The Interleukin-17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2). With the exception of IL-17B, which exists as a non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members, have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL‑17 RE, have also been reported (1-4). The functions of IL-17 RC, D, and E are not known. Human IL-17D cDNA encodes a 202 amino acid (aa) residues protein with a putative 17 aa signal peptide (5). Human and mouse IL-17D share 78% sequence identity. Among IL-17 family members, IL-17D is most closely related to IL-17B, sharing 27% aa sequence homology (5, 6). IL-17D is expressed preferentially in skeletal muscle, heart, adipose tissue, lung, pancreas, and nervous system (1, 5). Like other IL-17 family members, IL-17D modulates immune responses indirectly by stimulating the production of myeloid growth factors and chemokines including IL-6, IL-8, and GM-CSF (5). IL-17D has also been shown to suppress the proliferation of myeloid progenitors in colony formation assays. The receptor of IL-17D has not yet been identified. However, stimulation of IL-8 production by IL-17D is mediated through the activation of nuclear factor kappa-B (5). The IL-17D preparations from R&D Systems have been found to bind immobilized recombinant IL17B R/Fc in a functional ELISA.
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Ala18-Pro202
Accession # Q8TAD2.1
Specificity
Clonality
Host
Isotype
Endotoxin Level
Applications for Human IL‑17D Antibody
Blockade of Receptor-ligand Interaction
In a functional ELISA, 3-9 µg/mL of this antibody will block 50% of the binding of 2.5 μg/mL of biotinylated Recombinant Human IL-17B R Fc Chimera to immobilized Recombinant Human IL-17D (Catalog # 1504-IL) coated at 200 ng/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.
Western Blot
Sample: Recombinant Human IL‑17D (Catalog # 1504-IL)
under non-reducing conditions only
Human IL-17D Sandwich Immunoassay
Reviewed Applications
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Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-17D
References
- Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
- Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
- Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
- Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
- Starnes, T. et al. (2002) J. Immunol. 169:642.
- Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional IL-17D Products
Product Documents for Human IL‑17D Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IL‑17D Antibody
For research use only
Related Research Areas
Customer Reviews for Human IL‑17D Antibody (1)
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Application: MicroarraySample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 12/13/2019Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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