• Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours or 5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (10 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)
  • Sensitivity
    8 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates), 31.2 - 2,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human IL-11
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human IL-11 Immunoassay is a 4.5-5.0 hour, solid-phase ELISA designed to measure IL-11 in cell culture supernates, serum, or plasma. It contains Sf 21-expressed recombinant human IL-11 and antibodies raised against recombinant human IL-11. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-11 showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-11.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation2.215.350.94.111.473.8

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Standard Deviation1.


The recovery of human IL-11 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 98 90-108
Citrate Plasma (n=5) 105 96-118
EDTA Plasma (n=5) 103 97-110
Heparin Plasma (n=5) 103 93-114
Serum (n=5) 99 89-117
To assess the linearity of the assay, five samples were spiked with high concentrations of IL-11 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-11 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-11
Interleukin-11, also known as AGIF, is a pleiotropic cytokine in the IL-6 family, which also includes LIF, CNTF, Oncostatin M, Cardiotrophin-1, IL-27, and IL-31. It is secreted by osteoblasts, synoviocytes, fibroblasts, chondrocytes, intestinal myofibroblasts, and trophoblasts. It is found in the plasma mainly during inflammation, and is considered to be primarily anti-inflammatory. It stimulates hematopoiesis and thrombopoiesis, regulates macrophage differentiation, and confers mucosal protection in the intestine. It has also been found to enhance T cell polarization toward Th2, promote B cell IgG production, increase osteoclast bone absorption, protect endothelial cells from oxidative stress, and regulate epithelial proliferation and apoptosis. IL-11 signals through a receptor complex that contains IL-11 R alpha and gp130. A soluble form of IL-11 R alpha can bind IL-11 and either form a signaling complex with gp130 on the cell surface or inhibit cell surface IL-11 R alpha/gp130 signaling.
    • Long Name
      Interleukin 11
    • Entrez Gene IDs
      3589 (Human); 16156 (Mouse); 171040 (Rat);
    • Alternate Names
      Adipogenesis inhibitory factor; AGIF; AGIFoprelvekin; IL11; IL-11Oprelvekin; interleukin 11; interleukin-11;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, working standards, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, control, or sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well, and cover with a new plate sealer.
    10.   For Serum & Plasma Samples:  Incubate at room temperature for 2 hours.
      For Cell Culture Supernate Samples:  Incubate at room temperature for 2.5 hours.
    11.   Aspirate and wash 4 times.

    12. 200 µL Substrate Solution
    13.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    14. 50 µL Stop Solution
    15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 15
    Filter your results:

    Sample Type
    1. Improvement of biological and pharmacokinetic features of human interleukin-11 by site-directed mutagenesis.
      Authors: Jung Y, Ahn H, Kim DS, Hwang YR, Ho SH, Kim JM, Kim S, Ma S, Kim S
      Biochem. Biophys. Res. Commun., 2011;405(3):399-404.
      Species: Human
      Sample Type: Plasma
    2. Mechanisms inducing low bone density in duchenne muscular dystrophy in mice and humans.
      Authors: Rufo A, Del Fattore A, Capulli M, Carvello F, De Pasquale L, Ferrari S, Pierroz D, Morandi L, De Simone M, Rucci N, Bertini E, Bianchi ML, De Benedetti F, Teti A
      J. Bone Miner. Res., 2011;26(8):1891-903.
      Species: Human
      Sample Type: Serum
    3. Contributions of TRAIL-mediated megakaryocyte apoptosis to impaired megakaryocyte and platelet production in immune thrombocytopenia.
      Authors: Yang L, Wang L, Zhao CH, Zhu XJ, Hou Y, Jun P, Hou M
      Blood, 2010;116(20):4307-16.
      Species: Human
      Sample Type: Plasma
    4. Helicobacter pylori cytotoxin-associated gene A activates the signal transducer and activator of transcription 3 pathway in vitro and in vivo.
      Authors: Bronte-Tinkew DM, Terebiznik M, Franco A, Ang M, Ahn D, Mimuro H, Sasakawa C, Ropeleski MJ, Peek RM, Jones NL
      Cancer Res., 2009;69(2):632-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo.
      Authors: Siddappa R, Martens A, Doorn J, Leusink A, Olivo C, Licht R, van Rijn L, Gaspar C, Fodde R, Janssen F, van Blitterswijk C, de Boer J
      Proc. Natl. Acad. Sci. U.S.A., 2008;105(20):7281-6.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. Pharmacodynamics and Pharmacokinetics of the Novel Thrombopoietin Mimetic Peptide RWJ-800088 in Humans.
      Authors: Liem-Moolenaar M, Cerneus D, Molloy C, End D, Brown K, de Kam M, Cohen A, van Hensbergen Y, Burggraaf J
      Species: Human
      Sample Type: Plasma
    7. Abnormal cytokine production by bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.
      Authors: Zdzisinska B, Bojarska-Junak A, Dmoszynska A, Kandefer-Szerszen M
      Arch. Immunol. Ther. Exp. (Warsz.), 2008;56(3):207-21.
      Species: Human
      Sample Type: Cell Culture Supernates
    8. Interleukin-11 potentiates oligodendrocyte survival and maturation, and myelin formation.
      Authors: Zhang Y, Taveggia C, Melendez-Vasquez C, Einheber S, Raine CS, Salzer JL, Brosnan CF, John GR
      J. Neurosci., 2006;26(47):12174-85.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. Effects of the active metabolite of leflunomide, A77 1726, on cytokine release and the MAPK signalling pathway in human rheumatoid arthritis synoviocytes.
      Authors: Vergne-Salle&lt;/LastName&gt;&lt;F P&lt;/Initial, Vergne-Salle P, Leger DY, Bertin P, Treves R, Beneytout JL, Liagre B
      Cytokine, 2005;31(5):335-48.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. Relationship between atopic status and nasal interleukin 10 and 11 levels in infants with respiratory syncytial virus bronchiolitis.
      Authors: Chung HL, Kim WT, Kim JK, Choi EJ, Lee JH, Lee GH, Kim SG
      Ann. Allergy Asthma Immunol., 2005;94(2):267-72.
      Species: Human
      Sample Type: Nasal Secretions
    11. Circulating interleukin-6 family cytokines and their receptors in patients with congestive heart failure.
      Authors: Hirota H, Izumi M, Hamaguchi T, Sugiyama S, Murakami E, Kunisada K, Fujio Y, Oshima Y, Nakaoka Y, Yamauchi-Takihara K
      Heart Vessels, 2004;19(5):237-41.
      Species: Human
      Sample Type: Plasma
    12. Effect of azacytidine in the release of leukemia inhibitory factor, oncostatin m, interleukin (IL)-6, and IL-11 by mononuclear cells of patients with refractory anemia.
      Authors: Lopez-Karpovitch X, Barrales-Benitez O, Flores M, Piedras J
      Cytokine, 2002;20(4):154-62.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. Interleukin 11 advances progesterone-induced decidualization of human endometrial stromal cells.
      Authors: Dimitriadis E, Robb L, Salamonsen LA
      Mol. Hum. Reprod., 2002;8(7):636-43.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Interleukin-11 induces Th2 polarization of human CD4(+) T cells.
      Authors: Curti A, Ratta M, Corinti S, Girolomoni G, Ricci F, Tazzari P, Siena M, Grande A, Fogli M, Tura S, Lemoli RM
      Blood, 2001;97(9):2758-63.
      Species: Human
      Sample Type: Cell Culture Supernates
    15. Interleukin-6 is a major effector molecule of short-term G-CSF treatment inducing bone metabolism and an acute-phase response.
      Authors: Carstanjen D, Regenfus M, Muller C, Salama A
      Exp. Hematol., 2001;29(7):812-21.
      Species: Human
      Sample Type: Plasma
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