Human IL-2 Quantikine ELISA Kit

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Human IL-2 ELISA Standard Curve for Cell Culture Supernate Assay
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Citations (87)
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Human IL-2 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)
Sensitivity
7 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human TNF- alpha.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The QuantiGlo® Human TNF- alpha Immunoassay is a 5 hour solid phase chemiluminescent ELISA designed to measure human TNF-alpha in cell culture supernates, serum, and plasma. It contains E.coli-derived recombinant human TNF-alpha and antibodies raised against the recombinant
factor. It has been shown to accurately quantitate recombinant human TNF-alpha. Results obtained using naturally occurring TNF-alpha samples showed linear curves that were parallel to the standard curves obtained using the QuantiGlo® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TNF-alpha.

Recovery

The recovery of human TNF-alpha spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 101 96-107
Citrate Plasma (n=5) 105 98-111
EDTA Plasma (n=5) 118 104-139
Heparin Plasma (n=5) 109 94-129
Serum (n=5) 99 89-114

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human TNF- alpha in various matrices were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human IL-2 ELISA Linearity

Scientific Data

Human IL-2 ELISA Standard Curve for Cell Culture Supernate Assay

Human IL-2 ELISA Standard Curve for Serum/Plasma Assay

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-2

Interleukin 2 (IL-2), also known as T cell growth factor (TCGF), is a 15-18 kDa variably glycosylated alpha -helical polypeptide that is a member of the Common gamma Chain ( gamma c) cytokine family (1-4). It exists as a monomer and has a notably short half-life (< 30 minutes) (1). Human IL-2 is synthesized as a 153 amino acid (aa) precursor that contains a 20 aa signal sequence plus a 133 aa mature region (5, 6). The mature region is alpha -helical in nature, and contains one utilized O-linked glycosylation site at Thr3 plus three cysteines, two of which form an intrachain disulfide bond that is essential for activity (7). Mature human IL-2 shares 73%, 66%, 78% and 97% aa identity with canine, rat, feline and rhesus monkey IL-2, respectively. Although human IL-2 shares only approximately 60% aa identity with the highly polymorphic mouse IL-2, human IL-2 is known to be active on mouse IL-2 responsive cells. Cells reported to secrete IL-2 include gamma δ T cells (8), activated conventional CD4+ and CD8+ T cells (1, 9), neurons (10, 11), microglia (12), and hematopoietic stem cells (13). 

The receptor for IL-2 (IL-2 R) is composed of three subunits, the 55 kDa CD25/IL-2 R alpha chain, the 70 kDa IL-2 R beta chain, and the 65 kDa Common gamma Chain (1, 3). IL-2 first binds to CD25, the binary complex then recruits IL-2 R beta and gamma c to form the quaternary signaling complex (1, 14). In addition to IL-2, IL-2 R beta is used by IL-15 in its quaternary signaling complex. gamma c also serves as a signaling receptor for IL-4, -7, -9, -15, and -21 (1, 3). 
In vitro studies have shown an important role for IL-2 in T cell activation and expansion. In vivo, IL-2 is critical for the development, maintenance and function of regulatory T cells (Treg) which provide protection against autoimmune disease. On the other hand, IL-2 can also promote autoimmune inflammation in target organs through its roles in regulating the expression of T cell trafficking genes, and production of Th2 cytokines. Within the CD8+ T cell subset, IL-2 is essential for optimal primary responses and differentiation into terminal effector cells. IL-2 also promotes the development of activated CD8+ T cells into memory cells. (1).

Long Name:
Interleukin 2
Entrez Gene IDs:
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names:
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 3 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human IL-2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

87 Citations: Showing 1 - 10
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  1. In the Search for Biomarkers of Pulmonary Arterial Hypertension, Are Cytokines IL-2, IL-4, IL-6, IL-10, and IFN-Gamma the Right Indicators to Use?
    Authors: Tomaszewski, M;Mertowska, P;Janczewska, M;Stycze?, A;Mertowski, S;Jonas, K;Grywalska, E;Kope?, G;
    International journal of molecular sciences
    Species: Human
    Sample Types: Plasma
  2. Possible Correlation between Urocortin 1 (Ucn1) and Immune Parameters in Patients with Endometriosis
    Authors: Abramiuk, M;Frankowska, K;Ku?ak, K;Tarkowski, R;Mertowska, P;Mertowski, S;Grywalska, E;
    International journal of molecular sciences
    Species: Human
    Sample Types: Serum
  3. Novel scFv against Notch Ligand JAG1 Suitable for Development of Cell Therapies toward JAG1-Positive Tumors
    Authors: G Silva, AF Rodrigues, S Ferreira, C Matos, RP Eleutério, G Marques, K Kucheryava, AR Lemos, PMF Sousa, R Castro, A Barbas, D Simão, PM Alves
    Biomolecules, 2023-03-02;13(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. MET-induced CD73 restrains STING-mediated immunogenicity of EGFR-mutant lung cancer
    Authors: R Yoshida, M Saigi, T Tani, BF Springer, H Shibata, S Kitajima, NR Mahadevan, M Campisi, W Kim, Y Kobayashi, TC Thai, K Haratani, Y Yamamoto, SK Sundararam, EH Knelson, A Vajdi, I Canadas, R Uppaluri, CP Paweletz, JJ Miret, PH Lizotte, PC Gokhale, PA Janne, DA Barbie
    Cancer Research, 2022-11-02;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Development of Highly Effective Anti-Mesothelin hYP218 Chimeric Antigen Receptor T Cells With Increased Tumor Infiltration and Persistence for Treating Solid Tumors
    Authors: S Tomar, J Zhang, M Khanal, J Hong, A Venugopala, Q Jiang, M Sengupta, M Miettinen, N Li, I Pastan, M Ho, R Hassan
    Molecular Cancer Therapeutics, 2022-07-05;21(7):1195-1206.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. Histone Methyltransferase SETDB1 Promotes Immune Evasion in Colorectal Cancer via FOSB-Mediated Downregulation of MicroRNA-22 through BATF3/PD-L1 Pathway
    Authors: J Tian, W Wang, J Zhu, Y Zhuang, C Qi, Z Cai, W Yan, W Lu, A Shang
    Journal of Immunology Research, 2022-04-20;2022(0):4012920.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Switchable assembly and function of antibody complexes in�vivo using a small molecule
    Authors: AJ Martinko, EF Simonds, S Prasad, A Ponce, CJ Bracken, J Wei, YH Wang, TL Chow, Z Huang, MJ Evans, JA Wells, ZB Hill
    Proceedings of the National Academy of Sciences of the United States of America, 2022-03-01;119(9):.
    Species: Human
    Sample Types: Plasma
  8. Humoral Immunity to Allogeneic Immunoproteasome-Expressing Mesenchymal Stromal Cells Requires Efferocytosis by Endogenous Phagocytes
    Authors: JP Bikorimana, J Abusarah, N Salame, N El-Hachem, R Shammaa, M Rafei
    Cells, 2022-02-09;11(4):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. LncRNA NEAT1 is upregulated in recurrent aphthous stomatitis (RAS) and has predictive values
    Authors: Y Han, L Wang, Q Li, H Chen, X Ma
    BMC Oral Health, 2021-12-31;21(1):673.
    Species: Human
    Sample Types: Plasma
  10. Phenotypic characterization of NKT-like cells and evaluation of specifically related cytokines for the prediction of unexplained recurrent miscarriage
    Authors: WS Khalaf, MRA Mahmoud, WF Elkhatib, HR Hashem, WE Soliman
    Heliyon, 2021-11-16;7(11):e08409.
    Species: Human
    Sample Types: Serum
  11. Impaired Innate Immunity in Pediatric Patients Type 1 Diabetes-Focus on Toll-like Receptors Expression
    Authors: K Kurianowic, M Klatka, A Polak, A Hymos, D B?bnowska, M Podgajna, R Hrynkiewic, O Sierawska, P Nied?wiedz
    International Journal of Molecular Sciences, 2021-11-09;22(22):.
    Species: Human
    Sample Types: Plasma
  12. Topical Delivery of Rapamycin by Means of Microenvironment-Sensitive Core-Multi-Shell Nanocarriers: Assessment of Anti-Inflammatory Activity in an ex vivo Skin/T Cell Co-Culture Model
    Authors: F Rancan, X Guo, K Rajes, P Sidiropoul, F Zabihi, L Hoffmann, S Hadam, U Blume-Peyt, E Rühl, R Haag, A Vogt
    International Journal of Nanomedicine, 2021-10-22;16(0):7137-7151.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Paracrine activity of adipose derived stem cells on limbal epithelial stem cells
    Authors: B Sikora, A Skubis-Sik, A Prusek, J Gola
    Scientific Reports, 2021-10-07;11(1):19956.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Determination of phenolic profiles of Herniaria polygama and Herniaria incana fractions and their in vitro antioxidant and anti-inflammatory effects
    Authors: J Kolodziejc, S Kozachok, ? Pecio, S Marchyshyn, W Oleszek
    Phytochemistry, 2021-07-27;190(0):112861.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Effects of Ingesting Fucoidan Derived from Cladosiphon okamuranus Tokida on Human NK Cells: A Randomized, Double-Blind, Parallel-Group, Placebo-Controlled Pilot Study
    Authors: M Tomori, T Nagamine, T Miyamoto, M Iha
    Marine Drugs, 2021-06-15;19(6):.
    Species: Human
    Sample Types: Plasma
  16. Pomalidomide restores immune recognition of primary effusion lymphoma through upregulation of ICAM-1 and B7-2
    Authors: P Shrestha, DA Davis, HK Jaeger, A Stream, AI Aisabor, R Yarchoan
    PloS Pathogens, 2021-01-07;17(1):e1009091.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Synthesis and Characterization of Store-Operated Calcium Entry Inhibitors Active in the Submicromolar Range
    Authors: C Le Guilche, T Luyten, JB Parys, M Pucheault, O Dellis
    International Journal of Molecular Sciences, 2020-12-21;21(24):.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Aberrant recruitment of leukocytes defines poor wound healing in patients with recessive dystrophic epidermolysis bullosa
    Authors: T Phillips, L Huitema, R Cepeda, DL Cobos, RIM Perez, MS Garza, F Ringpfeil, B Dasgeb, J Uitto, JC Salas-Alan, V Alexeev, O Igoucheva
    J Dermatol Sci, 2020-10-17;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. 'Psoriasis 1' reduces T‑lymphocyte‑mediated inflammation in patients with psoriasis by inhibiting vitamin D receptor‑mediated STAT4 inactivation.
    Authors: Gao Y, Sun W, Cha X, Wang H
    Int J Mol Med, 2020-08-06;46(4):1538-1550.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Identification of Novel Molecular Markers of Human Th17 Cells
    Authors: A Sa?kowska, K Kara?, I Karwaciak, A Walczak-Dr, M Krawczyk, M Sobalska-K, J Dastych, M Ratajewski
    Cells, 2020-07-03;9(7):.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Overexpression of PD-1 on Peripheral Blood Lymphocytes in Patients with Idiopathic Pulmonary Arterial Hypertension and Its Association with High Viral Loads of Epstein-Barr Virus and Poor Clinical Parameters
    Authors: M Tomaszewsk, E Grywalska, A Tomaszewsk, P B?aszczak, M Kurzyna, J Roli?ski, G Kope?
    J Clin Med, 2020-06-24;9(6):.
    Species: Human
    Sample Types: Plasma
  22. Gaps in Study Design for Immune Parameter Research for Latent Tuberculosis Infection: A Systematic Review
    Authors: M Herrera, C Vera, Y Keynan, ZV Rueda
    J Immunol Res, 2020-04-21;2020(0):8074183.
    Species: Human
    Sample Types: Plasma
  23. CCL11 increases the proportion of CD4+CD25+Foxp3+ Treg cells and the production of IL?2 and TGF?&beta by CD4+ T�cells via the STAT5 signaling pathway
    Authors: R Wang, K Huang
    Mol Med Rep, 2020-04-01;0(0):.
    Species: Human
    Sample Types: Serum
  24. MUC1 as a target for CAR-T therapy in head and neck squamous cell carinoma
    Authors: Z Mei, K Zhang, AK Lam, J Huang, F Qiu, B Qiao, Y Zhang
    Cancer Med, 2019-12-04;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. 4D electron microscopy of T cell activation
    Authors: Y Lu, BK Yoo, AHC Ng, J Kim, S Yeom, J Tang, MM Lin, AH Zewail, JR Heath
    Proc. Natl. Acad. Sci. U.S.A., 2019-10-14;116(44):22014-22019.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging
    Authors: DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé
    JCI Insight, 2019-06-11;5(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
    Authors: EJ Im, CH Lee, PG Moon, GG Rangaswamy, B Lee, JM Lee, JC Lee, JG Jee, JS Bae, TK Kwon, KW Kang, MS Jeong, JE Lee, HS Jung, HJ Ro, S Jun, W Kang, SY Seo, YE Cho, BJ Song, MC Baek
    Nat Commun, 2019-03-27;10(1):1387.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Locally anchoring enzymes to tissues via extracellular glycan recognition
    Authors: SA Farhadi, E Bracho-San, MM Fettis, DT Seroski, SL Freeman, A Restuccia, BG Keselowsky, GA Hudalla
    Nat Commun, 2018-11-22;9(1):4943.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Effects of pepsin and pepstatin on reflux tonsil hypertrophy in vitro
    Authors: JH Kim, SJ Jang, JW Yun, MH Jung, SH Woo
    PLoS ONE, 2018-11-08;13(11):e0207090.
    Species: Human
    Sample Types: Whole Cells
  30. VSIG-3 as a ligand of VISTA inhibits human T-cell function.
    Authors: Wang J, Wu G, Manick B, Hernandez V, Renelt M, Erickson C, Guan J, Singh R, Rollins S, Solorz A, Bi M, Li J, Grabowski D, Dirkx J, Tracy C, Stuart T, Ellinghuysen C, Desmond D, Foster C, Kalabokis V
    Immunology, 2018-10-10;156(1):74-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Altered levels of memory T cell subsets and common ?c cytokines in Strongyloides stercoralis infection and partial reversal following anthelmintic treatment
    Authors: A Rajamanick, S Munisankar, Y Bhootra, CK Dolla, K Thiruvenga, TB Nutman, S Babu
    PLoS Negl Trop Dis, 2018-05-24;12(5):e0006481.
    Species: Human
    Sample Types: Plasma
  32. Anti-inflammatory cytokine and angiogenic factors levels in vitreous samples of diabetic retinopathy patients
    Authors: T Tsai, S Kuehn, N Tsiampalis, MK Vu, V Kakkassery, G Stute, HB Dick, SC Joachim
    PLoS ONE, 2018-03-27;13(3):e0194603.
    Species: Human
    Sample Types: Vitreous Humor
  33. Occupational exposure to ultrafine particles in police officers: no evidence for adverse respiratory effects
    Authors: G Jordakieva, I Grabovac, E Valic, KE Schmidt, A Graff, A Schuster, K Hoffmann-S, C Oberhuber, O Scheiner, A Goll, J Godnic-Cva
    J Occup Med Toxicol, 2018-02-03;13(0):5.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture
  34. Construction, Expression, and Characterization of rSEA-EGF and In Vitro Evaluation of its Antitumor Activity Against Nasopharyngeal Cancer
    Authors: X Liu, L Zeng, Z Zhao, Y Xie, S Wang, J Zhang, Y He, Z Zou, J Zhang, A Tao
    Technol. Cancer Res. Treat., 2018-01-01;17(0):1533033818762.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. The Effects of Perioperative Anesthesia and Analgesia on Immune Function in Patients Undergoing Breast Cancer Resection: A Prospective Randomized Study
    Authors: JS Cho, MH Lee, SI Kim, S Park, HS Park, E Oh, JH Lee, BN Koo
    Int J Med Sci, 2017-08-18;14(10):970-976.
    Species: Human
    Sample Types: Serum
  36. Infiltrating mast cells enhance benign prostatic hyperplasia through IL-6/STAT3/Cyclin D1 signals
    Authors: Z Ou, Y He, L Qi, X Zu, L Wu, Z Cao, Y Li, L Liu, DA Dube, Z Wang, L Wang
    Oncotarget, 2017-07-22;8(35):59156-59164.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. Monocyte chemoattractant protein-1 polymorphism interaction with spirulina immunomodulatory effects in healthy Korean elderly: A 16 week, double-blind randomized clinical trial
    Authors: HJ Park, HS Lee
    Nutr Res Pract, 2017-06-09;11(4):290-299.
    Species: Human
    Sample Types: Plasma
  38. Abnormal gene and protein expression of inflammatory cytokines in the postmortem brain of schizophrenia patients
    Authors: GN Pandey, HS Rizavi, H Zhang, X Ren
    Schizophr. Res., 2017-05-02;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
  39. Diminished plasma levels of common ?-chain cytokines in pulmonary tuberculosis and reversal following treatment
    Authors: NP Kumar, VV Banurekha, D Nair, S Babu
    PLoS ONE, 2017-04-27;12(4):e0176495.
    Species: Human
    Sample Types: Plasma
  40. T Cell Subset and Stimulation Strength-Dependent Modulation of T Cell Activation by Kv1.3 Blockers
    Authors: WP Fung-Leung, W Edwards, Y Liu, K Ngo, J Angsana, G Castro, N Wu, X Liu, RV Swanson, AD Wickenden
    PLoS ONE, 2017-01-20;12(1):e0170102.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Mechanism of action and effect of immune-modulating agents in the treatment of psoriasis
    Authors: Rehab M El-Gharaba
    Biomed. Pharmacother, 2016-12-05;85(0):141-147.
    Species: Human
    Sample Types: Serum
  42. Human amniotic epithelial cells inhibit CD4+ T cell activation in acute kidney injury patients by influencing the miR-101-c-Rel-IL-2 pathway
    Authors: Junfeng Liu
    Mol. Immunol, 2016-11-26;81(0):76-84.
    Species: Human
    Sample Types: Cell Lysates
  43. The Suppressed Induction of Human Mature Cytotoxic T Lymphocytes Caused by Asbestos Is Not due to Interleukin-2 Insufficiency
    J Immunol Res, 2016-11-15;2016(0):7484872.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Involvement of NK Cells and NKp30 Pathway in Antisynthetase Syndrome
    J Immunol, 2016-08-10;197(5):1621-30.
    Species: Human
    Sample Types: Serum
  45. Impact of Exogenous Galectin-9 on Human T Cells: CONTRIBUTION OF THE T CELL RECEPTOR COMPLEX TO ANTIGEN-INDEPENDENT ACTIVATION BUT NOT TO APOPTOSIS INDUCTION.
    Authors: Lhuillier C, Barjon C, Niki T, Gelin A, Praz F, Morales O, Souquere S, Hirashima M, Wei M, Dellis O, Busson P
    J Biol Chem, 2015-05-06;290(27):16797-811.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. The Association between Serum Cytokines and Damage to Large and Small Nerve Fibers in Diabetic Peripheral Neuropathy.
    Authors: Magrinelli F, Briani C, Romano M, Ruggero S, Toffanin E, Triolo G, Peter G, Praitano M, Lauriola M, Zanette G, Tamburin S
    J Diabetes Res, 2015-04-16;2015(0):547834.
    Species: Human
    Sample Types: Serum
  47. Inhibition of G-protein betagamma signaling enhances T cell receptor-stimulated interleukin 2 transcription in CD4+ T helper cells.
    Authors: Yost E, Hynes T, Hartle C, Ott B, Berlot C
    PLoS ONE, 2015-01-28;10(1):e0116575.
    Species: Human
    Sample Types: Cell Culture Supernates
  48. The injury-induced myokine insulin-like 6 is protective in experimental autoimmune myositis.
    Authors: Zeng L, Maruyama S, Nakamura K, Parker-Duffen J, Adham I, Zhong X, Lee H, Querfurth H, Walsh K
    Skelet Muscle, 2014-08-04;4(0):16.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. Role of programmed death ligands in effective T-cell interactions in extranodal natural killer/T-cell lymphoma.
    Authors: Han L, Liu F, Li R, Li Z, Chen X, Zhou Z, Zhang X, Hu T, Zhang Y, Young K, Sun S, Wen J, Zhang M
    Oncol Lett, 2014-07-17;8(4):1461-1469.
    Species: Human
    Sample Types: Serum
  50. Targeting the ion channel Kv1.3 with scorpion venom peptides engineered for potency, selectivity, and half-life.
    Authors: Edwards W, Fung-Leung W, Huang C, Chi E, Wu N, Liu Y, Maher M, Bonesteel R, Connor J, Fellows R, Garcia E, Lee J, Lu L, Ngo K, Scott B, Zhou H, Swanson R, Wickenden A
    J Biol Chem, 2014-06-17;289(33):22704-14.
    Species: Human
    Sample Types: Cell Culture Supernates
  51. Activation of silenced cytokine gene promoters by the synergistic effect of TBP-TALE and VP64-TALE activators.
    Authors: Anthony, Kim, More, Abhijit, Zhang, Xiaoliu
    PLoS ONE, 2014-04-22;9(4):e95790.
    Species: Human
    Sample Types: Cell Culture Supernates
  52. Sos1 regulates sustained TCR-mediated Erk activation.
    Authors: Poltorak M, Meinert I, Stone J, Schraven B, Simeoni L
    Eur J Immunol, 2014-02-20;44(5):1535-40.
    Species: Human
    Sample Types: Cell Culture Supernates
  53. Immunogenicity of a vaccine regimen composed of simian immunodeficiency virus DNA, rMVA, and viral particles administered to female rhesus macaques via four different mucosal routes.
    Authors: Manrique M, Kozlowski P, Cobo-Molinos A, Wang S, Wilson R, Montefiori D, Carville A, Aldovini A
    J Virol, 2013-02-13;87(8):4738-50.
    Species: Human
    Sample Types: Cell Culture Supernates
  54. Multiplexing immunoassays for cytokine detection in the serum of patients with rheumatoid arthritis: lack of sensitivity and interference by rheumatoid factor.
    Authors: Churchman SM, Geiler J, Parmar R, Horner EA, Church LD, Emery P, Buch MH, McDermott MF, Ponchel F
    Clin. Exp. Rheumatol., 2012-08-29;30(4):534-42.
    Species: Human
    Sample Types: Serum
  55. Origination of new immunological functions in the costimulatory molecule B7-H3: the role of exon duplication in evolution of the immune system.
    Authors: Sun J, Fu F, Gu W, Yan R, Zhang G, Shen Z, Zhou Y, Wang H, Shen B, Zhang X
    PLoS ONE, 2011-09-13;6(9):e24751.
    Species: Human
    Sample Types: Cell Culture Supernates
  56. Antigen presentation and MHC class II expression by human esophageal epithelial cells: role in eosinophilic esophagitis.
    Authors: Mulder DJ, Pooni A, Mak N, Hurlbut DJ, Basta S, Justinich CJ
    Am. J. Pathol., 2011-02-01;178(2):744-53.
    Species: Human
    Sample Types: Cell Culture Supernates
  57. HDAC inhibition by LBH589 affects the phenotype and function of human myeloid dendritic cells.
    Authors: Song W, Tai YT, Tian Z, Hideshima T, Chauhan D, Nanjappa P, Exley MA, Anderson KC, Munshi NC
    Leukemia, 2010-11-19;25(1):161-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  58. Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
    Authors: Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G
    Transplantation, 2009-11-15;88(9):1109-16.
    Species: Human
    Sample Types: Urine
  59. Immunologic markers as predictors of tuberculosis-associated immune reconstitution inflammatory syndrome in HIV and tuberculosis coinfected persons in Thailand.
    Authors: Tieu HV, Ananworanich J, Avihingsanon A, Apateerapong W, Sirivichayakul S, Siangphoe U, Klongugkara S, Boonchokchai B, Hammer SM, Manosuthi W
    AIDS Res. Hum. Retroviruses, 2009-11-01;25(11):1083-9.
    Species: Human
    Sample Types: Serum
  60. CD200 is induced by ERK and is a potential therapeutic target in melanoma.
    Authors: Petermann KB, Rozenberg GI, Zedek D, Groben P, McKinnon K, Buehler C, Kim WY, Shields JM, Penland S, Bear JE, Thomas NE, Serody JS, Sharpless NE
    J. Clin. Invest., 2007-12-01;117(12):3922-3929.
    Species: Human
    Sample Types: Cell Culture Supernates
  61. 1, 25 Dihydroxyvitamin D3 modulated cytokine response in pulmonary tuberculosis.
    Authors: Vidyarani M, Selvaraj P, Jawahar MS, Narayanan PR
    Cytokine, 2007-10-29;40(2):128-34.
    Species: Human
    Sample Types: Cell Culture Supernates
  62. Short communication: decreasing soluble CD30 and increasing IFN-gamma plasma levels are indicators of effective highly active antiretroviral therapy.
    Authors: Sadeghi M, Susal C, Daniel V, Naujokat C, Zimmermann R, Huth-Kuhne A, Opelz G
    AIDS Res. Hum. Retroviruses, 2007-07-01;23(7):886-90.
    Species: Human
    Sample Types: Plasma
  63. Co-occurrence of IgA antibodies against ethanol metabolites and tissue transglutaminase in alcohol consumers: correlation with proinflammatory cytokines and markers of fibrogenesis.
    Authors: Koivisto H, Hietala J, Anttila P, Niemela O
    Dig. Dis. Sci., 2007-06-28;53(2):500-5.
    Species: Human
    Sample Types: Serum
  64. Functional polymorphisms in FAS and FASL contribute to increased apoptosis of tumor infiltration lymphocytes and risk of breast cancer.
    Authors: Zhang B, Sun T, Xue L, Han X, Zhang B, Lu N, Shi Y, Tan W, Zhou Y, Zhao D, Zhang X, Guo Y, Lin D
    Carcinogenesis, 2006-12-20;28(5):1067-73.
    Species: Human
    Sample Types: Cell Culture Supernates
  65. Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
    Authors: Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S
    Gut, 2006-07-13;56(2):243-52.
    Species: Human
    Sample Types: Cell Culture Supernates
  66. Interleukin-2 in human milk: a potential modulator of lymphocyte development in the breastfed infant.
    Authors: Bryan DL, Forsyth KD, Gibson RA, Hawkes JS
    Cytokine, 2006-04-11;33(5):289-93.
    Species: Human
    Sample Types: Milk
  67. Expression of B7-H1 on gastric epithelial cells: its potential role in regulating T cells during Helicobacter pylori infection.
    Authors: Das S, Suarez G, Beswick EJ, Sierra JC, Graham DY, Reyes VE
    J. Immunol., 2006-03-01;176(5):3000-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  68. Influence of eotaxin 67G>A polymorphism on plasma eotaxin concentrations in myocardial infarction survivors and healthy controls.
    Authors: Sheikine Y, Olsen B, Gharizadeh B, Jatta K, Tornvall P, Ghaderi M
    Atherosclerosis, 2006-02-28;189(2):458-63.
    Species: Human
    Sample Types: Serum
  69. C-reactive protein and vascular cell adhesion molecule-1 as markers of severity in sickle cell disease.
    Authors: Makis AC, Hatzimichael EC, Stebbing J, Bourantas KL
    Arch. Intern. Med., 2006-02-13;166(3):366-8.
    Species: Human
    Sample Types: Plasma
  70. Decreased plasma CXCL16/SR-PSOX concentration is associated with coronary artery disease.
    Authors: Sheikine Y, Bang CS, Nilsson L, Samnegard A, Hamsten A, Jonasson L, Eriksson P, Sirsjo A
    Atherosclerosis, 2005-12-27;188(2):462-6.
    Species: Human
    Sample Types: Serum
  71. Primary cutaneous T-cell lymphomas show a deletion or translocation affecting NAV3, the human UNC-53 homologue.
    Authors: Karenko L, Hahtola S, Paivinen S, Karhu R, Syrja S, Kahkonen M, Nedoszytko B, Kytölä S, Zhou Y, Blazevic V, Pesonen M, Nevala H, Nupponen N, Sihto H, Krebs I, Poustka A, Roszkiewicz J, Saksela K, Peterson P, Visakorpi T, Ranki A
    Cancer Res., 2005-09-15;65(18):8101-10.
    Species: Human
    Sample Types: Cell Culture Supernates
  72. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
    Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
    Microbes Infect., 2005-02-24;7(3):427-36.
    Species: Human
    Sample Types: Serum
  73. Strong inflammatory cytokine response in male and strong anti-inflammatory response in female kidney transplant recipients with urinary tract infection.
    Authors: Sadeghi M, Daniel V, Naujokat C, Wiesel M, Hergesell O, Opelz G
    Transpl. Int., 2005-02-01;18(2):177-85.
    Species: Human
    Sample Types: Plasma
  74. Cytokines and adhesion molecules in multiple sclerosis patients treated with interferon-beta1b.
    Authors: Jensen J, Krakauer M, Sellebjerg F
    Cytokine, 2005-01-07;29(1):24-30.
    Species: Human
    Sample Types: Plasma
  75. Inflammatory cytokine concentrations are elevated in seminal plasma of men with spinal cord injuries.
    Authors: Basu S, Aballa TC, Ferrell SM, Lynne CM, Brackett NL
    J. Androl., 2004-03-01;25(2):250-4.
    Species: Human
    Sample Types: Seminal Plasma
  76. Urinary IL-2 assay for monitoring intravesical bacillus Calmette-Guerin response of superficial bladder cancer during induction course and maintenance therapy.
    Authors: Saint F, Kurth N, Maille P, Vordos D, Hoznek A, Soyeux P, Patard JJ, Abbou CC, Chopin DK
    Int. J. Cancer, 2003-11-10;107(3):434-40.
    Species: Human
    Sample Types: Urine
  77. Intraperitoneal bispecific antibody (HEA125xOKT3) therapy inhibits malignant ascites production in advanced ovarian carcinoma.
    Authors: Marme A, Strauss G, Bastert G, Grischke EM, Moldenhauer G
    Int. J. Cancer, 2002-09-10;101(2):183-9.
    Species: Human
    Sample Types: Ascites Fluid
  78. Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15.
    Authors: Briard D, Brouty-Boye D, Azzarone B, Jasmin C
    J. Immunol., 2002-05-01;168(9):4326-32.
    Species: Human
    Sample Types: Cell Culture Supernates
  79. Embryotoxicity of peritoneal fluid in women with endometriosis. Its relation with cytokines and lymphocyte populations.
    Authors: Gomez-Torres MJ, Acien P, Campos A, Velasco I
    Hum. Reprod., 2002-03-01;17(3):777-81.
    Species: Human
    Sample Types: Peritoneal Fluid
  80. Local immunostimulation induced by intravesical administration of autologous interferon-gamma-activated macrophages in patients with superficial bladder cancer.
    Authors: Pages F, Lebel-Binay S, Vieillefond A, Deneux L, Cambillau M, Soubrane O, Debre B, Tardy D, Lemonne JL, Abastado JP, Fridman WH, Thiounn N
    Clin. Exp. Immunol., 2002-02-01;127(2):303-9.
    Species: Human
    Sample Types: Urine
  81. Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor.
    Authors: Bovolenta C, Pilotti E, Mauri M, Turci M, Ciancianaini P, Fisicaro P, Bertazzoni U, Poli G, Casoli C
    Blood, 2002-01-01;99(1):224-31.
    Species: Human
    Sample Types: Cell Culture Supernates
  82. An analysis of acute changes in interleukin-6 levels after treatment of hepatitis C with consensus interferon.
    Authors: Cotler SJ, Reddy KR, McCone J, Wolfe DL, Liu A, Craft TR, Ferris MW, Conrad AJ, Albrecht J, Morrissey M, Ganger DR, Rosenblate H, Blatt LM, Jensen DM, Taylor MW
    J. Interferon Cytokine Res., 2001-12-01;21(12):1011-9.
    Species: Human
    Sample Types: Serum
  83. Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
    Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
    J. Exp. Med., 2000-07-17;192(2):219-26.
    Species: Human
    Sample Types: Cell Culture Supernates
  84. Cytokine profiles in parotid saliva from HIV-1-infected individuals: changes associated with opportunistic infections in the oral cavity.
    Authors: Black, K P, Merrill, K W, Jackson, S, Katz, J
    Oral Microbiol Immunol, 2000-04-01;15(2):74-81.
    Species: Human
    Sample Types: Saliva
  85. Human amniotic fluid lacks interleukin-2 and interleukin-15 but can interact with the beta-chain of the interleukin-2 receptor.
    Authors: Searle RF, Bromage SJ, Palmer J, Curry JE, Lang AK
    Immunology, 2000-03-01;99(3):411-7.
    Species: Human
    Sample Types: Amniotic Fluid
  86. Effects of UVB radiation on cytokine generation, cell adhesion molecules, and cell activation markers in T-lymphocytes and peripheral blood HPCs.
    Authors: Lankford KV, Mosunjac M, Hillyer CD
    Transfusion, 2000-03-01;40(3):361-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  87. High versus low basal cortisol secretion in asymptomatic, medication-free HIV-infected men: differential effects of severe life stress on parameters of immune status.
    Authors: Petitto JM, Leserman J, Perkins DO, Stern RA, Silva SG, Gettes D, Zheng B, Folds JD, Golden RN, Evans DL
    Behav Med, 2000-01-01;25(4):143-51.
    Species: Human
    Sample Types: Cell Culture Supernates

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Reviews for Human IL-2 Quantikine ELISA Kit

Average Rating: 4.7 (Based on 25 Reviews)

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Human IL-2 Quantikine ELISA Kit
By Anonymous on 04/03/2023
Sample Tested: Cell Culture Samples

Human IL-2 Quantikine ELISA Kit
By Anonymous on 02/27/2023
Sample Tested: Serum and Plasma

Human IL-2 Quantikine ELISA Kit
By Anonymous on 02/25/2023
Sample Tested: Whole blood T cells

Human IL-2 Quantikine ELISA Kit
By Anonymous on 06/14/2022
Sample Tested: Serum

Human IL-2 Quantikine ELISA Kit
By Anonymous on 06/07/2022
Sample Tested: Serum

Human IL-2 Quantikine ELISA Kit
By Anonymous on 09/28/2021
Sample Tested: Cell culture supernatant

Human IL-2 Quantikine ELISA Kit
By Anonymous on 03/16/2021
Sample Tested: Peripheral blood mononuclear cells (PBMCs)

Human IL-2 Quantikine ELISA Kit
By Anonymous on 12/20/2020
Sample Tested: Plasma

Human IL-2 Quantikine ELISA Kit
By Vijay Patel on 12/12/2020
Sample Tested: Serum

Human IL-2 Quantikine ELISA Kit
By Mike Ehrhardt on 11/24/2020
Sample Tested: Serum-free Cell Culture Supernates

This ELISA performed as expected with low % CV's


Human IL-2 Quantikine ELISA Kit
By Anonymous on 10/19/2020
Sample Tested: Product medium matrix

Human IL-2 Quantikine ELISA Kit
By Thanh Nguyen on 10/18/2020
Sample Tested: Blood monocytes

Human IL-2 Quantikine ELISA Kit
By Anonymous on 10/12/2020
Sample Tested: Serum and Plasma

Great assay that is easy to use and provides correct results. The typical range for healthy patients is very low, this test shows that accurately.


Human IL-2 Quantikine ELISA Kit
By Anonymous on 01/06/2020
Sample Tested: Serum-free Cell Culture Media

Human IL-2 Quantikine ELISA Kit
By Anonymous on 12/08/2019
Sample Tested: Cell culture supernatant

Human IL-2 Quantikine ELISA Kit
By Anonymous on 12/08/2019
Sample Tested: Cell culture supernatant

Human IL-2 Quantikine ELISA Kit
By Dhivya Sridaran on 04/04/2019
Sample Tested: Jurkat human acute T cell leukemia cell line

Human IL-2 Quantikine ELISA Kit
By Dhivya Sridaran on 04/04/2019
Sample Tested: MOLT-4 human acute lymphoblastic leukemia cell line

Human IL-2 Quantikine ELISA Kit
By Anonymous on 03/21/2019
Sample Tested: Jurkat human acute T cell leukemia cell line,MOLT-4 human acute lymphoblastic leukemia cell line

Human IL-2 Quantikine ELISA Kit
By Shambhavi Bhagwat on 03/21/2019
Sample Tested: A375 human melanoma cell line

Human IL-2 Quantikine ELISA Kit
By Anonymous on 11/02/2018
Sample Tested: Serum

Human IL-2 Quantikine ELISA Kit
By Anonymous on 04/04/2018
Sample Tested: Serum and Plasma

Human IL-2 Quantikine ELISA Kit
By Leslie Priddy on 03/30/2018
Sample Tested: Serum

Human IL-2 Quantikine ELISA Kit
By Gongxian Liao on 03/22/2018
Sample Tested: Human PBMC after stimulation in culture

Human IL-2 Quantikine ELISA Kit
By Anonymous on 11/03/2017
Sample Tested: mouse serum

Used mice treated with human IL-2 product to test the IL-2 clearance in the blood.
Were able to use our own IL-2 construct to make a trustable standard curve and comparable with the kit's products.

We use this kit daily.