Human IL-19 Fluorescein-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human IL-19 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 152112
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IL-19
    Accession # AAN40906
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑19 in Human Peripheral Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes cultured with LPS and monensin were stained with Mouse Anti-Human IL‑19 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC1035F) and Mouse Anti-Human CD163 PE‑conjugated Monoclonal Antibody (Catalog # FAB1607P). Quadrant markers were set based on control antibody staining (Catalog # IC0041F). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-19

Human Interleukin 19 (IL-19) is a member of the IL-10 family of related cytokines. Its gene contains two alternate translation initiation sites, generating precursors of 215 amino acids (aa) and 177 aa, respectively. Both isoforms are processed to 17 kDa, 153 aa mature molecules. IL-19 contains seven helices and is secreted as a 35 kDa monomer. There are two potential N-linked glycosylation sites, and it is likely that the molecule is glycosylated. Mature human IL-19 shares 69% aa sequence identity with the mature mouse homologue. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells. IL-19 expression is limited to activated keratinocytes and monocytes. IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (IL-20 R alpha, also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R beta or IL-20 R2). This receptor complex is also shared by IL-20 and IL-24. Functionally, IL-19 induces IL-6 and TNF-alpha production by monocytes, and drives T-helper cell differentiation towards a Th2 response (1‑5).

  • References:
    1. Gallagher, G. et al. (2000) Genes Immun. 1:442.
    2. Gallagher, G. et al. (2004) Int. Immunopharmacol. 4:615.
    3. Laio, Y-C. et al. (2002) J. Immunol. 169:4288.
    4. Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306.
    5. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  • Long Name:
    Interleukin 19
  • Entrez Gene IDs:
    29949 (Human); 329244 (Mouse)
  • Alternate Names:
    IL-10C; IL19; IL-19; interleukin 19; MDA1; melanoma differentiation associated protein-like protein; NG.1Melanoma differentiation-associated protein-like protein; ZMDA1interleukin-19
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Fluorescein‑conjugated Control Antibody

Ctrl IC0041F 6  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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