Human IL-1 beta /IL-1F2 PE-conjugated Antibody

Recombinant Monoclonal Antibody..
  • Species Reactivity
  • Specificity
    Detects human IL‑1 beta /IL‑1F2 by direct ELISAs.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1027B
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    E. coli-derived recombinant human IL‑1 beta /IL‑1F2
    Accession # P01584
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑1 beta /IL‑1F2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes either (A) untreated or (B) treated with 250 ng/mL LPS overnight were stained with Rabbit Anti-Human IL‑1 beta /IL‑1F2 PE‑conjugated Monoclonal Antibody (Catalog # IC8406P) and Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A). Quadrant markers were set based on control antibody staining (Catalog # IC105P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-1 beta/IL-1F2

IL-1 is a common name for two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) sequence identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and may exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R Accessory Protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The human IL-1 beta cDNA encodes a 269 aa precursor that contains a 116 aa propeptide which is cleaved intracellularly by the cysteine protease IL-1 beta -converting Enzyme (Caspase-1/ICE) to generate an active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus macaque, and 67-78% aa sequence identity with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

  • References:
    1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
    2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
    3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
    4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
    5. March, C.J. et al. (1985) Nature 315:641.
    6. Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. USA 81:7907.
    7. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
  • Long Name:
    Interleukin 1 beta
  • Entrez Gene IDs:
    3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
  • Alternate Names:
    catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1B; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG PE-conjugated Control

Ctrl IC105P
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Rabbit IgG PE-conjugated Antibody

Ctrl IC1051P
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