• Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)
  • Sensitivity
    7 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human IL-2
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC01-1, Quantikine Immunoassay Control Group 1 - Please Inquire
Product Summary
The Quantikine Human IL-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-2 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-2 and antibodies raised against recombinant human IL-2. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-2 showed dose response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-2

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean67.54388512326201148
Standard Deviation2.914.426.923.234.445.5
CV%4.33.33.2105.54

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean9861911832507551470
Standard Deviation4.2182412.627.874.1
CV%4.32.9253.75

Recovery

The recovery of human IL-2 spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 101 96-107
Citrate Plasma (n=5) 105 98-111
EDTA Plasma (n=5) 118 104-139
Heparin Plasma (n=5) 109 94-129
Serum (n=5) 99 89-114
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of IL-2 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-2 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-2
IL-2 (Interleukin 2) is a critical cytokine in T cell biology. It promotes T cell activation and expansion, the development, maintenance and function of regulatory T cells (Treg), and the differentiation of CD8+ T cells into terminal effector cells and memory cells. IL-2 signals through a receptor complex composed of CD25/IL-2 R alpha, IL-2 R beta, and the Common gamma Chain (gamma c). IL-2 R beta is also a component of the IL-15 receptor complex. Gamma c is also a signaling subunit in the receptors for IL-4, -7, -9, -15, and -21.
    • Long Name
      Interleukin 2
    • Entrez Gene IDs
      3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit);
    • Alternate Names
      aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 3 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    48 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Diminished plasma levels of common ?-chain cytokines in pulmonary tuberculosis and reversal following treatment
      Authors: NP Kumar, VV Banurekha, D Nair, S Babu
      PLoS ONE, 2017;12(4):e0176495.
      Species: Human
      Sample Type: Plasma
    2. T Cell Subset and Stimulation Strength-Dependent Modulation of T Cell Activation by Kv1.3 Blockers
      Authors: WP Fung-Leung, W Edwards, Y Liu, K Ngo, J Angsana, G Castro, N Wu, X Liu, RV Swanson, AD Wickenden
      PLoS ONE, 2017;12(1):e0170102.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. The Suppressed Induction of Human Mature Cytotoxic T Lymphocytes Caused by Asbestos Is Not due to Interleukin-2 Insufficiency
      J Immunol Res, 2016;2016(0):7484872.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. Involvement of NK Cells and NKp30 Pathway in Antisynthetase Syndrome
      J Immunol, 2016;197(5):1621-30.
      Species: Human
      Sample Type: Serum
    5. Inhibition of G-protein betagamma signaling enhances T cell receptor-stimulated interleukin 2 transcription in CD4+ T helper cells.
      Authors: Yost E, Hynes T, Hartle C, Ott B, Berlot C
      PLoS ONE, 2015;10(1):e0116575.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. The injury-induced myokine insulin-like 6 is protective in experimental autoimmune myositis.
      Authors: Zeng L, Maruyama S, Nakamura K, Parker-Duffen J, Adham I, Zhong X, Lee H, Querfurth H, Walsh K
      Skelet Muscle, 2015;4(0):16.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. Impact of Exogenous Galectin-9 on Human T Cells: CONTRIBUTION OF THE T CELL RECEPTOR COMPLEX TO ANTIGEN-INDEPENDENT ACTIVATION BUT NOT TO APOPTOSIS INDUCTION.
      Authors: Lhuillier C, Barjon C, Niki T, Gelin A, Praz F, Morales O, Souquere S, Hirashima M, Wei M, Dellis O, Busson P
      J Biol Chem, 2015;290(27):16797-811.
      Species: Human
      Sample Type: Cell Culture Supernates
    8. The Association between Serum Cytokines and Damage to Large and Small Nerve Fibers in Diabetic Peripheral Neuropathy.
      Authors: Magrinelli F, Briani C, Romano M, Ruggero S, Toffanin E, Triolo G, Peter G, Praitano M, Lauriola M, Zanette G, Tamburin S
      J Diabetes Res, 2015;2015(0):547834.
      Species: Human
      Sample Type: Serum
    9. Sos1 regulates sustained TCR-mediated Erk activation.
      Authors: Poltorak M, Meinert I, Stone J, Schraven B, Simeoni L
      Eur J Immunol, 2014;44(5):1535-40.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. Targeting the ion channel Kv1.3 with scorpion venom peptides engineered for potency, selectivity, and half-life.
      Authors: Edwards W, Fung-Leung W, Huang C, Chi E, Wu N, Liu Y, Maher M, Bonesteel R, Connor J, Fellows R, Garcia E, Lee J, Lu L, Ngo K, Scott B, Zhou H, Swanson R, Wickenden A
      J Biol Chem, 2014;289(33):22704-14.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. Activation of silenced cytokine gene promoters by the synergistic effect of TBP-TALE and VP64-TALE activators.
      Authors: Anthony, Kim, More, Abhijit, Zhang, Xiaoliu
      PLoS ONE, 2014;9(4):e95790.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Role of programmed death ligands in effective T-cell interactions in extranodal natural killer/T-cell lymphoma.
      Authors: Han L, Liu F, Li R, Li Z, Chen X, Zhou Z, Zhang X, Hu T, Zhang Y, Young K, Sun S, Wen J, Zhang M
      Oncol Lett, 2014;8(4):1461-1469.
      Species: Human
      Sample Type: Serum
    13. Immunogenicity of a vaccine regimen composed of simian immunodeficiency virus DNA, rMVA, and viral particles administered to female rhesus macaques via four different mucosal routes.
      Authors: Manrique M, Kozlowski P, Cobo-Molinos A, Wang S, Wilson R, Montefiori D, Carville A, Aldovini A
      J Virol, 2013;87(8):4738-50.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Multiplexing immunoassays for cytokine detection in the serum of patients with rheumatoid arthritis: lack of sensitivity and interference by rheumatoid factor.
      Authors: Churchman SM, Geiler J, Parmar R, Horner EA, Church LD, Emery P, Buch MH, McDermott MF, Ponchel F
      Clin. Exp. Rheumatol., 2012;30(4):534-42.
      Species: Human
      Sample Type: Serum
    15. Origination of new immunological functions in the costimulatory molecule B7-H3: the role of exon duplication in evolution of the immune system.
      Authors: Sun J, Fu F, Gu W, Yan R, Zhang G, Shen Z, Zhou Y, Wang H, Shen B, Zhang X
      PLoS ONE, 2011;6(9):e24751.
      Species: Human
      Sample Type: Cell Culture Supernates
    16. HDAC inhibition by LBH589 affects the phenotype and function of human myeloid dendritic cells.
      Authors: Song W, Tai YT, Tian Z, Hideshima T, Chauhan D, Nanjappa P, Exley MA, Anderson KC, Munshi NC
      Leukemia, 2011;25(1):161-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    17. Antigen presentation and MHC class II expression by human esophageal epithelial cells: role in eosinophilic esophagitis.
      Authors: Mulder DJ, Pooni A, Mak N, Hurlbut DJ, Basta S, Justinich CJ
      Am. J. Pathol., 2011;178(2):744-53.
      Species: Human
      Sample Type: Cell Culture Supernates
    18. Immunologic markers as predictors of tuberculosis-associated immune reconstitution inflammatory syndrome in HIV and tuberculosis coinfected persons in Thailand.
      Authors: Tieu HV, Ananworanich J, Avihingsanon A, Apateerapong W, Sirivichayakul S, Siangphoe U, Klongugkara S, Boonchokchai B, Hammer SM, Manosuthi W
      AIDS Res. Hum. Retroviruses, 2009;25(11):1083-9.
      Species: Human
      Sample Type: Serum
    19. Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
      Authors: Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G
      Transplantation, 2009;88(9):1109-16.
      Species: Human
      Sample Type: Urine
    20. CD200 is induced by ERK and is a potential therapeutic target in melanoma.
      Authors: Petermann KB, Rozenberg GI, Zedek D, Groben P, McKinnon K, Buehler C, Kim WY, Shields JM, Penland S, Bear JE, Thomas NE, Serody JS, Sharpless NE
      J. Clin. Invest., 2007;117(12):3922-3929.
      Species: Human
      Sample Type: Cell Culture Supernates
    21. Short communication: decreasing soluble CD30 and increasing IFN-gamma plasma levels are indicators of effective highly active antiretroviral therapy.
      Authors: Sadeghi M, Susal C, Daniel V, Naujokat C, Zimmermann R, Huth-Kuhne A, Opelz G
      AIDS Res. Hum. Retroviruses, 2007;23(7):886-90.
      Species: Human
      Sample Type: Plasma
    22. Co-occurrence of IgA antibodies against ethanol metabolites and tissue transglutaminase in alcohol consumers: correlation with proinflammatory cytokines and markers of fibrogenesis.
      Authors: Koivisto H, Hietala J, Anttila P, Niemela O
      Dig. Dis. Sci., 2007;53(2):500-5.
      Species: Human
      Sample Type: Serum
    23. 1, 25 Dihydroxyvitamin D3 modulated cytokine response in pulmonary tuberculosis.
      Authors: Vidyarani M, Selvaraj P, Jawahar MS, Narayanan PR
      Cytokine, 2007;40(2):128-34.
      Species: Human
      Sample Type: Cell Culture Supernates
    24. Functional polymorphisms in FAS and FASL contribute to increased apoptosis of tumor infiltration lymphocytes and risk of breast cancer.
      Authors: Zhang B, Sun T, Xue L, Han X, Zhang B, Lu N, Shi Y, Tan W, Zhou Y, Zhao D, Zhang X, Guo Y, Lin D
      Carcinogenesis, 2007;28(5):1067-73.
      Species: Human
      Sample Type: Cell Culture Supernates
    25. Expression of B7-H1 on gastric epithelial cells: its potential role in regulating T cells during Helicobacter pylori infection.
      Authors: Das S, Suarez G, Beswick EJ, Sierra JC, Graham DY, Reyes VE
      J. Immunol., 2006;176(5):3000-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    26. Decreased plasma CXCL16/SR-PSOX concentration is associated with coronary artery disease.
      Authors: Sheikine Y, Bang CS, Nilsson L, Samnegard A, Hamsten A, Jonasson L, Eriksson P, Sirsjo A
      Atherosclerosis, 2006;188(2):462-6.
      Species: Human
      Sample Type: Serum
    27. Influence of eotaxin 67G>A polymorphism on plasma eotaxin concentrations in myocardial infarction survivors and healthy controls.
      Authors: Sheikine Y, Olsen B, Gharizadeh B, Jatta K, Tornvall P, Ghaderi M
      Atherosclerosis, 2006;189(2):458-63.
      Species: Human
      Sample Type: Serum
    28. Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
      Authors: Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S
      Gut, 2006;56(2):243-52.
      Species: Human
      Sample Type: Cell Culture Supernates
    29. Interleukin-2 in human milk: a potential modulator of lymphocyte development in the breastfed infant.
      Authors: Bryan DL, Forsyth KD, Gibson RA, Hawkes JS
      Cytokine, 2006;33(5):289-93.
      Species: Human
      Sample Type: Milk
    30. C-reactive protein and vascular cell adhesion molecule-1 as markers of severity in sickle cell disease.
      Authors: Makis AC, Hatzimichael EC, Stebbing J, Bourantas KL
      Arch. Intern. Med., 2006;166(3):366-8.
      Species: Human
      Sample Type: Plasma
    31. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
      Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
      Microbes Infect., 2005;7(3):427-36.
      Species: Human
      Sample Type: Serum
    32. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
      Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
      Microbes Infect., 2005;7(3):427-36.
      Species: Human
      Sample Type: Serum
    33. Strong inflammatory cytokine response in male and strong anti-inflammatory response in female kidney transplant recipients with urinary tract infection.
      Authors: Sadeghi M, Daniel V, Naujokat C, Wiesel M, Hergesell O, Opelz G
      Transpl. Int., 2005;18(2):177-85.
      Species: Human
      Sample Type: Plasma
    34. Primary cutaneous T-cell lymphomas show a deletion or translocation affecting NAV3, the human UNC-53 homologue.
      Authors: Karenko L, Hahtola S, Paivinen S, Karhu R, Syrja S, Kahkonen M, Nedoszytko B, Kytölä S, Zhou Y, Blazevic V, Pesonen M, Nevala H, Nupponen N, Sihto H, Krebs I, Poustka A, Roszkiewicz J, Saksela K, Peterson P, Visakorpi T, Ranki A
      Cancer Res., 2005;65(18):8101-10.
      Species: Human
      Sample Type: Cell Culture Supernates
    35. Cytokines and adhesion molecules in multiple sclerosis patients treated with interferon-beta1b.
      Authors: Jensen J, Krakauer M, Sellebjerg F
      Cytokine, 2005;29(1):24-30.
      Species: Human
      Sample Type: Plasma
    36. Inflammatory cytokine concentrations are elevated in seminal plasma of men with spinal cord injuries.
      Authors: Basu S, Aballa TC, Ferrell SM, Lynne CM, Brackett NL
      J. Androl., 2004;25(2):250-4.
      Species: Human
      Sample Type: Seminal Plasma
    37. Urinary IL-2 assay for monitoring intravesical bacillus Calmette-Guerin response of superficial bladder cancer during induction course and maintenance therapy.
      Authors: Saint F, Kurth N, Maille P, Vordos D, Hoznek A, Soyeux P, Patard JJ, Abbou CC, Chopin DK
      Int. J. Cancer, 2003;107(3):434-40.
      Species: Human
      Sample Type: Urine
    38. Local immunostimulation induced by intravesical administration of autologous interferon-gamma-activated macrophages in patients with superficial bladder cancer.
      Authors: Pages F, Lebel-Binay S, Vieillefond A, Deneux L, Cambillau M, Soubrane O, Debre B, Tardy D, Lemonne JL, Abastado JP, Fridman WH, Thiounn N
      Clin. Exp. Immunol., 2002;127(2):303-9.
      Species: Human
      Sample Type: Urine
    39. Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15.
      Authors: Briard D, Brouty-Boye D, Azzarone B, Jasmin C
      J. Immunol., 2002;168(9):4326-32.
      Species: Human
      Sample Type: Cell Culture Supernates
    40. Embryotoxicity of peritoneal fluid in women with endometriosis. Its relation with cytokines and lymphocyte populations.
      Authors: Gomez-Torres MJ, Acien P, Campos A, Velasco I
      Hum. Reprod., 2002;17(3):777-81.
      Species: Human
      Sample Type: Peritoneal Fluid
    41. Intraperitoneal bispecific antibody (HEA125xOKT3) therapy inhibits malignant ascites production in advanced ovarian carcinoma.
      Authors: Marme A, Strauss G, Bastert G, Grischke EM, Moldenhauer G
      Int. J. Cancer, 2002;101(2):183-9.
      Species: Human
      Sample Type: Ascites Fluid
    42. Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor.
      Authors: Bovolenta C, Pilotti E, Mauri M, Turci M, Ciancianaini P, Fisicaro P, Bertazzoni U, Poli G, Casoli C
      Blood, 2002;99(1):224-31.
      Species: Human
      Sample Type: Cell Culture Supernates
    43. An analysis of acute changes in interleukin-6 levels after treatment of hepatitis C with consensus interferon.
      Authors: Cotler SJ, Reddy KR, McCone J, Wolfe DL, Liu A, Craft TR, Ferris MW, Conrad AJ, Albrecht J, Morrissey M, Ganger DR, Rosenblate H, Blatt LM, Jensen DM, Taylor MW
      J. Interferon Cytokine Res., 2001;21(12):1011-9.
      Species: Human
      Sample Type: Serum
    44. High versus low basal cortisol secretion in asymptomatic, medication-free HIV-infected men: differential effects of severe life stress on parameters of immune status.
      Authors: Petitto JM, Leserman J, Perkins DO, Stern RA, Silva SG, Gettes D, Zheng B, Folds JD, Golden RN, Evans DL
      Behav Med, 2000;25(4):143-51.
      Species: Human
      Sample Type: Cell Culture Supernates
    45. Human amniotic fluid lacks interleukin-2 and interleukin-15 but can interact with the beta-chain of the interleukin-2 receptor.
      Authors: Searle RF, Bromage SJ, Palmer J, Curry JE, Lang AK
      Immunology, 2000;99(3):411-7.
      Species: Human
      Sample Type: Amniotic Fluid
    46. Effects of UVB radiation on cytokine generation, cell adhesion molecules, and cell activation markers in T-lymphocytes and peripheral blood HPCs.
      Authors: Lankford KV, Mosunjac M, Hillyer CD
      Transfusion, 2000;40(3):361-7.
      Species: Human
      Sample Type: Cell Culture Supernates
    47. Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
      Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
      J. Exp. Med., 2000;192(2):219-26.
      Species: Human
      Sample Type: Cell Culture Supernates
    48. Cytokine profiles in parotid saliva from HIV-1-infected individuals: changes associated with opportunistic infections in the oral cavity.
      Authors: Black, K P, Merrill, K W, Jackson, S, Katz, J
      Oral Microbiol Immunol, 2000;15(2):74-81.
      Species: Human
      Sample Type: Saliva
    Expand to show all 48 Citations
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     Human IL-2 Quantikine ELISA Kit D2050
    : Human IL-2 Quantikine ELISA Kit [D2050]

    Summary

    Sample TestedMouse serum

    Other Experimental Details

    Other Experimental DetailsUsed mice treated with human IL-2 product to test the IL-2 clearance in the blood.Were able to use our own IL-2 construct to make a trustable standard curve and comparable with the kit's products.We use this kit daily.

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