IC2172C has been discontinued.
Detects human IL-10 in ELISAs. In sandwich immunoassays, less than 5% cross-reactivity with recombinant equine IL-10 is observed and less than 0.5% cross-reactivity with recombinant canine IL-10, recombinant mouse IL-10, recombinant rat IL-10, recombinant porcine IL-10, recombinant cotton rat IL-10, and recombinant feline IL-10 is observed.
Monoclonal Mouse IgG1 Clone # 127107
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-10
Accession # P22301
Supplied in a saline solution containing BSA and Sodium Azide.
PerCP (Peridinin-chlorophyll Protein Complex)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are
available in the Technical Information section on our website.
Detection of IL-10 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes untreated (A) or treated (B) with PMA and Calcium Ionomycin were stained with Mouse Anti-Human IL-10 PerCP-conjugated Monoclonal Antibody (Catalog # IC2172C) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # IC002C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
Interleukin-10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.
Entrez Gene IDs:
3586(Human); 16153(Mouse); 25325(Rat); 397106(Porcine); 403628(Canine); 493683(Feline); 100135553(Guinea Pig); 2949786(Viral)
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
Related Research Areas
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