Human IL-18 R alpha /IL-1 R5 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB840A-100
FAB840A-025

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Detection of IL‑18 R alpha /IL‑1 R5 in Human PBMCs by Flow Cytometry.
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Citations (3)
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Human IL-18 R alpha /IL-1 R5 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-18 R alpha /IL-1 R5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL‑1 R3, rhIL-1 R4, rhIL-1 R6, rhIL-1 R7, rhIL-1 R8, rhIL-1 R9, rhSIGIRR or recombinant mouse IL-18 R alpha is observed.
Source
Monoclonal Mouse IgG1 Clone # 70625
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5
Glu20-Arg329
Accession # Q13478
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL‑18 Ra/IL‑1 R5 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of IL‑18 R alpha /IL‑1 R5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PHA (5 µg/ml) and Recombinant Human IL-2 (Catalog # 202-IL;10 ng/ml) for 2 days were stained with Mouse Anti-Human IL-18 Ra/IL-1 R5 APC-conjugated Monoclonal Antibody (Catalog # FAB840A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-18 R alpha/IL-1 R5

Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also known as IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also known as IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL‑18 R beta is required for high affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.

References
  1. Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
  2. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
  3. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
Long Name
Interleukin 18 Receptor alpha
Entrez Gene IDs
8809 (Human); 16182 (Mouse)
Alternate Names
CD218a; CD218a;CDw218a;IL18R1;IL18RA;IL-18Ralpha;IL-18R-alpha;IL18Ralpha2;IL1RRP;IL-1Rrp;Interleukin-18 receptor 1; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL18R alpha; IL18R1; IL-18Ra

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Citations for Human IL-18 R alpha /IL-1 R5 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Altered expression of IL-18 binding protein and IL-18 receptor in basophils and mast cells of asthma patients
    Authors: Z Wang, Z Liu, L Wang, J Wang, L Chen, H Xie, H Zhang, S He
    Scand. J. Immunol., 2018-04-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Role of IL-18 in atopic asthma is determined by balance of IL-18/IL-18BP/IL-18R
    Authors: H Zhang, J Wang, L Wang, H Xie, L Chen, S He
    J. Cell. Mol. Med., 2017-09-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Enhanced Expression of IL-18 and IL-18BP in Plasma of Patients with Eczema: Altered Expression of IL-18BP and IL-18 Receptor on Mast Cells
    Authors: Y Hu, J Wang, H Zhang, H Xie, W Song, Q Jiang, N Zhao, S He
    Mediators Inflamm., 2017-08-03;2017(0):3090782.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human IL-18 R alpha /IL-1 R5 APC-conjugated Antibody

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Human IL-18 R alpha /IL-1 R5 APC-conjugated Antibody
By Anonymous on 05/31/2016
Application: Flow Sample Tested: Peripheral blood monocytes,Peripheral blood T cells Species: Human

Detection of IL18Rα/IL-1R5 expression in lymphocytes and monocytes by flow cytometry: Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll gradient method from freshly drawn blood and stained for IL18Ralpha using the R&D mouse Anti-human IL-18alpha/IL-1R5-APC antibody (cat# FAB840A). As suggested by the manufacturer, 10ul of antibody was used per 10e6 cells. After gating on CD3+ lymphocytes (Fig.1a) and CD14+ monocytes (Fig.1b), IL-18Ralpha expression on T lymphocytes and monocytes were analyzed. Panel 1 shows Fluorescence Minus One (FMO) which served as negative control; panel 2 shows IL18alpha+ cells; panel 3 shows overlay of panel 1 and panel 2.