Human IL-2 DuoSet ELISA

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Catalog # Availability Size / Price Qty
DY202
DY202-05
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Human IL-2 ELISA Standard Curve
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Product Details
Procedure
Citations (66)
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Human IL-2 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
15.6 - 1,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IL-2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Block Buffer: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Reagent Diluent: 0.1% BSA, 0.05% Tween 20 in Tris-buffered Saline (20 mM Trizma base, 150 mM NaCI) pH 7.2-7.4, 0.2 μm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

 

Scientific Data

Human IL-2 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-2

Interleukin 2 (IL-2), also known as T cell growth factor (TCGF), is a 15-18 kDa variably glycosylated alpha -helical polypeptide that is a member of the Common gamma Chain ( gamma c) cytokine family (1-4). It exists as a monomer and has a notably short half-life (< 30 minutes) (1). Human IL-2 is synthesized as a 153 amino acid (aa) precursor that contains a 20 aa signal sequence plus a 133 aa mature region (5, 6). The mature region is alpha -helical in nature, and contains one utilized O-linked glycosylation site at Thr3 plus three cysteines, two of which form an intrachain disulfide bond that is essential for activity (7). Mature human IL-2 shares 73%, 66%, 78% and 97% aa identity with canine, rat, feline and rhesus monkey IL-2, respectively. Although human IL-2 shares only approximately 60% aa identity with the highly polymorphic mouse IL-2, human IL-2 is known to be active on mouse IL-2 responsive cells. Cells reported to secrete IL-2 include gamma δ T cells (8), activated conventional CD4+ and CD8+ T cells (1, 9), neurons (10, 11), microglia (12), and hematopoietic stem cells (13). 

The receptor for IL-2 (IL-2 R) is composed of three subunits, the 55 kDa CD25/IL-2 R alpha chain, the 70 kDa IL-2 R beta chain, and the 65 kDa Common gamma Chain (1, 3). IL-2 first binds to CD25, the binary complex then recruits IL-2 R beta and gamma c to form the quaternary signaling complex (1, 14). In addition to IL-2, IL-2 R beta is used by IL-15 in its quaternary signaling complex. gamma c also serves as a signaling receptor for IL-4, -7, -9, -15, and -21 (1, 3). 
In vitro studies have shown an important role for IL-2 in T cell activation and expansion. In vivo, IL-2 is critical for the development, maintenance and function of regulatory T cells (Treg) which provide protection against autoimmune disease. On the other hand, IL-2 can also promote autoimmune inflammation in target organs through its roles in regulating the expression of T cell trafficking genes, and production of Th2 cytokines. Within the CD8+ T cell subset, IL-2 is essential for optimal primary responses and differentiation into terminal effector cells. IL-2 also promotes the development of activated CD8+ T cells into memory cells. (1).

Long Name:
Interleukin 2
Entrez Gene IDs:
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names:
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL of Block Buffer to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human IL-2 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

66 Citations: Showing 1 - 10
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  1. Discovery of potent and selective HPK1 inhibitors based on the 2,4-disubstituted pyrimidine scaffold with immune modulatory properties for ameliorating T cell exhaustion
    Authors: Zeng, S;Zeng, M;Yuan, S;He, L;Jin, Y;Huang, J;Zhang, M;Yang, M;Pan, Y;Wang, Z;Chen, Y;Xu, X;Huang, W;
    Bioorganic chemistry
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Inflammatory biomarkers at different stages of Sarcopenia in older women
    Authors: da Costa Teixeira, LA;Avelar, NCP;Peixoto, MFD;Parentoni, AN;Santos, JMD;Pereira, FSM;Danielewicz, AL;Leopoldino, AAO;Costa, SP;Arrieiro, AN;Soares, LA;da Silva Lage, VK;Prates, ACN;Taiar, R;de Carvalho Bastone, A;Oliveira, VC;Oliveira, MX;Costa, HS;Nobre, JNP;Brant, FP;Duarte, TC;Figueiredo, PHS;Mendonça, VA;Lacerda, ACR;
    Scientific reports
    Species: Human
    Sample Types: Plasma
  3. T Cells Expressing a Modified FcgammaRI Exert Antibody-Dependent Cytotoxicity and Overcome the Limitations of CAR T-cell Therapy against Solid Tumors
    Authors: D Rasoulouni, N Santana-Ma, A Gutwillig, L Farhat-You, L Tal, S Amar, M Milyavsky, SSNA Muddineni, N Solomon, H Shpilt, S Dotan, N Pilpel, C Waskow, M Feinmesser, P Rider, Y Carmi
    Cancer Immunology Research, 2023-06-02;0(0):OF1-OF18.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Characterization of 405B8H3(D-E), a newly engineered high affinity chimeric LAG-3 antibody with potent antitumor activity
    Authors: Lan, X;Yang, TTC;Wang, Y;Qu, B;Rong, S;Song, N;
    FEBS open bio
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Combinatorial inhibition of Tec kinases BTK and ITK is beneficial in ameliorating murine sclerodermatous chronic graft versus host disease
    Authors: Palaniyandi, S;Strattan, E;Kumari, R;Mysinger, M;Hakim, N;Kesler, MV;Apatira, M;Bittencourt, F;Wang, L;Jia, Z;Gururaja, TL;Hill, RJ;Hildebrandt, GC;
    Bone marrow transplantation
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Adiponectin Is a Contributing Factor of Low Appendicular Lean Mass in Older Community-Dwelling Women: A Cross-Sectional Study
    Authors: LAC Teixeira, JM Dos Santos, AN Parentoni, LP Lima, TC Duarte, FP Brant, CDC Neves, FSM Pereira, NCP Avelar, AL Danielewic, AAO Leopoldino, SP Costa, AN Arrieiro, LA Soares, ACN Prates, JNP Nobre, A de Carvalh, VC de Oliveir, MX Oliveira, PH Scheidt Fi, HS Costa, V Amaral Men, R Taiar, AC Rodrigues
    Journal of Clinical Medicine, 2022-12-02;11(23):.
    Species: Human
    Sample Types: Plasma
  7. Co-expression IL-15 receptor alpha with IL-15 reduces toxicity via limiting IL-15 systemic exposure during CAR-T immunotherapy
    Authors: Y Zhang, Q Zhuang, F Wang, C Zhang, C Xu, A Gu, WH Zhong, Y Hu, X Zhong
    Oncogene, 2022-09-27;20(1):432.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Generation and Functional Characterization of PLAP CAR-T Cells against Cervical Cancer Cells
    Authors: V Yekehfalla, S Pahlavanne, A Sayadmanes, Z Momtahan, B Ma, M Basiri
    Biomolecules, 2022-09-14;12(9):.
    Species: Human
    Sample Types: Cell Culture Supernatants
  9. SARS-CoV-2 Spike Does Not Possess Intrinsic Superantigen-like Inflammatory Activity
    Authors: C Amormino, V Tedeschi, G Paldino, S Arcieri, MT Fiorillo, A Paiardini, L Tuosto, M Kunkl
    Cells, 2022-08-15;11(16):.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. HydrAd: A Helper-Dependent Adenovirus Targeting Multiple Immune Pathways for Cancer Immunotherapy
    Authors: A Rosewell S, C Porter, G Biegert, L Jatta, M Suzuki
    Oncogene, 2022-06-02;14(11):.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Novel Bi-Specific Immuno-Modulatory Tribodies Potentiate T Cell Activation and Increase Anti-Tumor Efficacy
    Authors: M Passariell, A Yoshioka, K Takahashi, SI Hashimoto, R Rapuano Le, L Manna, K Nakamura, C De Lorenzo
    International Journal of Molecular Sciences, 2022-03-23;23(7):.
    Species: Human
    Sample Types: Cell Culture Supernatents
  12. Activation of the Innate Immune Checkpoint CLEC5A on Myeloid Cells in the Absence of Danger Signals Modulates Macrophages' Function but Does Not Trigger the Adaptive T Cell Immune Response
    Authors: MJ Tosiek, K Groesser, A Pekcec, M Zwirek, G Murugesan, E Borges
    Journal of Immunology Research, 2022-02-25;2022(0):9926305.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Novel Combinations of Human Immunomodulatory mAbs Lacking Cardiotoxic Effects for Therapy of TNBC
    Authors: C Vetrei, M Passariell, G Froechlich, R Rapuano Le, E Sasso, N Zambrano, C De Lorenzo
    Cancers, 2021-12-27;14(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Discovery of a novel anti PD-L1 X TIGIT bispecific antibody for the treatment of solid tumors
    Authors: Y Xiao, P Chen, C Luo, Z Xu, X Li, L Liu, L Zhao
    Cancer treatment and research communications, 2021-09-27;29(0):100467.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. LFA-1 and Kindlin-3 enable the collaborative transport of SLP-76 microclusters by myosin and dynein motors
    Authors: KP Eidell, A Lovy, NR Sylvain, FA Scangarell, HI Muendlein, MJ Ophir, K Nguyen, MC Seminario, SC Bunnell
    Journal of Cell Science, 2021-08-27;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. A scDb-based trivalent bispecific antibody for T-cell-mediated killing of HER3-expressing cancer cells
    Authors: N Aschmoneit, S Steinlein, L Kühl, O Seifert, RE Kontermann
    Scientific Reports, 2021-07-06;11(1):13880.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Loureirin B Exerts its Immunosuppressive Effects by Inhibiting STIM1/Orai1 and KV1.3 Channels
    Authors: S Shi, Q Zhao, C Ke, S Long, F Zhang, X Zhang, Y Li, X Liu, H Hu, S Yin
    Frontiers in Pharmacology, 2021-06-25;12(0):685092.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Immunomodulatory mAbs as Tools to Investigate on Cis-Interaction of PD-1/PD-L1 on Tumor Cells and to Set Up Methods for Early Screening of Safe and Potent Combinatorial Treatments
    Authors: C Vetrei, M Passariell, G Froechlich, R Rapuano Le, N Zambrano, C De Lorenzo
    Cancers, 2021-06-08;13(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. A Rational Designed Novel Bispecific Antibody for the Treatment of GBM
    Authors: R Sun, Y Zhou, L Han, Z Pan, J Chen, H Zong, Y Bian, H Jiang, B Zhang, J Zhu
    Biomedicines, 2021-06-03;9(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. T cell receptor-dependent S-acylation of ZAP-70 controls activation of T cells
    Authors: R Tewari, B Shayahati, Y Fan, AM Akimzhanov
    The Journal of Biological Chemistry, 2021-01-19;0(0):100311.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Altered plasma levels of &betaC and &gammaC chain cytokines and post-treatment modulation in tuberculous lymphadenitis
    Authors: GR Kathamuthu, K Moideen, R Sridhar, D Baskaran, S Babu
    Cytokine, 2020-12-17;138(0):155405.
    Species: Human
    Sample Types: Plasma
  22. Aromadendrin Inhibits T Cell Activation via Regulation of Calcium Influx and NFAT Activity
    Authors: HS Lee, GS Jeong
    Molecules, 2020-10-08;25(19):.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Lentiviral delivery of combinatorial CAR/CRISPRi circuit into human primary T cells is enhanced by TBK1/IKK? complex inhibitor BX795
    Authors: L Li, Y Gao, R Srivastava, W Wang, Q Xiong, Z Fang, A Pelayo, C Denson, A Goswami, R Harari-Ste, Z Yang, L Weng, LS Qi, FM Marincola
    Journal of Translational Medicine, 2020-09-23;18(1):363.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Targeting PD-L1 in non-small cell lung cancer using CAR T cells
    Authors: M Liu, X Wang, W Li, X Yu, P Flores-Vil, ZY Xu-Monette, L Li, M Zhang, KH Young, X Ma, Y Li
    Oncogenesis, 2020-08-13;9(8):72.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Isolation of Two Novel Human Anti-CTLA-4 mAbs with Intriguing Biological Properties on Tumor and NK Cells
    Authors: M Passariell, C Vetrei, E Sasso, G Froechlich, C Gentile, AM D'Alise, N Zambrano, E Scarselli, A Nicosia, C De Lorenzo
    Cancers (Basel), 2020-08-06;12(8):.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. IRAP-dependent endosomal T cell receptor signalling is essential for T cell responses
    Authors: I Evnouchido, P Chappert, S Benadda, A Zucchetti, M Weimershau, M Bens, V Caillens, D Koumantou, S Lotersztaj, P van Endert, J Davoust, P Guermonpre, C Hivroz, DA Gross, L Saveanu
    Nat Commun, 2020-06-02;11(1):2779.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability
    Authors: S Guedan, A Madar, V Casado-Med, C Shaw, A Wing, F Liu, RM Young, CH June, AD Posey
    J. Clin. Invest., 2020-06-01;130(6):3087-3097.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Gaps in Study Design for Immune Parameter Research for Latent Tuberculosis Infection: A Systematic Review
    Authors: M Herrera, C Vera, Y Keynan, ZV Rueda
    J Immunol Res, 2020-04-21;2020(0):8074183.
    Species: Human
    Sample Types: Plasma
  29. Ipilimumab and Its Derived EGFR Aptamer-Based Conjugate Induce Efficient NK Cell Activation against Cancer Cells
    Authors: M Passariell, S Camorani, C Vetrei, S Ricci, L Cerchia, C De Lorenzo
    Cancers (Basel), 2020-02-01;12(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Ultrasmall silica nanoparticles directly ligate the T cell receptor complex
    Authors: B Vis, RE Hewitt, TP Monie, C Fairbairn, SD Turner, SD Kinrade, JJ Powell
    Proc. Natl. Acad. Sci. U.S.A., 2019-12-23;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Type 1 diabetic mellitus patients with increased atherosclerosis risk display decreased CDKN2A/2B/2BAS gene expression in leukocytes
    Authors: S Martínez-H, V Sánchez-Ga, A Herrero-Ce, Á Vinué, JT Real, JF Ascaso, DJ Burks, H González-N
    J Transl Med, 2019-07-12;17(1):222.
    Species: Human
    Sample Types: Plasma
  32. Quantitative Interactomics in Primary T Cells Provides a Rationale for Concomitant PD-1 and BTLA Coinhibitor Blockade in Cancer Immunotherapy
    Authors: J Celis-Guti, P Blattmann, Y Zhai, N Jarmuzynsk, K Ruminski, C Grégoire, Y Ounoughene, F Fiore, R Aebersold, R Roncagalli, M Gstaiger, B Malissen
    Cell Rep, 2019-06-11;27(11):3315-3330.e7.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Role of vimentin in modulating immune cell apoptosis and inflammatory responses in sepsis
    Authors: L Su, P Pan, P Yan, Y Long, X Zhou, X Wang, R Zhou, B Wen, L Xie, D Liu
    Sci Rep, 2019-04-05;9(1):5747.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Urinary and serum soluble CD25 complements urinary soluble CD163 to detect active renal anti-neutrophil cytoplasmic autoantibody-associated vasculitis: a cohort study
    Authors: GJ Dekkema, WH Abdulahad, T Bijma, SM Moran, L Ryan, MA Little, CA Stegeman, P Heeringa, JF Sanders
    Nephrol. Dial. Transplant., 2019-02-01;0(0):.
    Species: Human
    Sample Types: Serum
  35. Sulphamethazine derivatives as immunomodulating agents: New therapeutic strategies for inflammatory diseases
    Authors: H Siddiqui, HM Haniffa, A Jabeen, AU -Rahman, MI Choudhary
    PLoS ONE, 2018-12-19;13(12):e0208933.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. Modulation of intracellular calcium signaling by microRNA-34a-5p
    Authors: C Diener, M Hart, D Alansary, V Poth, B Walch-Rück, J Menegatti, F Grässer, T Fehlmann, S Rheinheime, BA Niemeyer, HP Lenhof, A Keller, E Meese
    Cell Death Dis, 2018-09-27;9(10):1008.
    Species: Human
    Sample Types: Whole Cells
  37. Interferon-? converts human microvascular pericytes into negative regulators of alloimmunity through induction of indoleamine 2,3-dioxygenase 1
    Authors: R Liu, J Merola, TD Manes, L Qin, GT Tietjen, F López-Girá, V Broecker, C Fang, C Xie, PM Chen, NC Kirkiles-S, D Jane-Wit, JS Pober
    JCI Insight, 2018-03-08;3(5):.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. Enhancing CAR T cell persistence through ICOS and 4-1BB costimulation.
    Authors: Guedan S, Posey A, Shaw C, Wing A, Da T, Patel P, McGettigan S, Casado-Medrano V, Kawalekar O, Uribe-Herranz M, Song D, Melenhorst J, Lacey S, Scholler J, Keith B, Young R, June C
    JCI Insight, 2018-01-11;3(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. One-domain CD4 Fused to Human Anti-CD16 Antibody Domain Mediates Effective Killing of HIV-1-Infected Cells
    Authors: W Li, Y Wu, D Kong, H Yang, Y Wang, J Shao, Y Feng, W Chen, L Ma, T Ying, DS Dimitrov
    Sci Rep, 2017-08-22;7(1):9130.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Identification of benzazole compounds that induce HIV-1 transcription
    Authors: JD Graci, D Michaels, G Chen, GM Schiralli, S Nodder, M Weetall, GM Karp, Z Gu, JM Colacino, AJ Henderson
    PLoS ONE, 2017-06-28;12(6):e0179100.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Reduced systemic and mycobacterial antigen-stimulated concentrations of IL-1? and IL-18 in tuberculous lymphadenitis
    Cytokine, 2016-10-26;90(0):66-72.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. Chemical proteomic map of dimethyl fumarate-sensitive cysteines in primary human T cells
    Authors: Benjamin F Cravatt
    Sci Signal, 2016-09-13;9(445):rs10.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  43. Rac1-Rab11-FIP3 regulatory hub coordinates vesicle traffic with actin remodeling and T-cell activation
    EMBO J, 2016-05-06;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Additive anti-inflammatory effects of corticosteroids and phosphodiesterase-4 inhibitors in COPD CD8 cells.
    Authors: Grundy S, Plumb J, Kaur M, Ray D, Singh D
    Respir Res, 2016-01-25;17(1):9.
    Species: Human
    Sample Types: Cell Culture Supernates
  45. Characterization of host and microbial determinants in individuals with latent tuberculosis infection using a human granuloma model.
    Authors: Guirado E, Mbawuike U, Keiser T, Arcos J, Azad A, Wang S, Schlesinger L
    MBio, 2015-02-17;6(1):e02537-14.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. Anti-inflammatory effects of green soybean extract irradiated with visible light.
    Authors: Tanaka K, Ohgo Y, Katayanagi Y, Yasui K, Hiramoto S, Ikemoto H, Nakata Y, Miyoshi N, Isemura M, Ohashi N, Imai S
    Sci Rep, 2014-04-22;4(0):4732.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. Engagement of SLAMF2/CD48 prolongs the time frame of effective T cell activation by supporting mature dendritic cell survival.
    Authors: Kis-Toth K, Tsokos G
    J Immunol, 2014-03-26;192(9):4436-42.
    Species: Human
    Sample Types: Cell Culture Supernates
  48. The aryl hydrocarbon receptor is functionally upregulated early in the course ofvhuman T-cell activation.
    Authors: Prigent L, Robineau M, Jouneau S, Morzadec C, Louarn L, Vernhet L, Fardel O, Sparfel L
    Eur J Immunol, 2014-02-18;44(5):1330-40.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. The tuberculosis vaccine candidate Bacillus Calmette-Guerin DeltaureC::hly coexpressing human interleukin-7 or -18 enhances antigen-specific T cell responses in mice.
    Authors: Rao M, Vogelzang A, Kaiser P, Schuerer S, Kaufmann S, Gengenbacher M
    PLoS ONE, 2013-11-13;8(11):e78966.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. T-cell engager-armed oncolytic vaccinia virus significantly enhances antitumor therapy.
    Authors: Yu F, Wang X, Guo Z, Bartlett D, Gottschalk S, Song X
    Mol Ther, 2013-10-17;22(1):102-11.
    Species: Human
    Sample Types: Cell Culture Supernates
  51. Dynamic motile T cells highly respond to the T cell stimulation via PI3K-Akt and NF-kappaB pathways.
    Authors: Kim H, Na B, Kwon M, Ko Y, Han W, Jun C
    PLoS ONE, 2013-03-26;8(3):e59793.
    Species: Human
    Sample Types: Cell Culture Supernates
  52. Leukocyte function-associated antigen-1/intercellular adhesion molecule-1 interaction induces a novel genetic signature resulting in T-cells refractory to transforming growth factor-beta signaling.
    Authors: Verma N, Dempsey E, Long A, Davies A, Barry S, Fallon P, Volkov Y, Kelleher D
    J Biol Chem, 2012-06-15;287(32):27204-16.
    Species: Human
    Sample Types: Cell Culture Supernates
  53. Immunoregulation of autocrine prolactin: suppressing the expression of costimulatory molecules and cytokines in T lymphocytes by prolactin receptor knockdown.
    Authors: Xu D, Lin L, Lin X
    Cell. Immunol., 2010-03-02;263(1):71-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  54. IL-8 dictates glycosaminoglycan binding and stability of IL-18 in cystic fibrosis.
    Authors: Reeves EP, Williamson M, Byrne B, Bergin DA, Smith SG, Greally P, O'Kennedy R, O'Neill SJ, McElvaney NG
    J. Immunol., 2009-12-21;184(3):1642-52.
    Species: Human
    Sample Types: Serum
  55. Fungal proteases induce Th2 polarization through limited dendritic cell maturation and reduced production of IL-12.
    Authors: Lamhamedi-Cherradi SE, Martin RE, Ito T, Kheradmand F, Corry DB, Liu YJ, Moyle M
    J. Immunol., 2008-05-01;180(9):6000-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  56. Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling.
    Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M
    J. Clin. Invest., 2007-12-01;117(12):3664-3672.
    Species: Human
    Sample Types: Cell Culture Supernates
  57. Calcium dependence of T cell proliferation following focal stimulation.
    Authors: Schwarz EC, Kummerow C, Wenning AS, Wagner K, Sappok A, Waggershauser K, Griesemer D, Strauss B, Wolfs MJ, Quintana A, Hoth M
    Eur. J. Immunol., 2007-10-01;37(10):2723-33.
    Species: Human
    Sample Types: Cell Culture Supernates
  58. Immunotherapy for neuroblastoma using syngeneic fibroblasts transfected with IL-2 and IL-12.
    Authors: Barker SE, Grosse SM, Siapati EK, Kritz A, Kinnon C, Thrasher AJ, Hart SL
    Br. J. Cancer, 2007-06-26;97(2):210-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  59. Familial NK cell deficiency associated with impaired IL-2- and IL-15-dependent survival of lymphocytes.
    Authors: Eidenschenk C, Jouanguy E, Alcais A, Mention JJ, Pasquier B, Fleckenstein IM, Puel A, Gineau L, Carel JC, Vivier E, Le Deist F, Casanova JL
    J. Immunol., 2006-12-15;177(12):8835-43.
    Species: Human
    Sample Types: Cell Culture Supernates
  60. Splice variants of human FOXP3 are functional inhibitors of human CD4+ T-cell activation.
    Authors: Smith EL, Finney HM, Nesbitt AM, Ramsdell F, Robinson MK
    Immunology, 2006-10-01;119(2):203-11.
    Species: Human
    Sample Types: Cell Culture Supernates
  61. The Wingless homolog WNT5A and its receptor Frizzled-5 regulate inflammatory responses of human mononuclear cells induced by microbial stimulation.
    Authors: Blumenthal A, Ehlers S, Lauber J, Buer J, Lange C, Goldmann T, Heine H, Brandt E, Reiling N
    Blood, 2006-04-06;108(3):965-73.
    Species: Human
    Sample Types: Cell Culture Supernates
  62. Human T cells constitutively express IL-15 that promotes ex vivo T cell homeostatic proliferation through autocrine/juxtacrine loops.
    Authors: Miranda-Carus ME, Benito-Miguel M, Llamas MA, Balsa A, Martin-Mola E
    J. Immunol., 2005-09-15;175(6):3656-62.
    Species: Human
    Sample Types: Cell Culture Supernates
  63. Manipulation of host cytokine network by ticks: a potential gateway for pathogen transmission.
    Authors: Hajnicka V, Vancova I, Kocakova P, Slovak M, Gasperik J, Slavikova M, Hails RS, Labuda M, Nuttall PA
    Parasitology, 2005-03-01;130(0):333-42.
    Species: Human
    Sample Types: Saliva
  64. Immune responses in normal Indian langur monkeys (Presbytis entellus)--a primate model for visceral leishmaniasis.
    Authors: Misra A, Dube A, Naik S
    J. Med. Primatol., 2004-04-01;33(2):65-9.
    Species: Primate - Presbytis entellus (Indian langur)
    Sample Types: Cell Culture Supernates
  65. Activation of resting human primary T cells with chimeric receptors: costimulation from CD28, inducible costimulator, CD134, and CD137 in series with signals from the TCR zeta chain.
    Authors: Finney HM, Akbar AN, Lawson AD
    J. Immunol., 2004-01-01;172(1):104-13.
    Species: Human
    Sample Types: Cell Culture Supernates
  66. Mycophenolic acid inhibits IL-2-dependent T cell proliferation, but not IL-2-dependent survival and sensitization to apoptosis.
    Authors: Quemeneur L, Flacher M, Gerland LM, Ffrench M, Revillard JP, Bonnefoy-Berard N
    J. Immunol., 2002-09-01;169(5):2747-55.
    Species: Human
    Sample Types: Cell Culture Supernates

FAQs

  1. Is PBS a suitable alternative with which to formulate the Reagent Diluent?

    • Our lab has determined that Tris-buffered saline generates optimal assay performance in comparison to PBS for this assay. This assay is validated with Reagent Diluent formulated using Tris-buffered saline.

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Reviews for Human IL-2 DuoSet ELISA

Average Rating: 4.5 (Based on 12 Reviews)

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Human IL-2 DuoSet ELISA
By Anonymous on 02/27/2023
Sample Tested: Cell culture supernatant

Human IL-2 DuoSet ELISA
By Anonymous on 01/27/2021
Sample Tested: Cell culture supernatant

Human IL-2 DuoSet ELISA
By Robert Berahovich on 09/29/2020
Sample Tested: Tissue Culture Media

Detection of IL-2 produced by BCMA-specific CAR-T cells in response to BCMA-negative and BCMA-positive hematopoietic cell lines.


Human IL-2 DuoSet ELISA
By Anonymous on 09/26/2020
Sample Tested: Serum

Human IL-2 DuoSet ELISA
By Liang Lin on 08/26/2020
Sample Tested: Activated T cell

Human IL-2 DuoSet ELISA
By Anonymous on 07/31/2020
Sample Tested: Cell culture supernatant

We used this kit for the quantification of IL-2 in the cell culture supernatant of human CAR T cells. The protocol is well described and the standard curve works well.


Human IL-2 DuoSet ELISA
By Anonymous on 04/02/2020
Sample Tested: Serum and Plasma

We used this kit for the quantification of Il-2 in human serum and plasma. We could not detect Il-2 in our samples, but the standard curve works very well and well-described protocol.


Human IL-2 DuoSet ELISA
By Anonymous on 12/11/2019
Sample Tested: human primary T cells

Human IL-2 DuoSet ELISA
By Anonymous on 05/06/2019
Sample Tested: Cell culture supernatant

Human IL-2 DuoSet ELISA
By Anonymous on 10/12/2018
Sample Tested: Cell culture supernatant

Human IL-2 DuoSet ELISA
By Anonymous on 08/06/2018
Sample Tested: Serum

Human IL-2 DuoSet ELISA
By Shelley Anderson on 10/20/2017
Sample Tested: Human cells