Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 70625
View all IL-18 R alpha/IL-1 R5 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5
Glu20-Arg329
Accession # Q13478

Specificity

Detects human IL-18 R alpha /IL-1 R5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL‑1 R3, rhIL-1 R4, rhIL-1 R6, rhIL-1 R7, rhIL-1 R8, rhIL-1 R9, rhSIGIRR or recombinant mouse IL-18 R alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IL‑18 R alpha /IL‑1 R5 PE‑conjugated Antibody

Detection of IL-18 Ra/IL-1 R5 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of IL‑18 R alpha /IL‑1 R5 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) treated with 10 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) and 5 µg/mL PHA for 48 hours were stained with Mouse Anti-Human IL-18 Ra/IL-1 R5 PE-conjugated Monoclonal Antibody (Catalog # FAB840P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of IL-18 R alpha /IL-1 R5 by Flow Cytometry

Detection of IL-18 R alpha /IL-1 R5 by Flow Cytometry

IL-18 promotes T-cell acute lymphoblastic leukaemia (T-ALL) growth in vitro. A Cell surface expression of IL-18R alpha (left panel) and IL-18R beta (right panel) in M106 and M114 T-ALL cells. Histograms show the mean fluorescence intensity (MFI) of IL-18R alpha and IL-18R beta staining in, respectively, 7 and 11 T-ALL samples. Control: T-ALL or UCB CD7+ T cells stained with isotype control antibodies. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24778454), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human IL‑18 R alpha /IL‑1 R5 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with Recombinant Human IL‑2 (Catalog # 202-IL) and PHA

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-18 R alpha/IL-1 R5

Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also known as IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also known as IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL‑18 R beta is required for high affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.

References

  1. Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
  2. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
  3. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.

Long Name

Interleukin 18 Receptor alpha

Alternate Names

CD218a, IL-1 R5, IL-1 Rrp, IL-18 R1, IL-18Ra, IL18R alpha, IL18R1

Entrez Gene IDs

8809 (Human); 16182 (Mouse)

Gene Symbol

IL18R1

UniProt

Q13478

Additional IL-18 R alpha/IL-1 R5 Products

Product Documents for Human IL‑18 R alpha /IL‑1 R5 PE‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human IL‑18 R alpha /IL‑1 R5 PE‑conjugated Antibody

For research use only

Citations for Human IL‑18 R alpha /IL‑1 R5 PE‑conjugated Antibody

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