Human IL-10 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY217B
DY217B-05
Ancillary Products Available
Human IL-10 ELISA Standard Curve
2 Images
Product Details
Procedure
Citations (102)
FAQs
Supplemental Products
Reviews (9)

Human IL-10 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IL-10. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Data Examples

Human IL-10 ELISA Standard Curve

Human IL-10 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-10

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 alpha -helical cytokine family that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1-3). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (4, 5). Mature human IL-10 shares 86% amino acid sequence identity with equine IL-10 and 72% - 80% with bovine, canine, feline, guinea pig, mouse, ovine, porcine, and rat IL-10. It contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently-associated homodimer (4, 6, 7). 

IL-10 mediates its biological activities through a heteromeric receptor complex composed of the type II cytokine receptor subunits IL-10 R alpha and IL-10 R beta. IL-10 R alpha is a 110 kDa transmembrane glycoprotein that is expressed on lymphocytes, NK cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells (8-13), while the 75 kDa transmembrane IL-10 R beta is widely expressed (14, 15). The IL-10 dimer binds to two IL-10 R alpha chains, triggering recruitment of two IL-10 R beta chains (14, 15). IL-10 R beta does not bind IL-10 directly but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (16-18). 
The involvement of IL-10 in immunoregulation includes both suppressive and stimulatory effects. It functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells (19-21) and by promoting the development of M2 macrophages (21). Its expression by immunosuppressive regulatory T cells (Treg) and regulatory B cells is important for Treg proliferation (19). Within a tumor microrenvironment, however, IL-10 inhibits the expansion of Treg as well as myeloid-derived suppressor cells (22, 23). IL-10 induces the intratumoral accumulation and activation of CD8+ T cells (24, 25). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation (19) and promoting muscle regeneration after injury (21), but it also contributes to the persistence of viral infections (26). The levels of IL-10 are elevated in Sjogren's syndrome (saliva), primary CNS lymphoma (cerebrospinal fluid), and ovarian cancer (serum and ascites) (27-29). Its levels are decreased in the serum in patients with recurrent heart attacks or during preeclampsia and also in the seminal fluid of infertile men (30-32).

Long Name:
Interleukin 10
Entrez Gene IDs:
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Alternate Names:
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

 

Citations for Human IL-10 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

102 Citations: Showing 1 - 10
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  1. Narciclasine, an isocarbostyril alkaloid, has preferential activity against primary effusion lymphoma
    Authors: R Gopalakris, H Matta, S Choi, PM Chaudhary
    Sci Rep, 2020;10(1):5712.
    Species: Mouse
    Sample Types: Plasma
  2. Gaps in Study Design for Immune Parameter Research for Latent Tuberculosis Infection: A Systematic Review
    Authors: M Herrera, C Vera, Y Keynan, ZV Rueda
    J Immunol Res, 2020;2020(0):8074183.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. β-Glucan-Induced Trained Immunity Protects against Leishmania braziliensis Infection: a Crucial Role for IL-32
    Authors: JC Dos Santos, AM Barroso de, MV Teodoro Si, B Cirovic, LCJ de Bree, MSMA Damen, SJCFM Moorlag, RS Gomes, MM Helsen, M Oosting, ST Keating, A Schlitzer, MG Netea, F Ribeiro-Di, LAB Joosten
    Cell Rep, 2019;28(10):2659-2672.e6.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Effects of continuous moderate exercise with partial blood flow restriction during hemodialysis: A protocol for a randomized clinical trial
    Authors: RK Cardoso, AM Araujo, RB Orcy, M Bohlke, JP Oses, FB Del Vecchi, FC Barcellos, MC Gonzalez, AJ Rombaldi
    MethodsX, 2019;6(0):190-198.
    Species: Human
    Sample Types: Serum
  5. D-2-Hydroxyglutarate and L-2-Hydroxyglutarate Inhibit IL-12 Secretion by Human Monocyte-Derived Dendritic Cells
    Authors: I Ugele, ZE Cárdenas-C, K Hammon, M Wehrstein, C Bruss, K Peter, K Singer, E Gottfried, J Boesch, P Oefner, K Dettmer, K Renner, M Kreutz
    Int J Mol Sci, 2019;20(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Large-scale secretome analyses unveil the superior immunosuppressive phenotype of umbilical cord stromal cells as compared to other adult mesenchymal stromal cells
    Authors: A Islam, I Urbarova, JA Bruun, I Martinez-Z
    Eur Cell Mater, 2019;37(0):153-174.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Lower levels of leptin are associated with severity parameters in visceral leishmaniasis patients
    Authors: AMDC Fievez, ML Silva-Frei, AQ Sousa, JR Santos-Oli, AM Da-Cruz
    PLoS ONE, 2019;14(3):e0214413.
    Species: Human
    Sample Types: Serum
  8. Effect of Propofol on the Production of Inflammatory Cytokines by Human Polarized Macrophages
    Authors: T Kochiyama, X Li, H Nakayama, M Kage, Y Yamane, K Takamori, K Iwabuchi, E Inada
    Mediators Inflamm., 2019;2019(0):1919538.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Role of vimentin in modulating immune cell apoptosis and inflammatory responses in sepsis
    Authors: L Su, P Pan, P Yan, Y Long, X Zhou, X Wang, R Zhou, B Wen, L Xie, D Liu
    Sci Rep, 2019;9(1):5747.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Newly defined ABCB5+ dermal mesenchymal stem cells promote healing of chronic iron overload wounds via secretion of interleukin-1 receptor antagonist
    Authors: S Vander Bek, JC de Vries, B Meier-Schi, P Meyer, D Jiang, A Sindrilaru, FF Ferreira, A Hainzl, S Schatz, J Muschhamme, NJ Scheurmann, P Kampilafko, AM Seitz, L Dürselen, A Ignatius, MA Kluth, C Ganss, M Wlaschek, K Singh, P Maity, NY Frank, MH Frank, K Scharffett
    Stem Cells, 2019;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Crosstalk between cancer cells and tumor associated macrophages is required for mesenchymal circulating tumor cell-mediated colorectal cancer metastasis
    Authors: C Wei, C Yang, S Wang, D Shi, C Zhang, X Lin, Q Liu, R Dou, B Xiong
    Mol. Cancer, 2019;18(1):64.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Human immune cells infiltrate the spinal cord and impair recovery after spinal cord injury in humanized mice
    Authors: RS Carpenter, RR Jiang, FH Brennan, JCE Hall, MK Gottipati, S Niewiesk, PG Popovich
    Sci Rep, 2019;9(1):19105.
    Species: Transgenic Mouse
    Sample Types: Cell Culture Supernates
  13. Comparative Investigation of Frankincense Nutraceuticals: Correlation of Boswellic and Lupeolic Acid Contents with Cytokine Release Inhibition and Toxicity against Triple-Negative Breast Cancer Cells
    Authors: M Schmiech, SJ Lang, J Ulrich, K Werner, LJ Rashan, T Syrovets, T Simmet
    Nutrients, 2019;11(10):.
    Species: Human
    Sample Types: Plasma
  14. Echinochrome A Reduces Colitis in Mice and Induces In Vitro Generation of Regulatory Immune Cells
    Authors: SJ Oh, Y Seo, JS Ahn, YY Shin, JW Yang, HK Kim, J Han, NP Mishchenko, SA Fedoreyev, VA Stonik, HS Kim
    Mar Drugs, 2019;17(11):.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Replication of Genome-Wide Association Analysis Identifies New Susceptibility Loci at Long Noncoding RNA Regions for Vogt-Koyanagi-Harada Disease
    Authors: J Qi, L Du, J Deng, Y Qin, G Su, S Hou, M Lv, Q Zhang, A Kijlstra, P Yang
    Invest. Ophthalmol. Vis. Sci., 2019;60(14):4820-4829.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Insufficient IL-10 Production as a Mechanism Underlying the Pathogenesis of Systemic Juvenile Idiopathic Arthritis
    Authors: M Imbrechts, A Avau, J Vandenhaut, B Malengier-, K Put, T Mitera, N Berghmans, O Burton, S Junius, A Liston, L de Somer, C Wouters, P Matthys
    J. Immunol., 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Proteome Analysis Reveals the Conidial Surface Protein CcpA Essential for Virulence of the Pathogenic Fungus Aspergillus fumigatus
    Authors: V Voltersen, MG Blango, S Herrmann, F Schmidt, T Heinekamp, M Strassburg, T Krüger, P Bacher, J Lother, E Weiss, K Hünniger, H Liu, P Hortschans, A Scheffold, J Löffler, S Krappmann, S Nietzsche, O Kurzai, H Einsele, O Kniemeyer, SG Filler, U Reichard, AA Brakhage
    MBio, 2018;9(5):.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Fecal Galectin-3: A New Promising Biomarker for Severity and Progression of Colorectal Carcinoma
    Authors: M Jovanovic, N Gajovic, N Zdravkovic, M Jovanovic, M Jurisevic, D Vojvodic, V Maric, A Arsenijevi, I Jovanovic
    Mediators Inflamm., 2018;2018(0):8031328.
    Species: Human
    Sample Types: Feces
  19. Annexin A1, FPR2/ALX, and inflammatory cytokine expression in peritoneal endometriosis
    Authors: LK Volpato, VV Horewicz, F Bobinski, DF Martins, AP Piovezan
    J. Reprod. Immunol., 2018;129(0):30-35.
    Species: Human
    Sample Types: Peritoneal Fluid
  20. Decreased allergy incidence in children supplemented with E. coli O83:K24:H31 and its possible modes of action
    Authors: J Hrdý, K Vlasáková, V ?erný, L Súkeníková, O Novotná, P Petrásková, K Boráková, R Lodinová-Ž, L Kolá?ová, L Prokešová
    Eur. J. Immunol., 2018;0(0):.
    Species: Human
    Sample Types: Plasma
  21. Human Mesenchymal Stem Cell Secretome from Bone Marrow or Adipose-Derived Tissue Sources for Treatment of Hypoxia-Induced Pulmonary Epithelial Injury
    Authors: N Shologu, M Scully, JG Laffey, D O'Toole
    Int J Mol Sci, 2018;19(10):.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Diminished circulating plasma and elevated lymph node culture supernatant levels of IL-10 family cytokines in tuberculous lymphadenitis
    Authors: GR Kathamuthu, NP Kumar, K Moideen, D Baskaran, S Hissar, BM Shrinivasa, R Sridhar, S Babu
    Cytokine, 2018;0(0):.
    Species: Human
    Sample Types: Plasma
  23. PDGF enhances the protective effect of adipose stem cell-derived extracellular vesicles in a model of acute hindlimb ischemia
    Authors: T Lopatina, E Favaro, C Grange, M Cedrino, A Ranghino, S Occhipinti, S Fallo, F Buffolo, DA Gaykalova, MM Zanone, R Romagnoli, G Camussi
    Sci Rep, 2018;8(1):17458.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Combining Calcium Phosphates with Polysaccharides: A Bone-Inspired Material Modulating Monocyte/Macrophage Early Inflammatory Response
    Authors: H Rammal, C Bour, M Dubus, L Entz, L Aubert, SC Gangloff, S Audonnet, NB Bercu, F Boulmedais, C Mauprivez, H Kerdjoudj
    Int J Mol Sci, 2018;19(11):.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Salmonella Activation of STAT3 Signaling by SarA Effector Promotes Intracellular Replication and Production of IL-10
    Authors: SL Jaslow, KD Gibbs, WF Fricke, L Wang, KJ Pittman, MK Mammel, JT Thaden, VG Fowler, GE Hammer, JR Elfenbein, DC Ko
    Cell Rep, 2018;23(12):3525-3536.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Plasminogen Activator Inhibitor-1 Promotes the Recruitment and Polarization of Macrophages in Cancer
    Authors: MH Kubala, V Punj, VR Placencio-, H Fang, GE Fernandez, R Sposto, YA DeClerck
    Cell Rep, 2018;25(8):2177-2191.e7.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Plasmodium falciparum Treated with Artemisinin-based Combined Therapy Exhibits Enhanced Mutation, Heightened Cortisol and TNF-? Induction
    Authors: AO Idowu, S Bhattachar, S Gradus, W Oyibo, Z George, C Black, J Igietseme, AA Azenabor
    Int J Med Sci, 2018;15(13):1449-1457.
    Species: Human
    Sample Types: Plasma
  28. Association of Long Noncoding RNAs Polymorphisms With Ankylosing Spondylitis, Vogt-Koyanagi-Harada Disease, and Behcet's Disease
    Authors: Y Yue, J Zhang, L Yang, S Liu, J Qi, Q Cao, C Zhou, Y Wang, A Kijlstra, P Yang, S Hou
    Invest. Ophthalmol. Vis. Sci., 2018;59(2):1158-1166.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Analysis of the association between Fc receptor family gene polymorphisms and ocular Beh�et's disease in Han Chinese
    Authors: D Zhang, J Qin, L Li, G Su, G Huang, Q Cao, A Kijlstra, P Yang
    Sci Rep, 2018;8(1):4850.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Relationship of systemic IL-10 levels with proinflammatory cytokine responsiveness and lung function in agriculture workers.
    Authors: Tricia D LeVan, Debra J Romberger, Mohammad Siahpush, Brandon L Grimm, Athena K Ramos, Patrik L Johansson, Tzeyu L Michaud, Art J Heires, Todd A Wyatt, Jill A Poole
    Respiratory Research, 2018;0(0):1465-993X.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Helicobacter pylori-derived heat shock protein 60 increases the induction of regulatory T-cells associated with persistent infection
    Authors: WT Hsu, SY Ho, TY Jian, HN Huang, YL Lin, CH Chen, TH Lin, MS Wu, CJ Wu, YL Chan, KW Liao
    Microb. Pathog., 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Dexamethasone induced inhibition of Dectin-1 activation of antigen presenting cells is mediated via STAT-3 and NF-?B signaling pathways.
    Authors: Philipp Kotthoff, Annkristin Heine, Stefanie Andrea Erika Held, Peter Brossart
    Scientific Reports, 2017;0(0):2045-2322.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Collagen-Based Medical Device as a Stem Cell Carrier for Regenerative Medicine
    Authors: L Aubert, M Dubus, H Rammal, C Bour, C Mongaret, C Boulagnon-, R Garnotel, C Schneider, R Rahouadj, C Laurent, SC Gangloff, F Velard, C Mauprivez, H Kerdjoudj
    Int J Mol Sci, 2017;18(10):.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. The role of indoleamine 2,3-dioxygenase-aryl hydrocarbon receptor pathway in the TLR4-induced tolerogenic phenotype in human DCs
    Authors: F Salazar, D Awuah, OH Negm, F Shakib, AM Ghaemmagha
    Sci Rep, 2017;7(0):43337.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Interleukin-1 primes human mesenchymal stem cells towards an anti-inflammatory and pro-trophic phenotype in vitro
    Authors: E Redondo-Ca, C Cunningham, J Miller, L Martuscell, S Aoulad-Ali, NJ Rothwell, CM Kielty, SM Allan, E Pinteaux
    Stem Cell Res Ther, 2017;8(1):79.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. Neutrophil Extracellular Traps Reprogram IL-4/GM-CSF-Induced Monocyte Differentiation to Anti-inflammatory Macrophages
    Authors: AB Guimarães-, NC Rochael, F Oliveira, J Echevarria, EM Saraiva
    Front Immunol, 2017;8(0):523.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. Periodontitis and type 2 diabetes among women with previous gestational diabetes: epidemiological and immunological aspects in a follow-up of three years
    Authors: RP Esteves Li, LO Cota, TA Silva, SC Cortelli, JR Cortelli, FO Costa
    J Appl Oral Sci, 2017;25(2):130-139.
    Species: Human
    Sample Types: Saliva
  38. Receptor-Type Protein-Tyrosine Phosphatase ? and Colony Stimulating Factor-1 Receptor in the Intestine: Cellular Expression and Cytokine- and Chemokine Responses by Interleukin-34 and Colony Stimulating Factor-1
    PLoS ONE, 2016;11(11):e0167324.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. Reduced systemic and mycobacterial antigen-stimulated concentrations of IL-1? and IL-18 in tuberculous lymphadenitis
    Cytokine, 2016;90(0):66-72.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Genetic polymorphisms of cell adhesion molecules in Behcet's disease in a Chinese Han population
    Authors: M Zheng, L Zhang, H Yu, J Hu, Q Cao, G Huang, Y Huang, G Yuan, A Kijlstra, P Yang
    Sci Rep, 2016;6(0):24974.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Acanthamoeba castellanii (Genotype T4) Stimulates the Production of Interleukin-10 as well as Pro-inflammatory Cytokines in THP-1 Cells, Human Peripheral Blood Mononuclear Cells and Human Monocyte-Derived Macrophages
    Infect Immun, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. Expression of Tim-1 in primary CNS lymphoma
    Cancer Med, 2016;5(11):3235-3245.
    Species: Human
    Sample Types: Cell Culture Supernates
  43. Soluble alpha-enolase activates monocytes by CD14-dependent TLR4 signalling pathway and exhibits a dual function
    Authors: C Guillou, M Fréret, E Fondard, C Derambure, G Avenel, ML Golinski, M Verdet, O Boyer, F Caillot, P Musette, T Lequerré, O Vittecoq
    Sci Rep, 2016;6(0):23796.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Serological signatures of clinical cure following successful treatment with sodium stibogluconate in Ethiopian visceral leishmaniasis
    Cytokine, 2016;91(0):6-9.
    Species: Human
    Sample Types: Serum
  45. The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
    Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F
    Leukemia, 2016;30(1):163-72.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. Analysis of receptor tyrosine kinase genetics identifies two novel risk loci in GAS6 and PROS1 in Beh�et's disease
    Sci Rep, 2016;6(0):26662.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. The effects of cytokines on spontaneous hepatitis B surface antigen seroconversion in chronic hepatitis B virus infection.
    Authors: Wu J, Hsu H, Chiu Y, Chen H, Ni Y, Chang M
    J Immunol, 2015;194(2):690-6.
    Species: Human
    Sample Types: Serum
  48. In vitro progesterone modulation on bacterial endotoxin-induced production of IL-1beta, TNFalpha, IL-6, IL-8, IL-10, MIP-1alpha, and MMP-9 in pre-labor human term placenta.
    Authors: Garcia-Ruiz G, Flores-Espinosa P, Preciado-Martinez E, Bermejo-Martinez L, Espejel-Nunez A, Estrada-Gutierrez G, Maida-Claros R, Flores-Pliego A, Zaga-Clavellina V
    Reprod Biol Endocrinol, 2015;13(0):115.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. Characterization of host and microbial determinants in individuals with latent tuberculosis infection using a human granuloma model.
    Authors: Guirado E, Mbawuike U, Keiser T, Arcos J, Azad A, Wang S, Schlesinger L
    MBio, 2015;6(1):e02537-14.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. Sourcing of an alternative pericyte-like cell type from peripheral blood in clinically relevant numbers for therapeutic angiogenic applications.
    Authors: Blocki A, Wang Y, Koch M, Goralczyk A, Beyer S, Agarwal N, Lee M, Moonshi S, Dewavrin J, Peh P, Schwarz H, Bhakoo K, Raghunath M
    Mol Ther, 2015;23(3):510-22.
    Species: Human
    Sample Types: Cell Culture Supernates
  51. LPS-Stimulated Whole Blood Cytokine Production Is Not Related to Disease Behavior in Patients with Quiescent Crohn's Disease.
    Authors: Broekman M, Roelofs H, Hoentjen F, Wiegertjes R, Stoel N, Joosten L, de Jong D, Wanten G
    PLoS ONE, 2015;10(7):e0133932.
    Species: Human
    Sample Types: Plasma
  52. Exposure to diesel exhaust particle extracts (DEPe) impairs some polarization markers and functions of human macrophages through activation of AhR and Nrf2.
    Authors: Jaguin M, Fardel O, Lecureur V
    PLoS ONE, 2015;10(2):e0116560.
    Species: Human
    Sample Types: Cell Culture Supernates
  53. Ligation of TLR7 on CD19(+) CD1d(hi) B cells suppresses allergic lung inflammation via regulatory T cells.
    Authors: Khan A, Amu S, Saunders S, Hams E, Blackshields G, Leonard M, Weaver C, Sparwasser T, Sheils O, Fallon P
    Eur J Immunol, 2015;45(6):1842-54.
    Species: Human
    Sample Types: Cell Culture Supernates
  54. Macrophages and dendritic cells as actors in the immune reaction of classical Hodgkin lymphoma.
    Authors: Tudor C, Bruns H, Daniel C, Distel L, Hartmann A, Gerbitz A, Buettner M
    PLoS ONE, 2015;9(12):e114345.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. IFN- alpha blocks IL-17 production by peripheral blood mononuclear cells in patients with chronic active hepatitis B Infection.
    Authors: Cui F, Meng J, Luo P, Chen P
    BMC Infect Dis, 2014;14(0):55.
    Species: Human
    Sample Types: Cell Culture Supernates
  56. Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study.
    Authors: Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K
    BMC Infect Dis, 2014;14(0):35.
    Species: Human
    Sample Types: Serum
  57. IFNgamma/IL-10 co-producing cells dominate the CD4 response to malaria in highly exposed children.
    Authors: Jagannathan P, Eccles-James I, Bowen K, Nankya F, Auma A, Wamala S, Ebusu C, Muhindo M, Arinaitwe E, Briggs J, Greenhouse B, Tappero J, Kamya M, Dorsey G, Feeney M
    PLoS Pathog, 2014;10(1):e1003864.
    Species: Human
    Sample Types: Plasma
  58. TNF-alpha blockade induces IL-10 expression in human CD4+ T cells.
    Authors: Evans H, Roostalu U, Walter G, Gullick N, Frederiksen K, Roberts C, Sumner J, Baeten D, Gerwien J, Cope A, Geissmann F, Kirkham B, Taams L
    Nat Commun, 2014;5(0):3199.
    Species: Human
    Sample Types: Cell Culture Supernates
  59. A functional variant of PTPN22 confers risk for Vogt-Koyanagi-Harada syndrome but not for ankylosing spondylitis.
    Authors: Zhang Q, Qi J, Hou S, DU L, Yu H, Cao Q, Zhou Y, Liao D, Kijlstra A, Yang P
    PLoS ONE, 2014;9(5):e96943.
    Species: Human
    Sample Types: Cell Culture Supernates
  60. CD137 ligand signalling induces differentiation of primary acute myeloid leukaemia cells.
    Authors: Cheng K, Wong S, Linn Y, Ho L, Chng W, Schwarz H
    Br J Haematol, 2014;165(1):134-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  61. cmvIL-10 stimulates the invasive potential of MDA-MB-231 breast cancer cells.
    Authors: Valle Oseguera, Cendy A, Spencer, Juliet V
    PLoS ONE, 2014;9(2):e88708.
    Species: Human
    Sample Types: Cell Culture Supernates
  62. Human CD4+ T cell responses to the dog major allergen Can f 1 and its human homologue tear lipocalin resemble each other.
    Authors: Liukko A, Kinnunen T, Rytkonen-Nissinen M, Kailaanmaki A, Randell J, Maillere B, Virtanen T
    PLoS ONE, 2014;9(5):e98461.
    Species: Human
    Sample Types: Cell Culture Supernates
  63. Tumour microenvironment of both early- and late-stage colorectal cancer is equally immunosuppressive.
    Authors: O'Toole A, Michielsen A, Nolan B, Tosetto M, Sheahan K, Mulcahy H, Winter D, Hyland J, O'Connell P, Fennelly D, O'Donoghue D, O'Sullivan J, Doherty G, Ryan E
    Br J Cancer, 2014;111(5):927-32.
    Species: Human
    Sample Types: Cell Culture Supernates
  64. Immunoepidemiological profiling of onchocerciasis patients reveals associations with microfilaria loads and ivermectin intake on both individual and community levels.
    Authors: Arndts, Kathrin, Specht, Sabine, Debrah, Alexande, Tamarozzi, Francesc, Klarmann Schulz, Ute, Mand, Sabine, Batsa, Linda, Kwarteng, Alexande, Taylor, Mark, Adjei, Ohene, Martin, Coralie, Layland, Laura E, Hoerauf, Achim
    PLoS Negl Trop Dis, 2014;8(2):e2679.
    Species: Human
    Sample Types: Cell Culture Supernates
  65. Autocrine IL-10 promotes human B-cell differentiation into IgM- or IgG-secreting plasmablasts.
    Authors: Heine G, Drozdenko G, Grun J, Chang H, Radbruch A, Worm M
    Eur J Immunol, 2014;44(6):1615-21.
    Species: Human
    Sample Types: Cell Culture Supernates
  66. Independent and interdependent immunoregulatory effects of IL-27, IFN-beta, and IL-10 in the suppression of human Th17 cells and murine experimental autoimmune encephalomyelitis.
    Authors: Fitzgerald D, Fonseca-Kelly Z, Cullimore M, Safabakhsh P, Saris C, Zhang G, Rostami A
    J Immunol, 2013;190(7):3225-34.
    Species: Human
    Sample Types: Cell Culture Supernates
  67. Selective activation of human dendritic cells by OM-85 through a NF-kB and MAPK dependent pathway.
    Authors: Parola C, Salogni L, Vaira X, Scutera S, Somma P, Salvi V, Musso T, Tabbia G, Bardessono M, Pasquali C, Mantovani A, Sozzani S, Bosisio D
    PLoS ONE, 2013;8(12):e82867.
    Species: Human
    Sample Types: Cell Culture Supernates
  68. Association of TLR2 gene polymorphisms with ocular Behcet's disease in a Chinese Han population.
    Authors: Fang J, Hu R, Hou S, Ye Z, Xiang Q, Qi J, Zhou Y, Kijlstra A, Yang P
    Invest Ophthalmol Vis Sci, 2013;54(13):8384-92.
    Species: Human
    Sample Types: Cell Culture Supernates
  69. Leishmania braziliensis-reactive T cells are down-regulated in long-term cured cutaneous Leishmaniasis, but the renewal capacity of T effector memory compartments is preserved.
    Authors: Pereira-Carvalho R, Mendes-Aguiar C, Oliveira-Neto M, Covas C, Bertho A, Da-Cruz A, Gomes-Silva A
    PLoS ONE, 2013;8(11):e81529.
    Species: Human
    Sample Types: Cell Culture Supernates
  70. Quiescent innate response to infective filariae by human Langerhans cells suggests a strategy of immune evasion.
    Authors: Boyd A, Bennuru S, Wang Y, Sanprasert V, Law M, Chaussabel D, Nutman T, Semnani R
    Infect Immun, 2013;81(5):1420-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  71. Impact of vitamin D supplementation on markers of inflammation in adults with cystic fibrosis hospitalized for a pulmonary exacerbation.
    Eur J Clin Nutr, 2012;66(9):1072-4.
    Species: Human
    Sample Types: Serum
  72. Characterization and function of the human macrophage dopaminergic system: implications for CNS disease and drug abuse.
    J Neuroinflammation, 2012;9(0):203.
    Species: Human
    Sample Types: Cell Culture Supernates
  73. Elevated adaptive immune responses are associated with latent infections of Wuchereria bancrofti.
    Authors: Arndts K, Deininger S, Specht S, Klarmann U, Mand S, Adjobimey T, Debrah AY, Batsa L, Kwarteng A, Epp C, Taylor M, Adjei O, Layland LE, Hoerauf A
    PLoS Negl Trop Dis, 2012;6(4):e1611.
    Species: Human
    Sample Types: Cell Culture Supernates
  74. Regulation of progranulin expression in human microglia and proteolysis of progranulin by matrix metalloproteinase-12 (MMP-12).
    Authors: Suh HS, Choi N, Tarassishin L, Lee SC
    PLoS ONE, 2012;7(4):e35115.
    Species: Human
    Sample Types: Cell Culture Supernates
  75. Urinary neutrophil gelatinase-associated lipocalin is a potential biomarker for renal damage in patients with systemic lupus erythematosus.
    Authors: Yang CC, Hsieh SC, Li KJ, Wu CH, Lu MC, Tsai CY, Yu CL
    J. Biomed. Biotechnol., 2012;2012(0):759313.
    Species: Human
    Sample Types: Urine
  76. Possible involvement of IL-21 and IL-10 on salivary IgA levels in chronic periodontitis subjects.
    Authors: Napimoga MH, Nunes LH, Maciel AA, Demasi AP, Benatti BB, Santos VR, Bastos MF, de Miranda TS, Duarte PM
    Scand. J. Immunol., 2011;74(6):596-602.
    Species: Human
    Sample Types: Tissue Homogenates
  77. IFN-alpha blocks IL-17 production by peripheral blood mononuclear cells in Behcet's disease.
    Authors: Liu X, Yang P, Wang C, Li F, Kijlstra A
    Rheumatology (Oxford), 2011;50(2):293-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  78. Human uterine epithelial cell secretions regulate dendritic cell differentiation and responses to TLR ligands.
    Authors: Ochiel DO, Ghosh M, Fahey JV
    J. Leukoc. Biol., 2010;88(3):435-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  79. Immunoregulation of autocrine prolactin: suppressing the expression of costimulatory molecules and cytokines in T lymphocytes by prolactin receptor knockdown.
    Authors: Xu D, Lin L, Lin X
    Cell. Immunol., 2010;263(1):71-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  80. PEGylation of interleukin-10 for the mitigation of enhanced pain states.
    Authors: Soderquist RG, Milligan ED, Harrison JA, Chavez RA, Johnson KW, Watkins LR, Mahoney MJ
    J Biomed Mater Res A, 2010;93(3):1169-79.
    Species: Human
    Sample Types: CSF
  81. IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
    Authors: Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Immunol., 2010;184(9):4880-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  82. Anemia in Hodgkin's lymphoma: the role of interleukin-6 and hepcidin.
    Authors: Hohaus S, Massini G, Giachelia M, Vannata B, Bozzoli V, Cuccaro A, D'Alo' F, Larocca LM, Raymakers RA, Swinkels DW, Voso MT, Leone G
    J. Clin. Oncol., 2010;28(15):2538-43.
    Species: Human
    Sample Types: Plasma
  83. Investigation of functional IL-10 gene polymorphism and IL-10 levels in acute graft-versus-host disease.
    Authors: Resende RG, Correia-Silva Jde F, Arao TC, Silva TA, Abreu MH, Bittencourt H, Gomez RS
    J. Clin. Immunol., 2010;30(3):465-73.
    Species: Human
    Sample Types: Saliva
  84. Reduced ex vivo interleukin-6 production by dietary fish oil is not modified by linoleic acid intake in healthy men.
    Authors: Damsgaard CT, Lauritzen L, Calder PC, Kjaer TR, Frokiaer H
    J. Nutr., 2009;139(7):1410-4.
    Species: Human
    Sample Types: Cell Culture Supernates
  85. Clinical significance of interleukin-10 gene polymorphisms and plasma levels in Hodgkin lymphoma.
    Authors: Hohaus S, Giachelia M, Massini G, Vannata B, Criscuolo M, Martini M, D'Alo' F, Voso MT, Larocca LM, Leone G
    Leuk. Res., 2009;33(10):1352-6.
    Species: Human
    Sample Types: Plasma
  86. Helminth antigens modulate immune responses in cells from multiple sclerosis patients through TLR2-dependent mechanisms.
    Authors: Correale J, Farez M
    J. Immunol., 2009;183(9):5999-6012.
    Species: Human
    Sample Types: Cell Culture Supernates
  87. Reduced response to adiponectin and lower abundance of adiponectin receptor proteins in type 2 diabetic monocytes.
    Authors: Weigert J, Neumeier M, Wanninger J, Wurm S, Kopp A, Schober F, Filarsky M, Schaffler A, Zeitoun M, Aslanidis C, Buechler C
    FEBS Lett., 2008;582(12):1777-82.
    Species: Human
    Sample Types: Cell Culture Supernates
  88. Divergent effects of hypoxia on dendritic cell functions.
    Authors: Mancino A, Schioppa T, Larghi P, Pasqualini F, Nebuloni M, Chen IH, Sozzani S, Austyn JM, Mantovani A, Sica A
    Blood, 2008;112(9):3723-34.
    Species: Human
    Sample Types: Cell Culture Supernates
  89. Adenosine receptors in regulation of dendritic cell differentiation and function.
    Authors: Novitskiy SV, Ryzhov S, Zaynagetdinov R, Goldstein AE, Huang Y, Tikhomirov OY, Blackburn MR, Biaggioni I, Carbone DP, Feoktistov I, Dikov MM
    Blood, 2008;112(5):1822-31.
    Species: Human
    Sample Types: Cell Culture Supernates
  90. Fungal proteases induce Th2 polarization through limited dendritic cell maturation and reduced production of IL-12.
    Authors: Lamhamedi-Cherradi SE, Martin RE, Ito T, Kheradmand F, Corry DB, Liu YJ, Moyle M
    J. Immunol., 2008;180(9):6000-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  91. Helminth infections associated with multiple sclerosis induce regulatory B cells.
    Authors: Correale J, Farez M, Razzitte G
    Ann. Neurol., 2008;64(2):187-99.
    Species: Human
    Sample Types: Cell Culture Supernates
  92. Lactoferrin, a major defense protein of innate immunity, is a novel maturation factor for human dendritic cells.
    Authors: Spadaro M, Caorsi C, Ceruti P, Varadhachary A, Forni G, Pericle F, Giovarelli M
    FASEB J., 2008;22(8):2747-57.
    Species: Human
    Sample Types: Cell Culture Supernates
  93. Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
    Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
    Blood, 2007;110(13):4319-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  94. The differential effect of Eriobotrya japonica hydrophilic leaf extract on cytokines production and modulation.
    Authors: Matalka KZ, Ali D, Khawad AE, Qa'dan F
    Cytokine, 2007;40(3):235-40.
    Species: Human
    Sample Types: Cell Culture Supernates
  95. B and T lymphocytes are the primary sources of RANKL in the bone resorptive lesion of periodontal disease.
    Authors: Kawai T, Matsuyama T, Hosokawa Y, Makihira S, Seki M, Karimbux NY, Goncalves RB, Valverde P, Dibart S, Li YP, Miranda LA, Ernst CW, Izumi Y, Taubman MA
    Am. J. Pathol., 2006;169(3):987-98.
    Species: Human
    Sample Types: Tissue Homogenates
  96. Synergistic stimulation of human monocytes and dendritic cells by Toll-like receptor 4 and NOD1- and NOD2-activating agonists.
    Authors: Fritz JH, Girardin SE, Fitting C, Werts C, Mengin-Lecreulx D, Caroff M, Cavaillon JM, Philpott DJ, Adib-Conquy M
    Eur. J. Immunol., 2005;35(8):2459-70.
    Species: Human
    Sample Types: Cell Culture Supernates
  97. Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt's lymphoma.
    Authors: Pound JD, Batth BK, Johannessen I, Wood K
    J. Immunol., 2005;174(5):3015-23.
    Species: Human
    Sample Types: Cell Culture Supernates
  98. Association of type 2 cytokines with hepatic fibrosis in human Schistosoma mansoni infection.
    Authors: de Jesus AR, Magalhaes A, Miranda DG, Miranda RG, Araujo MI, de Jesus AA, Silva A, Santana LB, Pearce E, Carvalho EM
    Infect. Immun., 2004;72(6):3391-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  99. The Toll-like receptor-2/6 agonist macrophage-activating lipopeptide-2 cooperates with IFN-gamma to reverse the Th2 skew in an in vitro allergy model.
    Authors: Weigt H, Muhlradt PF, Larbig M, Krug N, Braun A
    J. Immunol., 2004;172(10):6080-6.
    Species: Human
    Sample Types: Cell Culture Supernates
  100. Differential effect of serotonin on cytokine production in lipopolysaccharide-stimulated human peripheral blood mononuclear cells: involvement of 5-hydroxytryptamine2A receptors.
    Authors: Cloez-Tayarani I, Petit-Bertron AF, Venters HD, Cavaillon JM
    Int. Immunol., 2003;15(2):233-40.
    Species: Human
    Sample Types: Cell Culture Supernates
  101. Pleural Effusions from Patients with Mesothelioma Induce Recruitment of Monocytes and Their Differentiation into M2 Macrophages.
    Authors: Chene A, d'Almeida S, Blondy T, Tabiasco J, Deshayes S, Fonteneau J, Cellerin L, Delneste Y, Gregoire M, Blanquart C
    J Thorac Oncol, 0;11(10):1765-73.
    Species: Human
    Sample Types: Cell Culture Supernates
  102. Enhancing CAR T cell persistence through ICOS and 4-1BB costimulation.
    Authors: Guedan S, Posey A, Shaw C, Wing A, Da T, Patel P, McGettigan S, Casado-Medrano V, Kawalekar O, Uribe-Herranz M, Song D, Melenhorst J, Lacey S, Scholler J, Keith B, Young R, June C
    JCI Insight, 0;3(1):.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human IL-10 DuoSet ELISA
By Anonymous on 11/08/2019
Application: Sample Tested: Cell Culture Media

Human IL-10 DuoSet ELISA
By Anonymous on 08/15/2019
Application: Sample Tested: Plasma

Used in both cell culture material (supernatant and lysate) and EDTA plasma. Good intense signal at 450nm corrected for signal at 550nm


Human IL-10 DuoSet ELISA
By Anonymous on 01/11/2019
Application: Sample Tested: HASMC human aortic smooth muscle cells,Human aortic endothelial cells

Human IL-10 DuoSet ELISA
By Anonymous on 01/03/2019
Application: Sample Tested: Cell culture supernatant

Human IL-10 DuoSet ELISA
By Anonymous on 07/24/2018
Application: Sample Tested: Plasma

Human IL-10 DuoSet ELISA
By Anonymous on 05/10/2018
Application: Sample Tested: 2120Ep human embryonal carcinoma cell line

I am processing different type of samples following the same protocols and in for some of the samples the results are not consistent.


Human IL-10 DuoSet ELISA
By Anonymous on 02/22/2018
Application: Sample Tested: THP-1 human acute monocytic leukemia cell line

Human IL-10 DuoSet ELISA
By Anonymous on 08/11/2017
Application: Sample Tested: Serum and Plasma

Human IL-10 DuoSet ELISA
By Anonymous on 11/28/2016
Application: Sample Tested: Human plasma