Human IL-1 alpha /IL-1F1 Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
IC200F
Human IL-1 alpha /IL-1F1 Fluorescein-conjugated Antibody in Data
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Human IL-1 alpha /IL-1F1 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-1 alpha /IL-1F1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) IL-1 beta, -2, -3, -4, -5, -6, -7, -8, -9, -10, -11, -12, -13, rmIL-1 alpha, -1 beta, -3, -4, -5, -6, -7, -9, or -13.
Source
Monoclonal Mouse IgG2A Clone # 4414
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-1 alpha /IL-1F1
Ser113-Ala271
Accession # P01583
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of IL‑1 alpha /IL‑1F1 in Human Blood Monocytes by Flow Cytometry. View Larger

Detection of IL‑1 alpha /IL‑1F1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes (A) treated with LPS were stained with Mouse Anti-Human IL-1a/IL-1F1 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC200F) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on control antibody staining (Catalog # IC002F). (B) Inhibition of Mouse Anti-Human IL-1a/IL-1F1 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC200F) staining by the addition of excess Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-1 alpha/IL-1F1

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts, and glia (oligodendroglia, astrocytes, and microglia).

IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.

Long Name
Interleukin 1 alpha
Entrez Gene IDs
3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)
Alternate Names
BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha

Product Datasheets

Citation for Human IL-1 alpha /IL-1F1 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The pro-inflammatory phenotype of the human non-classical monocyte subset is attributed to senescence
    Authors: SM Ong, E Hadadi, TM Dang, WH Yeap, CT Tan, TP Ng, A Larbi, SC Wong
    Cell Death Dis, 2018;9(3):266.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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