Human IL-17B APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human IL-17B in Western blots and ELISAs. In Western blots, approximately 25% cross-reactivity with recombinant mouse IL‑17B is observed and no cross-reactivity with recombinant human (rh) IL‑17, rhIL-17C, rhIL-17E or rhIL-17F is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 174113
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IL-17B
    Accession # Q9UHF5
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑17B in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL‑17B APC‑conjugated Monoclonal Antibody (Catalog # IC1248A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-17B

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2). With the exception of IL-17B, which exists as a non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro‑inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Two receptors (IL‑17 R, and IL-17B R), which are activated by IL-17 family members, have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL‑17 RE, have also been reported (1‑4). Human IL-17B cDNA encodes a 180 amino acid (aa) protein with a putative 20 aa signal peptide (5, 6). Human and mouse IL-17B share 88% amino acid sequence identity. Among IL-17 family members, IL-17B is most closely related to IL-17D, sharing 27% aa sequence homology. IL-17B is expressed highly in spinal cord, and at lower levels in brain, kidney, lung, small intestine, prostate, testes, pancreas, adrenal gland and trachea (5‑7). Expression of IL-17B has also been detected in chondrocytes in articular cartilage (2). IL-17B binds the IL-17B receptor but not IL-17 R and exhibits bioactivities distinct from those of IL-17 (5, 6).

  • References:
    1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
    2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
    3. Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
    4. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
    5. Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.
    6. Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773.
    7. Moore, E.E. et al. (2002) Neuromuscul. Disord. 12:141.
  • Long Name:
    Interleukin 17B
  • Entrez Gene IDs:
    27190 (Human); 56069 (Mouse)
  • Alternate Names:
    Cytokine Zcyto7; cytokine-like protein ZCYTO7; IL17B; IL-17Binterleukin-17 beta; IL20; IL-20interleukin-17B; interleukin 17B; interleukin 20; Interleukin-20; MGC138900; MGC138901; neuronal interleukin-17 related factor; Neuronal interleukin-17-related factor; NIRF; ZCYTO7interleukin-20
Related Research Areas
Isotype Controls
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Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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