Human IL-11 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
D1100
Control Products Available
Human IL-11 ELISA Cell Culture Supernate Standard Curve
3 Images
Product Details
Procedure
Citations (21)
FAQs
Supplemental Products
Reviews (1)

Human IL-11 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours or 5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)
Sensitivity
8 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates), 31.2 - 2,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human IL-11
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IL-11 Immunoassay is a 4.5-5.0 hour, solid-phase ELISA designed to measure IL-11 in cell culture supernates, serum, or plasma. It contains Sf 21-expressed recombinant human IL-11 and antibodies raised against recombinant human IL-11. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-11 showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-11.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 70.7 198 565 66.8 188 562
Standard Deviation 1.7 5.2 11.8 3.4 16.8 37.2
CV% 2.4 2.6 2.1 5.1 8.9 6.6

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 50 320 1051 51 305 1018
Standard Deviation 2.2 15.3 50.9 4.1 11.4 73.8
CV% 4.4 4.8 4.8 8 3.7 7.2

Recovery

The recovery of human IL-11 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 98 90-108
Citrate Plasma (n=5) 105 96-118
EDTA Plasma (n=5) 103 97-110
Heparin Plasma (n=5) 103 93-114
Serum (n=5) 99 89-117

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of IL-11 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-11 ELISA Linearity

Data Examples

Human IL-11 ELISA Cell Culture Supernate Standard Curve

Human IL-11 ELISA Serum/Plasma Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-11

Interleukin-11, also known as AGIF, is a pleiotropic cytokine in the IL-6 family, which also includes LIF, CNTF, Oncostatin M, Cardiotrophin-1, IL-27, and IL-31. It is secreted by osteoblasts, synoviocytes, fibroblasts, chondrocytes, intestinal myofibroblasts, and trophoblasts. It is found in the plasma mainly during inflammation, and is considered to be primarily anti-inflammatory. It stimulates hematopoiesis and thrombopoiesis, regulates macrophage differentiation, and confers mucosal protection in the intestine. It has also been found to enhance T cell polarization toward Th2, promote B cell IgG production, increase osteoclast bone absorption, protect endothelial cells from oxidative stress, and regulate epithelial proliferation and apoptosis. IL-11 signals through a receptor complex that contains IL-11 R alpha and gp130. A soluble form of IL-11 R alpha can bind IL-11 and either form a signaling complex with gp130 on the cell surface or inhibit cell surface IL-11 R alpha/gp130 signaling.

Long Name:
Interleukin 11
Entrez Gene IDs:
3589 (Human); 16156 (Mouse); 171040 (Rat)
Alternate Names:
Adipogenesis inhibitory factor; AGIF; AGIFoprelvekin; IL11; IL-11; IL-11Oprelvekin; interleukin 11; interleukin-11; Oprelvekin
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, working standards, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, control, or sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well, and cover with a new plate sealer.
  10.   For Serum & Plasma Samples:  Incubate at room temperature for 2 hours.
    For Cell Culture Supernate Samples:  Incubate at room temperature for 2.5 hours.
  11.   Aspirate and wash 4 times.

  12. 200 µL Substrate Solution
  13.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  14. 50 µL Stop Solution
  15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human IL-11 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Blood Predictive Biomarkers for Patients With Non-small-cell Lung Cancer Associated With Clinical Response to Nivolumab
    Authors: MT Agulló-Ort, Ó Gómez-Mart, S Ponce, L Iglesias, L Ojeda, I Ferrer, I García-Rui, L Paz-Ares, V Pardo-Marq
    Clin Lung Cancer, 2019;0(0):.
    Species: Human
    Sample Types: Plasma
  2. DANCR Promotes Metastasis and Proliferation in Bladder Cancer Cells by Enhancing IL-11-STAT3 Signaling and CCND1 Expression
    Authors: Z Chen, X Chen, R Xie, M Huang, W Dong, J Han, J Zhang, Q Zhou, H Li, J Huang, T Lin
    Mol. Ther., 2019;27(2):326-341.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Decreased interleukin-11 levels in the semen of infertile males
    Authors: L Qian
    Cytokine, 2018;108(0):57-59.
    Species: Human
    Sample Types: Semen
  4. Leukemia inhibitory factor via the Toll-like receptor 5 signaling pathway involves aggravation of cachexia induced by human gastric cancer-derived 85As2 cells in rats
    Authors: K Terawaki, Y Kashiwase, M Uzu, M Nonaka, Y Sawada, K Miyano, Y Higami, K Yanagihara, M Yamamoto, Y Uezono
    Oncotarget, 2018;9(78):34748-34764.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. BMTP-11 is active in preclinical models of human osteosarcoma and a candidate targeted drug for clinical translation
    Authors: VO Lewis, E Devarajan, M Cardó-Vila, DG Thomas, ES Kleinerman, S Marchiò, RL Sidman, R Pasqualini, W Arap
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Autocrine production of IL-11 mediates tumorigenicity in hypoxic cancer cells.
    Authors: Onnis, Barbara, Fer, Nicole, Rapisarda, Annamari, Perez, Victor S, Melillo, Giovanni
    J Clin Invest, 2013;123(4):1615-29.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Improvement of biological and pharmacokinetic features of human interleukin-11 by site-directed mutagenesis.
    Authors: Jung Y, Ahn H, Kim DS, Hwang YR, Ho SH, Kim JM, Kim S, Ma S, Kim S
    Biochem. Biophys. Res. Commun., 2011;405(3):399-404.
    Species: Human
    Sample Types: Plasma
  8. Mechanisms inducing low bone density in duchenne muscular dystrophy in mice and humans.
    Authors: Rufo A, Del Fattore A, Capulli M, Carvello F, De Pasquale L, Ferrari S, Pierroz D, Morandi L, De Simone M, Rucci N, Bertini E, Bianchi ML, De Benedetti F, Teti A
    J. Bone Miner. Res., 2011;26(8):1891-903.
    Species: Human
    Sample Types: Serum
  9. Contributions of TRAIL-mediated megakaryocyte apoptosis to impaired megakaryocyte and platelet production in immune thrombocytopenia.
    Authors: Yang L, Wang L, Zhao CH, Zhu XJ, Hou Y, Jun P, Hou M
    Blood, 2010;116(20):4307-16.
    Species: Human
    Sample Types: Plasma
  10. Helicobacter pylori cytotoxin-associated gene A activates the signal transducer and activator of transcription 3 pathway in vitro and in vivo.
    Authors: Bronte-Tinkew DM, Terebiznik M, Franco A, Ang M, Ahn D, Mimuro H, Sasakawa C, Ropeleski MJ, Peek RM, Jones NL
    Cancer Res., 2009;69(2):632-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Pharmacodynamics and Pharmacokinetics of the Novel Thrombopoietin Mimetic Peptide RWJ-800088 in Humans.
    Authors: Liem-Moolenaar M, Cerneus D, Molloy C, End D, Brown K, de Kam M, Cohen A, van Hensbergen Y, Burggraaf J
    2008;84(0):481.
    Species: Human
    Sample Types: Plasma
  12. cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo.
    Authors: Siddappa R, Martens A, Doorn J, Leusink A, Olivo C, Licht R, van Rijn L, Gaspar C, Fodde R, Janssen F, van Blitterswijk C, de Boer J
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(20):7281-6.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Abnormal cytokine production by bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.
    Authors: Zdzisinska B, Bojarska-Junak A, Dmoszynska A, Kandefer-Szerszen M
    Arch. Immunol. Ther. Exp. (Warsz.), 2008;56(3):207-21.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Interleukin-11 potentiates oligodendrocyte survival and maturation, and myelin formation.
    Authors: Zhang Y, Taveggia C, Melendez-Vasquez C, Einheber S, Raine CS, Salzer JL, Brosnan CF, John GR
    J. Neurosci., 2006;26(47):12174-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Effects of the active metabolite of leflunomide, A77 1726, on cytokine release and the MAPK signalling pathway in human rheumatoid arthritis synoviocytes.
    Authors: Vergne-Salle&lt;/LastName&gt;&lt;F P&lt;/Initial, Vergne-Salle P, Leger DY, Bertin P, Treves R, Beneytout JL, Liagre B
    Cytokine, 2005;31(5):335-48.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Relationship between atopic status and nasal interleukin 10 and 11 levels in infants with respiratory syncytial virus bronchiolitis.
    Authors: Chung HL, Kim WT, Kim JK, Choi EJ, Lee JH, Lee GH, Kim SG
    Ann. Allergy Asthma Immunol., 2005;94(2):267-72.
    Species: Human
    Sample Types: Nasal Secretion
  17. Circulating interleukin-6 family cytokines and their receptors in patients with congestive heart failure.
    Authors: Hirota H, Izumi M, Hamaguchi T, Sugiyama S, Murakami E, Kunisada K, Fujio Y, Oshima Y, Nakaoka Y, Yamauchi-Takihara K
    Heart Vessels, 2004;19(5):237-41.
    Species: Human
    Sample Types: Plasma
  18. Effect of azacytidine in the release of leukemia inhibitory factor, oncostatin m, interleukin (IL)-6, and IL-11 by mononuclear cells of patients with refractory anemia.
    Authors: Lopez-Karpovitch X, Barrales-Benitez O, Flores M, Piedras J
    Cytokine, 2002;20(4):154-62.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Interleukin 11 advances progesterone-induced decidualization of human endometrial stromal cells.
    Authors: Dimitriadis E, Robb L, Salamonsen LA
    Mol. Hum. Reprod., 2002;8(7):636-43.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Interleukin-11 induces Th2 polarization of human CD4(+) T cells.
    Authors: Curti A, Ratta M, Corinti S, Girolomoni G, Ricci F, Tazzari P, Siena M, Grande A, Fogli M, Tura S, Lemoli RM
    Blood, 2001;97(9):2758-63.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Interleukin-6 is a major effector molecule of short-term G-CSF treatment inducing bone metabolism and an acute-phase response.
    Authors: Carstanjen D, Regenfus M, Muller C, Salama A
    Exp. Hematol., 2001;29(7):812-21.
    Species: Human
    Sample Types: Plasma

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Human IL-11 Quantikine ELISA Kit
By Anonymous on 04/07/2020
Application: Sample Tested: Tissue Culture Media