Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 70625
View all IL-18 R alpha/IL-1 R5 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5
Glu20-Arg329
Accession # Q13478

Specificity

Detects human IL-18 R alpha /IL-1 R5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL‑1 R3, rhIL-1 R4, rhIL-1 R6, rhIL-1 R7, rhIL-1 R8, rhIL-1 R9, rhSIGIRR or recombinant mouse IL-18 R alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IL‑18 R alpha /IL‑1 R5 APC‑conjugated Antibody

Detection of IL‑18 Ra/IL‑1 R5 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of IL‑18 R alpha /IL‑1 R5 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) treated with PHA (5 µg/ml) and Recombinant Human IL-2 (Catalog # 202-IL;10 ng/ml) for 2 days were stained with Mouse Anti-Human IL-18 Ra/IL-1 R5 APC-conjugated Monoclonal Antibody (Catalog # FAB840A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IL‑18 R alpha /IL‑1 R5 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PHA and Recombinant Human IL‑2 (Catalog # 202-IL)

Reviewed Applications

Read 1 review rated 5 using FAB840A in the following applications:

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-18 R alpha/IL-1 R5

Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also known as IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also known as IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL‑18 R beta is required for high affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.

References

  1. Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
  2. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
  3. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.

Long Name

Interleukin 18 Receptor alpha

Alternate Names

CD218a, IL-1 R5, IL-1 Rrp, IL-18 R1, IL-18Ra, IL18R alpha, IL18R1

Entrez Gene IDs

8809 (Human); 16182 (Mouse)

Gene Symbol

IL18R1

UniProt

Q13478

Additional IL-18 R alpha/IL-1 R5 Products

Product Documents for Human IL‑18 R alpha /IL‑1 R5 APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human IL‑18 R alpha /IL‑1 R5 APC‑conjugated Antibody

For research use only

Citations for Human IL‑18 R alpha /IL‑1 R5 APC‑conjugated Antibody

Customer Reviews for Human IL‑18 R alpha /IL‑1 R5 APC‑conjugated Antibody (1)

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  • Human IL-18 R alpha /IL-1 R5 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood T cells and Peripheral blood monocytes
    Species: Human
    Verified Customer | Posted 05/31/2016
    Detection of IL18R alpha /IL-1R5 expression in lymphocytes and monocytes by flow cytometry: Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll gradient method from freshly drawn blood and stained for IL18Ralpha using the R&D mouse Anti-human IL-18alpha/IL-1R5-APC antibody (cat# FAB840A). As suggested by the manufacturer, 10ul of antibody was used per 10e6 cells. After gating on CD3+ lymphocytes (Fig.1a) and CD14+ monocytes (Fig.1b), IL-18Ralpha expression on T lymphocytes and monocytes were analyzed. Panel 1 shows Fluorescence Minus One (FMO) which served as negative control; panel 2 shows IL18alpha+ cells; panel 3 shows overlay of panel 1 and panel 2.
    Human IL‑18 R alpha /IL‑1 R5 APC‑conjugated Antibody FAB840A

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