Human IL-15 Quantikine ELISA Kit

  (36 citations)     
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Product Details
Assay Procedure
Citations (36)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.25 hours or 4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    2 pg/mL
  • Assay Range
    3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human IL-15
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC26 , Quantikine Immunoassay Control Set 934 for Human IL-15 - Please Inquire
Product Summary
The Quantikine Human IL-15 Immunoassay is a 4.25 or 4.5 hour solid phase ELISA designed to measure human IL-15 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-15 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-15 expressed by HuT-102 cells showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-15.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 7.2 22.9 131 9.2 29 154
Standard Deviation 0.3 1.3 5.8 0.7 1.6 10.2
CV% 4.2 5.7 4.4 7.6 5.5 6.6

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 7.7 24.7 140 9.9 32.8 184
Standard Deviation 0.3 1.3 4.8 0.9 2.7 12.2
CV% 3.9 5.3 3.4 9.1 8.2 6.6

Recovery

The recovery of human IL-15 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 98 93-104
EDTA Plasma (n=5) 99 90-112
Heparin Plasma (n=5) 100 91-107
Serum (n=5) 98 92-109
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of IL-15 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line
 IL-15 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-15
IL-15 (Interleukin 15) is a cytokine that induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells. It also induces dendritic cell differentiation and inflammatory activation, exhibits anti-tumor activity, and inhibits the deposition of lipid in adipocytes. Complexes of IL-15 with cell surface IL-15 R alpha interact with complexes of IL-2 R beta and the Common gamma Chain on adjacent cells. This transpresentation mechanism enables cells to respond to IL-15 even if they do not express IL-15 R alpha. Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells. The activity of circulating IL-15 is limited by its association with soluble IL-15 R alpha.
  • Long Name:
    Interleukin 15
  • Entrez Gene IDs:
    3600 (Human); 16168 (Mouse); 25670 (Rat); 493682 (Feline)
  • Alternate Names:
    IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well.
  10.   For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour.
    For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 45 minutes.
  11.   Aspirate and wash 4 times.

  12. 200 µL Substrate Solution
  13.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  14. 50 µL Stop Solution
  15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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Species
Sample Type
  1. Skeletal muscle IL-15/IL-15R? and myofibrillar protein synthesis after resistance exercise
    Authors: A Pérez-Lópe, J McKendry, M Martin-Rin, D Morales-Al, B Pérez-Köhl, D Valadés, J Buján, JAL Calbet, L Breen
    Scand J Med Sci Sports, 2017;0(0):.
    Species: Human
    Sample Type: Serum
  2. Activating and inhibitory receptors on natural killer cells in patients with systemic lupus erythematosis-regulation with interleukin-15
    Authors: SJ Lin, ML Kuo, HS Hsiao, PT Lee, JY Chen, JL Huang
    PLoS ONE, 2017;12(10):e0186223.
    Species: Human
    Sample Type: Serum
  3. Diminished plasma levels of common ?-chain cytokines in pulmonary tuberculosis and reversal following treatment
    Authors: NP Kumar, VV Banurekha, D Nair, S Babu
    PLoS ONE, 2017;12(4):e0176495.
    Species: Human
    Sample Type: Plasma
  4. Involvement of NK Cells and NKp30 Pathway in Antisynthetase Syndrome
    J Immunol, 2016;197(5):1621-30.
    Species: Human
    Sample Type: Serum
  5. Immune Recovery after Allogeneic Hematopoietic Stem Cell Transplantation Following Flu-TBI versus TLI-ATG Conditioning.
    Authors: Hannon M, Beguin Y, Ehx G, Servais S, Seidel L, Graux C, Maertens J, Kerre T, Daulne C, De Bock M, Fillet M, Ory A, Willems E, Gothot A, Humblet-Baron S, Baron F
    Clin Cancer Res, 2015;21(14):3131-9.
    Species: Human
    Sample Type: Plasma
  6. Intramuscular delivery of heterodimeric IL-15 DNA in macaques produces systemic levels of bioactive cytokine inducing proliferation of NK and T cells.
    Authors: Bergamaschi C, Kulkarni V, Rosati M, Alicea C, Jalah R, Chen S, Bear J, Sardesai N, Valentin A, Felber B, Pavlakis G
    Gene Ther, 2015;22(1):76-86.
    Species: Human
    Sample Type: Cell Culture Supernates
  7. The systemic cytokine environment is permanently altered in multiple myeloma.
    Authors: Zheng M, Zhang Z, Bemis K, Belch A, Pilarski L, Shively J, Kirshner J
    PLoS ONE, 2013;8(3):e58504.
    Species: Human
    Sample Type: Plasma
  8. Decreased IL-7 responsiveness is related to oxidative stress in HIV disease.
    Authors: Kalinowska M, Bazdar D, Lederman M, Funderburg N, Sieg S
    PLoS ONE, 2013;8(3):e58764.
    Species: Human
    Sample Type: Serum
  9. IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival.
    Authors: Benito-Miguel M, Garcia-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibanez T, Bonilla-Hernan MG, De Ayala CP, Sanchez-Mateos P, Martin-Mola E, Miranda-Carus ME
    PLoS ONE, 2012;7(7):e40620.
    Species: Human
    Sample Type: Cell Culture Supernates
  10. Development of an IL-15-autocrine CD8 T-cell leukemia in IL-15-transgenic mice requires the cis expression of IL-15R alpha.
    Authors: Sato N, Sabzevari H, Fu S, Ju W, Petrus MN, Bamford RN, Waldmann TA, Tagaya Y
    Blood, 2011;117(15):4032-40.
    Species: Human
    Sample Type: Serum
  11. Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.
    Authors: Giuliani M, Fleury M, Vernochet A, Ketroussi F, Clay D, Azzarone B, Lataillade JJ, Durrbach A
    2011;6(5):e19988.
    Species: Human
    Sample Type: Cell Culture Supernates
  12. Adoptive transfer of autologous natural killer cells leads to high levels of circulating natural killer cells but does not mediate tumor regression.
    Authors: Parkhurst MR, Riley JP, Dudley ME, Rosenberg SA
    Clin. Cancer Res., 2011;17(19):6287-97.
    Species: Human
    Sample Type: Serum
  13. Lack of anti-tumour reactivity despite enhanced numbers of circulating natural killer T cells in two patients with metastatic renal cell carcinoma.
    Authors: Vyth-Dreese FA, Sein J, van de Kasteele W, Dellemijn TA, van den Bogaard C, Nooijen WJ, de Gast GC, Haanen JB, Bex A
    Clin. Exp. Immunol., 2010;162(3):447-59.
    Species: Human
    Sample Type: Plasma
  14. Plasma levels of IL-7 and IL-15 in the first month after myeloablative BMT are predictive biomarkers of both acute GVHD and relapse.
    Authors: Thiant S, Yakoub-Agha I, Magro L, Trauet J, Coiteux V, Jouet JP, Dessaint JP, Labalette M
    Bone Marrow Transplant., 2010;45(10):1546-52.
    Species: Human
    Sample Type: Plasma
  15. Phenotype markers and cytokine intracellular production by CD8+ gammadelta T lymphocytes do not support a regulatory T profile in Behcet's disease patients and healthy controls.
    Authors: Clemente A, Cambra A, Munoz-Saa I, Crespi C, Pallares L, Juan A, Matamoros N, Julia MR
    Immunol. Lett., 2010;129(2):57-63.
    Species: Human
    Sample Type: Serum
  16. Plasma levels of IL-7 and IL-15 after reduced intensity conditioned allo-SCT and relationship to acute GVHD.
    Authors: Thiant S, Labalette M, Trauet J, Coiteux V, de Berranger E, Dessaint JP, Yakoub-Agha I
    Bone Marrow Transplant., 2010;46(10):1374-81.
    Species: Human
    Sample Type: Plasma
  17. Safety and immunologic effects of IL-15 administration in nonhuman primates.
    Authors: Berger C, Berger M, Hackman RC, Gough M, Elliott C, Jensen MC, Riddell SR
    Blood, 2009;114(12):2417-26.
    Species: Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
    Sample Type: Plasma
  18. Enhancing the in vivo expansion of adoptively transferred EBV-specific CTL with lymphodepleting CD45 monoclonal antibodies in NPC patients.
    Authors: Louis CU, Straathof K, Bollard CM, Gerken C, Huls MH, Gresik MV, Wu MF, Weiss HL, Gee AP, Brenner MK, Rooney CM, Heslop HE, Gottschalk S
    Blood, 2009;113(11):2442-50.
    Species: Human
    Sample Type: Plasma
  19. Single-dose nevirapine exposure affects T cell response and cytokine levels in HIV type 1-infected women.
    Authors: Shalekoff S, Meddows-Taylor S, Schramm DB, Gray G, Sherman G, Coovadia A, Kuhn L, Tiemessen CT
    AIDS Res. Hum. Retroviruses, 2009;25(10):1049-53.
    Species: Human
    Sample Type: Plasma
  20. Isolation and characterization of CD8+ regulatory T cells in multiple sclerosis.
    Authors: Correale J, Villa A
    J. Neuroimmunol., 2008;195(1):121-34.
    Species: Human
    Sample Type: Serum
  21. An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation.
    Authors: Dulphy N, Haas P, Busson M, Belhadj S, Peffault de Latour R, Robin M, Carmagnat M, Loiseau P, Tamouza R, Scieux C, Rabian C, Di Santo JP, Charron D, Janin A, Socie G, Toubert A
    J. Immunol., 2008;181(3):2227-37.
    Species: Human
    Sample Type: Serum
  22. Chemokines CXCL10 and CXCL11 in the cerebrospinal fluid of patients with tick-borne encephalitis.
    Authors: Lepej SZ, Misic-Majerus L, Jeren T, Rode OD, Remenar A, Sporec V, Vince A
    Acta Neurol. Scand., 2007;115(2):109-14.
    Species: Human
    Sample Type: CSF
  23. Serum interleukin-15 level is a useful predictor of the complications and mortality in severe acute pancreatitis.
    Authors: Ueda T, Takeyama Y, Yasuda T, Shinzeki M, Sawa H, Nakajima T, Takase K, Matsumoto I, Fujita T, Ajiki T, Fujino Y, Kuroda Y
    Surgery, 2007;142(3):319-26.
    Species: Human
    Sample Type: Serum
  24. Circulating interleukin-15 and RANTES chemokine in Parkinson's disease.
    Authors: Rentzos M, Nikolaou C, Andreadou E, Paraskevas GP, Rombos A, Zoga M, Tsoutsou A, Boufidou F, Kapaki E, Vassilopoulos D
    Acta Neurol. Scand., 2007;116(6):374-9.
    Species: Human
    Sample Type: Serum
  25. Levels of IL-15 in serum and cerebrospinal fluid of patients with Behçet's disease.
    Authors: Hamzaoui K, Hamzaoui A, Ghorbel I, Khanfir M, Houman H
    Scand. J. Immunol., 2006;64(6):655-60.
    Species: Human
    Sample Type: Serum
  26. Juxtacrine function of interleukin-15/interleukin-15 receptor system in tumour derived human B-cell lines.
    Authors: Tsukamoto K, Huang YC, Dorsey WC, Carns B, Sharma V
    Clin. Exp. Immunol., 2006;146(3):559-66.
    Species: Human
    Sample Type: Cell Culture Supernates
  27. Increased ENA-78 in the follicular fluid of patients with endometriosis.
    Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
    Acta Obstet Gynecol Scand, 2006;85(3):336-42.
    Species: Human
    Sample Type: Follicular Fluid
  28. NK cells regulate CD8+ T cell effector function in response to an intracellular pathogen.
    Authors: Vankayalapati R, Klucar P, Wizel B, Weis SE, Samten B, Safi H, Shams H, Barnes PF
    J. Immunol., 2004;172(1):130-7.
    Species: Human
    Sample Type: Cell Culture Supernates
  29. Differential expression of interleukins IL-13 and IL-15 in normal ovarian tissue and ovarian carcinomas.
    Authors: Ripley D, Shoup B, Majewski A
    Gynecol. Oncol., 2004;92(3):761-8.
    Species: Human
    Sample Type: Tissue Homogenates
  30. Differential expression of interleukins IL-13 and IL-15 in normal ovarian tissue and ovarian carcinomas.
    Authors: Ripley D, Shoup B, Majewski A
    Gynecol. Oncol., 2004;92(3):761-8.
    Species: Human
    Sample Type: Tissue Homogenates
  31. Growth and apoptosis of human natural killer cell neoplasms: role of interleukin-2/15 signaling.
    Authors: Yamasaki S, Maeda M, Ohshima K, Kikuchi M, Otsuka T, Harada M
    Leuk. Res., 2004;28(10):1023-31.
    Species: Human
    Sample Type: Serum
  32. Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15.
    Authors: Briard D, Brouty-Boye D, Azzarone B, Jasmin C
    J. Immunol., 2002;168(9):4326-32.
    Species: Human
    Sample Type: Cell Culture Supernates
  33. Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor.
    Authors: Bovolenta C, Pilotti E, Mauri M, Turci M, Ciancianaini P, Fisicaro P, Bertazzoni U, Poli G, Casoli C
    Blood, 2002;99(1):224-31.
    Species: Human
    Sample Type: Cell Culture Supernates
  34. Decreased IL-15 may contribute to elevated IgE and acute inflammation in atopic dermatitis.
    Authors: Ong PY, Hamid QA, Travers JB, Strickland I, Al Kerithy M, Boguniewicz M, Leung DY
    J. Immunol., 2002;168(1):505-10.
    Species: Human
    Sample Type: Cell Culture Supernates
  35. Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
    Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
    J. Exp. Med., 2000;192(2):219-26.
    Species: Human
    Sample Type: Cell Culture Supernates
  36. Interleukin-12 induces sustained activation of multiple host inflammatory mediator systems in chimpanzees.
    Authors: Lauw FN, Dekkers PE, te Velde AA, Speelman P, Levi M, Kurimoto M, Hack CE, Van Deventer SJ, van der Poll T
    J. Infect. Dis., 1999;179(3):646-52.
    Species: Primate - Pan troglodytes (Chimpanzee)
    Sample Type: Plasma
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Quantikine Immunoassay Control Set 934 for Human IL-15

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