Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Western Blot, Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 133617
View all IL-17RA/IL-17R Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-17 R
Leu33-Trp320
Accession # Q96F46

Specificity

Detects human IL-17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL‑17 R, rmIL‑17B R, or recombinant human IL‑17B R is observed. In Western blots, no cross-reactivity with rmIL-17 R is observed

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IL-17RA/IL-17R PE‑conjugated Antibody

Detection of IL-17 R antibody in K562 Human Cell Line antibody by Flow Cytometry.

Detection of IL-17 R in K562 Human Cell Line by Flow Cytometry.

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 R PE-conjugated Monoclonal Antibody (Catalog # FAB177P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of IL-17RA/IL-17R by Western Blot

Detection of IL-17RA/IL-17R by Western Blot

Evaluation of IL-17 signaling in TWIST1-knockdown BC cells compared to control cells. (A) Protein levels of Act1 and IL-17RA in ShCTRL and ShTWIST cells. GAPDH was used to ensure that the same sample amounts were loaded for immunoblotting. Densitometric analysis was done using ImageJ software. (B) Levels of secreted IL-6 and IL-8 proteins in the supernatant of ShCTRL and ShTWIST cells. Data are shown as the mean (±SD). * p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34830027), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human IL-17RA/IL-17R PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: K562 human chronic myelogenous leukemia cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-17RA/IL-17R

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL-17 R is a 120 kDa, 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. Within the ECD, human IL-17 R shares 72% aa sequence identity with mouse and rat IL-17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.

References

  1. Yao, Z. et al. (1997) Cytokine 9:794.
  2. Schwander, R. et al. (2000) J. Exp. Med. 191:1233.

Long Name

Interleukin 17 Receptor

Alternate Names

CD217, Cdw217, IL-17 R, IL-17 RA, IL17RA

Entrez Gene IDs

23765 (Human); 16172 (Mouse); 312679 (Rat); 486759 (Canine); 101925679 (Cynomolgus Monkey)

Gene Symbol

IL17RA

UniProt

Q96F46

Additional IL-17RA/IL-17R Products

Product Documents for Human IL-17RA/IL-17R PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL-17RA/IL-17R PE‑conjugated Antibody

For research use only

Related Research Areas

Cytokines and Receptors Research

Citations for Human IL-17RA/IL-17R PE‑conjugated Antibody

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