazouz, DJ Bigot, M Tible, G Janvier, O Helynck, P Cassonnet, Y Jacob, J Bellalou, A Gatignol, RC Patel, J Hugon, H Munier-Leh, EF Meurs
    Sci Rep, 2017;7(1):16129.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens
    Authors: N Goli?, K Veljovi?, N Popovi?, J Djoki?, I Strahini?, I Mrvaljevi?, A Terzi?-Vid
    BMC Microbiol., 2017;17(1):108.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Induction of immune gene expression and inflammatory mediator release by commonly used surgical suture materials: an experimental in vitro study
    Authors: AM Lock, R Gao, D Naot, B Coleman, J Cornish, DS Musson
    Patient Saf Surg, 2017;11(0):16.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. TB lymphadenitis is associated with enhanced baseline and antigen - specific induction of Type 1 and Type 17 cytokines and reduced IL-1? and IL-18 at the site of infection
    Authors: GR Kathamuthu, K Moideen, D Baskaran, VV Banurekha, D Nair, G Sekar, R Sridhar, B Vidyajayan, G Gajendrara, DK Parandhama, A Srinivasan, S Babu
    Clin. Vaccine Immunol, 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Effect of pre-emptive analgesia on clinical parameters and tissue levels of TNF-? and IL-1? in third molar surgery: a triple-blind, randomized, placebo-controlled study
    Authors: AFM Albuquerqu, CSR Fonteles, DR do Val, HV Chaves, MM Bezerra, KMA Pereira, PG de Barros, BB de Lima, ECS Soares, TR Ribeiro, FWG Costa
    Int J Oral Maxillofac Surg, 2017;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
  20. Penicillinase-resistant antibiotics induce non-immune-mediated cholestasis through HSP27 activation associated with PKC/P38 and PI3K/AKT signaling pathways
    Authors: A Burban, A Sharanek, R Hüe, M Gay, S Routier, A Guillouzo, C Guguen-Gui
    Sci Rep, 2017;7(1):1815.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Changes in peripheral immune cell numbers and functions in octogenarian walkers - an acute exercise study
    Authors: KS van der Ge, Q Wang, TM Eijsvogels, HJ Koenen, I Joosten, E Brouwer, MT Hopman, JF Jacobs, AM Boots
    Immun Ageing, 2017;14(0):5.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Vitamin D both facilitates and attenuates the cellular response to lipopolysaccharide
    Authors: L Chen, MS Eapen, GR Zosky
    Sci Rep, 2017;7(0):45172.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Active MLKL triggers the NLRP3 inflammasome in a cell-intrinsic manner
    Authors: SA Conos, KW Chen, D De Nardo, H Hara, L Whitehead, G N£¤ez, SL Masters, JM Murphy, K Schroder, DL Vaux, KE Lawlor, LM Lindqvist, JE Vince
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Elevated CRP levels predict poor outcome and tumor recurrence in patients with thymic epithelial tumors: A pro- and retrospective analysis
    Authors: S Janik, C Bekos, P Hacker, T Raunegger, B Ghanim, E Einwallner, L Beer, W Klepetko, L Müllauer, HJ Ankersmit, B Moser
    Oncotarget, 2017;8(29):47090-47102.
    Species: Human
    Sample Types: Serum
  25. In vitro effects of hyaluronic acid on human periodontal ligament cells
    Authors: M Fujioka-Ko, HD Mller, A Mueller, A Lussi, A Sculean, PR Schmidlin, RJ Miron
    BMC Oral Health, 2017;17(1):44.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. An adaptive signaling network in melanoma inflammatory niches confers tolerance to MAPK signaling inhibition
    Authors: HL Young, EJ Rowling, M Bugatti, E Giurisato, N Luheshi, I Arozarena, JC Acosta, J Kamarashev, DT Frederick, ZA Cooper, A Reuben, J Gil, KT Flaherty, JA Wargo, W Vermi, MP Smith, C Wellbrock, A Hurlstone
    J. Exp. Med., 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Differential inhibition of activity, activation and gene expression of MMP-9 in THP-1 cells by azithromycin and minocycline versus bortezomib: A comparative study
    Authors: J Vandooren, S Knoops, JL Aldinucci, L Boon, E Martens, G Opdenakker, E Kolaczkows
    PLoS ONE, 2017;12(4):e0174853.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Rheumatoid arthritis bone marrow environment supports Th17 response
    Authors: E Kuca-Warna, W Kurowska, M Prochorec-, A Radzikowsk, T Burakowski, U Skalska, M Massalska, M Pleba?czyk, B Ma?dyk-Now, I S?owi?ska, R Gasik, W Ma?li?ski
    Arthritis Res. Ther., 2017;19(1):274.
    Species: Human
    Sample Types: Plasma
  29. Iron nanomedicines induce Toll-like receptor activation, cytokine production and complement activation
    Authors: Johan J F Verhoef
    Biomaterials, 2017;119(0):68-77.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
    Authors: LM Scott, AH Bryant, A Rees, B Down, RH Jones, CA Thornton
    Cytokine, 2017;0(0):.
    Species: Human
    Sample Types: Tissue Culture Supernates
  31. Altered distribution of peripheral blood dendritic cell subsets in patients with pulmonary paracoccidioidomycosis
    Authors: J Venturini, RS Cavalcante, DV Moris, M de Assis G, AD Levorato, KH Dos Reis, MSP de Arruda, RP Mendes
    Acta Trop., 2017;0(0):.
    Species: Human
    Sample Types: Serum
  32. Th17 micro-milieu regulates NLRP1-dependent caspase-5 activity in skin autoinflammation
    Authors: S Zwicker, E Hattinger, D Bureik, A Batycka-Ba, A Schmidt, PA Gerber, S Rothenfuss, M Gilliet, T Ruzicka, R Wolf
    PLoS ONE, 2017;12(4):e0175153.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Respiratory syncytial virus infection of newborn CX3CR1-deficent mice induces a pathogenic pulmonary innate immune response
    Authors: S Das, M Raundhal, J Chen, TB Oriss, R Huff, JV Williams, A Ray, P Ray
    JCI Insight, 2017;2(17):.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. IL-18 associated with lung lymphoid aggregates drives IFN? production in severe COPD
    Authors: E Briend, GJ Ferguson, M Mori, G Damera, K Stephenson, NA Karp, S Sethi, CK Ward, MA Sleeman, JS Erjefält, DK Finch
    Respir. Res., 2017;18(1):159.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Stromal fibroblasts support dendritic cells to maintain IL-23/Th17 responses after exposure to ionizing radiation
    Authors: A Malecka, Q Wang, S Shah, RV Sutavani, I Spendlove, JM Ramage, J Greensmith, HA Franks, MJ Gough, A Saalbach, PM Patel, AM Jackson
    J Leukoc Biol, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. Receptor-Type Protein-Tyrosine Phosphatase ? and Colony Stimulating Factor-1 Receptor in the Intestine: Cellular Expression and Cytokine- and Chemokine Responses by Interleukin-34 and Colony Stimulating Factor-1
    PLoS ONE, 2016;11(11):e0167324.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. Activated monocytes resist elimination by retinal pigment epithelium and downregulate their OTX2 expression via TNF-?
    Aging Cell, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. 17-oxo-DHA displays additive anti-inflammatory effects with fluticasone propionate and inhibits the NLRP3 inflammasome
    Sci Rep, 2016;6(0):37625.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. Engineered CAR T Cells Targeting the Cancer-Associated Tn-Glycoform of the Membrane Mucin MUC1 Control Adenocarcinoma
    Authors: Avery D Posey
    Immunity, 2016;44(6):1444-54.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. The Inflammasome and the Epidermal Growth Factor Receptor (EGFR) Are Involved in the Staphylococcus aureus-Mediated Induction of IL-1alpha and IL-1beta in Human Keratinocytes.
    Authors: Simanski M, Rademacher F, Schroder L, Glaser R, Harder J
    PLoS ONE, 2016;11(1):e0147118.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Interleukin-17A Induces IL-1? Secretion From RPE Cells Via the NLRP3 Inflammasome
    Authors: S Zhang, N Yu, R Zhang, S Zhang, J Wu
    Invest. Ophthalmol. Vis. Sci, 2016;57(2):312-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. Silk-based blood stabilization for diagnostics
    Proc Natl Acad Sci USA, 2016;113(21):5892-7.
    Species: Human
    Sample Types: Serum
  43. Genetic polymorphisms of cell adhesion molecules in Behcet's disease in a Chinese Han population
    Authors: M Zheng, L Zhang, H Yu, J Hu, Q Cao, G Huang, Y Huang, G Yuan, A Kijlstra, P Yang
    Sci Rep, 2016;6(0):24974.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Modulating endotoxin activity by combinatorial bioengineering of meningococcal lipopolysaccharide
    Sci Rep, 2016;6(0):36575.
    Species: Human
    Sample Types: Cell Culture Supernates
  45. CD4+ T Cells Recognizing PE/PPE Antigens Directly or via Cross Reactivity Are Protective against Pulmonary Mycobacterium tuberculosis Infection
    PLoS Pathog, 2016;12(7):e1005770.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  46. Graphene-augmented nanofiber scaffolds demonstrate new features in cells behaviour
    Sci Rep, 2016;6(0):30150.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. Interaction among activated lymphocytes and mesenchymal cells through podoplanin is critical for a high IL-17 secretion
    Authors: Mélissa Noack
    Arthritis Res Ther, 2016;18(0):148.
    Species: Human
    Sample Types: Cell Culture Supernates
  48. Combined exposure of diesel exhaust particles and respirable Soufri�re Hills volcanic ash causes a (pro-)inflammatory response in an in vitro multicellular epithelial tissue barrier model
    Authors: Ines Tomašek
    Part Fibre Toxicol, 2016;13(1):67.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. Immune-Complexed Adenovirus Induce AIM2-Mediated Pyroptosis in Human Dendritic Cells
    PLoS Pathog, 2016;12(9):e1005871.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. Differential IL-1? secretion by monocyte subsets is regulated by Hsp27 through modulating mRNA stability
    Sci Rep, 2016;6(0):39035.
    Species: Human
    Sample Types: Cell Culture Supernates
  51. Biocompatibility of primers and an adhesive used for implant-retained maxillofacial prostheses: An in�vitro analysis
    Authors: Daniela Micheline Dos Santos
    J Prosthet Dent, 2016;0(0):.
  52. Reduced systemic and mycobacterial antigen-stimulated concentrations of IL-1? and IL-18 in tuberculous lymphadenitis
    Cytokine, 2016;90(0):66-72.
    Species: Human
    Sample Types: Cell Culture Supernates
  53. DNA Sensing via TLR-9 Constitutes a Major Innate Immunity Pathway Activated during Erythema Nodosum Leprosum
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  54. Analysis of receptor tyrosine kinase genetics identifies two novel risk loci in GAS6 and PROS1 in Beh�et's disease
    Sci Rep, 2016;6(0):26662.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. Characterization of the MMP/TIMP Imbalance and Collagen Production Induced by IL-1? or TNF-? Release from Human Hepatic Stellate Cells
    Authors: S Robert, T Gicquel, A Bodin, V Lagente, E Boichot
    PLoS ONE, 2016;11(4):e0153118.
    Species: Human
    Sample Types: Cell Culture Supernates
  56. Dental Calculus Stimulates Interleukin-1? Secretion by Activating NLRP3 Inflammasome in Human and Mouse Phagocytes
    Authors: JL Montenegro, A Yoshimura, Z Sm, T Kaneko, Y Ozaki, T Ukai, T Miyazaki, E Latz, Y Hara
    PLoS ONE, 2016;11(9):e0162865.
    Species: Human
    Sample Types: Cell Culture Supernates
  57. Dual Inhibition of Rip2 and IRAK1/4 Regulates IL-1? and IL-6 in Sarcoidosis Alveolar Macrophages and Peripheral Blood Mononuclear Cells
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  58. LPS-Stimulated Whole Blood Cytokine Production Is Not Related to Disease Behavior in Patients with Quiescent Crohn's Disease.
    Authors: Broekman M, Roelofs H, Hoentjen F, Wiegertjes R, Stoel N, Joosten L, de Jong D, Wanten G
    PLoS ONE, 2015;10(7):e0133932.
    Species: Human
    Sample Types: Plasma
  59. Neutrophil IL-1beta processing induced by pneumolysin is mediated by the NLRP3/ASC inflammasome and caspase-1 activation and is dependent on K+ efflux.
    Authors: Karmakar M, Katsnelson M, Malak H, Greene N, Howell S, Hise A, Camilli A, Kadioglu A, Dubyak G, Pearlman E
    J Immunol, 2015;194(4):1763-75.
    Species: Human
    Sample Types: Cell Culture Supernates
  60. Caspase-8 as an Effector and Regulator of NLRP3 Inflammasome Signaling.
    Authors: Antonopoulos C, Russo H, El Sanadi C, Martin B, Li X, Kaiser W, Mocarski E, Dubyak G
    J Biol Chem, 2015;290(33):20167-84.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  61. Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women.
    Authors: McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K
    PLoS ONE, 2015;10(8):e0134619.
    Species: Human
    Sample Types: Plasma
  62. SRSF1 facilitates cytosolic DNA-induced production of type I interferons recognized by RIG-I.
    Authors: Xue F, Li X, Zhao X, Wang L, Liu M, Shi R, Zheng J
    PLoS ONE, 2015;10(2):e0115354.
    Species: Human
    Sample Types: Cell Culture Supernates
  63. Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways.
    Authors: Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D
    J Immunol, 2015;195(6):2818-28.
    Species: Human
    Sample Types: Cell Culture Supernates
  64. Transforming Growth Factor-beta and Interleukin-1beta Signaling Pathways Converge on the Chemokine CCL20 Promoter.
    Authors: Brand O, Somanath S, Moermans C, Yanagisawa H, Hashimoto M, Cambier S, Markovics J, Bondesson A, Hill A, Jablons D, Wolters P, Lou J, Marks J, Baron J, Nishimura S
    J Biol Chem, 2015;290(23):14717-28.
    Species: Human
    Sample Types: Tissue Homogenates
  65. Macrophage activation in acute exacerbation of idiopathic pulmonary fibrosis.
    Authors: Schupp J, Binder H, Jager B, Cillis G, Zissel G, Muller-Quernheim J, Prasse A
    PLoS ONE, 2015;10(1):e0116775.
    Species: Human
    Sample Types: BALF
  66. In vitro progesterone modulation on bacterial endotoxin-induced production of IL-1beta, TNFalpha, IL-6, IL-8, IL-10, MIP-1alpha, and MMP-9 in pre-labor human term placenta.
    Authors: Garcia-Ruiz G, Flores-Espinosa P, Preciado-Martinez E, Bermejo-Martinez L, Espejel-Nunez A, Estrada-Gutierrez G, Maida-Claros R, Flores-Pliego A, Zaga-Clavellina V
    Reprod Biol Endocrinol, 2015;13(0):115.
    Species: Human
    Sample Types: Cell Culture Supernates
  67. A functional variant of PTPN22 confers risk for Vogt-Koyanagi-Harada syndrome but not for ankylosing spondylitis.
    Authors: Zhang Q, Qi J, Hou S, DU L, Yu H, Cao Q, Zhou Y, Liao D, Kijlstra A, Yang P
    PLoS ONE, 2014;9(5):e96943.
    Species: Human
    Sample Types: Cell Culture Supernates
  68. Activin A as a mediator of NK-dendritic cell functional interactions.
    Authors: Seeger P, Bosisio D, Parolini S, Badolato R, Gismondi A, Santoni A, Sozzani S
    J Immunol, 2014;192(3):1241-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  69. Kineret(R)/IL-1ra blocks the IL-1/IL-8 inflammatory cascade during recombinant Panton Valentine Leukocidin-triggered pneumonia but not during S. aureus infection.
    Authors: Labrousse D, Perret M, Hayez D, da Silva S, Badiou C, Couzon F, Bes M, Chavanet P, Lina G, Vandenesch F, Croisier-Bertin D, Henry T
    PLoS ONE, 2014;9(6):e97546.
  70. The effect of size on Ag nanosphere toxicity in macrophage cell models and lung epithelial cell lines is dependent on particle dissolution.
    Authors: Hamilton, Raymond, Buckingham, Sarah, Holian, Andrij
    Int J Mol Sci, 2014;15(4):6815-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  71. Integrin alphaDbeta2 (CD11d/CD18) is expressed by human circulating and tissue myeloid leukocytes and mediates inflammatory signaling.
    Authors: Miyazaki Y, Vieira-de-Abreu A, Harris E, Shah A, Weyrich A, Castro-Faria-Neto H, Zimmerman G
    PLoS ONE, 2014;9(11):e112770.
    Species: Human
    Sample Types: Cell Culture Supernates
  72. TLR ligands stimulation protects MSC from NK killing.
    Authors: Giuliani M, Bennaceur-Griscelli A, Nanbakhsh A, Oudrhiri N, Chouaib S, Azzarone B, Durrbach A, Lataillade J
    Stem Cells, 2014;32(1):290-300.
    Species: Human
    Sample Types: Cell Culture Supernates
  73. Neisseria gonorrhoeae modulates iron-limiting innate immune defenses in macrophages.
    Authors: Zughaier, Susu M, Kandler, Justin L, Shafer, William
    PLoS ONE, 2014;9(1):e87688.
    Species: Human
    Sample Types: Cell Culture Supernates
  74. Residual endotoxin contaminations in recombinant proteins are sufficient to activate human CD1c+ dendritic cells.
    Authors: Schwarz H, Schmittner M, Duschl A, Horejs-Hoeck J
    PLoS ONE, 2014;9(12):e113840.
    Species: Human
    Sample Types: Cell Culture Supernates
  75. The small GTPase Arf6 is essential for the Tram/Trif pathway in TLR4 signaling.
    Authors: Van Acker T, Eyckerman S, Vande Walle L, Gerlo S, Goethals M, Lamkanfi M, Bovijn C, Tavernier J, Peelman F
    J Biol Chem, 2014;289(3):1364-76.
  76. Angiopoietin-1 upregulates de novo expression of IL-1beta and Il1-Ra, and the exclusive release of Il1-Ra from human neutrophils.
    Authors: Haddad L, Sirois M
    PLoS ONE, 2014;9(2):e88980.
    Species: Human
    Sample Types: Cell Lysates
  77. Identification of the flagellin glycosylation system in Burkholderia cenocepacia and the contribution of glycosylated flagellin to evasion of human innate immune responses.
    Authors: Hanuszkiewicz A, Pittock P, Humphries F, Moll H, Rosales A, Molinaro A, Moynagh P, Lajoie G, Valvano M
    J Biol Chem, 2014;289(27):19231-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  78. Leptin enhances the secretion of interleukin (IL)-18, but not IL-1beta, from human monocytes via activation of caspase-1.
    Authors: Jitprasertwong P, Jaedicke K, Nile C, Preshaw P, Taylor J
    Cytokine, 2013;65(2):222-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  79. Association of TLR2 gene polymorphisms with ocular Behcet's disease in a Chinese Han population.
    Authors: Fang J, Hu R, Hou S, Ye Z, Xiang Q, Qi J, Zhou Y, Kijlstra A, Yang P
    Invest Ophthalmol Vis Sci, 2013;54(13):8384-92.
    Species: Human
    Sample Types: Cell Culture Supernates
  80. Ex vivo cytokine release and pattern recognition receptor expression of subjects exposed to dampness: pilot study to assess the outcome of mould exposure to the innate immune system.
    Authors: Punsmann S, Liebers V, Lotz A, Bruning T, Raulf M
    PLoS ONE, 2013;8(12):e82734.
    Species: Human
    Sample Types: Cell Culture Supernates
  81. S100A12 suppresses pro-inflammatory, but not pro-thrombotic functions of serum amyloid A.
    Authors: Chung Y, Goyette J, Tedla N, Hsu K, Geczy C
    PLoS ONE, 2013;8(4):e62372.
  82. Inflammasome induction in Rasmussen's encephalitis: cortical and associated white matter pathogenesis.
    Authors: Ramaswamy V, Walsh J, Sinclair D, Johnson E, Tang-Wai R, Wheatley B, Branton W, Maingat F, Snyder T, Gross D, Power C
    J Neuroinflammation, 2013;10(0):152.
    Species: Human
    Sample Types: Cell Culture Supernates
  83. Impact of vitamin D supplementation on markers of inflammation in adults with cystic fibrosis hospitalized for a pulmonary exacerbation.
    Eur J Clin Nutr, 2012;66(9):1072-4.
    Species: Human
    Sample Types: Serum
  84. Regulation of progranulin expression in human microglia and proteolysis of progranulin by matrix metalloproteinase-12 (MMP-12).
    Authors: Suh HS, Choi N, Tarassishin L, Lee SC
    PLoS ONE, 2012;7(4):e35115.
    Species: Human
    Sample Types: Cell Culture Supernates
  85. Interleukin-1alpha controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33.
    J. Exp. Med., 2012;209(8):1505-17.
    Species: Human
    Sample Types: Cell Culture Supernates
  86. Monocytic microparticles activate endothelial cells in an IL-1{beta}-dependent manner.
    Authors: Wang JG, Williams JC, Davis BK, Jacobson K, Doerschuk CM, Ting JP, Mackman N
    Blood, 2011;118(8):2366-74.
    Species: Human
    Sample Types: Cell Lysates
  87. Maternal IL-1beta production prevents lung injury in a mouse model of bronchopulmonary dysplasia.
    Authors: Backstrom E, Lappalainen U, Bry K
    Am. J. Respir. Cell Mol. Biol., 2010;42(2):149-60.
    Species: Human
    Sample Types: Tissue Homogenates
  88. Interleukin (IL)-31 induces pro-inflammatory cytokines in human monocytes and macrophages following stimulation with staphylococcal exotoxins.
    Authors: Kasraie S, Niebuhr M, Werfel T
    Allergy, 2010;65(6):712-21.
    Species: Human
    Sample Types: Cell Culture Supernates
  89. Reactive oxygen species-independent activation of the IL-1beta inflammasome in cells from patients with chronic granulomatous disease.
    Authors: van de Veerdonk FL, Smeekens SP, Joosten LA, Kullberg BJ, Dinarello CA, Van der Meer JW, Netea MG
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(7):3030-3.
    Species: Human
    Sample Types: Cell Culture Supernates
  90. TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
    Authors: Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M
    Eur. J. Immunol., 2009;39(5):1221-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  91. Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
    Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
    Circulation, 2009;119(10):1424-32.
    Species: Human
    Sample Types: Plasma
  92. Matrix metalloproteinase-9 deficiency worsens lung injury in a model of bronchopulmonary dysplasia.
    Authors: Lukkarinen H, Hogmalm A, Lappalainen U, Bry K
    Am. J. Respir. Cell Mol. Biol., 2009;41(1):59-68.
    Species: Human
    Sample Types: Tissue Homogenates
  93. Helminth antigens modulate immune responses in cells from multiple sclerosis patients through TLR2-dependent mechanisms.
    Authors: Correale J, Farez M
    J. Immunol., 2009;183(9):5999-6012.
    Species: Human
    Sample Types: Cell Culture Supernates
  94. The CDK domain of p21 is a suppressor of IL-1beta-mediated inflammation in activated macrophages.
    Authors: Scatizzi JC, Mavers M, Hutcheson J, Young B, Shi B, Pope RM, Ruderman EM, Samways DS, Corbett JA, Egan TM, Perlman H
    Eur. J. Immunol., 2009;39(3):820-5.
    Species: Human
    Sample Types: Cell Culture Supernates
  95. Divergent effects of hypoxia on dendritic cell functions.
    Authors: Mancino A, Schioppa T, Larghi P, Pasqualini F, Nebuloni M, Chen IH, Sozzani S, Austyn JM, Mantovani A, Sica A
    Blood, 2008;112(9):3723-34.
    Species: Human
    Sample Types: Cell Culture Supernates
  96. Impact of tobacco-smoke on key signaling pathways in the innate immune response in lung macrophages.
    Authors: Birrell MA, Wong S, Catley MC, Belvisi MG
    J. Cell. Physiol., 2008;214(1):27-37.
    Species: Human
    Sample Types: Cell Culture Supernates
  97. Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.
    Authors: Wu T, Xie C, Wang HW, Zhou XJ, Schwartz N, Calixto S, Mackay M, Aranow C, Putterman C, Mohan C
    J. Immunol., 2007;179(10):7166-75.
    Species: Human
    Sample Types: Urine
  98. Novel role for the liver X nuclear receptor in the suppression of lung inflammatory responses.
    Authors: Birrell MA, Catley MC, Hardaker E, Wong S, Willson TM, McCluskie K, Leonard T, Farrow SN, Collins JL, Haj-Yahia S, Belvisi MG
    J. Biol. Chem., 2007;282(44):31882-90.
    Species: Human
    Sample Types: Cell Culture Supernates
  99. Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling.
    Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M
    J. Clin. Invest., 2007;117(12):3664-3672.
    Species: Human
    Sample Types: Cell Culture Supernates
  100. Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
    Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
    Blood, 2007;110(13):4319-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  101. Early cytokine responses after percutaneous magnetic resonance imaging guided laser thermoablation of malignant liver tumors.
    Authors: Kallio R, Sequeiros R, Surcel HM, Ohtonen P, Kiviniemi H, Syrjala H
    Cytokine, 2007;34(5):278-83.
    Species: Human
    Sample Types: Serum
  102. Sepsis and pathophysiology of anthrax in a nonhuman primate model.
    Authors: Stearns-Kurosawa DJ, Lupu F, Taylor FB, Kinasewitz G, Kurosawa S
    Am. J. Pathol., 2006;169(2):433-44.
    Species: Primate - Papio cynocephalus (Yellow Baboon)
    Sample Types: Plasma
  103. B and T lymphocytes are the primary sources of RANKL in the bone resorptive lesion of periodontal disease.
    Authors: Kawai T, Matsuyama T, Hosokawa Y, Makihira S, Seki M, Karimbux NY, Goncalves RB, Valverde P, Dibart S, Li YP, Miranda LA, Ernst CW, Izumi Y, Taubman MA
    Am. J. Pathol., 2006;169(3):987-98.
    Species: Human
    Sample Types: Tissue Homogenates
  104. Increased ENA-78 in the follicular fluid of patients with endometriosis.
    Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
    Acta Obstet Gynecol Scand, 2006;85(3):336-42.
    Species: Human
    Sample Types: Follicular Fluid
  105. Transmission of Mycobacterium tuberculosis undetected by tuberculin skin testing.
    Authors: Anderson ST, Williams AJ, Brown JR, Newton SM, Simsova M, Nicol MP, Sebo P, Levin M, Wilkinson RJ, Wilkinson KA
    Am. J. Respir. Crit. Care Med., 2006;173(9):1038-42.
    Species: Human
    Sample Types: Cell Culture Supernates
  106. Role of matrix metalloproteinases in the inflammatory response in human airway cell-based assays and in rodent models of airway disease.
    Authors: Birrell MA, Wong S, Dekkak A, De Alba J, Haj-Yahia S, Belvisi MG
    J. Pharmacol. Exp. Ther., 2006;318(2):741-50.
    Species: Human
    Sample Types: Cell Culture Supernates
  107. Synergistic stimulation of human monocytes and dendritic cells by Toll-like receptor 4 and NOD1- and NOD2-activating agonists.
    Authors: Fritz JH, Girardin SE, Fitting C, Werts C, Mengin-Lecreulx D, Caroff M, Cavaillon JM, Philpott DJ, Adib-Conquy M
    Eur. J. Immunol., 2005;35(8):2459-70.
    Species: Human
    Sample Types: Cell Culture Supernates
  108. Adherence influences monocyte responsiveness to interleukin-10.
    Authors: Petit-Bertron AF, Fitting C, Cavaillon JM, Adib-Conquy M
    J. Leukoc. Biol., 2003;73(1):145-54.
    Species: Human
    Sample Types: Cell Culture Supernates
  109. Differential effect of serotonin on cytokine production in lipopolysaccharide-stimulated human peripheral blood mononuclear cells: involvement of 5-hydroxytryptamine2A receptors.
    Authors: Cloez-Tayarani I, Petit-Bertron AF, Venters HD, Cavaillon JM
    Int. Immunol., 2003;15(2):233-40.
    Species: Human
    Sample Types: Cell Culture Supernates
  110. Comparison of inflammatory elements in nasal lavage and induced sputum following occupational exposure to moldy-building microbes.
    Authors: Purokivi M, Hirvonen MR, Roponen M, Randell J, Vahteristo M, Tukiainen H
    Inhal Toxicol, 2002;14(6):653-62.
    Species: Human
    Sample Types: Sputum
  111. IL-1, IL-6 and TNF-alpha concentrations in the peritoneal fluid of women with pelvic adhesions.
    Authors: Cheong YC, Shelton JB, Laird SM, Richmond M, Kudesia G, Li TC, Ledger WL
    Hum. Reprod., 2002;17(1):69-75.
    Species: Human
    Sample Types: Peritoneal Fluid
  112. The correlation of adhesions and peritoneal fluid cytokine concentrations: a pilot study.
    Authors: Cheong YC, Laird SM, Shelton JB, Ledger WL, Li TC, Cooke ID
    Hum. Reprod., 2002;17(4):1039-45.
    Species: Human
    Sample Types: Peritoneal Fluid
  113. Inflammatory mediators in nasal lavage, induced sputum and serum of employees with rheumatic and respiratory disorders.
    Authors: Roponen M, Kiviranta J, Seuri M, Tukiainen H, Myllykangas-Luosujarvi R, Hirvonen MR
    Eur. Respir. J., 2001;18(3):542-8.
    Species: Human
    Sample Types: Serum

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Human IL-1 beta/IL-1F2 DuoSet ELISA
By Anonymous on 11/26/2018
Sample Tested: Plasma

Human IL-1 beta/IL-1F2 DuoSet ELISA
By Anonymous on 10/07/2018
Sample Tested: EDTA Plasma

Human IL-1 beta/IL-1F2 DuoSet ELISA
By Anonymous on 07/03/2018
Sample Tested: THP-1 human acute monocytic leukemia cell line

Human IL-1 beta/IL-1F2 DuoSet ELISA
By Anonymous on 04/12/2018
Sample Tested: Cell culture supernatant

Human IL-1 beta/IL-1F2 DuoSet ELISA
By Anonymous on 03/30/2018
Sample Tested: Serum

Human IL-1 beta/IL-1F2 DuoSet ELISA
By Anonymous on 02/27/2018
Sample Tested: 7TD1 mouse hybridoma cell line,Human Whole Blood

Human IL-1 beta/IL-1F2 DuoSet ELISA
By Anonymous on 02/06/2018
Sample Tested: Bone marrow-derived dendritic cells

Human IL-1 beta/IL-1F2 DuoSet ELISA
By Anonymous on 01/25/2018
Sample Tested: THP-1 human acute monocytic leukemia cell line

Human IL-1 beta/IL-1F2 DuoSet ELISA
By Anonymous on 10/17/2017
Sample Tested: U937 human histiocytic lymphoma cell line

Human IL-1 beta/IL-1F2 DuoSet ELISA
By Anonymous on 09/08/2017
Sample Tested: Activated mouse CD8 T cell

Human IL-1 beta/IL-1F2 DuoSet ELISA
By Anonymous on 04/03/2017
Sample Tested: Human Serum

Human IL-1 beta/IL-1F2 DuoSet ELISA, 15 Plate
By Anonymous on 04/04/2016
Sample Tested: Various primary cells,THP-1 human acute monocytic leukemia cell line