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Human IL-11 Quantikine ELISA Kit

R&D Systems | Catalog # D1100

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Key Product Details

Assay Length

4.5 hours or 5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (10 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL)

Sensitivity

8 pg/mL

Assay Range

15.6-1000 pg/mL (Cell Culture Supernates)
31.2-2000 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
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Product Summary for Human IL-11 Quantikine ELISA Kit

The Quantikine Human IL-11 Immunoassay is a 4.5-5.0 hour, solid-phase ELISA designed to measure IL-11 in cell culture supernates, serum, or plasma. It contains Sf 21-expressed recombinant human IL-11 and antibodies raised against recombinant human IL-11. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-11 showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-11.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human IL-11

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 70.7 198 565 66.8 188 562
Standard Deviation 1.7 5.2 11.8 3.4 16.8 37.2
CV% 2.4 2.6 2.1 5.1 8.9 6.6

Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 50.0 320 1051 51.0 305 1018
Standard Deviation 2.2 15.3 50.9 4.1 11.4 73.8
CV% 4.4 4.8 4.8 8.0 3.7 7.2

Recovery for Human IL-11 Quantikine ELISA Kit

The recovery of human IL-11 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 98 90-108
Citrate Plasma (n=5) 105 96-118
EDTA Plasma (n=5) 103 97-110
Heparin Plasma (n=5) 103 93-114
Serum (n=5) 99 89-117

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of IL-11 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human IL-11 ELISA Linearity

Scientific Data Images for Human IL-11 Quantikine ELISA Kit

Human IL-11 ELISA Cell Culture Supernate Standard Curve

Human IL-11 ELISA Cell Culture Supernate Standard Curve

Human IL-11 ELISA Serum/Plasma Standard Curve

Human IL-11 ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-11

Interleukin-11, also known as AGIF, is a pleiotropic cytokine in the IL-6 family, which also includes LIF, CNTF, Oncostatin M, Cardiotrophin-1, IL-27, and IL-31. It is secreted by osteoblasts, synoviocytes, fibroblasts, chondrocytes, intestinal myofibroblasts, and trophoblasts. It is found in the plasma mainly during inflammation, and is considered to be primarily anti-inflammatory. It stimulates hematopoiesis and thrombopoiesis, regulates macrophage differentiation, and confers mucosal protection in the intestine. It has also been found to enhance T cell polarization toward Th2, promote B cell IgG production, increase osteoclast bone absorption, protect endothelial cells from oxidative stress, and regulate epithelial proliferation and apoptosis. IL-11 signals through a receptor complex that contains IL-11 R alpha and gp130. A soluble form of IL-11 R alpha can bind IL-11 and either form a signaling complex with gp130 on the cell surface or inhibit cell surface IL-11 R alpha/gp130 signaling.

Long Name

Interleukin 11

Alternate Names

AGIF, IL11, Oprelvekin

Entrez Gene IDs

3589 (Human); 16156 (Mouse); 171040 (Rat); 102128313 (Cynomolgus Monkey)

Gene Symbol

IL11

Additional IL-11 Products

Product Documents for Human IL-11 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL-11 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human IL-11 Quantikine ELISA Kit

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Protocols

View specific protocols for Human IL-11 Quantikine ELISA Kit (D1100):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, working standards, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, control, or sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well, and cover with a new plate sealer.
  7.   For Serum & Plasma Samples:  Incubate at room temperature for 2 hours.
    For Cell Culture Supernate Samples:  Incubate at room temperature for 2.5 hours.
  8.   Aspirate and wash 4 times.
    9. 200 µL Substrate Solution
  9.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    10. 50 µL Stop Solution
  10. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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