Human IL-17D Biotinylated Antibody

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BAF1504
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Human IL-17D Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-17D in ELISAs and Western blots. In sandwich immunoassays, approximately 6% cross‑reactivity with recombinant mouse (rm) IL‑17D is observed and less than 0.02% cross-reactivity with recombinant human (rh) IL‑17, rhIL‑17B, rhIL‑17C, rhIL‑17E, rhIL‑17F, rmIL‑17, and rmIL‑17C is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL‑17D
Ala18-Pro202
Accession # Q8TAD2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑17D (Catalog # 1504-IL)

Human IL-17D Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human IL‑17D Antibody (Catalog # MAB1504)

Standard: Recombinant Human IL-17D Protein (Catalog # 1504-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17D

The Interleukin-17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2). With the exception of IL-17B, which exists as a non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members, have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL‑17 RE, have also been reported (1‑4). The functions of IL-17 RC, D, and E are not known.

Human IL-17D cDNA encodes a 202 amino acid (aa) residues protein with a putative 17 aa signal peptide (5). Human and mouse IL-17D share 78% sequence identity. Among IL-17 family members, IL-17D is most closely related to IL-17B, sharing 27% aa sequence homology (5, 6). IL-17D is expressed preferentially in skeletal muscle, heart, adipose tissue, lung, pancreas, and nervous system (1, 5). Like other IL-17 family members, IL-17D modulates immune responses indirectly by stimulating the production of myeloid growth factors and chemokines including IL-6, IL-8, and GM-CSF (5). IL-17D has also been shown to suppress the proliferation of myeloid progenitors in colony formation assays. The receptor of IL-17D has not yet been identified. However, stimulation of IL-8 production by IL-17D is mediated through the activation of nuclear factor kappa-B (5). The IL-17D preparations from R&D Systems have been found to bind immobilized recombinant IL17B R/Fc in a functional ELISA.

References
  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
  4. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
  5. Starnes, T. et al. (2002) J. Immunol. 169:642.
  6. Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773.
Long Name
Interleukin 17D
Entrez Gene IDs
53342 (Human); 239114 (Mouse)
Alternate Names
FLJ30846; IL17D; IL-17D; IL-17Dinterleukin 27; IL-22; IL-27interleukin-17D; IL27interleukin-27; interleukin 17D; Interleukin-27

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