Human IL-18 R alpha /IL-1 R5 APC-conjugated Antibody

(1 Review)
  • Species Reactivity
  • Specificity
    Detects human IL-18 R alpha /IL-1 R5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL‑1 R3, rhIL-1 R4, rhIL-1 R6, rhIL-1 R7, rhIL-1 R8, rhIL-1 R9, rhSIGIRR or recombinant mouse IL-18 R alpha is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 70625
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5
    Accession # Q13478
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑18 R alpha /IL‑1 R5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PHA (5 μg/ml) and Recombinant Human IL‑2 (Catalog # 202-IL;10 ng/ml) for 2 days were stained with Mouse Anti-Human IL‑18 R alpha /IL‑1 R5 APC‑conjugated Monoclonal Antibody (Catalog # FAB840A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-18 R alpha/IL-1 R5

Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also known as IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also known as IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL‑18 R beta is required for high affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.

  • References:
    1. Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
    2. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
    3. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
  • Long Name:
    Interleukin 18 Receptor alpha
  • Entrez Gene IDs:
    8809 (Human); 16174 (Mouse)
  • Alternate Names:
    CD218a; IL-1 R5; IL-1 Rrp; IL-18 R1; IL18R alpha; IL18R1; IL-18Ra
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 5  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Human IL-18 R alpha /IL-1 R5 APC-conjugated Antibody FAB840A
 Flow Human Anonymous 05/31/2016
Image Details
Flow Cytometry Human IL-18 R alpha /IL-1 R5 APC-conjugated Antibody FAB840A
Flow Cytometry: Human IL-18 R alpha /IL-1 R5 APC-conjugated Antibody [FAB840A]


ApplicationFlow Cytometry
Sample TestedPeripheral blood monocytes,Peripheral blood T cells

Other Experimental Details

Other Experimental DetailsDetection of IL18R alpha /IL-1R5 expression in lymphocytes and monocytes by flow cytometry: Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll gradient method from freshly drawn blood and stained for IL18Ralpha using the R&D mouse Anti-human IL-18alpha/IL-1R5-APC antibody (cat# FAB840A). As suggested by the manufacturer, 10ul of antibody was used per 10e6 cells. After gating on CD3+ lymphocytes (Fig.1a) and CD14+ monocytes (Fig.1b), IL-18Ralpha expression on T lymphocytes and monocytes were analyzed. Panel 1 shows Fluorescence Minus One (FMO) which served as negative control; panel 2 shows IL18alpha+ cells; panel 3 shows overlay of panel 1 and panel 2.

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