Human IL-18 R alpha /IL-1 R5 Antibody

Catalog # Availability Size / Price Qty
MAB840-SP
MAB840-500
MAB840-100
IFN‑ gamma  Secretion Induced by IL‑18/IL‑1F4 and Neutralization by IL‑18 R alpha /IL‑1 R5 Antibody.
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Citations (9)
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Human IL-18 R alpha /IL-1 R5 Antibody Summary

Species Reactivity
Human
Specificity
Detects Recombinant Human IL-18 R alpha /IL-1 R5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-1 R3, rhIL-1 R4, rhIL-1 R6, rhIL-1 R7, rhIL-1 R8, rhIL-1 R9, rhSIGIRR or recombinant mouse IL-18 R alpha is observed.
Source
Monoclonal Mouse IgG1 Clone # 70625
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5
Glu20-Arg329
Accession # Q13478
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood mononuclear cells treated with PHA and Recombinant Human IL‑2 (Catalog # 202-IL)
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human skin
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in KG-1 human myeloid leukemia cells. The Neutralization Dose (ND50) is typically 0.03-0.1 µg/mL in the presence of 40 ng/mL Recombinant Human IL‑18/IL‑1F4 and 20 ng/mL Recombinant Human TNF-alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Neutralization IFN‑ gamma  Secretion Induced by IL‑18/IL‑1F4 and Neutrali-zation by IL‑18 R alpha /IL‑1 R5 Antibody. View Larger

IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutralization by IL‑18 R alpha /IL‑1 R5 Antibody. In the presence of Recombinant Human TNF-alpha (20 ng/mL, Catalog # 210-TA), Recombinant Human IL-18/IL-1F4 (Catalog # B003-5) stimulates IFN-gamma secretion in KG-1 human myeloid leukemia cells in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). Under these conditions, IFN-gamma secretion elicited by Recombinant Human IL-18/IL-1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-18 Ra/ IL-1 R5 Monoclonal Antibody (Catalog # MAB840). The ND50 is typically 0.03-0.1 µg/mL.

Immunocytochemistry IL‑18 Ra/IL‑1 R5 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IL‑18 R alpha /IL‑1 R5 in Human PBMCs. IL-18 Ra/IL-1 R5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Human IL-18 Ra/IL-1 R5 Mono-clonal Antibody (Catalog # MAB840) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-18 R alpha/IL-1 R5

Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL‑18 R beta is required for high affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.

References
  1. Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
  2. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
  3. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
Long Name
Interleukin 18 Receptor alpha
Entrez Gene IDs
8809 (Human); 16182 (Mouse)
Alternate Names
CD218a; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL18R alpha; IL18R1; IL-18Ra

Product Datasheets

Citations for Human IL-18 R alpha /IL-1 R5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma
    Sci Rep, 2016;6(0):26757.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. Interleukin 18 function in atherosclerosis is mediated by the interleukin 18 receptor and the Na-Cl co-transporter.
    Authors: Wang J, Sun C, Gerdes N, Liu C, Liao M, Liu J, Shi M, He A, Zhou Y, Sukhova G, Chen H, Cheng X, Kuzuya M, Murohara T, Zhang J, Cheng X, Jiang M, Shull G, Rogers S, Yang C, Ke Q, Jelen S, Bindels R, Ellison D, Jarolim P, Libby P, Shi G
    Nat Med, 2015;21(7):820-6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. Regulation of development of CD56 bright CD11c + NK-like cells with helper function by IL-18.
    Authors: Li W, Okuda A, Yamamoto H, Yamanishi K, Terada N, Yamanishi H, Tanaka Y, Okamura H
    PLoS ONE, 2013;8(12):e82586.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. IL-1 Family Members IL-18 and IL-33 Upregulate the Inflammatory Potential of Differentiated Human Th1 and Th2 Cultures.
    Authors: Blom L, Poulsen L
    J Immunol, 2012;189(9):4331-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis.
    Authors: Takei S, Hoshino T, Matsunaga K, Sakazaki Y, Sawada M, Oda H, Takenaka S, Imaoka H, Kinoshita T, Honda S, Ida H, Fukuda TA, Aizawa H
    Arthritis Res. Ther., 2011;13(2):R52.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot
  6. Glomerular accumulation of plasmacytoid dendritic cells in active lupus nephritis: role of interleukin-18.
    Authors: Tucci M, Quatraro C, Lombardi L, Pellegrino C, Dammacco F, Silvestris F
    Arthritis Rheum., 2008;58(1):251-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
    Authors: Rao DA, Tracey KJ, Pober JS
    J. Immunol., 2007;179(10):6536-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. CD molecules 2006--human cell differentiation molecules.
    Authors: Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
    J. Immunol. Methods, 2007;319(1):1-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006;241(1):7-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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