Human IL-1 alpha /IL-1F1 Membrane Form Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB200F
Human IL-1 alpha /IL-1F1 Membrane Form Fluorescein-conjugated Antibody in Data
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Human IL-1 alpha /IL-1F1 Membrane Form Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑1 alpha /IL‑1F1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-1 alpha, recombinant rat IL-1 alpha, recombinant cotton rat IL-1 alpha, recombinant human (rh) IL-1 beta or rhIL-18 is observed.
Source
Monoclonal Mouse IgG1 Clone # 3405
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human IL‑1 alpha /IL‑1F1
Ser113-Ala271
Accession # P01583
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of IL‑1 alpha /IL‑1F1 in Human PBMCs by Flow Cytometry. View Larger

Detection of IL‑1 alpha /IL‑1F1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with LPS for 24 hours were stained with Mouse Anti-Human IL-1a/IL-1F1 Membrane Form Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB200F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-1 alpha/IL-1F1

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia). IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.

Long Name
Interleukin 1 alpha
Entrez Gene IDs
3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)
Alternate Names
BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha

Product Datasheets

Citations for Human IL-1 alpha /IL-1F1 Membrane Form Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. The pro-inflammatory phenotype of the human non-classical monocyte subset is attributed to senescence
    Authors: SM Ong, E Hadadi, TM Dang, WH Yeap, CT Tan, TP Ng, A Larbi, SC Wong
    Cell Death Dis, 2018;9(3):266.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. IL-34- and M-CSF-induced macrophages switch memory T cells into Th17 cells via membrane IL-1alpha.
    Authors: Foucher E, Blanchard S, Preisser L, Descamps P, Ifrah N, Delneste Y, Jeannin P
    Eur J Immunol, 2015;45(4):1092-102.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. MTOR regulates the pro-tumorigenic senescence-associated secretory phenotype by promoting IL1A translation.
    Authors: Laberge R, Sun Y, Orjalo A, Patil C, Freund A, Zhou L, Curran S, Davalos A, Wilson-Edell K, Liu S, Limbad C, Demaria M, Li P, Hubbard G, Ikeno Y, Javors M, Desprez P, Benz C, Kapahi P, Nelson P, Campisi J
    Nat Cell Biol, 2015;17(8):1049-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human IL-1 alpha /IL-1F1 Membrane Form Fluorescein-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human IL-1 alpha /IL-1F1 Membrane Form Fluorescein-conjugated Antibody and earn rewards!

Have you used Human IL-1 alpha /IL-1F1 Membrane Form Fluorescein-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review